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Protein

Amidophosphoribosyltransferase

Gene

purF

Organism
Bacillus thuringiensis (strain Al Hakam)
Status
Unreviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the formation of phosphoribosylamine from phosphoribosylpyrophosphate (PRPP) and glutamine.UniRule annotation

Catalytic activityi

5-phospho-beta-D-ribosylamine + diphosphate + L-glutamate = L-glutamine + 5-phospho-alpha-D-ribose 1-diphosphate + H2O.UniRule annotation

Cofactori

Mg2+UniRule annotationNote: Binds 1 Mg2+ ion per subunit.UniRule annotation

Pathway:iIMP biosynthesis via de novo pathway

This protein is involved in step 1 of the subpathway that synthesizes N(1)-(5-phospho-D-ribosyl)glycinamide from 5-phospho-alpha-D-ribose 1-diphosphate.UniRule annotation
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. Amidophosphoribosyltransferase (purF)
  2. Phosphoribosylamine--glycine ligase (purD)
This subpathway is part of the pathway IMP biosynthesis via de novo pathway, which is itself part of Purine metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes N(1)-(5-phospho-D-ribosyl)glycinamide from 5-phospho-alpha-D-ribose 1-diphosphate, the pathway IMP biosynthesis via de novo pathway and in Purine metabolism.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei18 – 181For GATase activityUniRule annotation
Active sitei18 – 181NucleophileUniRule annotation
Metal bindingi299 – 2991MagnesiumUniRule annotation
Metal bindingi361 – 3611MagnesiumUniRule annotation
Metal bindingi362 – 3621MagnesiumUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

GlycosyltransferaseUniRule annotation, Transferase

Keywords - Biological processi

Purine biosynthesisUniRule annotation

Keywords - Ligandi

MagnesiumUniRule annotation, Metal-bindingUniRule annotation

Enzyme and pathway databases

BioCyciBTHU412694:GH1W-339-MONOMER.
UniPathwayiUPA00074; UER00124.

Protein family/group databases

MEROPSiC44.A01.

Names & Taxonomyi

Protein namesi
Recommended name:
AmidophosphoribosyltransferaseUniRule annotation (EC:2.4.2.14UniRule annotation)
Short name:
ATaseUniRule annotation
Alternative name(s):
Glutamine phosphoribosylpyrophosphate amidotransferaseUniRule annotation
Gene namesi
Name:purFUniRule annotationImported
Ordered Locus Names:BALH_0289Imported
OrganismiBacillus thuringiensis (strain Al Hakam)Imported
Taxonomic identifieri412694 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillusBacillus cereus group
ProteomesiUP000000761 Componenti: Chromosome

Structurei

3D structure databases

ProteinModelPortaliA0R8Z7.
SMRiA0R8Z7. Positions 18-466.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini18 – 236219Glutamine amidotransferase type-2UniRule annotationAdd
BLAST

Sequence similaritiesi

Contains 1 glutamine amidotransferase type-2 domain.UniRule annotation
In the C-terminal section; belongs to the purine/pyrimidine phosphoribosyltransferase family.UniRule annotation

Keywords - Domaini

Glutamine amidotransferaseUniRule annotation

Phylogenomic databases

eggNOGiCOG0034.
HOGENOMiHOG000033688.
KOiK00764.
OMAiIRHFGVK.
OrthoDBiEOG6KT2Q1.

