A0R7G2 (PBP1A_MYCS2) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Penicillin-binding protein 1A

Gene names

Name:	ponA1
Ordered Locus Names:	MSMEG_6900, MSMEI_6716

Organism

Mycobacterium smegmatis (strain ATCC 700084 / mc(2)155) [Reference proteome] [HAMAP]

Taxonomic identifier

246196 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Actinobacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Corynebacterineae › Mycobacteriaceae › Mycobacterium › Mycobacterium smegmatis

Protein attributes

Sequence length	785 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Cell wall formation. Synthesis of cross-linked peptidoglycan from the lipid intermediates. The enzyme has a penicillin-insensitive transglycosylase N-terminal domain (formation of linear glycan strands) and a penicillin-sensitive transpeptidase C-terminal domain (cross-linking of the peptide subunits) By similarity.
Pathway	Cell wall biogenesis; peptidoglycan biosynthesis.
Subcellular location	Cell membrane Potential; Single-pass membrane protein Potential.
Disruption phenotype	Probably essential. In a depletion mutant growth rate is dramatically impaired, cells are short with bulbous poles and/or rounded internal regions. Ref.4
Sequence caution	The sequence AFP43142.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.

Ontologies

Keywords
Cellular component	Cell membrane Membrane
Domain	Transmembrane Transmembrane helix
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	peptidoglycan biosynthetic process Inferred from electronic annotation. Source: UniProtKB-UniPathway
Cellular_component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	penicillin binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 785	785	Penicillin-binding protein 1A		PRO_0000421125
Regions
	Transmembrane	77 – 97	21	Helical; Potential
	Region	118 – 299	182	Transglycosylase
	Region	392 – 662	271	Transpeptidase
	Compositional bias	2 – 60	59	Pro-rich
	Compositional bias	705 – 785	81	Pro-rich
Sites
	Active site	426	1	Acyl-ester intermediate By similarity

Sequences

	Sequence		Length	Mass (Da)
	A0R7G2 [UniParc]. Last modified January 9, 2007. Version 1. Checksum: A69C687EC368DF04	FASTA	785	81,567
	10 20 30 40 50 60 MPPDDRLTAV LPPVRDGDIA PPIDVVRAAL EGSPPPKPPP PPPPGRGGGG PSGPGGPSGP 70 80 90 100 110 120 GRQFRINWKW VRRGSAIAVA VMVLLPLITF GMAYMIVDVP EPGDIRTPQV STILASDGSE 130 140 150 160 170 180 IARIVPPEGN RVDVKIDQIP VHVRDAVMAA EDRDFYSNPG FSWTGFLRAI KNNLFGGGGL 190 200 210 220 230 240 QGGSTITQQY VKNALVGDER SGIGGLIRKA KELVISTKMS GEWSKDAVLQ AYLNIIYFGR 250 260 270 280 290 300 GAYGISAASK AYFDKPVEQL DVAEGALLAA LIQRPSTLDP AVDPEGAADR WNWVLDGMVD 310 320 330 340 350 360 IGALSQADRD AQVFPPTVPP DYAFQQNQTT GPNGLIERQV TNELLDLFDI NEQTLNTEGL 370 380 390 400 410 420 QITTTIDPKA QEAAVDAVDK YLEGQDPDMR AAVVSIDPRT GGIKAYYGGS DANGFDFAQA 430 440 450 460 470 480 GLPTGSSFKV FALVAALQQG IGLGYQIDSG PLEVNGIKIG NVEGEGCGTC SIAEALKRSL 490 500 510 520 530 540 NTSYYRLMLK LENGPADVAE AAHDAGVAES FPGVEHTLSE DGKGGPPNNG VVLGQYQSRV 550 560 570 580 590 600 LDMASAYATL AASGVYHKPH FVEKVVNSSG QVLFDASKED NGEERIDKAV ADNVTSAMQP 610 620 630 640 650 660 IAGWSRGHNL AGGRPSAAKT GTVQLGDTGD NRDAWMVGYT PSLSTAVWVG TTEGVKPLVN 670 680 690 700 710 720 KWGSPIYGSG LPSDIWKATM DGALEGTDVE SFPKPTEIGG YAGVPQAPAA PPPSAGPPTD 730 740 750 760 770 780 PGQPSVTVIQ PTIEVAPGIT IPIGPPTTVP VGPPPGAPGA PVGPGAPEVP VAPGAPGVPG APPPP

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	Fleischmann R.D., Dodson R.J., Haft D.H., Merkel J.S., Nelson W.C., Fraser C.M. Submitted (OCT-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 700084 / mc(2)155.
[2]	"Interrupted coding sequences in Mycobacterium smegmatis: authentic mutations or sequencing errors?" Deshayes C., Perrodou E., Gallien S., Euphrasie D., Schaeffer C., Van-Dorsselaer A., Poch O., Lecompte O., Reyrat J.-M. Genome Biol. 8:R20.1-R20.9(2007) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 700084 / mc(2)155.
[3]	"Ortho-proteogenomics: multiple proteomes investigation through orthology and a new MS-based protocol." Gallien S., Perrodou E., Carapito C., Deshayes C., Reyrat J.-M., Van Dorsselaer A., Poch O., Schaeffer C., Lecompte O. Genome Res. 19:128-135(2009) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 700084 / mc(2)155.
[4]	"Interaction and modulation of two antagonistic cell wall enzymes of mycobacteria." Hett E.C., Chao M.C., Rubin E.J. PLoS Pathog. 6:E1001020-E1001020(2010) [PubMed] [Europe PMC] [Abstract] Cited for: DISRUPTION PHENOTYPE. Strain: ATCC 700084 / mc(2)155.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000480 Genomic DNA. Translation: ABK70639.1. CP001663 Genomic DNA. Translation: AFP43142.1. Different initiation.
RefSeq	YP_006571437.1. NC_018289.1. YP_891100.1. NC_008596.1.
3D structure databases
ProteinModelPortal	A0R7G2.
ModBase	Search...
Protein-protein interaction databases
STRING	246196.MSMEG_6900.
Protein family/group databases
CAZy	GT51. Glycosyltransferase Family 51.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	ABK70639; ABK70639; MSMEG_6900.
GeneID	13425840. 4536904.
KEGG	msg:MSMEI_6716. msm:MSMEG_6900.
PATRIC	18086018. VBIMycSme59918_6721.
Phylogenomic databases
eggNOG	COG0744.
HOGENOM	HOG000041137.
OMA	HETHIVE.
ProtClustDB	CLSK799413.
Enzyme and pathway databases
BioCyc	MSME246196:GJ4Y-6899-MONOMER.
UniPathway	UPA00219.
Family and domain databases
Gene3D	3.40.710.10. 1 hit.
InterPro	IPR012338. Beta-lactam/transpept-like. IPR001264. Glyco_trans_51. IPR001460. PCN-bd_Tpept. [Graphical view]
Pfam	PF00912. Transgly. 1 hit. PF00905. Transpeptidase. 1 hit. [Graphical view]
SUPFAM	SSF56601. PBP_transp_fold. 1 hit.
ProtoNet	Search...

Entry information

Entry name

PBP1A_MYCS2

Accession

Primary (citable) accession number: A0R7G2
Secondary accession number(s): I7GBX5

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 6, 2013
Last sequence update:	January 9, 2007
Last modified:	May 29, 2013
This is version 48 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program