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A0R7G2 (PBP1A_MYCS2) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 51. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Penicillin-binding protein 1A
Gene names
Name:ponA1
Ordered Locus Names:MSMEG_6900, MSMEI_6716
OrganismMycobacterium smegmatis (strain ATCC 700084 / mc(2)155) [Reference proteome] [HAMAP]
Taxonomic identifier246196 [NCBI]
Taxonomic lineageBacteriaActinobacteriaActinobacteridaeActinomycetalesCorynebacterineaeMycobacteriaceaeMycobacterium

Protein attributes

Sequence length785 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Cell wall formation. Synthesis of cross-linked peptidoglycan from the lipid intermediates. The enzyme has a penicillin-insensitive transglycosylase N-terminal domain (formation of linear glycan strands) and a penicillin-sensitive transpeptidase C-terminal domain (cross-linking of the peptide subunits) By similarity.

Pathway

Cell wall biogenesis; peptidoglycan biosynthesis.

Subcellular location

Cell membrane Potential; Single-pass membrane protein Potential.

Disruption phenotype

Probably essential. In a depletion mutant growth rate is dramatically impaired, cells are short with bulbous poles and/or rounded internal regions. Ref.4

Sequence caution

The sequence AFP43142.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.

Ontologies

Keywords
   Cellular componentCell membrane
Membrane
   DomainTransmembrane
Transmembrane helix
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processpeptidoglycan biosynthetic process

Inferred from electronic annotation. Source: UniProtKB-UniPathway

   Cellular_componentintegral component of membrane

Inferred from electronic annotation. Source: UniProtKB-KW

plasma membrane

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionpenicillin binding

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 785785Penicillin-binding protein 1A
PRO_0000421125

Regions

Transmembrane77 – 9721Helical; Potential
Region118 – 299182Transglycosylase
Region392 – 662271Transpeptidase
Compositional bias2 – 6059Pro-rich
Compositional bias705 – 78581Pro-rich

Sites

Active site4261Acyl-ester intermediate By similarity

Sequences

Sequence LengthMass (Da)Tools
A0R7G2 [UniParc].

Last modified January 9, 2007. Version 1.
Checksum: A69C687EC368DF04

FASTA78581,567
        10         20         30         40         50         60 
MPPDDRLTAV LPPVRDGDIA PPIDVVRAAL EGSPPPKPPP PPPPGRGGGG PSGPGGPSGP 

        70         80         90        100        110        120 
GRQFRINWKW VRRGSAIAVA VMVLLPLITF GMAYMIVDVP EPGDIRTPQV STILASDGSE 

       130        140        150        160        170        180 
IARIVPPEGN RVDVKIDQIP VHVRDAVMAA EDRDFYSNPG FSWTGFLRAI KNNLFGGGGL 

       190        200        210        220        230        240 
QGGSTITQQY VKNALVGDER SGIGGLIRKA KELVISTKMS GEWSKDAVLQ AYLNIIYFGR 

       250        260        270        280        290        300 
GAYGISAASK AYFDKPVEQL DVAEGALLAA LIQRPSTLDP AVDPEGAADR WNWVLDGMVD 

       310        320        330        340        350        360 
IGALSQADRD AQVFPPTVPP DYAFQQNQTT GPNGLIERQV TNELLDLFDI NEQTLNTEGL 

       370        380        390        400        410        420 
QITTTIDPKA QEAAVDAVDK YLEGQDPDMR AAVVSIDPRT GGIKAYYGGS DANGFDFAQA 

       430        440        450        460        470        480 
GLPTGSSFKV FALVAALQQG IGLGYQIDSG PLEVNGIKIG NVEGEGCGTC SIAEALKRSL 

       490        500        510        520        530        540 
NTSYYRLMLK LENGPADVAE AAHDAGVAES FPGVEHTLSE DGKGGPPNNG VVLGQYQSRV 

       550        560        570        580        590        600 
LDMASAYATL AASGVYHKPH FVEKVVNSSG QVLFDASKED NGEERIDKAV ADNVTSAMQP 

