Reviewed, UniProtKB/Swiss-Prot A0RD64 (3HAO_BACAH)

Names and origin

Protein names

Recommended name:
    3-hydroxyanthranilate 3,4-dioxygenase
    EC=1.13.11.6
Alternative name(s):
    3-hydroxyanthranilic acid dioxygenase
      Short name=HAD
    3-hydroxyanthranilate oxygenase
      Short name=3-HAO

Gene names

Ordered Locus Names:

BALH_1838

Organism

Bacillus thuringiensis (strain Al Hakam) [Complete proteome] [HAMAP]

Taxonomic identifier

412694 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Bacillaceae › Bacillus › Bacillus cereus group

Protein attributes

Sequence length	179 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the oxidative ring opening of 3-hydroxyanthranilate to 2-amino-3-carboxymuconate semialdehyde, which spontaneously cyclizes to quinolinate By similarity.
Catalytic activity	3-hydroxyanthranilate + O2 = 2-amino-3-carboxymuconate semialdehyde. HAMAP MF_00825
Cofactor	Binds 2 Fe2+ ions per subunit By similarity.
Sequence similarities	Belongs to the 3-HAO family.

Ontologies

Keywords
Ligand	Iron Metal-binding
Molecular function	Dioxygenase Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	3-hydroxyanthranilate 3,4-dioxygenase activity Inferred from electronic annotation. Source: HAMAP ferrous iron binding Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 179	179	3-hydroxyanthranilate 3,4-dioxygenase HAMAP MF_00825		PRO_0000309575
Sites
	Metal binding	51	1	Iron 1; catalytic By similarity
	Metal binding	57	1	Iron 1; catalytic By similarity
	Metal binding	96	1	Iron 1; catalytic By similarity
	Metal binding	125	1	Iron 2 By similarity
	Metal binding	128	1	Iron 2 By similarity
	Metal binding	162	1	Iron 2 By similarity
	Metal binding	165	1	Iron 2 By similarity
	Binding site	47	1	Dioxygen By similarity
	Binding site	57	1	Substrate By similarity
	Binding site	100	1	Substrate By similarity
	Binding site	110	1	Substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	A0RD64-1 [UniParc]. Last modified January 9, 2007. Version 1. Checksum: 1A5BF15A9B2302AC	FASTA	179	20,985
	10 20 30 40 50 60 MSKTLQSFNL LKWIDENKEL LKPPVNNKVI WQDSEFIAMI LGGPNRRRDF HVDPSDEFFY 70 80 90 100 110 120 QIKGECYVEC ITEEGKREVV TVKEGDVFML PAMVPHSPHR VANTYGLVIE RKRSQGELED 130 140 150 160 170 FVWFCDECNH EMHRVRVQLS DIEKQVKEAI HSFNSNKEIR ACKNCGHIMP EEVGEWKCE

References

[1]

"The complete genome sequence of Bacillus thuringiensis Al Hakam." Challacombe J.F., Altherr M.R., Xie G., Bhotika S.S., Brown N., Bruce D., Campbell C.S., Campbell M.L., Chen J., Chertkov O., Cleland C., Dimitrijevic M., Doggett N.A., Fawcett J.J., Glavina T., Goodwin L.A., Green L.D., Han C.S. , Hill K.K., Hitchcock P., Jackson P.J., Keim P., Kewalramani A.R., Longmire J., Lucas S., Malfatti S., Martinez D., McMurry K., Meincke L.J., Misra M., Moseman B.L., Mundt M., Munk A.C., Okinaka R.T., Parson-Quintana B., Reilly L.P., Richardson P., Robinson D.L., Saunders E., Tapia R., Tesmer J.G., Thayer N., Thompson L.S., Tice H., Ticknor L.O., Wills P.L., Gilna P., Brettin T.S. J. Bacteriol. 189:3680-3681(2007) [PubMed: 17337577] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000485 Genomic DNA. Translation: ABK85157.1.
RefSeq	YP_894664.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	4545488.
GenomeReviews	Gene locus BALH_1838 in contig CP000485_GR.
KEGG	btl:BALH_1838.
NMPDR	fig|412694.5.peg.1824.
Organism-specific databases
CMR	Search...
Phylogenomic databases
OMA	A0RD64. RHSPQRP.
Family and domain databases
HAMAP	MF_00825. [Tree]
InterPro	IPR010329. 3hydroanth_dOase. [Graphical view]
Pfam	PF06052. 3-HAO. 1 hit. [Graphical view]
TIGRFAMs	TIGR03037. anthran_nbaC. 1 hit.
ProtoNet	Search...

Entry information

Entry name

3HAO_BACAH

Accession

Primary (citable) accession number: A0RD64

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 13, 2007
Last sequence update:	January 9, 2007
Last modified:	June 16, 2009
This is version 18 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)