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A0R7H5

- SYL_MYCS2

UniProt

A0R7H5 - SYL_MYCS2

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Protein
Leucine--tRNA ligase
Gene
leuS, MSMEG_6917, MSMEI_6729
Organism
Mycobacterium smegmatis (strain ATCC 700084 / mc(2)155)
Status
Reviewed - Annotation score: 3 out of 5 - Experimental evidence at protein leveli

Functioni

Catalytic activityi

ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-tRNA(Leu).UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei728 – 7281ATP By similarity

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-HAMAP
  2. aminoacyl-tRNA editing activity Source: InterPro
  3. leucine-tRNA ligase activity Source: UniProtKB-HAMAP
Complete GO annotation...

GO - Biological processi

  1. leucyl-tRNA aminoacylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciMSME246196:GJ4Y-6912-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Leucine--tRNA ligase (EC:6.1.1.4)
Alternative name(s):
Leucyl-tRNA synthetase
Short name:
LeuRS
Gene namesi
Name:leuS
Ordered Locus Names:MSMEG_6917, MSMEI_6729
OrganismiMycobacterium smegmatis (strain ATCC 700084 / mc(2)155)
Taxonomic identifieri246196 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaActinobacteridaeActinomycetalesCorynebacterineaeMycobacteriaceaeMycobacterium
ProteomesiUP000000757: Chromosome, UP000006158: Chromosome

Subcellular locationi

Cytoplasm By similarity UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 953953Leucine--tRNA ligaseUniRule annotation
PRO_0000334775Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi246196.MSMEG_6917.

Structurei

3D structure databases

ProteinModelPortaliA0R7H5.

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi71 – 8212"HIGH" regionUniRule annotation
Add
BLAST
Motifi725 – 7295"KMSKS" regionUniRule annotation

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0495.
HOGENOMiHOG000200748.
KOiK01869.
OMAiYARYHRM.
OrthoDBiEOG63Z74X.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 3 hits.
3.90.740.10. 1 hit.
HAMAPiMF_00049_B. Leu_tRNA_synth_B.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002302. Leu-tRNA-ligase.
IPR025709. Leu_tRNA-synth_edit.
IPR015413. Methionyl/Leucyl_tRNA_Synth.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PANTHERiPTHR11946:SF7. PTHR11946:SF7. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF13603. tRNA-synt_1_2. 1 hit.
PF09334. tRNA-synt_1g. 1 hit.
[Graphical view]
PRINTSiPR00985. TRNASYNTHLEU.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00396. leuS_bact. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

A0R7H5-1 [UniParc]FASTAAdd to Basket

« Hide

MTEPATTPTT PDEQIPRHRY NADLAGQIER AWQETWSDRG TFNVANPVGS    50
LAPTDGSDVP ADKMFVQDMF PYPSGDGLHV GHPLGYIATD VYARYYRMLG 100
RNVLHALGFD AFGLPAEQYA VQTGTHPRTR TEANIVNFRR QLGRLGLGHD 150
TRRSFSTTDV DYYKWTQWIF LQIYNAWFDR DQNKARRISE LVEEFESGKR 200
TLDDGRNWAD LSKGERADVI DGYRLVYRAD SMVNWCPGLG TVLANEEVTS 250
EGRSDRGNFP VFRKRLRQWM MRITAYSDRL LEDLDVLDWP EKVKTMQRNW 300
IGRSTGASVL FATAADDIEV FTTRPDTLFG ATYLVLAPEH DLVDTLVTDA 350
WPDGTDERWT YGAATPREAV AAYRTDIAAK SDLERQENKT KTGVFLGAYA 400
TNPADGKQVP IFIADYVLAG YGTGAIMAVP GGDQRDWDFA KEFGLPIIEV 450
VTGGDISEAA YAGDGTMVNS GFLDGMDVAS AKEAIIARLE ADGRGKRRVE 500
YKLRDWLFAR QRYWGEPFPI VYDADGRAHP LPESALPVEL PDVPDYSPVL 550
FDPDDADSEP SPPLNKATEW VHVELDLGDG LQSYTRDTNV MPQWAGSSWY 600
ELRYTDPHNP DEMCAKENEA YWMGPRPDEH GPEDPGGVDL YVGGVEHAVL 650
HLLYSRFWHK VLYDLGYVSS REPYRRLVNQ GYIQAFAYTD SRGTYVPAAE 700
VIERDGKFFW PGPDGEIEVN QEFGKIGKSL KNSVSPDEIC DNYGADTLRV 750
YEMSMGPLEA SRPWATKDVV GAHRFLQRVW RVVIDETSGN VRVVEHEALS 800
DETLRLLHRT IEGVREDYAA LRNNTAAAKL IEYTNHLTKE GVAARAAIEP 850
LVLMVAPLAP HLAEELWKRL GHDTSLAHGP FPEADPQYLV EDTIEFPVQV 900
NGKVRGKIVV AADADKAALE AAALADEKVQ AFLAGATPKK VIVVPGRLVN 950
LVV 953
Length:953
Mass (Da):106,556
Last modified:January 9, 2007 - v1
Checksum:iFE42D3FF49C6681C
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000480 Genomic DNA. Translation: ABK71384.1.
CP001663 Genomic DNA. Translation: AFP43155.1.
RefSeqiYP_006571450.1. NC_018289.1.
YP_891113.1. NC_008596.1.

