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Protein

FAD-containing monooxygenase EthA

Gene

ethA

Organism
Mycobacterium smegmatis (strain ATCC 700084 / mc(2)155)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Monooxygenase able to convert a wide range of ketones to the corresponding esters or lactones via a Baeyer-Villiger oxidation reaction. Can act on long-chain aliphatic ketones (2-hexanone to 2-dodecanone) and on aromatic ketones (phenylacetone and benzylacetone). Is also able to catalyze enantioselective sulfoxidation of methyl-p-tolylsulfide. In vivo, likely functions as a BVMO, but the exact nature of the physiological substrate(s) remains to be established.By similarity
Is responsible for the activation of several thiocarbamide-containing pro-drugs, such as ethionamide (ETH), isoxyl (ISO) and thiacetazone (TAC), into reactive species.By similarity

Catalytic activityi

Ethionamide + NADPH + O2 = ethionamide S-oxide + NADP+ + H2O.By similarity

Cofactori

FADBy similarityNote: Binds 1 FAD per subunit.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei15FADBy similarity1
Binding sitei36FADBy similarity1
Binding sitei56FADBy similarity1
Binding sitei104FAD; via amide nitrogen and carbonyl oxygenBy similarity1
Sitei292Transition state stabilizerBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi44 – 47FADBy similarity4
Nucleotide bindingi54 – 56NADPBy similarity3
Nucleotide bindingi183 – 189NADPBy similarity7
Nucleotide bindingi207 – 208NADPBy similarity2

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Monooxygenase, Oxidoreductase

Keywords - Ligandi

FAD, Flavoprotein, NADP

Names & Taxonomyi

Protein namesi
Recommended name:
FAD-containing monooxygenase EthA (EC:1.14.13.-By similarity)
Alternative name(s):
Baeyer-Villiger monooxygenase
Short name:
BVMO
Prodrug activator EtaA
Gene namesi
Name:ethA
Ordered Locus Names:MSMEG_6440, MSMEI_6272
OrganismiMycobacterium smegmatis (strain ATCC 700084 / mc(2)155)
Taxonomic identifieri246196 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaCorynebacterialesMycobacteriaceaeMycobacterium
Proteomesi
  • UP000006158 Componenti: Chromosome
  • UP000000757 Componenti: Chromosome

Subcellular locationi

  • Cell membrane By similarity

  • Note: Is most likely membrane-associated.By similarity

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003985831 – 492FAD-containing monooxygenase EthAAdd BLAST492

Expressioni

Inductioni

Repressed by the transcriptional regulator EthR.By similarity

Interactioni

Protein-protein interaction databases

STRINGi246196.MSMEG_6440.

Structurei

3D structure databases

ProteinModelPortaliA0R665.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the FAD-binding monooxygenase family.Curated

Phylogenomic databases

eggNOGiENOG4105CT3. Bacteria.
COG2072. LUCA.
HOGENOMiHOG000143616.
KOiK10215.
OMAiRRANIGG.
OrthoDBiPOG091H0APU.

Family and domain databases

Gene3Di3.50.50.60. 2 hits.
InterProiIPR023753. FAD/NAD-binding_dom.
IPR020946. Flavin_mOase-like.
[Graphical view]
PfamiPF00743. FMO-like. 1 hit.
[Graphical view]
SUPFAMiSSF51905. SSF51905. 1 hit.

Sequencei

Sequence statusi: Complete.

A0R665-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTEHFDVVIV GAGISGISTA WHLQDRCPTK SYVILERRAN IGGTWDLFKY
60 70 80 90 100
PGIRSDSDMF TLGFRFKPWT SAKSIADGPS IWNYINEAAQ ENGIDKHIRT
110 120 130 140 150
NHRVLGADWS DAENRWTITV EADGEQKQIT ASFLSVCSGY YNYDQGYSPE
160 170 180 190 200
FPGADDFAGQ IIHPQHWPED LDYAGKKIVV IGSGATAVTL IPSLVNGGAA
210 220 230 240 250
HVTMLQRSPT YIGSLPLVDP VAEKTNKYLP KNLAHFVNRW KAIAFSTAQY
260 270 280 290 300
QLARKFPNYM RKTLMTMAQR RLPEGFDVQK HFGPRYNPWD ERLCLAPNGD
310 320 330 340 350
LFKTIRAGKA DVVTDTIAKF TETGIKLTSG EELTADIIIT ATGLNMQLFG
360 370 380 390 400
GASLTRNGQE VDLTETMTYK GLMLSGVPNM AITFGYTNAS WTLKADLVSE
410 420 430 440 450
FICRVLNYMD DNGFDRVEPQ HPGDAVDALP FMDFNPGYFR RAMDSLPKSG
460 470 480 490
SRAPWRLKQN YFFDLRMIRY DKVDEESLHF TKHRAAVSAS SS
Length:492
Mass (Da):55,099
Last modified:January 9, 2007 - v1
Checksum:iA89FF49F0770A644
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000480 Genomic DNA. Translation: ABK75948.1.
CP001663 Genomic DNA. Translation: AFP42698.1.
RefSeqiWP_003897853.1. NZ_CP009494.1.
YP_890653.1. NC_008596.1.

Genome annotation databases

EnsemblBacteriaiABK75948; ABK75948; MSMEG_6440.
AFP42698; AFP42698; MSMEI_6272.
GeneIDi4537741.
KEGGimsb:LJ00_31840.
msg:MSMEI_6272.
msm:MSMEG_6440.
PATRICi18085105. VBIMycSme59918_6265.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000480 Genomic DNA. Translation: ABK75948.1.
CP001663 Genomic DNA. Translation: AFP42698.1.
RefSeqiWP_003897853.1. NZ_CP009494.1.
YP_890653.1. NC_008596.1.

3D structure databases

ProteinModelPortaliA0R665.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi246196.MSMEG_6440.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABK75948; ABK75948; MSMEG_6440.
AFP42698; AFP42698; MSMEI_6272.
GeneIDi4537741.
KEGGimsb:LJ00_31840.
msg:MSMEI_6272.
msm:MSMEG_6440.
PATRICi18085105. VBIMycSme59918_6265.

Phylogenomic databases

eggNOGiENOG4105CT3. Bacteria.
COG2072. LUCA.
HOGENOMiHOG000143616.
KOiK10215.
OMAiRRANIGG.
OrthoDBiPOG091H0APU.

Family and domain databases

Gene3Di3.50.50.60. 2 hits.
InterProiIPR023753. FAD/NAD-binding_dom.
IPR020946. Flavin_mOase-like.
[Graphical view]
PfamiPF00743. FMO-like. 1 hit.
[Graphical view]
SUPFAMiSSF51905. SSF51905. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiETHA_MYCS2
AccessioniPrimary (citable) accession number: A0R665
Secondary accession number(s): I7FUV7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 5, 2010
Last sequence update: January 9, 2007
Last modified: November 2, 2016
This is version 72 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.