Family and domain databases

Gene3Di3.40.50.2020. 1 hit.
3.60.20.10. 1 hit.
HAMAPiMF_01931. PurF.
InterProiIPR017932. GATase_2_dom.
IPR029055. Ntn_hydrolases_N.
IPR000836. PRibTrfase_dom.
IPR029057. PRTase-like.
IPR005854. PurF.
[Graphical view]
PfamiPF00156. Pribosyltran. 1 hit.
[Graphical view]
PIRSFiPIRSF000485. Amd_phspho_trans. 1 hit.
SUPFAMiSSF53271. SSF53271. 1 hit.
SSF56235. SSF56235. 1 hit.
TIGRFAMsiTIGR01134. purF. 1 hit.
PROSITEiPS51278. GATASE_TYPE_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

A0R8Z7-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MERGNPMLAE IKGLNEECGV FGIWGHENAA QVSYYGLHSL QHRGQEGAGI
60 70 80 90 100
VVNNGEKIVG HKGLGLISEV FSRGELEGLN GKSAIGHVRY ATAGGSEVAN
110 120 130 140 150
VQPLLFRFSD HSMALAHNGN LINAKMLRRE LEAEGSIFQT SSDTEVLLHL
160 170 180 190 200
IKRSTKDSLI ESVKEALNKV KGAFAYLLLT GNEMIVALDP NGFRPLSIGK
210 220 230 240 250
MGDAYVVASE TCAFDVVGAT YIRDVEPGEL LIINDEGIHV DRFTNEVDHA
260 270 280 290 300
ICSMEYIYFA RPDSNIAGIN VHAARKNMGK RLAAEAPIEA DVVTGVPDSS
310 320 330 340 350
ISAAIGYAEA TGIPYELGLI KNRYVGRTFI QPSQELREQG VKMKLSAVRG
360 370 380 390 400
VVEGKRVVMI DDSIVRGTTS KRIVRMLREA GATEVHVRIA SPPLKYPCFY
410 420 430 440 450
GIDIQTRKEL IAANHTVEEI REMIGADSLT FLSEDGLVDA IGRPYEGKYG
460 470
GLCMAYFNGD YPTALYDYEQ ELLESMK
Length:477
Mass (Da):52,089
Last modified:January 9, 2007 - v1
Checksum:iE92AA1B798521BB8
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000485 Genomic DNA. Translation: ABK83690.1.

Genome annotation databases

EnsemblBacteriaiABK83690; ABK83690; BALH_0289.
KEGGibtl:BALH_0289.
PATRICi18992677. VBIBacThu63319_0385.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000485 Genomic DNA. Translation: ABK83690.1.

3D structure databases

ProteinModelPortaliA0R8Z7.
SMRiA0R8Z7. Positions 18-466.
ModBaseiSearch...
MobiDBiSearch...

Protein family/group databases

MEROPSiC44.A01.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABK83690; ABK83690; BALH_0289.
KEGGibtl:BALH_0289.
PATRICi18992677. VBIBacThu63319_0385.

Phylogenomic databases

eggNOGiCOG0034.
HOGENOMiHOG000033688.
KOiK00764.
OMAiIRHFGVK.
OrthoDBiEOG6KT2Q1.

Enzyme and pathway databases

UniPathwayiUPA00074; UER00124.
BioCyciBTHU412694:GH1W-339-MONOMER.

Family and domain databases

Gene3Di3.40.50.2020. 1 hit.
3.60.20.10. 1 hit.
HAMAPiMF_01931. PurF.
InterProiIPR017932. GATase_2_dom.
IPR029055. Ntn_hydrolases_N.
IPR000836. PRibTrfase_dom.
IPR029057. PRTase-like.
IPR005854. PurF.
[Graphical view]
PfamiPF00156. Pribosyltran. 1 hit.
[Graphical view]
PIRSFiPIRSF000485. Amd_phspho_trans. 1 hit.
SUPFAMiSSF53271. SSF53271. 1 hit.
SSF56235. SSF56235. 1 hit.
TIGRFAMsiTIGR01134. purF. 1 hit.
PROSITEiPS51278. GATASE_TYPE_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Al HakamImported.

Entry informationi

Entry nameiA0R8Z7_BACAH
AccessioniPrimary (citable) accession number: A0R8Z7
Entry historyi
Integrated into UniProtKB/TrEMBL: January 9, 2007
Last sequence update: January 9, 2007
Last modified: July 22, 2015
This is version 64 of the entry and version 1 of the sequence. [Complete history]
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

Complete proteomeImported

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.