Genome annotation databases

EnsemblBacteriaiABK71384; ABK71384; MSMEG_6917.
AFP43155; AFP43155; MSMEI_6729.
GeneIDi4535790.
KEGGimsg:MSMEI_6729.
msm:MSMEG_6917.
PATRICi18086044. VBIMycSme59918_6734.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000480 Genomic DNA. Translation: ABK71384.1 .
CP001663 Genomic DNA. Translation: AFP43155.1 .
RefSeqi YP_006571450.1. NC_018289.1.
YP_891113.1. NC_008596.1.

3D structure databases

ProteinModelPortali A0R7H5.
ModBasei Search...

Protein-protein interaction databases

STRINGi 246196.MSMEG_6917.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ABK71384 ; ABK71384 ; MSMEG_6917 .
AFP43155 ; AFP43155 ; MSMEI_6729 .
GeneIDi 4535790.
KEGGi msg:MSMEI_6729.
msm:MSMEG_6917.
PATRICi 18086044. VBIMycSme59918_6734.

Phylogenomic databases

eggNOGi COG0495.
HOGENOMi HOG000200748.
KOi K01869.
OMAi YARYHRM.
OrthoDBi EOG63Z74X.

Enzyme and pathway databases

BioCyci MSME246196:GJ4Y-6912-MONOMER.

Family and domain databases

Gene3Di 1.10.730.10. 1 hit.
3.40.50.620. 3 hits.
3.90.740.10. 1 hit.
HAMAPi MF_00049_B. Leu_tRNA_synth_B.
InterProi IPR001412. aa-tRNA-synth_I_CS.
IPR002302. Leu-tRNA-ligase.
IPR025709. Leu_tRNA-synth_edit.
IPR015413. Methionyl/Leucyl_tRNA_Synth.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view ]
PANTHERi PTHR11946:SF7. PTHR11946:SF7. 1 hit.
Pfami PF08264. Anticodon_1. 1 hit.
PF13603. tRNA-synt_1_2. 1 hit.
PF09334. tRNA-synt_1g. 1 hit.
[Graphical view ]
PRINTSi PR00985. TRNASYNTHLEU.
SUPFAMi SSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsi TIGR00396. leuS_bact. 1 hit.
PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Fleischmann R.D., Dodson R.J., Haft D.H., Merkel J.S., Nelson W.C., Fraser C.M.
    Submitted (OCT-2006) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 700084 / mc(2)155.
  2. "Interrupted coding sequences in Mycobacterium smegmatis: authentic mutations or sequencing errors?"
    Deshayes C., Perrodou E., Gallien S., Euphrasie D., Schaeffer C., Van-Dorsselaer A., Poch O., Lecompte O., Reyrat J.-M.
    Genome Biol. 8:R20.1-R20.9(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 700084 / mc(2)155.
  3. "Ortho-proteogenomics: multiple proteomes investigation through orthology and a new MS-based protocol."
    Gallien S., Perrodou E., Carapito C., Deshayes C., Reyrat J.-M., Van Dorsselaer A., Poch O., Schaeffer C., Lecompte O.
    Genome Res. 19:128-135(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Strain: ATCC 700084 / mc(2)155.

Entry informationi

Entry nameiSYL_MYCS2
AccessioniPrimary (citable) accession number: A0R7H5
Secondary accession number(s): I7FPE6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 20, 2008
Last sequence update: January 9, 2007
Last modified: July 9, 2014
This is version 65 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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