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Protein

Amidophosphoribosyltransferase

Gene

purF

Organism
Bacillus thuringiensis (strain Al Hakam)
Status
Unreviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the formation of phosphoribosylamine from phosphoribosylpyrophosphate (PRPP) and glutamine.UniRule annotation

Caution

Lacks conserved residue(s) required for the propagation of feature annotation.UniRule annotation

Catalytic activityi

5-phospho-beta-D-ribosylamine + diphosphate + L-glutamate = L-glutamine + 5-phospho-alpha-D-ribose 1-diphosphate + H2O.UniRule annotation

Cofactori

Mg2+UniRule annotationNote: Binds 1 Mg2+ ion per subunit.UniRule annotation

Pathwayi: IMP biosynthesis via de novo pathway

This protein is involved in step 1 of the subpathway that synthesizes N(1)-(5-phospho-D-ribosyl)glycinamide from 5-phospho-alpha-D-ribose 1-diphosphate.UniRule annotation
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. Amidophosphoribosyltransferase (purF)
  2. Phosphoribosylamine--glycine ligase (purD)
This subpathway is part of the pathway IMP biosynthesis via de novo pathway, which is itself part of Purine metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes N(1)-(5-phospho-D-ribosyl)glycinamide from 5-phospho-alpha-D-ribose 1-diphosphate, the pathway IMP biosynthesis via de novo pathway and in Purine metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei18For GATase activityUniRule annotation1
Active sitei18NucleophileUniRule annotation1
Metal bindingi299MagnesiumUniRule annotation1
Metal bindingi361MagnesiumUniRule annotation1
Metal bindingi362MagnesiumUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionGlycosyltransferaseUniRule annotationImported, Transferase
Biological processPurine biosynthesisUniRule annotation
LigandMagnesiumUniRule annotation, Metal-bindingUniRule annotation

Enzyme and pathway databases

UniPathwayiUPA00074; UER00124

Protein family/group databases

MEROPSiC44.001

Names & Taxonomyi

Protein namesi
Recommended name:
AmidophosphoribosyltransferaseUniRule annotation (EC:2.4.2.14UniRule annotation)
Short name:
ATaseUniRule annotation
Alternative name(s):
Glutamine phosphoribosylpyrophosphate amidotransferaseUniRule annotation
Short name:
GPATaseUniRule annotation
Gene namesi
Name:purFUniRule annotationImported
Ordered Locus Names:BALH_0289Imported
OrganismiBacillus thuringiensis (strain Al Hakam)Imported
Taxonomic identifieri412694 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillusBacillus cereus group
Proteomesi
  • UP000000761 Componenti: Chromosome

Structurei

3D structure databases

ProteinModelPortaliA0R8Z7
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini18 – 236Glutamine amidotransferase type-2InterPro annotationAdd BLAST219

Sequence similaritiesi

In the C-terminal section; belongs to the purine/pyrimidine phosphoribosyltransferase family.UniRule annotation

Keywords - Domaini

Glutamine amidotransferaseUniRule annotation

Phylogenomic databases

HOGENOMiHOG000033688
KOiK00764
OMAiFRPLCLG

Family and domain databases

CDDicd00715 GPATase_N, 1 hit
cd06223 PRTases_typeI, 1 hit
Gene3Di3.60.20.10, 3 hits
HAMAPiMF_01931 PurF, 1 hit
InterProiView protein in InterPro
IPR017932 GATase_2_dom
IPR029055 Ntn_hydrolases_N
IPR000836 PRibTrfase_dom
IPR029057 PRTase-like
IPR005854 PurF
IPR035584 PurF_N
PfamiView protein in Pfam
PF13537 GATase_7, 1 hit
PF00156 Pribosyltran, 1 hit
PIRSFiPIRSF000485 Amd_phspho_trans, 1 hit
SUPFAMiSSF53271 SSF53271, 1 hit
SSF56235 SSF56235, 1 hit
TIGRFAMsiTIGR01134 purF, 1 hit
PROSITEiView protein in PROSITE
PS51278 GATASE_TYPE_2, 1 hit

Sequencei

Sequence statusi: Complete.

A0R8Z7-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MERGNPMLAE IKGLNEECGV FGIWGHENAA QVSYYGLHSL QHRGQEGAGI
60 70 80 90 100
VVNNGEKIVG HKGLGLISEV FSRGELEGLN GKSAIGHVRY ATAGGSEVAN
110 120 130 140 150
VQPLLFRFSD HSMALAHNGN LINAKMLRRE LEAEGSIFQT SSDTEVLLHL
160 170 180 190 200
IKRSTKDSLI ESVKEALNKV KGAFAYLLLT GNEMIVALDP NGFRPLSIGK
210 220 230 240 250
MGDAYVVASE TCAFDVVGAT YIRDVEPGEL LIINDEGIHV DRFTNEVDHA
260 270 280 290 300
ICSMEYIYFA RPDSNIAGIN VHAARKNMGK RLAAEAPIEA DVVTGVPDSS
310 320 330 340 350
ISAAIGYAEA TGIPYELGLI KNRYVGRTFI QPSQELREQG VKMKLSAVRG
360 370 380 390 400
VVEGKRVVMI DDSIVRGTTS KRIVRMLREA GATEVHVRIA SPPLKYPCFY
410 420 430 440 450
GIDIQTRKEL IAANHTVEEI REMIGADSLT FLSEDGLVDA IGRPYEGKYG
460 470
GLCMAYFNGD YPTALYDYEQ ELLESMK
Length:477
Mass (Da):52,089
Last modified:January 9, 2007 - v1
Checksum:iE92AA1B798521BB8
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000485 Genomic DNA Translation: ABK83690.1

Genome annotation databases

EnsemblBacteriaiABK83690; ABK83690; BALH_0289
KEGGibtl:BALH_0289

Similar proteinsi

Entry informationi

Entry nameiA0R8Z7_BACAH
AccessioniPrimary (citable) accession number: A0R8Z7
Entry historyiIntegrated into UniProtKB/TrEMBL: January 9, 2007
Last sequence update: January 9, 2007
Last modified: March 28, 2018
This is version 85 of the entry and version 1 of the sequence. See complete history.
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

Complete proteomeImported

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