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A0R7G2

- PBP1A_MYCS2

UniProt

A0R7G2 - PBP1A_MYCS2

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Protein

Penicillin-binding protein 1A

Gene
ponA1, MSMEG_6900, MSMEI_6716
Organism
Mycobacterium smegmatis (strain ATCC 700084 / mc(2)155)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Cell wall formation. Synthesis of cross-linked peptidoglycan from the lipid intermediates. The enzyme has a penicillin-insensitive transglycosylase N-terminal domain (formation of linear glycan strands) and a penicillin-sensitive transpeptidase C-terminal domain (cross-linking of the peptide subunits) By similarity.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei426 – 4261Acyl-ester intermediate By similarity

GO - Molecular functioni

  1. penicillin binding Source: InterPro

GO - Biological processi

  1. peptidoglycan biosynthetic process Source: UniProtKB-UniPathway
Complete GO annotation...

Enzyme and pathway databases

BioCyciMSME246196:GJ4Y-6899-MONOMER.
UniPathwayiUPA00219.

Protein family/group databases

CAZyiGT51. Glycosyltransferase Family 51.

Names & Taxonomyi

Protein namesi
Recommended name:
Penicillin-binding protein 1A
Gene namesi
Name:ponA1
Ordered Locus Names:MSMEG_6900, MSMEI_6716
OrganismiMycobacterium smegmatis (strain ATCC 700084 / mc(2)155)
Taxonomic identifieri246196 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaActinobacteridaeActinomycetalesCorynebacterineaeMycobacteriaceaeMycobacterium
ProteomesiUP000000757: Chromosome, UP000006158: Chromosome

Subcellular locationi

Cell membrane Reviewed prediction; Single-pass membrane protein Reviewed prediction

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei77 – 9721Helical; Reviewed predictionAdd
BLAST

GO - Cellular componenti

  1. integral component of membrane Source: UniProtKB-KW
  2. plasma membrane Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

Pathology & Biotechi

Disruption phenotypei

Probably essential. In a depletion mutant growth rate is dramatically impaired, cells are short with bulbous poles and/or rounded internal regions.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 785785Penicillin-binding protein 1APRO_0000421125Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi246196.MSMEG_6900.

Structurei

3D structure databases

ProteinModelPortaliA0R7G2.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni118 – 299182TransglycosylaseAdd
BLAST
Regioni392 – 662271TranspeptidaseAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi2 – 6059Pro-richAdd
BLAST
Compositional biasi705 – 78581Pro-richAdd
BLAST

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiCOG0744.
HOGENOMiHOG000041137.
OMAiKWVRRGS.
OrthoDBiEOG6CGCKC.

Family and domain databases

Gene3Di3.40.710.10. 1 hit.
InterProiIPR012338. Beta-lactam/transpept-like.
IPR001264. Glyco_trans_51.
IPR023346. Lysozyme-like_dom.
IPR001460. PCN-bd_Tpept.
[Graphical view]
PfamiPF00912. Transgly. 1 hit.
PF00905. Transpeptidase. 1 hit.
[Graphical view]
SUPFAMiSSF53955. SSF53955. 1 hit.
SSF56601. SSF56601. 2 hits.

Sequencei

Sequence statusi: Complete.

A0R7G2-1 [UniParc]FASTAAdd to Basket

« Hide

MPPDDRLTAV LPPVRDGDIA PPIDVVRAAL EGSPPPKPPP PPPPGRGGGG    50
PSGPGGPSGP GRQFRINWKW VRRGSAIAVA VMVLLPLITF GMAYMIVDVP 100
EPGDIRTPQV STILASDGSE IARIVPPEGN RVDVKIDQIP VHVRDAVMAA 150
EDRDFYSNPG FSWTGFLRAI KNNLFGGGGL QGGSTITQQY VKNALVGDER 200
SGIGGLIRKA KELVISTKMS GEWSKDAVLQ AYLNIIYFGR GAYGISAASK 250
AYFDKPVEQL DVAEGALLAA LIQRPSTLDP AVDPEGAADR WNWVLDGMVD 300
IGALSQADRD AQVFPPTVPP DYAFQQNQTT GPNGLIERQV TNELLDLFDI 350
NEQTLNTEGL QITTTIDPKA QEAAVDAVDK YLEGQDPDMR AAVVSIDPRT 400
GGIKAYYGGS DANGFDFAQA GLPTGSSFKV FALVAALQQG IGLGYQIDSG 450
PLEVNGIKIG NVEGEGCGTC SIAEALKRSL NTSYYRLMLK LENGPADVAE 500
AAHDAGVAES FPGVEHTLSE DGKGGPPNNG VVLGQYQSRV LDMASAYATL 550
AASGVYHKPH FVEKVVNSSG QVLFDASKED NGEERIDKAV ADNVTSAMQP 600
IAGWSRGHNL AGGRPSAAKT GTVQLGDTGD NRDAWMVGYT PSLSTAVWVG 650
TTEGVKPLVN KWGSPIYGSG LPSDIWKATM DGALEGTDVE SFPKPTEIGG 700
YAGVPQAPAA PPPSAGPPTD PGQPSVTVIQ PTIEVAPGIT IPIGPPTTVP 750
VGPPPGAPGA PVGPGAPEVP VAPGAPGVPG APPPP 785
Length:785
Mass (Da):81,567
Last modified:January 9, 2007 - v1
Checksum:iA69C687EC368DF04
GO

