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Protein

L-erythrulose-1-phosphate isomerase

Gene

lerI

Organism
Mycobacterium smegmatis (strain ATCC 700084 / mc(2)155)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalyzes the isomerization of L-erythrulose-1P to D-erythrulose-4P. Involved in the degradation pathway of L-threitol, that allows M.smegmatis to grow on this compound as the sole carbon source.1 Publication1 Publication

Catalytic activityi

L-erythrulose 1-phosphate = D-erythrulose 4-phosphate.1 Publication1 Publication

Pathwayi: L-threitol degradation

This protein is involved in the pathway L-threitol degradation, which is part of Carbohydrate metabolism.1 Publication
View all proteins of this organism that are known to be involved in the pathway L-threitol degradation and in Carbohydrate metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei106ElectrophileBy similarity1
Active sitei178Proton acceptorBy similarity1

GO - Molecular functioni

GO - Biological processi

  • carbohydrate catabolic process Source: UniProtKB
  • carbohydrate utilization Source: UniProtKB

Keywordsi

Molecular functionIsomerase
Biological processCarbohydrate metabolism

Enzyme and pathway databases

BioCyciMetaCyc:MONOMER-19894.
MSME246196:G1H7P-6750-MONOMER.
UniPathwayiUPA01067.

Names & Taxonomyi

Protein namesi
Recommended name:
L-erythrulose-1-phosphate isomerase1 Publication (EC:5.3.1.331 Publication1 Publication)
Gene namesi
Name:lerI1 Publication
Synonyms:tpiAImported
Ordered Locus Names:MSMEG_6785Imported, MSMEI_6603Imported
OrganismiMycobacterium smegmatis (strain ATCC 700084 / mc(2)155)
Taxonomic identifieri246196 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaCorynebacterialesMycobacteriaceaeMycobacterium
Proteomesi
  • UP000006158 Componenti: Chromosome
  • UP000000757 Componenti: Chromosome

Pathology & Biotechi

Disruption phenotypei

Cells lacking this gene are totally unable to grow on L-threitol.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00004355191 – 263L-erythrulose-1-phosphate isomeraseAdd BLAST263

Interactioni

Protein-protein interaction databases

STRINGi246196.MSMEG_6785.

Structurei

3D structure databases

ProteinModelPortaliA0R756.
SMRiA0R756.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the triosephosphate isomerase family.Curated

Phylogenomic databases

eggNOGiENOG4108MKX. Bacteria.
COG0149. LUCA.
HOGENOMiHOG000226412.
KOiK20905.
OMAiEPQWAIG.
OrthoDBiPOG091H02GL.

Family and domain databases

CDDicd00311. TIM. 1 hit.
Gene3Di3.20.20.70. 1 hit.
InterProiView protein in InterPro
IPR013785. Aldolase_TIM.
IPR035990. TIM_sf.
IPR000652. Triosephosphate_isomerase.
IPR020861. Triosephosphate_isomerase_AS.
PANTHERiPTHR21139. PTHR21139. 1 hit.
PfamiView protein in Pfam
PF00121. TIM. 1 hit.
SUPFAMiSSF51351. SSF51351. 1 hit.
PROSITEiView protein in PROSITE
PS00171. TIM_1. 1 hit.
PS51440. TIM_2. 1 hit.

Sequencei

Sequence statusi: Complete.

A0R756-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPDARALGAA QLWIGTSWKM NKGLAESRGY ARELAEYVAA KPPAGVQPFI
60 70 80 90 100
IPSFTALTTV RDALGDDSPV LLGVQNAHWE DHGAWTGEVS VAQAKDAGAQ
110 120 130 140 150
IVEIGHSERR EHFGETVETT RLKVAAALHH GLVPLLCIGE SAENKQAGES
160 170 180 190 200
SRFILEQAAG ALEGLTDEHL ARVLIAYEPI WAIGENGRPA TVEELRQPFD
210 220 230 240 250
DLAREYGCRT MGLLYGGSVN TDNAEDLLGI DHVTGLFIGR AAWQLPGYVR
260
ILEMAAAHPK AKA
Length:263
Mass (Da):28,295
Last modified:January 9, 2007 - v1
Checksum:i966EA3BC94FC6BF8
GO

Sequence cautioni

The sequence AFP43029 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000480 Genomic DNA. Translation: ABK75255.1.
CP001663 Genomic DNA. Translation: AFP43029.1. Different initiation.
RefSeqiWP_011731538.1. NZ_CP009494.1.
YP_890994.1. NC_008596.1.

Genome annotation databases

EnsemblBacteriaiABK75255; ABK75255; MSMEG_6785.
AFP43029; AFP43029; MSMEI_6603.
GeneIDi4533146.
KEGGimsb:LJ00_33530.
msg:MSMEI_6603.
msm:MSMEG_6785.
PATRICifig|246196.19.peg.6606.

Similar proteinsi

Entry informationi

Entry nameiLERI_MYCS2
AccessioniPrimary (citable) accession number: A0R756
Secondary accession number(s): I7GGP3
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 17, 2016
Last sequence update: January 9, 2007
Last modified: February 28, 2018
This is version 85 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Caution

The substrate of the reaction was originally identified as L-erythrulose 4-phosphate (PubMed:26560079). It was then corrected to L-erythrulose 1-phosphate by the same group (PubMed:26978037).2 Publications

Keywords - Technical termi

Complete proteome, Reference proteome