       610        620        630        640        650        660 
IAGWSRGHNL AGGRPSAAKT GTVQLGDTGD NRDAWMVGYT PSLSTAVWVG TTEGVKPLVN 

       670        680        690        700        710        720 
KWGSPIYGSG LPSDIWKATM DGALEGTDVE SFPKPTEIGG YAGVPQAPAA PPPSAGPPTD 

       730        740        750        760        770        780 
PGQPSVTVIQ PTIEVAPGIT IPIGPPTTVP VGPPPGAPGA PVGPGAPEVP VAPGAPGVPG 


APPPP 

« Hide

References

« Hide 'large scale' references
[1]Fleischmann R.D., Dodson R.J., Haft D.H., Merkel J.S., Nelson W.C., Fraser C.M.
Submitted (OCT-2006) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 700084 / mc(2)155.
[2]"Interrupted coding sequences in Mycobacterium smegmatis: authentic mutations or sequencing errors?"
Deshayes C., Perrodou E., Gallien S., Euphrasie D., Schaeffer C., Van-Dorsselaer A., Poch O., Lecompte O., Reyrat J.-M.
Genome Biol. 8:R20.1-R20.9(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 700084 / mc(2)155.
[3]"Ortho-proteogenomics: multiple proteomes investigation through orthology and a new MS-based protocol."
Gallien S., Perrodou E., Carapito C., Deshayes C., Reyrat J.-M., Van Dorsselaer A., Poch O., Schaeffer C., Lecompte O.
Genome Res. 19:128-135(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 700084 / mc(2)155.
[4]"Interaction and modulation of two antagonistic cell wall enzymes of mycobacteria."
Hett E.C., Chao M.C., Rubin E.J.
PLoS Pathog. 6:E1001020-E1001020(2010) [PubMed] [Europe PMC] [Abstract]
Cited for: DISRUPTION PHENOTYPE.
Strain: ATCC 700084 / mc(2)155.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP000480 Genomic DNA. Translation: ABK70639.1.
CP001663 Genomic DNA. Translation: AFP43142.1. Different initiation.
RefSeqYP_006571437.1. NC_018289.1.
YP_891100.1. NC_008596.1.

3D structure databases

ProteinModelPortalA0R7G2.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING246196.MSMEG_6900.

Protein family/group databases

CAZyGT51. Glycosyltransferase Family 51.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaABK70639; ABK70639; MSMEG_6900.
AFP43142; AFP43142; MSMEI_6716.
GeneID13425840.
4536904.
KEGGmsg:MSMEI_6716.
msm:MSMEG_6900.
PATRIC18086018. VBIMycSme59918_6721.

Phylogenomic databases

eggNOGCOG0744.
HOGENOMHOG000041137.
OMANDAMMED.
OrthoDBEOG6CGCKC.
ProtClustDBCLSK799413.

Enzyme and pathway databases

BioCycMSME246196:GJ4Y-6899-MONOMER.
UniPathwayUPA00219.

Family and domain databases

Gene3D3.40.710.10. 1 hit.
InterProIPR012338. Beta-lactam/transpept-like.
IPR001264. Glyco_trans_51.
IPR023346. Lysozyme-like_dom.
IPR001460. PCN-bd_Tpept.
[Graphical view]
PfamPF00912. Transgly. 1 hit.
PF00905. Transpeptidase. 1 hit.
[Graphical view]
SUPFAMSSF53955. SSF53955. 1 hit.
SSF56601. SSF56601. 2 hits.
ProtoNetSearch...

Entry information

Entry namePBP1A_MYCS2
AccessionPrimary (citable) accession number: A0R7G2
Secondary accession number(s): I7GBX5
Entry history
Integrated into UniProtKB/Swiss-Prot: February 6, 2013
Last sequence update: January 9, 2007
Last modified: February 19, 2014
This is version 51 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

PATHWAY comments

Index of metabolic and biosynthesis pathways