Sequence cautioni

The sequence AFP43142.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000480 Genomic DNA. Translation: ABK70639.1.
CP001663 Genomic DNA. Translation: AFP43142.1. Different initiation.
RefSeqiWP_011731616.1. NC_008596.1.
YP_006571437.1. NC_018289.1.
YP_891100.1. NC_008596.1.

Genome annotation databases

EnsemblBacteriaiABK70639; ABK70639; MSMEG_6900.
AFP43142; AFP43142; MSMEI_6716.
GeneIDi4536904.
KEGGimsg:MSMEI_6716.
msm:MSMEG_6900.
PATRICi18086018. VBIMycSme59918_6721.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000480 Genomic DNA. Translation: ABK70639.1 .
CP001663 Genomic DNA. Translation: AFP43142.1 . Different initiation.
RefSeqi WP_011731616.1. NC_008596.1.
YP_006571437.1. NC_018289.1.
YP_891100.1. NC_008596.1.

3D structure databases

ProteinModelPortali A0R7G2.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 246196.MSMEG_6900.

Protein family/group databases

CAZyi GT51. Glycosyltransferase Family 51.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ABK70639 ; ABK70639 ; MSMEG_6900 .
AFP43142 ; AFP43142 ; MSMEI_6716 .
GeneIDi 4536904.
KEGGi msg:MSMEI_6716.
msm:MSMEG_6900.
PATRICi 18086018. VBIMycSme59918_6721.

Phylogenomic databases

eggNOGi COG0744.
HOGENOMi HOG000041137.
OMAi KWVRRGS.
OrthoDBi EOG6CGCKC.

Enzyme and pathway databases

UniPathwayi UPA00219 .
BioCyci MSME246196:GJ4Y-6899-MONOMER.

Family and domain databases

Gene3Di 3.40.710.10. 1 hit.
InterProi IPR012338. Beta-lactam/transpept-like.
IPR001264. Glyco_trans_51.
IPR023346. Lysozyme-like_dom.
IPR001460. PCN-bd_Tpept.
[Graphical view ]
Pfami PF00912. Transgly. 1 hit.
PF00905. Transpeptidase. 1 hit.
[Graphical view ]
SUPFAMi SSF53955. SSF53955. 1 hit.
SSF56601. SSF56601. 2 hits.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. Fleischmann R.D., Dodson R.J., Haft D.H., Merkel J.S., Nelson W.C., Fraser C.M.
    Submitted (OCT-2006) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 700084 / mc(2)155.
  2. "Interrupted coding sequences in Mycobacterium smegmatis: authentic mutations or sequencing errors?"
    Deshayes C., Perrodou E., Gallien S., Euphrasie D., Schaeffer C., Van-Dorsselaer A., Poch O., Lecompte O., Reyrat J.-M.
    Genome Biol. 8:R20.1-R20.9(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 700084 / mc(2)155.
  3. "Ortho-proteogenomics: multiple proteomes investigation through orthology and a new MS-based protocol."
    Gallien S., Perrodou E., Carapito C., Deshayes C., Reyrat J.-M., Van Dorsselaer A., Poch O., Schaeffer C., Lecompte O.
    Genome Res. 19:128-135(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 700084 / mc(2)155.
  4. "Interaction and modulation of two antagonistic cell wall enzymes of mycobacteria."
    Hett E.C., Chao M.C., Rubin E.J.
    PLoS Pathog. 6:E1001020-E1001020(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: DISRUPTION PHENOTYPE.
    Strain: ATCC 700084 / mc(2)155.

Entry informationi

Entry nameiPBP1A_MYCS2
AccessioniPrimary (citable) accession number: A0R7G2
Secondary accession number(s): I7GBX5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 6, 2013
Last sequence update: January 9, 2007
Last modified: September 3, 2014
This is version 54 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways

External Data

Dasty 3

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