A0R7K1 (DNAA_MYCS2) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Chromosomal replication initiator protein DnaA

Gene names

Name:	dnaA
Ordered Locus Names:	MSMEG_6947

Organism

Mycobacterium smegmatis (strain ATCC 700084 / mc(2)155)

Taxonomic identifier

246196 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Corynebacterineae › Mycobacteriaceae › Mycobacterium

Protein attributes

Sequence length	504 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Plays an important role in the initiation and regulation of chromosomal replication. Binds to the origin of replication; it binds specifically double-stranded DNA at a 9 bp consensus (dnaA box): 5'-TTATC[CA]A[CA]A-3'. DnaA binds to ATP and to acidic phospholipids By similarity. HAMAP MF_00377
Subcellular location	Cytoplasm By similarity HAMAP MF_00377.
Sequence similarities	Belongs to the DnaA family.
Sequence caution	The sequence AAC43711.1 differs from that shown. Reason: Frameshift at several positions.

Ontologies

Keywords
Biological process	DNA replication
Cellular component	Cytoplasm
Ligand	ATP-binding DNA-binding Nucleotide-binding
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	DNA-dependent DNA replication initiation Inferred from electronic annotation. Source: InterPro regulation of DNA replication Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW DNA replication origin binding Inferred from electronic annotation. Source: InterPro nucleoside-triphosphatase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 504	504	Chromosomal replication initiator protein DnaA HAMAP MF_00377		PRO_0000293597
Regions
	Nucleotide binding	205 – 212	8	ATP Potential
Experimental info
	Sequence conflict	218	1	A → H in AAC43711. Ref.1
	Sequence conflict	234	1	V → A in AAC43711. Ref.1
	Sequence conflict	315	1	L → F in AAC43711. Ref.1
	Sequence conflict	447	1	L → M in AAC43711. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	A0R7K1 [UniParc]. Last modified January 9, 2007. Version 1. Checksum: C7609F2A6D4030CB	FASTA	504	56,664
	10 20 30 40 50 60 MTADPDPPFV AVWNSVVAEL NGDVNGDRQG DPSLPVLTPQ QRAWLKLVKP LVIAEGFALL 70 80 90 100 110 120 SVPTPFVQNE IERHLREPIV TALSRKLGQR VELGVRIATP TDEPEDAPDS FADSPAPASV 130 140 150 160 170 180 PAGPADADEI DDDRDARVNA QESWPKYFSR PEPDTSSDDS NAVNLNRRYT FDTFVIGASN 190 200 210 220 230 240 RFAHAATLAI AEAPARAYNP LFIWGESGLG KTHLLHAAGN YAQRLFPGMR VKYVSTEEFT 250 260 270 280 290 300 NDFINSLRDD RKASFKRSYR DIDILLVDDI QFIEGKEGIQ EEFFHTFNTL HNSNKQIVIS 310 320 330 340 350 360 SDRPPKQLAT LEDRLRTRFE WGLITDVQPP ELETRIAILR KKAQMDRLDV PDDVLELIAS 370 380 390 400 410 420 SIERNIRELE GALIRVTAFA SLNKTRIDRS LAEVVLRDLI ADATTMQIST AAIMAVTAEY 430 440 450 460 470 480 FETTVEELRG PGKTRALAQS RQIAMYLCRE LTDLSLPKIG QAFGRDHTTV MYAEKKIRGE 490 500 MAERREVFDH VKELTTRIRQ RAKR

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Mycobacterium smegmatis dnaA region and autonomous replication activity." Rajagopalan M., Qin M.H., Nash D.R., Madiraju M.V.V.S. J. Bacteriol. 177:6527-6535(1995) [PubMed: 7592430] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	Fleischmann R.D., Dodson R.J., Haft D.H., Merkel J.S., Nelson W.C., Fraser C.M. Submitted (OCT-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 700084 / mc(2)155.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U17833 Genomic DNA. Translation: AAC43711.1. Frameshift. CP000480 Genomic DNA. Translation: ABK76223.1.
RefSeq	YP_891139.1. NC_008596.1.
3D structure databases
ProteinModelPortal	A0R7K1.
ModBase	Search...
Protein-protein interaction databases
STRING	A0R7K1.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBMYCT00000042681; EBMYCP00000041018; EBMYCG00000042676.
GeneID	4531721.
GenomeReviews	Gene locus MSMEG_6947 in contig CP000480_GR.
KEGG	msm:MSMEG_6947.
NMPDR	fig|246196.1.peg.6901.
PATRIC	18086094. VBIMycSme59918_6759.
TIGR	MSMEG_6947.
Phylogenomic databases
eggNOG	COG0593.
GeneTree	EBGT00050000017320.
HOGENOM	HBG695666.
OMA	DEVAFLI.
PhylomeDB	A0R7K1.
ProtClustDB	PRK00149.
Enzyme and pathway databases
BioCyc	MSME246196:MSMEG_6947-MONOMER.
Family and domain databases
HAMAP	MF_00377. DnaA_bact. [Tree]
InterPro	IPR003593. ATPase_AAA+_core. IPR001957. Chromosome_initiator_DnaA. IPR020591. Chromosome_initiator_DnaA-like. IPR018312. Chromosome_initiator_DnaA_CS. IPR013159. DnaA_C. IPR013317. DnaA_Hda. IPR010921. Trp_repressor/repl_initiator. [Graphical view]
Gene3D	G3DSA:1.10.1750.10. DnaA_C. 1 hit.
KO	K02313.
Pfam	PF00308. Bac_DnaA. 1 hit. PF08299. Bac_DnaA_C. 1 hit. [Graphical view]
PRINTS	PR00051. DNAA.
SMART	SM00382. AAA. 1 hit. SM00760. Bac_DnaA_C. 1 hit. [Graphical view]
SUPFAM	SSF48295. Trp_repress_rep. 1 hit.
TIGRFAMs	TIGR00362. DnaA. 1 hit.
PROSITE	PS01008. DNAA. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

DNAA_MYCS2

Accession

Primary (citable) accession number: A0R7K1
Secondary accession number(s): P49992, Q59558

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 10, 2007
Last sequence update:	January 9, 2007
Last modified:	December 14, 2011
This is version 36 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program