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A0R7H5

- SYL_MYCS2

UniProt

A0R7H5 - SYL_MYCS2

Protein

Leucine--tRNA ligase

Gene

leuS

Organism
Mycobacterium smegmatis (strain ATCC 700084 / mc(2)155)
Status
Reviewed - Annotation score: 2 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 66 (01 Oct 2014)
      Sequence version 1 (09 Jan 2007)
      Previous versions | rss
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    Functioni

    Catalytic activityi

    ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-tRNA(Leu).UniRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei728 – 7281ATPUniRule annotation

    GO - Molecular functioni

    1. aminoacyl-tRNA editing activity Source: InterPro
    2. ATP binding Source: UniProtKB-HAMAP
    3. leucine-tRNA ligase activity Source: UniProtKB-HAMAP

    GO - Biological processi

    1. leucyl-tRNA aminoacylation Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Aminoacyl-tRNA synthetase, Ligase

    Keywords - Biological processi

    Protein biosynthesis

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding

    Enzyme and pathway databases

    BioCyciMSME246196:GJ4Y-6912-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Leucine--tRNA ligaseUniRule annotation (EC:6.1.1.4UniRule annotation)
    Alternative name(s):
    Leucyl-tRNA synthetaseUniRule annotation
    Short name:
    LeuRSUniRule annotation
    Gene namesi
    Name:leuSUniRule annotation
    Ordered Locus Names:MSMEG_6917, MSMEI_6729
    OrganismiMycobacterium smegmatis (strain ATCC 700084 / mc(2)155)
    Taxonomic identifieri246196 [NCBI]
    Taxonomic lineageiBacteriaActinobacteriaActinobacteridaeActinomycetalesCorynebacterineaeMycobacteriaceaeMycobacterium
    ProteomesiUP000000757: Chromosome, UP000006158: Chromosome

    Subcellular locationi

    Cytoplasm UniRule annotation

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 953953Leucine--tRNA ligasePRO_0000334775Add
    BLAST

    Interactioni

    Protein-protein interaction databases

    STRINGi246196.MSMEG_6917.

    Structurei

    3D structure databases

    ProteinModelPortaliA0R7H5.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi71 – 8212"HIGH" regionAdd
    BLAST
    Motifi725 – 7295"KMSKS" region

    Sequence similaritiesi

    Belongs to the class-I aminoacyl-tRNA synthetase family.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0495.
    HOGENOMiHOG000200748.
    KOiK01869.
    OMAiYARYHRM.
    OrthoDBiEOG63Z74X.

    Family and domain databases

    Gene3Di1.10.730.10. 1 hit.
    3.40.50.620. 3 hits.
    3.90.740.10. 1 hit.
    HAMAPiMF_00049_B. Leu_tRNA_synth_B.
    InterProiIPR001412. aa-tRNA-synth_I_CS.
    IPR002302. Leu-tRNA-ligase.
    IPR025709. Leu_tRNA-synth_edit.
    IPR015413. Methionyl/Leucyl_tRNA_Synth.
    IPR014729. Rossmann-like_a/b/a_fold.
    IPR009080. tRNAsynth_1a_anticodon-bd.
    IPR013155. V/L/I-tRNA-synth_anticodon-bd.
    IPR009008. Val/Leu/Ile-tRNA-synth_edit.
    [Graphical view]
    PANTHERiPTHR11946:SF7. PTHR11946:SF7. 1 hit.
    PfamiPF08264. Anticodon_1. 1 hit.
    PF13603. tRNA-synt_1_2. 1 hit.
    PF09334. tRNA-synt_1g. 1 hit.
    [Graphical view]
    PRINTSiPR00985. TRNASYNTHLEU.
    SUPFAMiSSF47323. SSF47323. 1 hit.
    SSF50677. SSF50677. 1 hit.
    TIGRFAMsiTIGR00396. leuS_bact. 1 hit.
    PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    A0R7H5-1 [UniParc]FASTAAdd to Basket

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    MTEPATTPTT PDEQIPRHRY NADLAGQIER AWQETWSDRG TFNVANPVGS    50
    LAPTDGSDVP ADKMFVQDMF PYPSGDGLHV GHPLGYIATD VYARYYRMLG 100
    RNVLHALGFD AFGLPAEQYA VQTGTHPRTR TEANIVNFRR QLGRLGLGHD 150
    TRRSFSTTDV DYYKWTQWIF LQIYNAWFDR DQNKARRISE LVEEFESGKR 200
    TLDDGRNWAD LSKGERADVI DGYRLVYRAD SMVNWCPGLG TVLANEEVTS 250
    EGRSDRGNFP VFRKRLRQWM MRITAYSDRL LEDLDVLDWP EKVKTMQRNW 300
    IGRSTGASVL FATAADDIEV FTTRPDTLFG ATYLVLAPEH DLVDTLVTDA 350
    WPDGTDERWT YGAATPREAV AAYRTDIAAK SDLERQENKT KTGVFLGAYA 400
    TNPADGKQVP IFIADYVLAG YGTGAIMAVP GGDQRDWDFA KEFGLPIIEV 450
    VTGGDISEAA YAGDGTMVNS GFLDGMDVAS AKEAIIARLE ADGRGKRRVE 500
    YKLRDWLFAR QRYWGEPFPI VYDADGRAHP LPESALPVEL PDVPDYSPVL 550
    FDPDDADSEP SPPLNKATEW VHVELDLGDG LQSYTRDTNV MPQWAGSSWY 600
    ELRYTDPHNP DEMCAKENEA YWMGPRPDEH GPEDPGGVDL YVGGVEHAVL 650
    HLLYSRFWHK VLYDLGYVSS REPYRRLVNQ GYIQAFAYTD SRGTYVPAAE 700
    VIERDGKFFW PGPDGEIEVN QEFGKIGKSL KNSVSPDEIC DNYGADTLRV 750
    YEMSMGPLEA SRPWATKDVV GAHRFLQRVW RVVIDETSGN VRVVEHEALS 800
    DETLRLLHRT IEGVREDYAA LRNNTAAAKL IEYTNHLTKE GVAARAAIEP 850
    LVLMVAPLAP HLAEELWKRL GHDTSLAHGP FPEADPQYLV EDTIEFPVQV 900
    NGKVRGKIVV AADADKAALE AAALADEKVQ AFLAGATPKK VIVVPGRLVN 950
    LVV 953
    Length:953
    Mass (Da):106,556
    Last modified:January 9, 2007 - v1
    Checksum:iFE42D3FF49C6681C
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000480 Genomic DNA. Translation: ABK71384.1.
    CP001663 Genomic DNA. Translation: AFP43155.1.
    RefSeqiYP_006571450.1. NC_018289.1.
    YP_891113.1. NC_008596.1.

