A0R8Z7 (A0R8Z7_BACAH) Unreviewed, UniProtKB/TrEMBL

Names and origin

Protein names

Recommended name:
Amidophosphoribosyltransferase PIRNR PIRNR000485
Short name=ATase PIRNR PIRNR000485
EC=2.4.2.14 PIRNR PIRNR000485

Alternative name(s):
Glutamine phosphoribosylpyrophosphate amidotransferase PIRNR PIRNR000485

Gene names

Name:	purF EMBL ABK83690.1
Ordered Locus Names:	BALH_0289 EMBL ABK83690.1

Organism

Bacillus thuringiensis (strain Al Hakam) [Complete proteome] [HAMAP] EMBL ABK83690.1

Taxonomic identifier

412694 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Firmicutes › Bacilli › Bacillales › Bacillaceae › Bacillus › Bacillus cereus group › Bacillus thuringiensis

Protein attributes

Sequence length	477 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	5-phospho-beta-D-ribosylamine + diphosphate + L-glutamate = L-glutamine + 5-phospho-alpha-D-ribose 1-diphosphate + H2O. PIRNR PIRNR000485
Cofactor	Binds 1 magnesium ion per subunit By similarity. PIRNR PIRNR000485
Pathway	Purine metabolism; IMP biosynthesis via de novo pathway; N(1)-(5-phospho-D-ribosyl)glycinamide from 5-phospho-alpha-D-ribose 1-diphosphate: step 1/2. PIRNR PIRNR000485
Sequence similarities	In the C-terminal section; belongs to the purine/pyrimidine phosphoribosyltransferase family. PIRNR PIRNR000485

Ontologies

Keywords
Biological process	Purine biosynthesis PIRNR PIRNR000485
Ligand	Magnesium PIRNR PIRNR000485 Metal-binding PIRNR PIRNR000485
Molecular function	Glycosyltransferase PIRNR PIRNR000485 EMBL ABK83690.1 Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	'de novo' IMP biosynthetic process Inferred from electronic annotation. Source: UniProtKB-UniPathway nucleoside metabolic process Inferred from electronic annotation. Source: InterPro purine nucleobase biosynthetic process Inferred from electronic annotation. Source: InterPro
Molecular_function	amidophosphoribosyltransferase activity Inferred from electronic annotation. Source: EC metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Sites
	Active site	18	1	For GATase activity By similarity PIRSR PIRSR000485-1

Sequences

	Sequence		Length	Mass (Da)
	A0R8Z7 [UniParc]. Last modified January 9, 2007. Version 1. Checksum: E92AA1B798521BB8	FASTA	477	52,089
	10 20 30 40 50 60 MERGNPMLAE IKGLNEECGV FGIWGHENAA QVSYYGLHSL QHRGQEGAGI VVNNGEKIVG 70 80 90 100 110 120 HKGLGLISEV FSRGELEGLN GKSAIGHVRY ATAGGSEVAN VQPLLFRFSD HSMALAHNGN 130 140 150 160 170 180 LINAKMLRRE LEAEGSIFQT SSDTEVLLHL IKRSTKDSLI ESVKEALNKV KGAFAYLLLT 190 200 210 220 230 240 GNEMIVALDP NGFRPLSIGK MGDAYVVASE TCAFDVVGAT YIRDVEPGEL LIINDEGIHV 250 260 270 280 290 300 DRFTNEVDHA ICSMEYIYFA RPDSNIAGIN VHAARKNMGK RLAAEAPIEA DVVTGVPDSS 310 320 330 340 350 360 ISAAIGYAEA TGIPYELGLI KNRYVGRTFI QPSQELREQG VKMKLSAVRG VVEGKRVVMI 370 380 390 400 410 420 DDSIVRGTTS KRIVRMLREA GATEVHVRIA SPPLKYPCFY GIDIQTRKEL IAANHTVEEI 430 440 450 460 470 REMIGADSLT FLSEDGLVDA IGRPYEGKYG GLCMAYFNGD YPTALYDYEQ ELLESMK

References

[1]

"The complete genome sequence of Bacillus thuringiensis Al Hakam." Challacombe J.F., Altherr M.R., Xie G., Bhotika S.S., Brown N., Bruce D., Campbell C.S., Campbell M.L., Chen J., Chertkov O., Cleland C., Dimitrijevic M., Doggett N.A., Fawcett J.J., Glavina T., Goodwin L.A., Green L.D., Han C.S. , Hill K.K., Hitchcock P., Jackson P.J., Keim P., Kewalramani A.R., Longmire J., Lucas S., Malfatti S., Martinez D., McMurry K., Meincke L.J., Misra M., Moseman B.L., Mundt M., Munk A.C., Okinaka R.T., Parson-Quintana B., Reilly L.P., Richardson P., Robinson D.L., Saunders E., Tapia R., Tesmer J.G., Thayer N., Thompson L.S., Tice H., Ticknor L.O., Wills P.L., Gilna P., Brettin T.S. J. Bacteriol. 189:3680-3681(2007) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Al Hakam EMBL ABK83690.1.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000485 Genomic DNA. Translation: ABK83690.1.
RefSeq	YP_893197.1. NC_008600.1.
3D structure databases
ProteinModelPortal	A0R8Z7.
SMR	A0R8Z7. Positions 18-466.
ModBase	Search...
Protein-protein interaction databases
STRING	412694.BALH_0289.
Protein family/group databases
MEROPS	C44.001.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	ABK83690; ABK83690; BALH_0289.
GeneID	4544203.
KEGG	btl:BALH_0289.
PATRIC	18992677. VBIBacThu63319_0385.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0034.
HOGENOM	HOG000033688.
KO	K00764.
OMA	GIPFELG.
ProtClustDB	PRK06781.
Enzyme and pathway databases
BioCyc	BTHU412694:GH1W-228-MONOMER.
UniPathway	UPA00074; UER00124.
Family and domain databases
InterPro	IPR005854. Amd_phspho_trans. IPR017932. GATase_2_dom. IPR000583. GATase_dom. IPR000836. PRibTrfase_dom. [Graphical view]
Pfam	PF00310. GATase_2. 1 hit. PF00156. Pribosyltran. 1 hit. [Graphical view]
PIRSF	PIRSF000485. Amd_phspho_trans. 1 hit.
TIGRFAMs	TIGR01134. purF. 1 hit.
PROSITE	PS51278. GATASE_TYPE_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

A0R8Z7_BACAH

Accession

Primary (citable) accession number: A0R8Z7

Entry history

Integrated into UniProtKB/TrEMBL:	January 9, 2007
Last sequence update:	January 9, 2007
Last modified:	May 1, 2013
This is version 46 of the entry and version 1 of the sequence. [Complete history]

Entry status

Unreviewed (UniProtKB/TrEMBL)