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A0RHK5 (TRMFO_BACAH) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 57. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Methylenetetrahydrofolate--tRNA-(uracil-5-)-methyltransferase TrmFO

EC=2.1.1.74
Alternative name(s):
Folate-dependent tRNA (uracil-5-)-methyltransferase
Folate-dependent tRNA(M-5-U54)-methyltransferase
Gene names
Name:trmFO
Ordered Locus Names:BALH_3463
OrganismBacillus thuringiensis (strain Al Hakam) [Complete proteome] [HAMAP]
Taxonomic identifier412694 [NCBI]
Taxonomic lineageBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillusBacillus cereus group

Protein attributes

Sequence length434 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the folate-dependent formation of 5-methyl-uridine at position 54 (M-5-U54) in all tRNAs By similarity. HAMAP-Rule MF_01037

Catalytic activity

5,10-methylenetetrahydrofolate + uracil54 in tRNA + FADH2 = tetrahydrofolate + 5-methyluracil54 in tRNA + FAD. HAMAP-Rule MF_01037

Cofactor

FAD By similarity. HAMAP-Rule MF_01037

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_01037.

Sequence similarities

Belongs to the MnmG family. TrmFO subfamily.

Sequence caution

The sequence ABK86698.1 differs from that shown. Reason: Erroneous initiation.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 434434Methylenetetrahydrofolate--tRNA-(uracil-5-)-methyltransferase TrmFO HAMAP-Rule MF_01037
PRO_0000346320

Regions

Nucleotide binding10 – 156FAD By similarity

Sequences

Sequence LengthMass (Da)Tools
A0RHK5 [UniParc].

Last modified September 2, 2008. Version 2.
Checksum: 0A95FBAF5183273D

FASTA43448,052
        10         20         30         40         50         60 
MTTQVVNVIG AGLAGSEAAY QIAKRGVQVR LYEMRPVRQT PAHHTDKFAE LVCSNSLRAN 

        70         80         90        100        110        120 
TLTNAVGVIK EEMRLMDSVI IRAADECSVP AGGALAVDRH EFAAKVTEYV KNHPNVTVMN 

       130        140        150        160        170        180 
EEITEIPEGP TIIATGPLTS PDLSAQLKEL TGEDYFYFYD AAAPIVEKDS IDMNKVYLKS 

       190        200        210        220        230        240 
RYDKGEAAYL NCPMTEEEFD RFYEALIAAE TVPLKEFEKE IFFEGCMPVE VMASRGRQTL 

       250        260        270        280        290        300 
VFGPMKPVGL EDPKTGKTPY AVVQLRQDDA AGTLYNIVGF QTHLKWGPQK EVLQLIPGLE 

       310        320        330        340        350        360 
NAEIVRYGVM HRNTFINSPN LLRPTYQYKQ RDDLFFAGQM TGVEGYVESA ASGLLAGINA 

       370        380        390        400        410        420 
ARLVKGEEPI VLPPVTAMGS MANYITATNA KNFQPMNANF GLFAPLEKKI KKKAERNEAY 

       430 
ATRALETIRN FVNI 

« Hide

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP000485 Genomic DNA. Translation: ABK86698.1. Different initiation.
RefSeqYP_896205.1. NC_008600.1.

3D structure databases

ProteinModelPortalA0RHK5.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING412694.BALH_3463.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaABK86698; ABK86698; BALH_3463.
GeneID4543249.
KEGGbtl:BALH_3463.
PATRIC18999677. VBIBacThu63319_3838.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG1206.
HOGENOMHOG000252054.
KOK04094.
OrthoDBEOG6J74VT.
ProtClustDBPRK05335.

Enzyme and pathway databases

BioCycBTHU412694:GH1W-3391-MONOMER.

Family and domain databases

HAMAPMF_01037. TrmFO.
InterProIPR004417. Folate-dep_Ribothymidyl_synth.
IPR002218. GIDA-rel.
IPR020595. GIDA-rel_CS.
[Graphical view]
PfamPF01134. GIDA. 1 hit.
[Graphical view]
TIGRFAMsTIGR00137. gid_trmFO. 1 hit.
PROSITEPS01281. GIDA_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameTRMFO_BACAH
AccessionPrimary (citable) accession number: A0RHK5
Entry history
Integrated into UniProtKB/Swiss-Prot: September 2, 2008
Last sequence update: September 2, 2008
Last modified: April 16, 2014
This is version 57 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families