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Protein

D-erythrulose-4-phosphate isomerase 2

Gene

derI2

Organism
Mycobacterium smegmatis (strain ATCC 700084 / mc(2)155)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the isomerization of D-erythrulose-4P to D-erythrose-4P. Involved in the degradation pathways of L-threitol, D-threitol and erythritol, that allow M.smegmatis to grow on these compounds as the sole carbon source.1 Publication

Catalytic activityi

D-erythrulose 4-phosphate = D-erythrose 4-phosphate.1 Publication

Pathwayi: erythritol degradation

This protein is involved in the pathway erythritol degradation, which is part of Carbohydrate metabolism.1 Publication
View all proteins of this organism that are known to be involved in the pathway erythritol degradation and in Carbohydrate metabolism.

Pathwayi: D-threitol degradation

This protein is involved in the pathway D-threitol degradation, which is part of Carbohydrate metabolism.1 Publication
View all proteins of this organism that are known to be involved in the pathway D-threitol degradation and in Carbohydrate metabolism.

Pathwayi: L-threitol degradation

This protein is involved in the pathway L-threitol degradation, which is part of Carbohydrate metabolism.1 Publication
View all proteins of this organism that are known to be involved in the pathway L-threitol degradation and in Carbohydrate metabolism.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei70 – 701Proton acceptorBy similarity

GO - Molecular functioni

GO - Biological processi

  • carbohydrate catabolic process Source: UniProtKB
  • carbohydrate utilization Source: UniProtKB
  • cellular response to carbohydrate stimulus Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Isomerase

Keywords - Biological processi

Carbohydrate metabolism

Enzyme and pathway databases

BioCyciMSME246196:GJ4Y-6786-MONOMER.
UniPathwayiUPA01065.
UPA01066.
UPA01067.

Names & Taxonomyi

Protein namesi
Recommended name:
D-erythrulose-4-phosphate isomerase 21 Publication (EC:5.3.1.-1 Publication)
Gene namesi
Name:derI21 Publication
Ordered Locus Names:MSMEG_6787Imported, MSMEI_6604Imported
OrganismiMycobacterium smegmatis (strain ATCC 700084 / mc(2)155)
Taxonomic identifieri246196 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaCorynebacterialesMycobacteriaceaeMycobacterium
Proteomesi
  • UP000006158 Componenti: Chromosome
  • UP000000757 Componenti: Chromosome

Pathology & Biotechi

Disruption phenotypei

Cells lacking this gene are totally unable to grow on L-threitol. Cells lacking both derI1 and derI2 are totally unable to grow on D-threitol or on erythritol.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 152152D-erythrulose-4-phosphate isomerase 2PRO_0000435518Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi246196.MSMEG_6787.

Family & Domainsi

Sequence similaritiesi

Belongs to the LacAB/RpiB family.Curated

Phylogenomic databases

eggNOGiENOG4107YVI. Bacteria.
COG0698. LUCA.
HOGENOMiHOG000226301.
KOiK01808.
OMAiARYSKEH.
OrthoDBiEOG679TJ4.

Family and domain databases

Gene3Di3.40.1400.10. 1 hit.
InterProiIPR011860. Rib-5-P_Isoase.
IPR003500. RpiB_LacA_LacB.
[Graphical view]
PANTHERiPTHR30345. PTHR30345. 1 hit.
PfamiPF02502. LacAB_rpiB. 1 hit.
[Graphical view]
PIRSFiPIRSF005384. RpiB_LacA_B. 1 hit.
SUPFAMiSSF89623. SSF89623. 1 hit.
TIGRFAMsiTIGR02133. RPI_actino. 1 hit.
TIGR00689. rpiB_lacA_lacB. 1 hit.

Sequencei

Sequence statusi: Complete.

A0R757-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MALKIVIGGD NAGFNYKEAL RKDLEADDRV ASVEDVGVGG VDDTTSYPNV
60 70 80 90 100
AVAAAEKVAR GEADRALLIC GTGLGVAIAA NKVKGIRAVT AHDVYSVQRS
110 120 130 140 150
VLSNNAQVLC MGERVVGLEL ARALVKEWLG LEFDPQSASA AKVNDICAYE

GA
Length:152
Mass (Da):15,849
Last modified:January 9, 2007 - v1
Checksum:i9DAB1894937EC464
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000480 Genomic DNA. Translation: ABK71442.1.
CP001663 Genomic DNA. Translation: AFP43030.1.
RefSeqiWP_011731539.1. NZ_CP009494.1.
YP_890995.1. NC_008596.1.

Genome annotation databases

EnsemblBacteriaiABK71442; ABK71442; MSMEG_6787.
AFP43030; AFP43030; MSMEI_6604.
GeneIDi4531552.
KEGGimsb:LJ00_33535.
msg:MSMEI_6604.
msm:MSMEG_6787.
PATRICi18085790. VBIMycSme59918_6607.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000480 Genomic DNA. Translation: ABK71442.1.
CP001663 Genomic DNA. Translation: AFP43030.1.
RefSeqiWP_011731539.1. NZ_CP009494.1.
YP_890995.1. NC_008596.1.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi246196.MSMEG_6787.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABK71442; ABK71442; MSMEG_6787.
AFP43030; AFP43030; MSMEI_6604.
GeneIDi4531552.
KEGGimsb:LJ00_33535.
msg:MSMEI_6604.
msm:MSMEG_6787.
PATRICi18085790. VBIMycSme59918_6607.

Phylogenomic databases

eggNOGiENOG4107YVI. Bacteria.
COG0698. LUCA.
HOGENOMiHOG000226301.
KOiK01808.
OMAiARYSKEH.
OrthoDBiEOG679TJ4.

Enzyme and pathway databases

UniPathwayiUPA01065.
UPA01066.
UPA01067.
BioCyciMSME246196:GJ4Y-6786-MONOMER.

Family and domain databases

Gene3Di3.40.1400.10. 1 hit.
InterProiIPR011860. Rib-5-P_Isoase.
IPR003500. RpiB_LacA_LacB.
[Graphical view]
PANTHERiPTHR30345. PTHR30345. 1 hit.
PfamiPF02502. LacAB_rpiB. 1 hit.
[Graphical view]
PIRSFiPIRSF005384. RpiB_LacA_B. 1 hit.
SUPFAMiSSF89623. SSF89623. 1 hit.
TIGRFAMsiTIGR02133. RPI_actino. 1 hit.
TIGR00689. rpiB_lacA_lacB. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. Fleischmann R.D., Dodson R.J., Haft D.H., Merkel J.S., Nelson W.C., Fraser C.M.
    Submitted (OCT-2006) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 700084 / mc(2)155.
  2. "Interrupted coding sequences in Mycobacterium smegmatis: authentic mutations or sequencing errors?"
    Deshayes C., Perrodou E., Gallien S., Euphrasie D., Schaeffer C., Van-Dorsselaer A., Poch O., Lecompte O., Reyrat J.-M.
    Genome Biol. 8:R20.1-R20.9(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 700084 / mc(2)155.
  3. "Ortho-proteogenomics: multiple proteomes investigation through orthology and a new MS-based protocol."
    Gallien S., Perrodou E., Carapito C., Deshayes C., Reyrat J.-M., Van Dorsselaer A., Poch O., Schaeffer C., Lecompte O.
    Genome Res. 19:128-135(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 700084 / mc(2)155.
  4. "A general strategy for the discovery of metabolic pathways: D-threitol, L-threitol, and erythritol utilization in Mycobacterium smegmatis."
    Huang H., Carter M.S., Vetting M.W., Al-Obaidi N., Patskovsky Y., Almo S.C., Gerlt J.A.
    J. Am. Chem. Soc. 137:14570-14573(2015) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, CATALYTIC ACTIVITY, DISRUPTION PHENOTYPE, PATHWAY.
    Strain: ATCC 700084 / mc(2)155.

Entry informationi

Entry nameiDERI2_MYCS2
AccessioniPrimary (citable) accession number: A0R757
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 17, 2016
Last sequence update: January 9, 2007
Last modified: May 11, 2016
This is version 76 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.