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Protein

Hydroxyethylthiazole kinase

Gene

thiM

Organism
Bacillus thuringiensis (strain Al Hakam)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the phosphorylation of the hydroxyl group of 4-methyl-5-beta-hydroxyethylthiazole (THZ).UniRule annotation

Catalytic activityi

ATP + 4-methyl-5-(2-hydroxyethyl)thiazole = ADP + 4-methyl-5-(2-phosphonooxyethyl)thiazole.UniRule annotation

Cofactori

Mg2+UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei45 – 451Substrate; via amide nitrogenUniRule annotation
Binding sitei121 – 1211ATPUniRule annotation
Binding sitei167 – 1671ATPUniRule annotation
Binding sitei194 – 1941Substrate; via amide nitrogenUniRule annotation

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-HAMAP
  2. hydroxyethylthiazole kinase activity Source: UniProtKB-HAMAP
  3. magnesium ion binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. thiamine biosynthetic process Source: UniProtKB-KW
  2. thiamine diphosphate biosynthetic process Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Biological processi

Thiamine biosynthesis

Keywords - Ligandi

ATP-binding, Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciBTHU412694:GH1W-422-MONOMER.
UniPathwayiUPA00060; UER00139.

Names & Taxonomyi

Protein namesi
Recommended name:
Hydroxyethylthiazole kinaseUniRule annotation (EC:2.7.1.50UniRule annotation)
Alternative name(s):
4-methyl-5-beta-hydroxyethylthiazole kinaseUniRule annotation
Short name:
TH kinaseUniRule annotation
Short name:
Thz kinaseUniRule annotation
Gene namesi
Name:thiMUniRule annotation
Ordered Locus Names:BALH_0374
OrganismiBacillus thuringiensis (strain Al Hakam)
Taxonomic identifieri412694 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillusBacillus cereus group
ProteomesiUP000000761 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 268268Hydroxyethylthiazole kinasePRO_0000336546Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi412694.BALH_0374.

Structurei

3D structure databases

ProteinModelPortaliA0R980.
SMRiA0R980. Positions 1-268.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the Thz kinase family.UniRule annotation

Phylogenomic databases

eggNOGiCOG2145.
HOGENOMiHOG000114352.
KOiK00878.
OrthoDBiEOG628F8M.

Family and domain databases

Gene3Di3.40.1190.20. 1 hit.
HAMAPiMF_00228. Thz_kinase.
InterProiIPR000417. Hyethyz_kinase.
IPR029056. Ribokinase-like.
[Graphical view]
PfamiPF02110. HK. 1 hit.
[Graphical view]
PIRSFiPIRSF000513. Thz_kinase. 1 hit.
PRINTSiPR01099. HYETHTZKNASE.
SUPFAMiSSF53613. SSF53613. 1 hit.
TIGRFAMsiTIGR00694. thiM. 1 hit.

Sequencei

Sequence statusi: Complete.

A0R980-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNMKEISKVV DLVRESNPLV HNITNVVVTN FTANGLLALG ASPVMAYAKE
60 70 80 90 100
EVAEMASIAG ALVLNMGTLR PDEVEAMLLA GKSANRNDVP VLFDPVGAGA
110 120 130 140 150
TSYRTEVARH IPAEIELAII RGNAAEIANV INEKWEIKGV DAGAGNGNVV
160 170 180 190 200
SIAKQAADEL NTVAVITGKE DVVTDGERTI VIRNGHSILT KITGTGCLLT
210 220 230 240 250
SVIGAFVAVE KDYVKAAVAA LTFYGVAAEL AAAKTVEKGP GSFQIEFLNQ
260
LANTTSGDIE KYGKIEVI
Length:268
Mass (Da):28,089
Last modified:May 20, 2008 - v2
Checksum:iB532AE73DB99EFEB
GO

Sequence cautioni

The sequence ABK83773.1 differs from that shown. Reason: Erroneous initiation. Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000485 Genomic DNA. Translation: ABK83773.1. Different initiation.
RefSeqiYP_893280.1. NC_008600.1.

Genome annotation databases

EnsemblBacteriaiABK83773; ABK83773; BALH_0374.
GeneIDi4542167.
KEGGibtl:BALH_0374.
PATRICi18992861. VBIBacThu63319_0477.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000485 Genomic DNA. Translation: ABK83773.1. Different initiation.
RefSeqiYP_893280.1. NC_008600.1.

3D structure databases

ProteinModelPortaliA0R980.
SMRiA0R980. Positions 1-268.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi412694.BALH_0374.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABK83773; ABK83773; BALH_0374.
GeneIDi4542167.
KEGGibtl:BALH_0374.
PATRICi18992861. VBIBacThu63319_0477.

Phylogenomic databases

eggNOGiCOG2145.
HOGENOMiHOG000114352.
KOiK00878.
OrthoDBiEOG628F8M.

Enzyme and pathway databases

UniPathwayiUPA00060; UER00139.
BioCyciBTHU412694:GH1W-422-MONOMER.

Family and domain databases

Gene3Di3.40.1190.20. 1 hit.
HAMAPiMF_00228. Thz_kinase.
InterProiIPR000417. Hyethyz_kinase.
IPR029056. Ribokinase-like.
[Graphical view]
PfamiPF02110. HK. 1 hit.
[Graphical view]
PIRSFiPIRSF000513. Thz_kinase. 1 hit.
PRINTSiPR01099. HYETHTZKNASE.
SUPFAMiSSF53613. SSF53613. 1 hit.
TIGRFAMsiTIGR00694. thiM. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Al Hakam.

Entry informationi

Entry nameiTHIM_BACAH
AccessioniPrimary (citable) accession number: A0R980
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 20, 2008
Last sequence update: May 20, 2008
Last modified: January 7, 2015
This is version 58 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.