A0RHY6 (A0RHY6_BACAH) Unreviewed, UniProtKB/TrEMBL

Names and origin

Protein names

Recommended name:
Peptide deformylase 2 HAMAP MF_00163
Short name=PDF 2 HAMAP MF_00163
EC=3.5.1.88 HAMAP MF_00163

Alternative name(s):
Polypeptide deformylase 2 HAMAP MF_00163

Gene names

Name:	def2 HAMAP MF_00163
Ordered Locus Names:	BALH_3596

Organism

Bacillus thuringiensis (strain Al Hakam) [Complete proteome] [HAMAP]

Taxonomic identifier

412694 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Bacillaceae › Bacillus › Bacillus cereus group

Protein attributes

Sequence length	184 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Removes the formyl group from the N-terminal Met of newly synthesized proteins. Requires at least a dipeptide for an efficient rate of reaction. N-terminal L-methionine is a prerequisite for activity but the enzyme has broad specificity at other positions By similarity. HAMAP MF_00163
Catalytic activity	Formyl-L-methionyl peptide + H2O = formate + methionyl peptide. HAMAP MF_00163 SAAS SAAS000181
Cofactor	Binds 1 Fe2+ ion By similarity. HAMAP MF_00163
Sequence similarities	Belongs to the polypeptide deformylase family. HAMAP MF_00163 RuleBase RU003335

Ontologies

Keywords
Biological process	Protein biosynthesis HAMAP MF_00163 SAAS SAAS000181
Ligand	Iron HAMAP MF_00163 Metal-binding HAMAP MF_00163 SAAS SAAS000181
Molecular function	Hydrolase HAMAP MF_00163 SAAS SAAS000181
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	translation Inferred from electronic annotation. Source: HAMAP
Molecular function	iron ion binding Inferred from electronic annotation. Source: InterPro peptide deformylase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Sites
	Active site	154	1	By similarity HAMAP MF_00163
	Metal binding	110	1	Iron By similarity HAMAP MF_00163
	Metal binding	153	1	Iron By similarity HAMAP MF_00163
	Metal binding	157	1	Iron By similarity HAMAP MF_00163

Sequences

	Sequence		Length	Mass (Da)
	A0RHY6 [UniParc]. Last modified January 9, 2007. Version 1. Checksum: E7605C5E3201021E	FASTA	184	20,542
	10 20 30 40 50 60 MLTMKDVIRE GDPILRNVAE EVVIPASEED TNTLKEMIEF VINSQDPEMA EKYSLRPGIG 70 80 90 100 110 120 LAAPQIGISK KMIAVHVTDT DGTLYSYALF NPKIISHSVE RTYLQSGEGC LSVDREVPGY 130 140 150 160 170 180 VPRYTRITVK ATSINGEEVK LRLKGLPAIV FQHEIDHLNG VMFYDHINKE NPFAAPDGSK PLER

References

[1]

"The complete genome sequence of Bacillus thuringiensis Al Hakam." Challacombe J.F., Altherr M.R., Xie G., Bhotika S.S., Brown N., Bruce D., Campbell C.S., Campbell M.L., Chen J., Chertkov O., Cleland C., Dimitrijevic M., Doggett N.A., Fawcett J.J., Glavina T., Goodwin L.A., Green L.D., Han C.S. , Hill K.K., Hitchcock P., Jackson P.J., Keim P., Kewalramani A.R., Longmire J., Lucas S., Malfatti S., Martinez D., McMurry K., Meincke L.J., Misra M., Moseman B.L., Mundt M., Munk A.C., Okinaka R.T., Parson-Quintana B., Reilly L.P., Richardson P., Robinson D.L., Saunders E., Tapia R., Tesmer J.G., Thayer N., Thompson L.S., Tice H., Ticknor L.O., Wills P.L., Gilna P., Brettin T.S. J. Bacteriol. 189:3680-3681(2007) [PubMed: 17337577] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000485 Genomic DNA. Translation: ABK86829.1.
RefSeq	YP_896336.1. NC_008600.1.
3D structure databases
ProteinModelPortal	A0RHY6.
SMR	A0RHY6. Positions 1-184.
ModBase	Search...
Protein-protein interaction databases
STRING	A0RHY6.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBBACT00000070434; EBBACP00000068671; EBBACG00000070425.
GeneID	4543738.
GenomeReviews	Gene locus BALH_3596 in contig CP000485_GR.
KEGG	btl:BALH_3596.
NMPDR	fig|412694.5.peg.3457.
PATRIC	18999967. VBIBacThu63319_3983.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0242.
GeneTree	EBGT00050000001175.
HOGENOM	HBG665227.
OMA	ADGEGCL.
ProtClustDB	PRK00150.
Family and domain databases
HAMAP	MF_00163. Pep_deformylase. [Tree]
InterPro	IPR000181. Fmet_deformylase. IPR023635. Peptide_deformylase. [Graphical view]
Gene3D	G3DSA:3.90.45.10. Fmet_deformylase. 1 hit.
KO	K01462.
PANTHER	PTHR10458. Fmet_deformylase. 1 hit.
Pfam	PF01327. Pep_deformylase. 1 hit. [Graphical view]
PIRSF	PIRSF004749. Pep_def. 1 hit.
PRINTS	PR01576. PDEFORMYLASE.
SUPFAM	SSF56420. Fmet_deformylase. 1 hit.
TIGRFAMs	TIGR00079. Pept_deformyl. 1 hit.
ProtoNet	Search...

Entry information

Entry name

A0RHY6_BACAH

Accession

Primary (citable) accession number: A0RHY6

Entry history

Integrated into UniProtKB/TrEMBL:	January 9, 2007
Last sequence update:	January 9, 2007
Last modified:	December 14, 2011
This is version 36 of the entry and version 1 of the sequence. [Complete history]

Entry status

Unreviewed (UniProtKB/TrEMBL)