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A0R666 (ETHR_MYCS2) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 55. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
HTH-type transcriptional regulator EthR
Gene names
Name:ethR
Ordered Locus Names:MSMEG_6441, MSMEI_6273
OrganismMycobacterium smegmatis (strain ATCC 700084 / mc(2)155) [Reference proteome] [HAMAP]
Taxonomic identifier246196 [NCBI]
Taxonomic lineageBacteriaActinobacteriaActinobacteridaeActinomycetalesCorynebacterineaeMycobacteriaceaeMycobacterium

Protein attributes

Sequence length217 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Involved in the repression of teh monooxygenase EthA which is responsible of the formation of the active metabolite of ethionamide (ETH) Probable. Ref.4

Subunit structure

Homodimer By similarity.

Sequence similarities

Contains 1 HTH tetR-type DNA-binding domain.

Ontologies

Keywords
   Biological processTranscription
Transcription regulation
   LigandDNA-binding
   Molecular functionRepressor
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processnegative regulation of transcription, DNA-templated

Inferred from mutant phenotype Ref.4. Source: UniProtKB

transcription, DNA-templated

Inferred from electronic annotation. Source: UniProtKB-KW

   Molecular_functionDNA binding

Inferred from electronic annotation. Source: UniProtKB-KW

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 217217HTH-type transcriptional regulator EthR
PRO_0000398580

Regions

Domain21 – 8161HTH tetR-type
DNA binding44 – 6320H-T-H motif By similarity

Sites

Site1731Inhibitor-binding By similarity
Site1761Inhibitor-binding By similarity

Sequences

Sequence LengthMass (Da)Tools
A0R666 [UniParc].

Last modified January 9, 2007. Version 1.
Checksum: 37BDD17023464AC5

FASTA21723,512
        10         20         30         40         50         60 
MTTASQTRTP RGRRSARPSG DDREAAILAT AQRLLETKKF AEISVDDLAK GAGISRPTFY 

        70         80         90        100        110        120 
FYFPSKEAVL LSLIDPLIKR ADSGFDNAVE SMPADPQRAI RRGIEIFFNS FGSHPATARA 

       130        140        150        160        170        180 
GTEALKSSPE FKEFWSGLMQ KWIAATAALI TAERERGAAP DTIPALDLAT SLNLMNERTM 

       190        200        210 
MAALADEQPG VAPEKVVATL THIWLNSIYG TLPVGTA 

« Hide

References

« Hide 'large scale' references
[1]Fleischmann R.D., Dodson R.J., Haft D.H., Merkel J.S., Nelson W.C., Fraser C.M.
Submitted (OCT-2006) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 700084 / mc(2)155.
[2]"Interrupted coding sequences in Mycobacterium smegmatis: authentic mutations or sequencing errors?"
Deshayes C., Perrodou E., Gallien S., Euphrasie D., Schaeffer C., Van-Dorsselaer A., Poch O., Lecompte O., Reyrat J.-M.
Genome Biol. 8:R20.1-R20.9(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 700084 / mc(2)155.
[3]"Ortho-proteogenomics: multiple proteomes investigation through orthology and a new MS-based protocol."
Gallien S., Perrodou E., Carapito C., Deshayes C., Reyrat J.-M., Van Dorsselaer A., Poch O., Schaeffer C., Lecompte O.
Genome Res. 19:128-135(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 700084 / mc(2)155.
[4]"Activation of the pro-drug ethionamide is regulated in mycobacteria."
Baulard A.R., Betts J.C., Engohang-Ndong J., Quan S., McAdam R.A., Brennan P.J., Locht C., Besra G.S.
J. Biol. Chem. 275:28326-28331(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION AS A REPRESSOR ETHA.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP000480 Genomic DNA. Translation: ABK70466.1.
CP001663 Genomic DNA. Translation: AFP42699.1.
RefSeqYP_006570994.1. NC_018289.1.
YP_890654.1. NC_008596.1.

3D structure databases

ProteinModelPortalA0R666.
SMRA0R666. Positions 20-210.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING246196.MSMEG_6441.

Chemistry

ChEMBLCHEMBL2163179.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaABK70466; ABK70466; MSMEG_6441.
AFP42699; AFP42699; MSMEI_6273.
GeneID4535770.
KEGGmsg:MSMEI_6273.
msm:MSMEG_6441.
PATRIC18085107. VBIMycSme59918_6266.

Phylogenomic databases

eggNOGCOG1309.
HOGENOMHOG000054523.
OMAFMQKWID.
OrthoDBEOG67DPRF.

Enzyme and pathway databases

BioCycMSME246196:GJ4Y-6440-MONOMER.

Family and domain databases

Gene3D1.10.10.60. 1 hit.
1.10.357.10. 1 hit.
InterProIPR009057. Homeodomain-like.
IPR001647. HTH_TetR.
IPR015893. Tet_transcr_reg_TetR-like_C.
IPR011075. Tet_transcr_reg_TetR-rel_C.
[Graphical view]
PfamPF00440. TetR_N. 1 hit.
[Graphical view]
PRINTSPR00455. HTHTETR.
SUPFAMSSF46689. SSF46689. 1 hit.
SSF48498. SSF48498. 1 hit.
PROSITEPS50977. HTH_TETR_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameETHR_MYCS2
AccessionPrimary (citable) accession number: A0R666
Secondary accession number(s): I7GGF8
Entry history
Integrated into UniProtKB/Swiss-Prot: October 5, 2010
Last sequence update: January 9, 2007
Last modified: July 9, 2014
This is version 55 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families