A0R666 (ETHR_MYCS2) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
HTH-type transcriptional regulator EthR

Gene names

Name:	ethR
Ordered Locus Names:	MSMEG_6441, MSMEI_6273

Organism

Mycobacterium smegmatis (strain ATCC 700084 / mc(2)155) [Reference proteome] [HAMAP]

Taxonomic identifier

246196 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Actinobacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Corynebacterineae › Mycobacteriaceae › Mycobacterium › Mycobacterium smegmatis

Protein attributes

Sequence length	217 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Involved in the repression of teh monooxygenase EthA which is responsible of the formation of the active metabolite of ethionamide (ETH) Probable. Ref.4
Subunit structure	Homodimer By similarity.
Sequence similarities	Contains 1 HTH tetR-type DNA-binding domain.

Ontologies

Keywords
Biological process	Transcription Transcription regulation
Ligand	DNA-binding
Molecular function	Repressor
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	negative regulation of transcription, DNA-dependent Inferred from mutant phenotype Ref.4. Source: UniProtKB transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	DNA binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 217	217	HTH-type transcriptional regulator EthR		PRO_0000398580
Regions
	Domain	21 – 81	61	HTH tetR-type
	DNA binding	44 – 63	20	H-T-H motif By similarity
Sites
	Site	173	1	Inhibitor-binding By similarity
	Site	176	1	Inhibitor-binding By similarity

Sequences

	Sequence		Length	Mass (Da)
	A0R666 [UniParc]. Last modified January 9, 2007. Version 1. Checksum: 37BDD17023464AC5	FASTA	217	23,512
	10 20 30 40 50 60 MTTASQTRTP RGRRSARPSG DDREAAILAT AQRLLETKKF AEISVDDLAK GAGISRPTFY 70 80 90 100 110 120 FYFPSKEAVL LSLIDPLIKR ADSGFDNAVE SMPADPQRAI RRGIEIFFNS FGSHPATARA 130 140 150 160 170 180 GTEALKSSPE FKEFWSGLMQ KWIAATAALI TAERERGAAP DTIPALDLAT SLNLMNERTM 190 200 210 MAALADEQPG VAPEKVVATL THIWLNSIYG TLPVGTA

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	Fleischmann R.D., Dodson R.J., Haft D.H., Merkel J.S., Nelson W.C., Fraser C.M. Submitted (OCT-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 700084 / mc(2)155.
[2]	"Interrupted coding sequences in Mycobacterium smegmatis: authentic mutations or sequencing errors?" Deshayes C., Perrodou E., Gallien S., Euphrasie D., Schaeffer C., Van-Dorsselaer A., Poch O., Lecompte O., Reyrat J.-M. Genome Biol. 8:R20.1-R20.9(2007) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 700084 / mc(2)155.
[3]	"Ortho-proteogenomics: multiple proteomes investigation through orthology and a new MS-based protocol." Gallien S., Perrodou E., Carapito C., Deshayes C., Reyrat J.-M., Van Dorsselaer A., Poch O., Schaeffer C., Lecompte O. Genome Res. 19:128-135(2009) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 700084 / mc(2)155.
[4]	"Activation of the pro-drug ethionamide is regulated in mycobacteria." Baulard A.R., Betts J.C., Engohang-Ndong J., Quan S., McAdam R.A., Brennan P.J., Locht C., Besra G.S. J. Biol. Chem. 275:28326-28331(2000) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION AS A REPRESSOR ETHA.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000480 Genomic DNA. Translation: ABK70466.1. CP001663 Genomic DNA. Translation: AFP42699.1.
RefSeq	YP_006570994.1. NC_018289.1. YP_890654.1. NC_008596.1.
3D structure databases
ProteinModelPortal	A0R666.
SMR	A0R666. Positions 20-210.
ModBase	Search...
Protein-protein interaction databases
STRING	246196.MSMEG_6441.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	ABK70466; ABK70466; MSMEG_6441.
GeneID	13427603. 4535770.
KEGG	msg:MSMEI_6273. msm:MSMEG_6441.
PATRIC	18085107. VBIMycSme59918_6266.
Phylogenomic databases
eggNOG	COG1309.
HOGENOM	HOG000054523.
OMA	FMQKWID.
ProtClustDB	CLSK872258.
Enzyme and pathway databases
BioCyc	MSME246196:GJ4Y-6440-MONOMER.
Family and domain databases
Gene3D	1.10.10.60. 1 hit. 1.10.357.10. 1 hit.
InterPro	IPR009057. Homeodomain-like. IPR001647. HTH_TetR. IPR015893. Tet_transcr_reg_TetR-like_C. IPR011075. Tet_transcr_reg_TetR-rel_C. [Graphical view]
Pfam	PF00440. TetR_N. 1 hit. [Graphical view]
PRINTS	PR00455. HTHTETR.
SUPFAM	SSF46689. Homeodomain_like. 1 hit. SSF48498. TetR_like_C. 1 hit.
PROSITE	PS01081. HTH_TETR_1. False negative. PS50977. HTH_TETR_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ETHR_MYCS2

Accession

Primary (citable) accession number: A0R666
Secondary accession number(s): I7GGF8

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 5, 2010
Last sequence update:	January 9, 2007
Last modified:	May 1, 2013
This is version 48 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program