Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

A0R7G2

- PBP1A_MYCS2

UniProt

A0R7G2 - PBP1A_MYCS2

Protein

Penicillin-binding protein 1A

Gene

ponA1

Organism
Mycobacterium smegmatis (strain ATCC 700084 / mc(2)155)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
    • BLAST
    • Align
    • Format
    • Add to basket
    • History
      Entry version 55 (01 Oct 2014)
      Sequence version 1 (09 Jan 2007)
      Previous versions | rss
    • Help video
    • Feedback
    • Comment

    Functioni

    Cell wall formation. Synthesis of cross-linked peptidoglycan from the lipid intermediates. The enzyme has a penicillin-insensitive transglycosylase N-terminal domain (formation of linear glycan strands) and a penicillin-sensitive transpeptidase C-terminal domain (cross-linking of the peptide subunits) By similarity.By similarity

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei426 – 4261Acyl-ester intermediateBy similarity

    GO - Molecular functioni

    1. penicillin binding Source: InterPro

    GO - Biological processi

    1. peptidoglycan biosynthetic process Source: UniProtKB-UniPathway

    Enzyme and pathway databases

    BioCyciMSME246196:GJ4Y-6899-MONOMER.
    UniPathwayiUPA00219.

    Protein family/group databases

    CAZyiGT51. Glycosyltransferase Family 51.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Penicillin-binding protein 1A
    Gene namesi
    Name:ponA1
    Ordered Locus Names:MSMEG_6900, MSMEI_6716
    OrganismiMycobacterium smegmatis (strain ATCC 700084 / mc(2)155)
    Taxonomic identifieri246196 [NCBI]
    Taxonomic lineageiBacteriaActinobacteriaActinobacteridaeActinomycetalesCorynebacterineaeMycobacteriaceaeMycobacterium
    ProteomesiUP000000757: Chromosome, UP000006158: Chromosome

    Subcellular locationi

    GO - Cellular componenti

    1. integral component of membrane Source: UniProtKB-KW
    2. plasma membrane Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cell membrane, Membrane

    Pathology & Biotechi

    Disruption phenotypei

    Probably essential. In a depletion mutant growth rate is dramatically impaired, cells are short with bulbous poles and/or rounded internal regions.1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 785785Penicillin-binding protein 1APRO_0000421125Add
    BLAST

    Interactioni

    Protein-protein interaction databases

    STRINGi246196.MSMEG_6900.

    Structurei

    3D structure databases

    ProteinModelPortaliA0R7G2.
    ModBaseiSearch...
    MobiDBiSearch...

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei77 – 9721HelicalSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni118 – 299182TransglycosylaseAdd
    BLAST
    Regioni392 – 662271TranspeptidaseAdd
    BLAST

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi2 – 6059Pro-richAdd
    BLAST
    Compositional biasi705 – 78581Pro-richAdd
    BLAST

    Keywords - Domaini

    Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiCOG0744.
    HOGENOMiHOG000041137.
    OMAiKWVRRGS.
    OrthoDBiEOG6CGCKC.

    Family and domain databases

    Gene3Di3.40.710.10. 1 hit.
    InterProiIPR012338. Beta-lactam/transpept-like.
    IPR001264. Glyco_trans_51.
    IPR023346. Lysozyme-like_dom.
    IPR001460. PCN-bd_Tpept.
    [Graphical view]
    PfamiPF00912. Transgly. 1 hit.
    PF00905. Transpeptidase. 1 hit.
    [Graphical view]
    SUPFAMiSSF53955. SSF53955. 1 hit.
    SSF56601. SSF56601. 2 hits.

    Sequencei

    Sequence statusi: Complete.

    A0R7G2-1 [UniParc]FASTAAdd to Basket

    « Hide

    MPPDDRLTAV LPPVRDGDIA PPIDVVRAAL EGSPPPKPPP PPPPGRGGGG    50
    PSGPGGPSGP GRQFRINWKW VRRGSAIAVA VMVLLPLITF GMAYMIVDVP 100
    EPGDIRTPQV STILASDGSE IARIVPPEGN RVDVKIDQIP VHVRDAVMAA 150
    EDRDFYSNPG FSWTGFLRAI KNNLFGGGGL QGGSTITQQY VKNALVGDER 200
    SGIGGLIRKA KELVISTKMS GEWSKDAVLQ AYLNIIYFGR GAYGISAASK 250
    AYFDKPVEQL DVAEGALLAA LIQRPSTLDP AVDPEGAADR WNWVLDGMVD 300
    IGALSQADRD AQVFPPTVPP DYAFQQNQTT GPNGLIERQV TNELLDLFDI 350
    NEQTLNTEGL QITTTIDPKA QEAAVDAVDK YLEGQDPDMR AAVVSIDPRT 400
    GGIKAYYGGS DANGFDFAQA GLPTGSSFKV FALVAALQQG IGLGYQIDSG 450
    PLEVNGIKIG NVEGEGCGTC SIAEALKRSL NTSYYRLMLK LENGPADVAE 500
    AAHDAGVAES FPGVEHTLSE DGKGGPPNNG VVLGQYQSRV LDMASAYATL 550
    AASGVYHKPH FVEKVVNSSG QVLFDASKED NGEERIDKAV ADNVTSAMQP 600
    IAGWSRGHNL AGGRPSAAKT GTVQLGDTGD NRDAWMVGYT PSLSTAVWVG 650
    TTEGVKPLVN KWGSPIYGSG LPSDIWKATM DGALEGTDVE SFPKPTEIGG 700
    YAGVPQAPAA PPPSAGPPTD PGQPSVTVIQ PTIEVAPGIT IPIGPPTTVP 750
    VGPPPGAPGA PVGPGAPEVP VAPGAPGVPG APPPP 785
    Length:785
    Mass (Da):81,567
    Last modified:January 9, 2007 - v1
    Checksum:iA69C687EC368DF04
    GO

