Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Penicillin-binding protein 1A

Gene

ponA1

Organism
Mycobacterium smegmatis (strain ATCC 700084 / mc(2)155)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Cell wall formation. Synthesis of cross-linked peptidoglycan from the lipid intermediates. The enzyme has a penicillin-insensitive transglycosylase N-terminal domain (formation of linear glycan strands) and a penicillin-sensitive transpeptidase C-terminal domain (cross-linking of the peptide subunits) (By similarity).By similarity

Catalytic activityi

(GlcNAc-(1->4)-Mur2Ac(oyl-L-Ala-gamma-D-Glu-L-Lys-D-Ala-D-Ala))(n)-diphosphoundecaprenol + GlcNAc-(1->4)-Mur2Ac(oyl-L-Ala-gamma-D-Glu-L-Lys-D-Ala-D-Ala)-diphosphoundecaprenol = (GlcNAc-(1->4)-Mur2Ac(oyl-L-Ala-gamma-D-Glu-L-Lys-D-Ala-D-Ala))(n+1)-diphosphoundecaprenol + undecaprenyl diphosphate.By similarity
Preferential cleavage: (Ac)2-L-Lys-D-Ala-|-D-Ala. Also transpeptidation of peptidyl-alanyl moieties that are N-acyl substituents of D-alanine.By similarity

Pathwayi: peptidoglycan biosynthesis

This protein is involved in the pathway peptidoglycan biosynthesis, which is part of Cell wall biogenesis.
View all proteins of this organism that are known to be involved in the pathway peptidoglycan biosynthesis and in Cell wall biogenesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei151Proton donor; for transglycosylase activityBy similarity1
Active sitei426Acyl-ester intermediate; for transpeptidase activityBy similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionCarboxypeptidase, Glycosyltransferase, Hydrolase, Multifunctional enzyme, Protease, Transferase

Enzyme and pathway databases

BioCyciMSME246196:G1H7P-6863-MONOMER
UniPathwayiUPA00219

Protein family/group databases

CAZyiGT51 Glycosyltransferase Family 51

Names & Taxonomyi

Protein namesi
Recommended name:
Penicillin-binding protein 1A
Including the following 2 domains:
Penicillin-insensitive transglycosylase (EC:2.4.1.129By similarity)
Alternative name(s):
Peptidoglycan TGase
Penicillin-sensitive transpeptidase (EC:3.4.16.4By similarity)
Alternative name(s):
DD-transpeptidase
Gene namesi
Name:ponA1
Ordered Locus Names:MSMEG_6900, MSMEI_6716
OrganismiMycobacterium smegmatis (strain ATCC 700084 / mc(2)155)
Taxonomic identifieri246196 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaCorynebacterialesMycobacteriaceaeMycobacteriumMycobacterium fortuitum complex
Proteomesi
  • UP000006158 Componenti: Chromosome
  • UP000000757 Componenti: Chromosome

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transmembranei77 – 97HelicalSequence analysisAdd BLAST21

GO - Cellular componenti

Keywords - Cellular componenti

Cell membrane, Membrane

Pathology & Biotechi

Disruption phenotypei

Probably essential. In a depletion mutant growth rate is dramatically impaired, cells are short with bulbous poles and/or rounded internal regions.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00004211251 – 785Penicillin-binding protein 1AAdd BLAST785

Interactioni

Protein-protein interaction databases

STRINGi246196.MSMEG_6900

Structurei

3D structure databases

ProteinModelPortaliA0R7G2
SMRiA0R7G2
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni118 – 299TransglycosylaseAdd BLAST182
Regioni392 – 662TranspeptidaseAdd BLAST271

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi2 – 60Pro-richAdd BLAST59
Compositional biasi705 – 785Pro-richAdd BLAST81

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG4105BZ4 Bacteria
COG0744 LUCA
HOGENOMiHOG000041137
OMAiLAQMAMI
OrthoDBiPOG091H01NC

Family and domain databases

Gene3Di1.10.3810.10, 1 hit
InterProiView protein in InterPro
IPR012338 Beta-lactam/transpept-like
IPR001264 Glyco_trans_51
IPR023346 Lysozyme-like_dom_sf
IPR036950 PBP_transglycosylase
IPR001460 PCN-bd_Tpept
PfamiView protein in Pfam
PF00912 Transgly, 1 hit
PF00905 Transpeptidase, 1 hit
SUPFAMiSSF53955 SSF53955, 1 hit
SSF56601 SSF56601, 2 hits

Sequencei

Sequence statusi: Complete.

