A0RCL3 (ILVD_BACAH) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Dihydroxy-acid dehydratase
Short name=DAD
EC=4.2.1.9

Gene names

Name:	ilvD
Ordered Locus Names:	BALH_1628

Organism

Bacillus thuringiensis (strain Al Hakam) [Complete proteome] [HAMAP]

Taxonomic identifier

412694 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Bacillaceae › Bacillus › Bacillus cereus group

Protein attributes

Sequence length	557 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	2,3-dihydroxy-3-methylbutanoate = 3-methyl-2-oxobutanoate + H2O. HAMAP MF_00012
Cofactor	Binds 1 4Fe-4S cluster Potential.
Pathway	Amino-acid biosynthesis; L-isoleucine biosynthesis; L-isoleucine from 2-oxobutanoate: step 3/4. HAMAP MF_00012 Amino-acid biosynthesis; L-valine biosynthesis; L-valine from pyruvate: step 3/4. HAMAP MF_00012
Sequence similarities	Belongs to the IlvD/Edd family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Branched-chain amino acid biosynthesis
Ligand	4Fe-4S Iron Iron-sulfur Metal-binding
Molecular function	Lyase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	branched chain family amino acid biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	4 iron, 4 sulfur cluster binding Inferred from electronic annotation. Source: UniProtKB-KW dihydroxy-acid dehydratase activity Inferred from electronic annotation. Source: EC metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 557	557	Dihydroxy-acid dehydratase HAMAP MF_00012		PRO_1000000957
Sites
	Metal binding	119	1	Iron-sulfur (4Fe-4S) Potential
	Metal binding	192	1	Iron-sulfur (4Fe-4S) Potential

Sequences

	Sequence		Length	Mass (Da)
	A0RCL3 [UniParc]. Last modified January 9, 2007. Version 1. Checksum: 39AACA4DC2C3C30B	FASTA	557	59,992
	10 20 30 40 50 60 MRSDMIKKGF DKAPHRSLLK ATGLKDEDFD KPFIAICNSF IEIIPGHKHL NEFGKLVKEA 70 80 90 100 110 120 VRAAGMVPFE FNTIGVDDGI AMGHIGMRYS LPSREIIADS VETVVNAHWF DGMICIPNCD 130 140 150 160 170 180 KITPGMMMAA LRINIPTVFV SGGPMAAGKT SKGDVVDLSS VFEGVGAYQS GKISEEELKD 190 200 210 220 230 240 IEDHGCPSCG SCSGMFTANS MNCLCEVLGL ALPGNGSILA IDPRREELIK QAAEKLKILI 250 260 270 280 290 300 ERDIKPRDIV TEEAIDDAFA LDMAMGGSTN TVLHTLALAQ EAGLDYDMNR IDAVSRRVPH 310 320 330 340 350 360 LCKVSPASNW HMEDIDRAGG ISAILKEMSR KEGVLHLDRI TATGQTLREN IAHAEIKDKE 370 380 390 400 410 420 VIHSLENPHS EEGGLRILKG NLAKDGAVIK SGATEVKRFE GPCVIFNSQD EALAGIMLGK 430 440 450 460 470 480 VKKGDVVVIR YEGPRGGPGM PEMLAPTSAI AGMGLGADVA LLTDGRFSGA SRGISVGHIS 490 500 510 520 530 540 PEAAAGGTIA LLEQGDIVCI DVEERLLEVR VSDEELDKRK KEWKRPEPKV KTGWLGRYAQ 550 MVTSANTGAV LKIPNFD

References

[1]

"The complete genome sequence of Bacillus thuringiensis Al Hakam." Challacombe J.F., Altherr M.R., Xie G., Bhotika S.S., Brown N., Bruce D., Campbell C.S., Campbell M.L., Chen J., Chertkov O., Cleland C., Dimitrijevic M., Doggett N.A., Fawcett J.J., Glavina T., Goodwin L.A., Green L.D., Han C.S. , Hill K.K., Hitchcock P., Jackson P.J., Keim P., Kewalramani A.R., Longmire J., Lucas S., Malfatti S., Martinez D., McMurry K., Meincke L.J., Misra M., Moseman B.L., Mundt M., Munk A.C., Okinaka R.T., Parson-Quintana B., Reilly L.P., Richardson P., Robinson D.L., Saunders E., Tapia R., Tesmer J.G., Thayer N., Thompson L.S., Tice H., Ticknor L.O., Wills P.L., Gilna P., Brettin T.S. J. Bacteriol. 189:3680-3681(2007) [PubMed: 17337577] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Al Hakam.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000485 Genomic DNA. Translation: ABK84956.1.
RefSeq	YP_894463.1. NC_008600.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	A0RCL3.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBBACT00000067609; EBBACP00000065846; EBBACG00000067600.
GeneID	4546731.
GenomeReviews	Gene locus BALH_1628 in contig CP000485_GR.
KEGG	btl:BALH_1628.
NMPDR	fig|412694.5.peg.1624.
PATRIC	18995692. VBIBacThu63319_1848.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0129.
GeneTree	EBGT00050000001430.
HOGENOM	HBG671001.
OMA	SQDCRWP.
ProtClustDB	PRK00911.
Enzyme and pathway databases
BioCyc	BTHU412694:BALH_1628-MONOMER.
Family and domain databases
HAMAP	MF_00012. IlvD. [Tree]
InterPro	IPR015928. Aconitase/3IPM_dehydase_swvl. IPR004404. DihydroxyA_deHydtase. IPR000581. DiOHA_6PGluconate_deHydtase. IPR020558. DiOHA_6PGluconate_deHydtase_CS. [Graphical view]
KO	K01687.
PANTHER	PTHR21000. ILVD_EDD_family. 1 hit.
Pfam	PF00920. ILVD_EDD. 1 hit. [Graphical view]
SUPFAM	SSF52016. Aconitase/3IPM_dehydase_swvl. 1 hit.
TIGRFAMs	TIGR00110. IlvD. 1 hit.
PROSITE	PS00886. ILVD_EDD_1. 1 hit. PS00887. ILVD_EDD_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ILVD_BACAH

Accession

Primary (citable) accession number: A0RCL3

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	January 9, 2007
Last modified:	December 14, 2011
This is version 41 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program