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A0RDG3

- SYI_BACAH

UniProt

A0RDG3 - SYI_BACAH

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Protein

Isoleucine--tRNA ligase

Gene

ileS

Organism
Bacillus thuringiensis (strain Al Hakam)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile).UniRule annotation

Catalytic activityi

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

Cofactori

Zinc.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei593 – 5931ATPUniRule annotation

GO - Molecular functioni

  1. aminoacyl-tRNA editing activity Source: InterPro
  2. ATP binding Source: UniProtKB-HAMAP
  3. isoleucine-tRNA ligase activity Source: UniProtKB-HAMAP
  4. zinc ion binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. isoleucyl-tRNA aminoacylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

BioCyciBTHU412694:GH1W-1948-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Isoleucine--tRNA ligaseUniRule annotation (EC:6.1.1.5UniRule annotation)
Alternative name(s):
Isoleucyl-tRNA synthetaseUniRule annotation
Short name:
IleRSUniRule annotation
Gene namesi
Name:ileSUniRule annotation
Ordered Locus Names:BALH_1940
OrganismiBacillus thuringiensis (strain Al Hakam)
Taxonomic identifieri412694 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillusBacillus cereus group
ProteomesiUP000000761: Chromosome

Subcellular locationi

Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 10331033Isoleucine--tRNA ligasePRO_1000022147Add
BLAST

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi412694.BALH_1940.

Structurei

3D structure databases

ProteinModelPortaliA0RDG3.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi47 – 5711"HIGH" regionAdd
BLAST
Motifi590 – 5945"KMSKS" region

Domaini

IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)).UniRule annotation

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 2 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiCOG0060.
HOGENOMiHOG000246403.
KOiK01870.
OMAiRVEHMVE.
OrthoDBiEOG644ZM1.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_02003. Ile_tRNA_synth_type2.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023586. Ile-tRNA-ligase_type2.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
[Graphical view]
PRINTSiPR00984. TRNASYNTHILE.
SUPFAMiSSF47323. SSF47323. 2 hits.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00392. ileS. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

A0RDG3-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MKKVDVKESA VGRETRIRKQ WNEQSIFEQS IQNREGAQSF VFYEGPPTAN
60 70 80 90 100
GLPHVGHALG RTIKDVVARY KTMVGYKVLR KAGWDTHGLP VELGVEKQLG
110 120 130 140 150
ISGKHEIEEY GIEPFIQKCK ESVFTYEKQW REFTESIGYW VDMDDPYVTL
160 170 180 190 200
ENPYIESVWH ILGTIHEKGL LYKGHRVSPY CPSCQTSLSS HEVAQGYKTV
210 220 230 240 250
KDLSATVKFE VKDSENEYFL GWTTTPWTLP ANVALAVHPN MEYVKAKQEG
260 270 280 290 300
HVYIVAKERV QDVLKEDYEV LSVHKGEELV NTSYMPPFPM KEVTNGYHVI
310 320 330 340 350
AADFVTADSG TGLVHIAPAY GEDDYRVVQS EGLSFLHVVD EKGEYTEAVP
360 370 380 390 400
FLKGKFVKDC DVDIVRYLAK EGLLYHKEKY EHSYPHCWRC DSPLLYYAGE
410 420 430 440 450
SWLIRTTAIK DTFLQNNDSV TWYPDHMKHG RFGKFLENMV DWNISRNRYW
460 470 480 490 500
GTPLNVWECE SCDHQFAPKS IADLRKHSTK ETPEDLELHK PYVDEVQVCC
510 520 530 540 550
EKCGGTMTRT PEVIDVWFDS GSMPFAQYHY PFENKELFEE QFPADVIAEG
560 570 580 590 600
IDQTRGWFYS LLAVSALYTG KAPYKRVLSL GHVLDEEGQK MSKSKGNALD
610 620 630 640 650
PVDLVEKFGA DALRWALLVD SAPWNAKRFS ERTVLEAKSK FVDTLINVYS
660 670 680 690 700
FYVLYANLDE YNPHETYDVK RTKLDEWVLS RLHSTTKKVR TALDDYQFTN
710 720 730 740 750
AAREIAALVD EVSNWYVRRS RNRFWESGMN AEKAAAYETL HEVLVTISKL
760 770 780 790 800
IAPFTPFVAE DIHVNLTGNS VHLEDYPVVN ESLLQPKLEA EMNAVLQVVE
810 820 830 840 850
LGRSNRNQHS LKVKQPLAEL VLLEHNENDM DWESYRDIVM DELNVKTFHV
860 870 880 890 900
ELDETKYTSY QLKLNFKKAG PKFGKNVNAV NGWLKQLSQA EVQNFVSTER
910 920 930 940 950
AVYEVASGEE VVVTAEDVMV EKVAKSGFSN TTNGQYTVML DTNVTEELLQ
960 970 980 990 1000
EGVAREFIRA VQEYRKQLNL PVNLRVDVIL DTEEELQQTL TNHKELLKEN
1010 1020 1030
LLVKQFTFGH LTNEDDELSL GETKVRIKLS SAN
Length:1,033
Mass (Da):118,463
Last modified:January 9, 2007 - v1
Checksum:iD64CDD90BABD2956
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000485 Genomic DNA. Translation: ABK85256.1.
RefSeqiYP_894763.1. NC_008600.1.

Genome annotation databases

EnsemblBacteriaiABK85256; ABK85256; BALH_1940.
GeneIDi4545547.
KEGGibtl:BALH_1940.
PATRICi18996367. VBIBacThu63319_2185.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000485 Genomic DNA. Translation: ABK85256.1 .
RefSeqi YP_894763.1. NC_008600.1.

3D structure databases

ProteinModelPortali A0RDG3.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 412694.BALH_1940.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ABK85256 ; ABK85256 ; BALH_1940 .
GeneIDi 4545547.
KEGGi btl:BALH_1940.
PATRICi 18996367. VBIBacThu63319_2185.

Phylogenomic databases

eggNOGi COG0060.
HOGENOMi HOG000246403.
KOi K01870.
OMAi RVEHMVE.
OrthoDBi EOG644ZM1.

Enzyme and pathway databases

BioCyci BTHU412694:GH1W-1948-MONOMER.

Family and domain databases

Gene3Di 1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPi MF_02003. Ile_tRNA_synth_type2.
InterProi IPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023586. Ile-tRNA-ligase_type2.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view ]
Pfami PF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
[Graphical view ]
PRINTSi PR00984. TRNASYNTHILE.
SUPFAMi SSF47323. SSF47323. 2 hits.
SSF50677. SSF50677. 1 hit.
TIGRFAMsi TIGR00392. ileS. 1 hit.
PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Al Hakam.

Entry informationi

Entry nameiSYI_BACAH
AccessioniPrimary (citable) accession number: A0RDG3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: January 9, 2007
Last modified: October 29, 2014
This is version 62 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3