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A0R7G2

- PBP1A_MYCS2

UniProt

A0R7G2 - PBP1A_MYCS2

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Protein

Penicillin-binding protein 1A

Gene

ponA1

Organism
Mycobacterium smegmatis (strain ATCC 700084 / mc(2)155)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

Cell wall formation. Synthesis of cross-linked peptidoglycan from the lipid intermediates. The enzyme has a penicillin-insensitive transglycosylase N-terminal domain (formation of linear glycan strands) and a penicillin-sensitive transpeptidase C-terminal domain (cross-linking of the peptide subunits) (By similarity).By similarity

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei426 – 4261Acyl-ester intermediateBy similarity

GO - Molecular functioni

  1. penicillin binding Source: InterPro

GO - Biological processi

  1. peptidoglycan biosynthetic process Source: UniProtKB-UniPathway
Complete GO annotation...

Enzyme and pathway databases

BioCyciMSME246196:GJ4Y-6899-MONOMER.
UniPathwayiUPA00219.

Protein family/group databases

CAZyiGT51. Glycosyltransferase Family 51.

Names & Taxonomyi

Protein namesi
Recommended name:
Penicillin-binding protein 1A
Gene namesi
Name:ponA1
Ordered Locus Names:MSMEG_6900, MSMEI_6716
OrganismiMycobacterium smegmatis (strain ATCC 700084 / mc(2)155)
Taxonomic identifieri246196 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaActinobacteridaeActinomycetalesCorynebacterineaeMycobacteriaceaeMycobacterium
ProteomesiUP000000757: Chromosome, UP000006158: Chromosome

Subcellular locationi

GO - Cellular componenti

  1. integral component of membrane Source: UniProtKB-KW
  2. plasma membrane Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

Pathology & Biotechi

Disruption phenotypei

Probably essential. In a depletion mutant growth rate is dramatically impaired, cells are short with bulbous poles and/or rounded internal regions.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 785785Penicillin-binding protein 1APRO_0000421125Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi246196.MSMEG_6900.

Structurei

3D structure databases

ProteinModelPortaliA0R7G2.
ModBaseiSearch...
MobiDBiSearch...

Transmembrane

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei77 – 9721HelicalSequence AnalysisAdd
BLAST

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni118 – 299182TransglycosylaseAdd
BLAST
Regioni392 – 662271TranspeptidaseAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi2 – 6059Pro-richAdd
BLAST
Compositional biasi705 – 78581Pro-richAdd
BLAST

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiCOG0744.
HOGENOMiHOG000041137.
OMAiKWVRRGS.
OrthoDBiEOG6CGCKC.

Family and domain databases

Gene3Di3.40.710.10. 1 hit.
InterProiIPR012338. Beta-lactam/transpept-like.
IPR001264. Glyco_trans_51.
IPR023346. Lysozyme-like_dom.
IPR001460. PCN-bd_Tpept.
[Graphical view]
PfamiPF00912. Transgly. 1 hit.
PF00905. Transpeptidase. 1 hit.
[Graphical view]
SUPFAMiSSF53955. SSF53955. 1 hit.
SSF56601. SSF56601. 2 hits.

Sequencei

Sequence statusi: Complete.

A0R7G2-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MPPDDRLTAV LPPVRDGDIA PPIDVVRAAL EGSPPPKPPP PPPPGRGGGG
60 70 80 90 100
PSGPGGPSGP GRQFRINWKW VRRGSAIAVA VMVLLPLITF GMAYMIVDVP
110 120 130 140 150
EPGDIRTPQV STILASDGSE IARIVPPEGN RVDVKIDQIP VHVRDAVMAA
160 170 180 190 200
EDRDFYSNPG FSWTGFLRAI KNNLFGGGGL QGGSTITQQY VKNALVGDER
210 220 230 240 250
SGIGGLIRKA KELVISTKMS GEWSKDAVLQ AYLNIIYFGR GAYGISAASK
260 270 280 290 300
AYFDKPVEQL DVAEGALLAA LIQRPSTLDP AVDPEGAADR WNWVLDGMVD
310 320 330 340 350
IGALSQADRD AQVFPPTVPP DYAFQQNQTT GPNGLIERQV TNELLDLFDI
360 370 380 390 400
NEQTLNTEGL QITTTIDPKA QEAAVDAVDK YLEGQDPDMR AAVVSIDPRT
410 420 430 440 450
GGIKAYYGGS DANGFDFAQA GLPTGSSFKV FALVAALQQG IGLGYQIDSG
460 470 480 490 500
PLEVNGIKIG NVEGEGCGTC SIAEALKRSL NTSYYRLMLK LENGPADVAE
510 520 530 540 550
AAHDAGVAES FPGVEHTLSE DGKGGPPNNG VVLGQYQSRV LDMASAYATL
560 570 580 590 600
AASGVYHKPH FVEKVVNSSG QVLFDASKED NGEERIDKAV ADNVTSAMQP
610 620 630 640 650
IAGWSRGHNL AGGRPSAAKT GTVQLGDTGD NRDAWMVGYT PSLSTAVWVG
660 670 680 690 700
TTEGVKPLVN KWGSPIYGSG LPSDIWKATM DGALEGTDVE SFPKPTEIGG
710 720 730 740 750
YAGVPQAPAA PPPSAGPPTD PGQPSVTVIQ PTIEVAPGIT IPIGPPTTVP
760 770 780
VGPPPGAPGA PVGPGAPEVP VAPGAPGVPG APPPP
Length:785
Mass (Da):81,567
Last modified:January 9, 2007 - v1
Checksum:iA69C687EC368DF04
GO