    Sequence cautioni

    The sequence AFP43142.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000480 Genomic DNA. Translation: ABK70639.1.
    CP001663 Genomic DNA. Translation: AFP43142.1. Different initiation.
    RefSeqiWP_011731616.1. NC_008596.1.
    YP_006571437.1. NC_018289.1.
    YP_891100.1. NC_008596.1.

    Genome annotation databases

    EnsemblBacteriaiABK70639; ABK70639; MSMEG_6900.
    AFP43142; AFP43142; MSMEI_6716.
    GeneIDi4536904.
    KEGGimsg:MSMEI_6716.
    msm:MSMEG_6900.
    PATRICi18086018. VBIMycSme59918_6721.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000480 Genomic DNA. Translation: ABK70639.1 .
    CP001663 Genomic DNA. Translation: AFP43142.1 . Different initiation.
    RefSeqi WP_011731616.1. NC_008596.1.
    YP_006571437.1. NC_018289.1.
    YP_891100.1. NC_008596.1.

    3D structure databases

    ProteinModelPortali A0R7G2.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 246196.MSMEG_6900.

    Protein family/group databases

    CAZyi GT51. Glycosyltransferase Family 51.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai ABK70639 ; ABK70639 ; MSMEG_6900 .
    AFP43142 ; AFP43142 ; MSMEI_6716 .
    GeneIDi 4536904.
    KEGGi msg:MSMEI_6716.
    msm:MSMEG_6900.
    PATRICi 18086018. VBIMycSme59918_6721.

    Phylogenomic databases

    eggNOGi COG0744.
    HOGENOMi HOG000041137.
    OMAi KWVRRGS.
    OrthoDBi EOG6CGCKC.

    Enzyme and pathway databases

    UniPathwayi UPA00219 .
    BioCyci MSME246196:GJ4Y-6899-MONOMER.

    Family and domain databases

    Gene3Di 3.40.710.10. 1 hit.
    InterProi IPR012338. Beta-lactam/transpept-like.
    IPR001264. Glyco_trans_51.
    IPR023346. Lysozyme-like_dom.
    IPR001460. PCN-bd_Tpept.
    [Graphical view ]
    Pfami PF00912. Transgly. 1 hit.
    PF00905. Transpeptidase. 1 hit.
    [Graphical view ]
    SUPFAMi SSF53955. SSF53955. 1 hit.
    SSF56601. SSF56601. 2 hits.
    ProtoNeti Search...

    Publicationsi

    1. Fleischmann R.D., Dodson R.J., Haft D.H., Merkel J.S., Nelson W.C., Fraser C.M.
      Submitted (OCT-2006) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 700084 / mc(2)155.
    2. "Interrupted coding sequences in Mycobacterium smegmatis: authentic mutations or sequencing errors?"
      Deshayes C., Perrodou E., Gallien S., Euphrasie D., Schaeffer C., Van-Dorsselaer A., Poch O., Lecompte O., Reyrat J.-M.
      Genome Biol. 8:R20.1-R20.9(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 700084 / mc(2)155.
    3. "Ortho-proteogenomics: multiple proteomes investigation through orthology and a new MS-based protocol."
      Gallien S., Perrodou E., Carapito C., Deshayes C., Reyrat J.-M., Van Dorsselaer A., Poch O., Schaeffer C., Lecompte O.
      Genome Res. 19:128-135(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 700084 / mc(2)155.
    4. "Interaction and modulation of two antagonistic cell wall enzymes of mycobacteria."
      Hett E.C., Chao M.C., Rubin E.J.
      PLoS Pathog. 6:E1001020-E1001020(2010) [PubMed] [Europe PMC] [Abstract]
      Cited for: DISRUPTION PHENOTYPE.
      Strain: ATCC 700084 / mc(2)155.

    Entry informationi

    Entry nameiPBP1A_MYCS2
    AccessioniPrimary (citable) accession number: A0R7G2
    Secondary accession number(s): I7GBX5
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 6, 2013
    Last sequence update: January 9, 2007
    Last modified: October 1, 2014
    This is version 55 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways

    External Data

    Dasty 3