A0RBG4 (A0RBG4_BACAH) Unreviewed, UniProtKB/TrEMBL

Names and origin

Protein names

Recommended name:
NADPH-dependent 7-cyano-7-deazaguanine reductase HAMAP MF_00818
EC=1.7.1.13 HAMAP MF_00818

Alternative name(s):
7-cyano-7-carbaguanine reductase HAMAP MF_00818
NADPH-dependent nitrile oxidoreductase HAMAP MF_00818
PreQ(0) reductase HAMAP MF_00818

Gene names

Name:	queF HAMAP MF_00818
Ordered Locus Names:	BALH_1205

Organism

Bacillus thuringiensis (strain Al Hakam) [Complete proteome] [HAMAP]

Taxonomic identifier

412694 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Bacillaceae › Bacillus › Bacillus cereus group

Protein attributes

Sequence length	168 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the NADPH-dependent reduction of 7-cyano-7-deazaguanine (preQ0) to 7-aminomethyl-7-deazaguanine (preQ1) By similarity. HAMAP MF_00818 SAAS SAAS016856
Catalytic activity	7-aminomethyl-7-carbaguanine + 2 NADP+ = 7-cyano-7-carbaguanine + 2 NADPH. HAMAP MF_00818 SAAS SAAS016856
Pathway	tRNA modification; tRNA-queuosine biosynthesis. HAMAP MF_00818 SAAS SAAS016856
Subcellular location	Cytoplasm By similarity HAMAP MF_00818 SAAS SAAS016856.
Sequence similarities	Belongs to the GTP cyclohydrolase I family. QueF type 1 subfamily. HAMAP MF_00818

Ontologies

Keywords
Biological process	Queuosine biosynthesis HAMAP MF_00818 SAAS SAAS016856
Cellular component	Cytoplasm HAMAP MF_00818 SAAS SAAS016856
Ligand	NADP HAMAP MF_00818 SAAS SAAS016856
Molecular function	Hydrolase EMBL ABK84557.1 Oxidoreductase HAMAP MF_00818 SAAS SAAS016856
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	queuosine biosynthetic process Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	hydrolase activity Inferred from electronic annotation. Source: UniProtKB-KW oxidoreductase activity, acting on other nitrogenous compounds as donors, with NAD or NADP as acceptor Inferred from electronic annotation. Source: HAMAP preQ1 synthase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequences

	Sequence		Length	Mass (Da)
	A0RBG4 [UniParc]. Last modified January 9, 2007. Version 1. Checksum: 9C2054A5A8094EC0	FASTA	168	19,935
	10 20 30 40 50 60 MFKMAGRLDE DLKDVTLLGN QNTKYLFEYS PEILEVFDNN HPNRDYFVKF NCPEFTSLCP 70 80 90 100 110 120 KTGQPDFATI YISYIPEQRM VESKSLKLYL FSFRNHGDFH EDCMNVIMND LIKLMDPRYI 130 140 150 160 EVWGKFTPRG GISIDPYCNY GRPGTKYEQM ADYRMMNHDL YPETIDNR

References

[1]

"The complete genome sequence of Bacillus thuringiensis Al Hakam." Challacombe J.F., Altherr M.R., Xie G., Bhotika S.S., Brown N., Bruce D., Campbell C.S., Campbell M.L., Chen J., Chertkov O., Cleland C., Dimitrijevic M., Doggett N.A., Fawcett J.J., Glavina T., Goodwin L.A., Green L.D., Han C.S. , Hill K.K., Hitchcock P., Jackson P.J., Keim P., Kewalramani A.R., Longmire J., Lucas S., Malfatti S., Martinez D., McMurry K., Meincke L.J., Misra M., Moseman B.L., Mundt M., Munk A.C., Okinaka R.T., Parson-Quintana B., Reilly L.P., Richardson P., Robinson D.L., Saunders E., Tapia R., Tesmer J.G., Thayer N., Thompson L.S., Tice H., Ticknor L.O., Wills P.L., Gilna P., Brettin T.S. J. Bacteriol. 189:3680-3681(2007) [PubMed: 17337577] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000485 Genomic DNA. Translation: ABK84557.1.
RefSeq	YP_894064.1. NC_008600.1.
3D structure databases
ProteinModelPortal	A0RBG4.
ModBase	Search...
Protein-protein interaction databases
STRING	A0RBG4.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBBACT00000069225; EBBACP00000067462; EBBACG00000069216.
GeneID	4546197.
GenomeReviews	Gene locus BALH_1205 in contig CP000485_GR.
KEGG	btl:BALH_1205.
PATRIC	18994794. VBIBacThu63319_1400.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0780.
GeneTree	EBGT00050000002144.
HOGENOM	HBG294103.
OMA	CINTILL.
ProtClustDB	PRK13258.
Family and domain databases
HAMAP	MF_00818. QueF_type1. [Tree]
InterPro	IPR016856. CN_OxRdtase_NADPH-dep_QueF. IPR020602. GTP_CycHdrlase_I/CN_OxRdtase. [Graphical view]
KO	K09457.
Pfam	PF01227. GTP_cyclohydroI. 1 hit. [Graphical view]
PIRSF	PIRSF027377. Nitrile_oxidored_QueF. 1 hit.
TIGRFAMs	TIGR03139. QueF-II. 1 hit.
ProtoNet	Search...

Entry information

Entry name

A0RBG4_BACAH

Accession

Primary (citable) accession number: A0RBG4

Entry history

Integrated into UniProtKB/TrEMBL:	January 9, 2007
Last sequence update:	January 9, 2007
Last modified:	December 14, 2011
This is version 43 of the entry and version 1 of the sequence. [Complete history]

Entry status

Unreviewed (UniProtKB/TrEMBL)