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A0RDG3

- SYI_BACAH

UniProt

A0RDG3 - SYI_BACAH

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Protein
Isoleucine--tRNA ligase
Gene
ileS, BALH_1940
Organism
Bacillus thuringiensis (strain Al Hakam)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile) By similarity.UniRule annotation

Catalytic activityi

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

Cofactori

Zinc By similarity.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei593 – 5931ATP By similarity

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-HAMAP
  2. aminoacyl-tRNA editing activity Source: InterPro
  3. isoleucine-tRNA ligase activity Source: UniProtKB-HAMAP
  4. zinc ion binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. isoleucyl-tRNA aminoacylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

BioCyciBTHU412694:GH1W-1948-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Isoleucine--tRNA ligase (EC:6.1.1.5)
Alternative name(s):
Isoleucyl-tRNA synthetase
Short name:
IleRS
Gene namesi
Name:ileS
Ordered Locus Names:BALH_1940
OrganismiBacillus thuringiensis (strain Al Hakam)
Taxonomic identifieri412694 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillusBacillus cereus group
ProteomesiUP000000761: Chromosome

Subcellular locationi

Cytoplasm By similarity UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 10331033Isoleucine--tRNA ligaseUniRule annotation
PRO_1000022147Add
BLAST

Interactioni

Subunit structurei

Monomer By similarity.UniRule annotation

Protein-protein interaction databases

STRINGi412694.BALH_1940.

Structurei

3D structure databases

ProteinModelPortaliA0RDG3.

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi47 – 5711"HIGH" regionUniRule annotation
Add
BLAST
Motifi590 – 5945"KMSKS" regionUniRule annotation

Domaini

IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)) By similarity.UniRule annotation

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0060.
HOGENOMiHOG000246403.
KOiK01870.
OMAiRVEHMVE.
OrthoDBiEOG644ZM1.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_02003. Ile_tRNA_synth_type2.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023586. Ile-tRNA-ligase_type2.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
[Graphical view]
PRINTSiPR00984. TRNASYNTHILE.
SUPFAMiSSF47323. SSF47323. 2 hits.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00392. ileS. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

A0RDG3-1 [UniParc]FASTAAdd to Basket

« Hide

MKKVDVKESA VGRETRIRKQ WNEQSIFEQS IQNREGAQSF VFYEGPPTAN     50
GLPHVGHALG RTIKDVVARY KTMVGYKVLR KAGWDTHGLP VELGVEKQLG 100
ISGKHEIEEY GIEPFIQKCK ESVFTYEKQW REFTESIGYW VDMDDPYVTL 150
ENPYIESVWH ILGTIHEKGL LYKGHRVSPY CPSCQTSLSS HEVAQGYKTV 200
KDLSATVKFE VKDSENEYFL GWTTTPWTLP ANVALAVHPN MEYVKAKQEG 250
HVYIVAKERV QDVLKEDYEV LSVHKGEELV NTSYMPPFPM KEVTNGYHVI 300
AADFVTADSG TGLVHIAPAY GEDDYRVVQS EGLSFLHVVD EKGEYTEAVP 350
FLKGKFVKDC DVDIVRYLAK EGLLYHKEKY EHSYPHCWRC DSPLLYYAGE 400
SWLIRTTAIK DTFLQNNDSV TWYPDHMKHG RFGKFLENMV DWNISRNRYW 450
GTPLNVWECE SCDHQFAPKS IADLRKHSTK ETPEDLELHK PYVDEVQVCC 500
EKCGGTMTRT PEVIDVWFDS GSMPFAQYHY PFENKELFEE QFPADVIAEG 550
IDQTRGWFYS LLAVSALYTG KAPYKRVLSL GHVLDEEGQK MSKSKGNALD 600
PVDLVEKFGA DALRWALLVD SAPWNAKRFS ERTVLEAKSK FVDTLINVYS 650
FYVLYANLDE YNPHETYDVK RTKLDEWVLS RLHSTTKKVR TALDDYQFTN 700
AAREIAALVD EVSNWYVRRS RNRFWESGMN AEKAAAYETL HEVLVTISKL 750
IAPFTPFVAE DIHVNLTGNS VHLEDYPVVN ESLLQPKLEA EMNAVLQVVE 800
LGRSNRNQHS LKVKQPLAEL VLLEHNENDM DWESYRDIVM DELNVKTFHV 850
ELDETKYTSY QLKLNFKKAG PKFGKNVNAV NGWLKQLSQA EVQNFVSTER 900
AVYEVASGEE VVVTAEDVMV EKVAKSGFSN TTNGQYTVML DTNVTEELLQ 950
EGVAREFIRA VQEYRKQLNL PVNLRVDVIL DTEEELQQTL TNHKELLKEN 1000
LLVKQFTFGH LTNEDDELSL GETKVRIKLS SAN 1033
Length:1,033
Mass (Da):118,463
Last modified:January 9, 2007 - v1
Checksum:iD64CDD90BABD2956
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000485 Genomic DNA. Translation: ABK85256.1.
RefSeqiWP_000754935.1. NC_008600.1.
YP_894763.1. NC_008600.1.

Genome annotation databases

EnsemblBacteriaiABK85256; ABK85256; BALH_1940.
GeneIDi4545547.
KEGGibtl:BALH_1940.
PATRICi18996367. VBIBacThu63319_2185.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000485 Genomic DNA. Translation: ABK85256.1 .
RefSeqi WP_000754935.1. NC_008600.1.
YP_894763.1. NC_008600.1.

3D structure databases

ProteinModelPortali A0RDG3.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 412694.BALH_1940.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ABK85256 ; ABK85256 ; BALH_1940 .
GeneIDi 4545547.
KEGGi btl:BALH_1940.
PATRICi 18996367. VBIBacThu63319_2185.

Phylogenomic databases

eggNOGi COG0060.
HOGENOMi HOG000246403.
KOi K01870.
OMAi RVEHMVE.
OrthoDBi EOG644ZM1.

Enzyme and pathway databases

BioCyci BTHU412694:GH1W-1948-MONOMER.

Family and domain databases

Gene3Di 1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPi MF_02003. Ile_tRNA_synth_type2.
InterProi IPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023586. Ile-tRNA-ligase_type2.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view ]
Pfami PF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
[Graphical view ]
PRINTSi PR00984. TRNASYNTHILE.
SUPFAMi SSF47323. SSF47323. 2 hits.
SSF50677. SSF50677. 1 hit.
TIGRFAMsi TIGR00392. ileS. 1 hit.
PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Al Hakam.

Entry informationi

Entry nameiSYI_BACAH
AccessioniPrimary (citable) accession number: A0RDG3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: January 9, 2007
Last modified: September 3, 2014
This is version 60 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi