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A0R6Q9 (A0R6Q9_MYCS2) Unreviewed, UniProtKB/TrEMBL

Last modified December 14, 2011. Version 46. Feed History...

Clusters with 100%, 90%, 50% identity | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase HAMAP MF_00172
EC=2.1.1.14 HAMAP MF_00172
Alternative name(s):
Cobalamin-independent methionine synthase HAMAP MF_00172
Methionine synthase, vitamin-B12 independent isozyme HAMAP MF_00172
Gene names
Name:metE HAMAP MF_00172
Ordered Locus Names:MSMEG_6638
OrganismMycobacterium smegmatis (strain ATCC 700084 / mc(2)155) EMBL ABK69957.1
Taxonomic identifier246196 [NCBI]
Taxonomic lineageBacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Corynebacterineae › Mycobacteriaceae › Mycobacterium

Protein attributes

Sequence length771 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the transfer of a methyl group from 5-methyltetrahydrofolate to homocysteine resulting in methionine formation By similarity. HAMAP MF_00172

Catalytic activity

5-methyltetrahydropteroyltri-L-glutamate + L-homocysteine = tetrahydropteroyltri-L-glutamate + L-methionine. HAMAP MF_00172

Cofactor

Binds 1 zinc ion per subunit By similarity. HAMAP MF_00172

Pathway

Amino-acid biosynthesis; L-methionine biosynthesis via de novo pathway; L-methionine from L-homocysteine (MetE route): step 1/1. HAMAP MF_00172

Sequence similarities

Belongs to the vitamin-B12 independent methionine synthase family. HAMAP MF_00172

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Sites

Metal binding6581Zinc By similarity HAMAP MF_00172
Metal binding6601Zinc By similarity HAMAP MF_00172
Metal binding7431Zinc By similarity HAMAP MF_00172

Sequences

Sequence LengthMass (Da)Tools
A0R6Q9 [UniParc].

Last modified January 9, 2007. Version 1.
Checksum: EC8AFE5F58E8628C

FASTA77183,059
        10         20         30         40         50         60 
MAFRRTEEDV LMTATPFTAT ILGSPRIGPN RELKRAVEKY WAGNLDRSGL ESVAAKIRRD 

        70         80         90        100        110        120 
TWESLTAAGL DSVPVNTFSY YDHVLDTAVL VGALPPRVAG VADDLDRYFA AARGGTYDGT 

       130        140        150        160        170        180 
DIAPLEMTKW FDTNYHYIVP ELGPDTSFAL HPAKVIEELR EARALGIPAR PVVVGPITFL 

       190        200        210        220        230        240 
ALSKAVGGAV APISRLGELV SVYGQLLELL ADEGVQWVQI DEPVLVTDIL DNAAELAGQT 

       250        260        270        280        290        300 
YKVLGGLAKR PALFVASYFG ELTDALPALA RTPVEAIGVD LVAGTAAAVA AVPELTTKTV 

       310        320        330        340        350        360 
VAGVVDGRNI WRTDLQSALG TLASLLGSVG NLAVSTSCST LHVPYTLDAE SELDPALRSW 

       370        380        390        400        410        420 
LAFGFEKVTE VVALARGLTQ GREAIAGEIA ASNDAIAARK SDPRLNNDHV RERLSSILAS 

       430        440        450        460        470        480 
GAQRGPAARR REVQQDRLGL PPLPTTTIGS YPQTSAIRVA RAELRNGTID TAEYERRMKH 

       490        500        510        520        530        540 
EIAGVVKLQE DLGLDVLVHG EPERNDMVQY FAEQLTGFFA TENGWVQSYG SRCVRPPILY 

       550        560        570        580        590        600 
GDVARPEPMT VSWAQYAQSL TDKPMKGMLT GPVTILAWSF VRDDQPLADT AAQVALAIRD 

       610        620        630        640        650        660 
ETVDLQAAGI VIIQVDEPAL RELLPLRSKD QAAYLKWAVE AFRLATSGVE DSTQIHTHLC 

       670        680        690        700        710        720 
YSEFGEVIGA IADLDADVTS IEAARSHMEV LDDLNAAGFG NGVGPGVYDI HSPRVPTTEE 

       730        740        750        760        770 
MATALRNALK AVPAERLWVN PDCGLKTRKT DEVTESLRNM VDAAREARAD A

« Hide

References

[1]Fleischmann R.D., Dodson R.J., Haft D.H., Merkel J.S., Nelson W.C., Fraser C.M.
Submitted (OCT-2006) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 700084 / mc(2)155.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		CP000480 Genomic DNA. Translation: ABK69957.1.
RefSeqYP_890847.1. NC_008596.1.

3D structure databases

ProteinModelPortalA0R6Q9.
ModBaseSearch...

Protein-protein interaction databases

STRINGA0R6Q9.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaEBMYCT00000043544; EBMYCP00000041881; EBMYCG00000043539.
GeneID4533405.
GenomeReviewsGene locus MSMEG_6638 in contig CP000480_GR.
KEGGmsm:MSMEG_6638.
NMPDRfig|246196.1.peg.6600.
PATRIC18085501. VBIMycSme59918_6463.
TIGRMSMEG_6638.

Phylogenomic databases

eggNOGCOG0620.
GeneTreeEBGT00050000017657.
HOGENOMHBG287495.
OMARNIWRAN.
PhylomeDBA0R6Q9.
ProtClustDBPRK05222.

Family and domain databases

HAMAPMF_00172. Meth_synth.
[Tree]
InterProIPR013215. Cbl-indep_Met_Synth_N.
IPR006276. Cobalamin-indep_Met_synthase.
IPR002629. Methionine_synth.
[Graphical view]
KOK00549.
PfamPF08267. Meth_synt_1. 1 hit.
PF01717. Meth_synt_2. 1 hit.
[Graphical view]
PIRSFPIRSF000382. MeTrfase_B12_ind. 1 hit.
TIGRFAMsTIGR01371. Met_syn_B12ind. 1 hit.
ProtoNetSearch...

Entry information

Entry nameA0R6Q9_MYCS2
AccessionPrimary (citable) accession number: A0R6Q9
Entry history
Integrated into UniProtKB/TrEMBL: January 9, 2007
Last sequence update: January 9, 2007
Last modified: December 14, 2011
This is version 46 of the entry and version 1 of the sequence. [Complete history]
Entry statusUnreviewed (UniProtKB/TrEMBL)
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info