    Genome annotation databases

    EnsemblBacteriaiABK71384; ABK71384; MSMEG_6917.
    AFP43155; AFP43155; MSMEI_6729.
    GeneIDi4535790.
    KEGGimsg:MSMEI_6729.
    msm:MSMEG_6917.
    PATRICi18086044. VBIMycSme59918_6734.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000480 Genomic DNA. Translation: ABK71384.1 .
    CP001663 Genomic DNA. Translation: AFP43155.1 .
    RefSeqi YP_006571450.1. NC_018289.1.
    YP_891113.1. NC_008596.1.

    3D structure databases

    ProteinModelPortali A0R7H5.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 246196.MSMEG_6917.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai ABK71384 ; ABK71384 ; MSMEG_6917 .
    AFP43155 ; AFP43155 ; MSMEI_6729 .
    GeneIDi 4535790.
    KEGGi msg:MSMEI_6729.
    msm:MSMEG_6917.
    PATRICi 18086044. VBIMycSme59918_6734.

    Phylogenomic databases

    eggNOGi COG0495.
    HOGENOMi HOG000200748.
    KOi K01869.
    OMAi YARYHRM.
    OrthoDBi EOG63Z74X.

    Enzyme and pathway databases

    BioCyci MSME246196:GJ4Y-6912-MONOMER.

    Family and domain databases

    Gene3Di 1.10.730.10. 1 hit.
    3.40.50.620. 3 hits.
    3.90.740.10. 1 hit.
    HAMAPi MF_00049_B. Leu_tRNA_synth_B.
    InterProi IPR001412. aa-tRNA-synth_I_CS.
    IPR002302. Leu-tRNA-ligase.
    IPR025709. Leu_tRNA-synth_edit.
    IPR015413. Methionyl/Leucyl_tRNA_Synth.
    IPR014729. Rossmann-like_a/b/a_fold.
    IPR009080. tRNAsynth_1a_anticodon-bd.
    IPR013155. V/L/I-tRNA-synth_anticodon-bd.
    IPR009008. Val/Leu/Ile-tRNA-synth_edit.
    [Graphical view ]
    PANTHERi PTHR11946:SF7. PTHR11946:SF7. 1 hit.
    Pfami PF08264. Anticodon_1. 1 hit.
    PF13603. tRNA-synt_1_2. 1 hit.
    PF09334. tRNA-synt_1g. 1 hit.
    [Graphical view ]
    PRINTSi PR00985. TRNASYNTHLEU.
    SUPFAMi SSF47323. SSF47323. 1 hit.
    SSF50677. SSF50677. 1 hit.
    TIGRFAMsi TIGR00396. leuS_bact. 1 hit.
    PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Fleischmann R.D., Dodson R.J., Haft D.H., Merkel J.S., Nelson W.C., Fraser C.M.
      Submitted (OCT-2006) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 700084 / mc(2)155.
    2. "Interrupted coding sequences in Mycobacterium smegmatis: authentic mutations or sequencing errors?"
      Deshayes C., Perrodou E., Gallien S., Euphrasie D., Schaeffer C., Van-Dorsselaer A., Poch O., Lecompte O., Reyrat J.-M.
      Genome Biol. 8:R20.1-R20.9(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 700084 / mc(2)155.
    3. "Ortho-proteogenomics: multiple proteomes investigation through orthology and a new MS-based protocol."
      Gallien S., Perrodou E., Carapito C., Deshayes C., Reyrat J.-M., Van Dorsselaer A., Poch O., Schaeffer C., Lecompte O.
      Genome Res. 19:128-135(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Strain: ATCC 700084 / mc(2)155.

    Entry informationi

    Entry nameiSYL_MYCS2
    AccessioniPrimary (citable) accession number: A0R7H5
    Secondary accession number(s): I7FPE6
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: May 20, 2008
    Last sequence update: January 9, 2007
    Last modified: October 1, 2014
    This is version 66 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Aminoacyl-tRNA synthetases
      List of aminoacyl-tRNA synthetase entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3