A0R7G2-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPPDDRLTAV LPPVRDGDIA PPIDVVRAAL EGSPPPKPPP PPPPGRGGGG
60 70 80 90 100
PSGPGGPSGP GRQFRINWKW VRRGSAIAVA VMVLLPLITF GMAYMIVDVP
110 120 130 140 150
EPGDIRTPQV STILASDGSE IARIVPPEGN RVDVKIDQIP VHVRDAVMAA
160 170 180 190 200
EDRDFYSNPG FSWTGFLRAI KNNLFGGGGL QGGSTITQQY VKNALVGDER
210 220 230 240 250
SGIGGLIRKA KELVISTKMS GEWSKDAVLQ AYLNIIYFGR GAYGISAASK
260 270 280 290 300
AYFDKPVEQL DVAEGALLAA LIQRPSTLDP AVDPEGAADR WNWVLDGMVD
310 320 330 340 350
IGALSQADRD AQVFPPTVPP DYAFQQNQTT GPNGLIERQV TNELLDLFDI
360 370 380 390 400
NEQTLNTEGL QITTTIDPKA QEAAVDAVDK YLEGQDPDMR AAVVSIDPRT
410 420 430 440 450
GGIKAYYGGS DANGFDFAQA GLPTGSSFKV FALVAALQQG IGLGYQIDSG
460 470 480 490 500
PLEVNGIKIG NVEGEGCGTC SIAEALKRSL NTSYYRLMLK LENGPADVAE
510 520 530 540 550
AAHDAGVAES FPGVEHTLSE DGKGGPPNNG VVLGQYQSRV LDMASAYATL
560 570 580 590 600
AASGVYHKPH FVEKVVNSSG QVLFDASKED NGEERIDKAV ADNVTSAMQP
610 620 630 640 650
IAGWSRGHNL AGGRPSAAKT GTVQLGDTGD NRDAWMVGYT PSLSTAVWVG
660 670 680 690 700
TTEGVKPLVN KWGSPIYGSG LPSDIWKATM DGALEGTDVE SFPKPTEIGG
710 720 730 740 750
YAGVPQAPAA PPPSAGPPTD PGQPSVTVIQ PTIEVAPGIT IPIGPPTTVP
760 770 780
VGPPPGAPGA PVGPGAPEVP VAPGAPGVPG APPPP
Length:785
Mass (Da):81,567
Last modified:January 9, 2007 - v1
Checksum:iA69C687EC368DF04
GO

Sequence cautioni

The sequence AFP43142 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000480 Genomic DNA Translation: ABK70639.1
CP001663 Genomic DNA Translation: AFP43142.1 Different initiation.
RefSeqiWP_011731616.1, NZ_CP009494.1
YP_891100.1, NC_008596.1

Genome annotation databases

EnsemblBacteriaiABK70639; ABK70639; MSMEG_6900
AFP43142; AFP43142; MSMEI_6716
GeneIDi4536904
KEGGimsg:MSMEI_6716
msm:MSMEG_6900
PATRICifig|246196.19.peg.6721

Similar proteinsi

Entry informationi

Entry nameiPBP1A_MYCS2
AccessioniPrimary (citable) accession number: A0R7G2
Secondary accession number(s): I7GBX5
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 6, 2013
Last sequence update: January 9, 2007
Last modified: May 23, 2018
This is version 74 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Cookie policy

We would like to use anonymized google analytics cookies to gather statistics on how uniprot.org is used in aggregate. Learn more

UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health