Sequence cautioni

The sequence AFP43142.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000480 Genomic DNA. Translation: ABK70639.1.
CP001663 Genomic DNA. Translation: AFP43142.1. Different initiation.
RefSeqiYP_006571437.1. NC_018289.1.
YP_891100.1. NC_008596.1.

Genome annotation databases

EnsemblBacteriaiABK70639; ABK70639; MSMEG_6900.
AFP43142; AFP43142; MSMEI_6716.
GeneIDi4536904.
KEGGimsg:MSMEI_6716.
msm:MSMEG_6900.
PATRICi18086018. VBIMycSme59918_6721.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000480 Genomic DNA. Translation: ABK70639.1 .
CP001663 Genomic DNA. Translation: AFP43142.1 . Different initiation.
RefSeqi YP_006571437.1. NC_018289.1.
YP_891100.1. NC_008596.1.

3D structure databases

ProteinModelPortali A0R7G2.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 246196.MSMEG_6900.

Protein family/group databases

CAZyi GT51. Glycosyltransferase Family 51.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ABK70639 ; ABK70639 ; MSMEG_6900 .
AFP43142 ; AFP43142 ; MSMEI_6716 .
GeneIDi 4536904.
KEGGi msg:MSMEI_6716.
msm:MSMEG_6900.
PATRICi 18086018. VBIMycSme59918_6721.

Phylogenomic databases

eggNOGi COG0744.
HOGENOMi HOG000041137.
OMAi KWVRRGS.
OrthoDBi EOG6CGCKC.

Enzyme and pathway databases

UniPathwayi UPA00219 .
BioCyci MSME246196:GJ4Y-6899-MONOMER.

Family and domain databases

Gene3Di 3.40.710.10. 1 hit.
InterProi IPR012338. Beta-lactam/transpept-like.
IPR001264. Glyco_trans_51.
IPR023346. Lysozyme-like_dom.
IPR001460. PCN-bd_Tpept.
[Graphical view ]
Pfami PF00912. Transgly. 1 hit.
PF00905. Transpeptidase. 1 hit.
[Graphical view ]
SUPFAMi SSF53955. SSF53955. 1 hit.
SSF56601. SSF56601. 2 hits.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. Fleischmann R.D., Dodson R.J., Haft D.H., Merkel J.S., Nelson W.C., Fraser C.M.
    Submitted (OCT-2006) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 700084 / mc(2)155.
  2. "Interrupted coding sequences in Mycobacterium smegmatis: authentic mutations or sequencing errors?"
    Deshayes C., Perrodou E., Gallien S., Euphrasie D., Schaeffer C., Van-Dorsselaer A., Poch O., Lecompte O., Reyrat J.-M.
    Genome Biol. 8:R20.1-R20.9(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 700084 / mc(2)155.
  3. "Ortho-proteogenomics: multiple proteomes investigation through orthology and a new MS-based protocol."
    Gallien S., Perrodou E., Carapito C., Deshayes C., Reyrat J.-M., Van Dorsselaer A., Poch O., Schaeffer C., Lecompte O.
    Genome Res. 19:128-135(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 700084 / mc(2)155.
  4. "Interaction and modulation of two antagonistic cell wall enzymes of mycobacteria."
    Hett E.C., Chao M.C., Rubin E.J.
    PLoS Pathog. 6:E1001020-E1001020(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: DISRUPTION PHENOTYPE.
    Strain: ATCC 700084 / mc(2)155.

Entry informationi

Entry nameiPBP1A_MYCS2
AccessioniPrimary (citable) accession number: A0R7G2
Secondary accession number(s): I7GBX5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 6, 2013
Last sequence update: January 9, 2007
Last modified: October 29, 2014
This is version 56 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways

External Data

Dasty 3