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Protein

Chromosomal replication initiator protein DnaA

Gene

dnaA

Organism
Mycobacterium smegmatis (strain ATCC 700084 / mc(2)155)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Experimental evidence at transcript leveli

Functioni

Plays an important role in the initiation and regulation of chromosomal replication. Binds to the origin of replication; it binds specifically double-stranded DNA at a 9 bp consensus (dnaA box): 5'-TTATC[CA]A[CA]A-3'. DnaA binds to ATP and to acidic phospholipids (By similarity).By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi205 – 2128ATPSequence analysis

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

DNA replication

Keywords - Ligandi

ATP-binding, DNA-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciMSME246196:GJ4Y-6938-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Chromosomal replication initiator protein DnaA
Gene namesi
Name:dnaA
Ordered Locus Names:MSMEG_6947, MSMEI_0001
OrganismiMycobacterium smegmatis (strain ATCC 700084 / mc(2)155)
Taxonomic identifieri246196 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaCorynebacterialesMycobacteriaceaeMycobacterium
Proteomesi
  • UP000006158 Componenti: Chromosome
  • UP000000757 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 504504Chromosomal replication initiator protein DnaAPRO_0000293597Add
BLAST

Expressioni

Inductioni

Expression depends on the two-component regulatory system MtrA/MtrB.1 Publication

Interactioni

Protein-protein interaction databases

STRINGi246196.MSMEG_6947.

Structurei

3D structure databases

ProteinModelPortaliA0R7K1.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the DnaA family.Curated

Phylogenomic databases

eggNOGiENOG4105CI4. Bacteria.
COG0593. LUCA.
HOGENOMiHOG000235658.
KOiK02313.
OMAiSHACDKI.
OrthoDBiEOG689HR1.

Family and domain databases

Gene3Di1.10.1750.10. 1 hit.
3.40.50.300. 1 hit.
HAMAPiMF_00377. DnaA_bact.
InterProiIPR003593. AAA+_ATPase.
IPR001957. Chromosome_initiator_DnaA.
IPR020591. Chromosome_initiator_DnaA-like.
IPR018312. Chromosome_initiator_DnaA_CS.
IPR013317. DnaA.
IPR013159. DnaA_C.
IPR027417. P-loop_NTPase.
IPR010921. Trp_repressor/repl_initiator.
[Graphical view]
PfamiPF00308. Bac_DnaA. 1 hit.
PF08299. Bac_DnaA_C. 1 hit.
[Graphical view]
PRINTSiPR00051. DNAA.
SMARTiSM00382. AAA. 1 hit.
SM00760. Bac_DnaA_C. 1 hit.
[Graphical view]
SUPFAMiSSF48295. SSF48295. 1 hit.
SSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR00362. DnaA. 1 hit.
PROSITEiPS01008. DNAA. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

A0R7K1-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTADPDPPFV AVWNSVVAEL NGDVNGDRQG DPSLPVLTPQ QRAWLKLVKP
60 70 80 90 100
LVIAEGFALL SVPTPFVQNE IERHLREPIV TALSRKLGQR VELGVRIATP
110 120 130 140 150
TDEPEDAPDS FADSPAPASV PAGPADADEI DDDRDARVNA QESWPKYFSR
160 170 180 190 200
PEPDTSSDDS NAVNLNRRYT FDTFVIGASN RFAHAATLAI AEAPARAYNP
210 220 230 240 250
LFIWGESGLG KTHLLHAAGN YAQRLFPGMR VKYVSTEEFT NDFINSLRDD
260 270 280 290 300
RKASFKRSYR DIDILLVDDI QFIEGKEGIQ EEFFHTFNTL HNSNKQIVIS
310 320 330 340 350
SDRPPKQLAT LEDRLRTRFE WGLITDVQPP ELETRIAILR KKAQMDRLDV
360 370 380 390 400
PDDVLELIAS SIERNIRELE GALIRVTAFA SLNKTRIDRS LAEVVLRDLI
410 420 430 440 450
ADATTMQIST AAIMAVTAEY FETTVEELRG PGKTRALAQS RQIAMYLCRE
460 470 480 490 500
LTDLSLPKIG QAFGRDHTTV MYAEKKIRGE MAERREVFDH VKELTTRIRQ

RAKR
Length:504
Mass (Da):56,664
Last modified:January 9, 2007 - v1
Checksum:iC7609F2A6D4030CB
GO

Sequence cautioni

The sequence AAC43711.1 differs from that shown. Reason: Frameshift at several positions. Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti218 – 2181A → H in AAC43711 (PubMed:7592430).Curated
Sequence conflicti234 – 2341V → A in AAC43711 (PubMed:7592430).Curated
Sequence conflicti315 – 3151L → F in AAC43711 (PubMed:7592430).Curated
Sequence conflicti447 – 4471L → M in AAC43711 (PubMed:7592430).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U17833 Genomic DNA. Translation: AAC43711.1. Frameshift.
CP000480 Genomic DNA. Translation: ABK76223.1.
CP001663 Genomic DNA. Translation: AFP36486.1.
RefSeqiWP_003898364.1. NZ_CP009494.1.
YP_891139.1. NC_008596.1.

