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A0RHK6 (A0RHK6_BACAH) Unreviewed, UniProtKB/TrEMBL

Last modified December 14, 2011. Version 45. Feed History...

Clusters with 100%, 90%, 50% identity | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
DNA topoisomerase RuleBase RU000518
EC=5.99.1.2 RuleBase RU000518
Gene names
Ordered Locus Names:BALH_3464
OrganismBacillus thuringiensis (strain Al Hakam) [Complete proteome] [HAMAP]
Taxonomic identifier412694 [NCBI]
Taxonomic lineageBacteria › Firmicutes › Bacillales › Bacillaceae › Bacillus › Bacillus cereus group

Protein attributes

Sequence length692 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Releases the supercoiling and torsional tension of DNA introduced during the DNA replication and transcription by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(5'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 3'-OH DNA strand. The free DNA strand than undergoes passage around the unbroken strand thus removing DNA supercoils. Finally, in the religation step, the DNA 3'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone By similarity. SAAS SAAS000380

Catalytic activity

ATP-independent breakage of single-stranded DNA, followed by passage and rejoining. RuleBase RU000518 SAAS SAAS005733

Sequence similarities

Belongs to the prokaryotic type I/III topoisomerase family. RuleBase RU004206

Contains 1 Toprim domain. SAAS SAAS023406

Sequences

Sequence LengthMass (Da)Tools
A0RHK6 [UniParc].

Last modified January 9, 2007. Version 1.
Checksum: 9EBA587C6F59848E

FASTA69279,064
        10         20         30         40         50         60 
MSDYLVIVES PSKAKTIEKY LGKKYKVVAS MGHVRDLPKS QMGIEVKNNF TPKYITIRGK 

        70         80         90        100        110        120 
GPVLKDLKSA AKKAKKVYLA ADPDREGEAI AWHLANTLNV DVESDCRVVF NEITKDAIKE 

       130        140        150        160        170        180 
SFKHPRAINM DLVDAQQARR ILDRLVGYNI SPLLWKKVKK GLSAGRVQSV AVRLIIERER 

       190        200        210        220        230        240 
EIQSFEPEEF WTIKTEFVKG KDTFEASFYG VDGEKVQLTN ETQVNEIIEQ LKDNAFSVEN 

       250        260        270        280        290        300 
VTRKERKRNP ALPFTTSSLQ QEAARKLNMR AKKTMMLAQQ LYEGIDLGKQ GTVGLITYMR 

       310        320        330        340        350        360 
TDSTRISETA QTEARTYITE AYGTEYIGAE KKKETKKSNA QDAHEAIRPT SVMRKPEELK 

       370        380        390        400        410        420 
SFLSRDQLRL YKLIWERFVA SQMASAIMDT VTARLINNNV QFRASGSVVK FPGFMKVYVE 

       430        440        450        460        470        480 
SKDDGAEEKD KMLPPLEVGE TVFSKDLEPK QHFTQPPPRY TEARLVRTLE ELGIGRPSTY 

       490        500        510        520        530        540 
VPTLETIQKR GYVGLDNKRF VPTELGEIVI ELILEFFPEI INIEFTANME QSLDEVEEGN 

       550        560        570        580        590        600 
ANWVKIVDDF YVGFEPRLEK AEKEMREVEI KDEPAGEDCE LCNHPMVFKM GKYGKFMACS 

       610        620        630        640        650        660 
NFPDCRNTKP IVKEIGVTCP KCDKGQIIER RSNKKKRLFY GCGTYPECDF VSWDKPIGRK 

       670        680        690 
CPKCEGMLVE KKLKKGVQVQ CISCDYEEEQ QM

« Hide

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		CP000485 Genomic DNA. Translation: ABK86699.1.
RefSeqYP_896206.1. NC_008600.1.

3D structure databases

ProteinModelPortalA0RHK6.
ModBaseSearch...

Protein-protein interaction databases

STRINGA0RHK6.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaEBBACT00000069873; EBBACP00000068110; EBBACG00000069864.
GeneID4543250.
GenomeReviewsGene locus BALH_3464 in contig CP000485_GR.
KEGGbtl:BALH_3464.
PATRIC18999679. VBIBacThu63319_3839.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0550.
GeneTreeEBGT00070000032133.
HOGENOMHBG512935.
OMAEEYWSID.
ProtClustDBPRK05582.

Family and domain databases

InterProIPR000380. Topo_IA.
IPR003601. Topo_IA_2.
IPR023406. Topo_IA_AS.
IPR013497. Topo_IA_cen.
IPR013824. Topo_IA_cen_sub1.
IPR013825. Topo_IA_cen_sub2.
IPR023405. Topo_IA_core_domain.
IPR003602. Topo_IA_DNA-bd.
IPR013498. Topo_IA_Znf.
IPR005733. TopoI_bac-type.
IPR006171. Toprim_domain.
[Graphical view]
Gene3DG3DSA:1.10.460.10. Topo_IA_cen_sub1. 2 hits.
G3DSA:2.70.20.10. Topo_IA_cen_sub2. 2 hits.
KOK03168.
PANTHERPTHR11390. Topo_IA. 1 hit.
PfamPF01131. Topoisom_bac. 1 hit.
PF01751. Toprim. 1 hit.
PF01396. zf-C4_Topoisom. 2 hits.
[Graphical view]
PRINTSPR00417. PRTPISMRASEI.
SMARTSM00437. TOP1Ac. 1 hit.
SM00436. TOP1Bc. 1 hit.
SM00493. TOPRIM. 1 hit.
[Graphical view]
SUPFAMSSF56712. Topo_IA_core. 1 hit.
TIGRFAMsTIGR01051. TopA_bact. 1 hit.
PROSITEPS00396. TOPOISOMERASE_I_PROK. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameA0RHK6_BACAH
AccessionPrimary (citable) accession number: A0RHK6
Entry history
Integrated into UniProtKB/TrEMBL: January 9, 2007
Last sequence update: January 9, 2007
Last modified: December 14, 2011
This is version 45 of the entry and version 1 of the sequence. [Complete history]
Entry statusUnreviewed (UniProtKB/TrEMBL)
to top of pageNames·Attributes·General annotation·Ontologies·Sequences·References·Cross-refs·Entry info