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Protein

Putative 4-hydroxy-4-methyl-2-oxoglutarate aldolase

Gene

rraA

Organism
Mycobacterium smegmatis (strain ATCC 700084 / mc(2)155)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the aldol cleavage of 4-hydroxy-4-methyl-2-oxoglutarate (HMG) into 2 molecules of pyruvate. Also contains a secondary oxaloacetate (OAA) decarboxylase activity due to the common pyruvate enolate transition state formed following C-C bond cleavage in the retro-aldol and decarboxylation reactions (By similarity).By similarity

Catalytic activityi

4-hydroxy-4-methyl-2-oxoglutarate = 2 pyruvate.
Oxaloacetate = pyruvate + CO2.

Cofactori

a divalent metal cationBy similarityNote: Divalent metal cation.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei100SubstrateBy similarity1
Metal bindingi101Divalent metal cationBy similarity1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Lyase

Keywords - Ligandi

Metal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Putative 4-hydroxy-4-methyl-2-oxoglutarate aldolase (EC:4.1.3.17)
Short name:
HMG aldolase
Alternative name(s):
Oxaloacetate decarboxylase (EC:4.1.1.3)
Short name:
OAA decarboxylase
Regulator of ribonuclease activity homolog
RraA-like protein
Gene namesi
Name:rraA
Ordered Locus Names:MSMEG_6439, MSMEI_6271
OrganismiMycobacterium smegmatis (strain ATCC 700084 / mc(2)155)
Taxonomic identifieri246196 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaCorynebacterialesMycobacteriaceaeMycobacterium
Proteomesi
  • UP000006158 Componenti: Chromosome
  • UP000000757 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemoved1 Publication
ChainiPRO_10000138492 – 159Putative 4-hydroxy-4-methyl-2-oxoglutarate aldolaseAdd BLAST158

Interactioni

Subunit structurei

Homotrimer.By similarity

Protein-protein interaction databases

STRINGi246196.MSMEG_6439.

Structurei

3D structure databases

ProteinModelPortaliA0R664.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni78 – 81Substrate bindingBy similarity4

Sequence similaritiesi

Belongs to the class II aldolase/RraA-like family.Curated

Phylogenomic databases

eggNOGiENOG4108YYX. Bacteria.
COG0684. LUCA.
HOGENOMiHOG000252803.
KOiK02553.
OMAiEDNTLVR.
OrthoDBiPOG091H03SM.

Family and domain databases

Gene3Di3.50.30.40. 1 hit.
InterProiIPR010203. RraA.
IPR005493. RraA/RraA-like.
[Graphical view]
PfamiPF03737. Methyltransf_6. 1 hit.
[Graphical view]
SUPFAMiSSF89562. SSF89562. 1 hit.
TIGRFAMsiTIGR01935. NOT-MenG. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

A0R664-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSIEPRATAD LVDEIYPDVR SCDLQLQNYG GKIMFAGPVT TVRCFQDNAL
60 70 80 90 100
LKSILSTPGE GAVLVVDGAG SLHTALVGDV IAGLAADNGW SGVIVNGAVR
110 120 130 140 150
DAAALRTIDV GIKALGTNPR KGTKTGEGER DVEVSFGGVT FTPGDIAYCD

EDGIVVVTP
Length:159
Mass (Da):16,407
Last modified:January 9, 2007 - v1
Checksum:i108DAF5F15A38CD2
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000480 Genomic DNA. Translation: ABK71829.1.
CP001663 Genomic DNA. Translation: AFP42697.1.
RefSeqiWP_011731282.1. NZ_CP009494.1.
YP_890652.1. NC_008596.1.

Genome annotation databases

EnsemblBacteriaiABK71829; ABK71829; MSMEG_6439.
AFP42697; AFP42697; MSMEI_6271.
GeneIDi4535056.
KEGGimsb:LJ00_31835.
msg:MSMEI_6271.
msm:MSMEG_6439.
PATRICi18085103. VBIMycSme59918_6264.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000480 Genomic DNA. Translation: ABK71829.1.
CP001663 Genomic DNA. Translation: AFP42697.1.
RefSeqiWP_011731282.1. NZ_CP009494.1.
YP_890652.1. NC_008596.1.

3D structure databases

ProteinModelPortaliA0R664.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi246196.MSMEG_6439.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABK71829; ABK71829; MSMEG_6439.
AFP42697; AFP42697; MSMEI_6271.
GeneIDi4535056.
KEGGimsb:LJ00_31835.
msg:MSMEI_6271.
msm:MSMEG_6439.
PATRICi18085103. VBIMycSme59918_6264.

Phylogenomic databases

eggNOGiENOG4108YYX. Bacteria.
COG0684. LUCA.
HOGENOMiHOG000252803.
KOiK02553.
OMAiEDNTLVR.
OrthoDBiPOG091H03SM.

Family and domain databases

Gene3Di3.50.30.40. 1 hit.
InterProiIPR010203. RraA.
IPR005493. RraA/RraA-like.
[Graphical view]
PfamiPF03737. Methyltransf_6. 1 hit.
[Graphical view]
SUPFAMiSSF89562. SSF89562. 1 hit.
TIGRFAMsiTIGR01935. NOT-MenG. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiRRAAH_MYCS2
AccessioniPrimary (citable) accession number: A0R664
Secondary accession number(s): I7GAU1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: January 9, 2007
Last modified: November 2, 2016
This is version 66 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.