Genome annotation databases

EnsemblBacteriaiABK76223; ABK76223; MSMEG_6947.
AFP36486; AFP36486; MSMEI_0001.
GeneIDi4531721.
KEGGimsb:LJ00_34285.
msg:MSMEI_0001.
msm:MSMEG_6947.
PATRICi18086094. VBIMycSme59918_6759.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U17833 Genomic DNA. Translation: AAC43711.1. Frameshift.
CP000480 Genomic DNA. Translation: ABK76223.1.
CP001663 Genomic DNA. Translation: AFP36486.1.
RefSeqiWP_003898364.1. NZ_CP009494.1.
YP_891139.1. NC_008596.1.

3D structure databases

ProteinModelPortaliA0R7K1.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi246196.MSMEG_6947.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABK76223; ABK76223; MSMEG_6947.
AFP36486; AFP36486; MSMEI_0001.
GeneIDi4531721.
KEGGimsb:LJ00_34285.
msg:MSMEI_0001.
msm:MSMEG_6947.
PATRICi18086094. VBIMycSme59918_6759.

Phylogenomic databases

eggNOGiENOG4105CI4. Bacteria.
COG0593. LUCA.
HOGENOMiHOG000235658.
KOiK02313.
OMAiSHACDKI.
OrthoDBiEOG689HR1.

Enzyme and pathway databases

BioCyciMSME246196:GJ4Y-6938-MONOMER.

Family and domain databases

Gene3Di1.10.1750.10. 1 hit.
3.40.50.300. 1 hit.
HAMAPiMF_00377. DnaA_bact.
InterProiIPR003593. AAA+_ATPase.
IPR001957. Chromosome_initiator_DnaA.
IPR020591. Chromosome_initiator_DnaA-like.
IPR018312. Chromosome_initiator_DnaA_CS.
IPR013317. DnaA.
IPR013159. DnaA_C.
IPR027417. P-loop_NTPase.
IPR010921. Trp_repressor/repl_initiator.
[Graphical view]
PfamiPF00308. Bac_DnaA. 1 hit.
PF08299. Bac_DnaA_C. 1 hit.
[Graphical view]
PRINTSiPR00051. DNAA.
SMARTiSM00382. AAA. 1 hit.
SM00760. Bac_DnaA_C. 1 hit.
[Graphical view]
SUPFAMiSSF48295. SSF48295. 1 hit.
SSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR00362. DnaA. 1 hit.
PROSITEiPS01008. DNAA. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Mycobacterium smegmatis dnaA region and autonomous replication activity."
    Rajagopalan M., Qin M.H., Nash D.R., Madiraju M.V.V.S.
    J. Bacteriol. 177:6527-6535(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. Fleischmann R.D., Dodson R.J., Haft D.H., Merkel J.S., Nelson W.C., Fraser C.M.
    Submitted (OCT-2006) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 700084 / mc(2)155.
  3. "Interrupted coding sequences in Mycobacterium smegmatis: authentic mutations or sequencing errors?"
    Deshayes C., Perrodou E., Gallien S., Euphrasie D., Schaeffer C., Van-Dorsselaer A., Poch O., Lecompte O., Reyrat J.-M.
    Genome Biol. 8:R20.1-R20.9(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 700084 / mc(2)155.
  4. "Ortho-proteogenomics: multiple proteomes investigation through orthology and a new MS-based protocol."
    Gallien S., Perrodou E., Carapito C., Deshayes C., Reyrat J.-M., Van Dorsselaer A., Poch O., Schaeffer C., Lecompte O.
    Genome Res. 19:128-135(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 700084 / mc(2)155.
  5. "Septal localization of the Mycobacterium tuberculosis MtrB sensor kinase promotes MtrA regulon expression."
    Plocinska R., Purushotham G., Sarva K., Vadrevu I.S., Pandeeti E.V., Arora N., Plocinski P., Madiraju M.V., Rajagopalan M.
    J. Biol. Chem. 287:23887-23899(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: INDUCTION.
    Strain: ATCC 700084 / mc(2)155.

Entry informationi

Entry nameiDNAA_MYCS2
AccessioniPrimary (citable) accession number: A0R7K1
Secondary accession number(s): I7G121, P49992, Q59558
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 10, 2007
Last sequence update: January 9, 2007
Last modified: November 11, 2015
This is version 65 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.