A0R647 (BFRB_MYCS2) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Ferritin BfrB
EC=1.16.3.1

Alternative name(s):
Non-heme ferritin Ftn

Gene names

Name:	bfrB
Synonyms:	ftn
Ordered Locus Names:	MSMEG_6422, MSMEI_6254

Organism

Mycobacterium smegmatis (strain ATCC 700084 / mc(2)155) [Reference proteome] [HAMAP]

Taxonomic identifier

246196 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Actinobacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Corynebacterineae › Mycobacteriaceae › Mycobacterium › Mycobacterium smegmatis

Protein attributes

Sequence length	181 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Iron-storage protein that displays ferroxidase activity, catalyzing the oxidation of Fe2+ ions into Fe3+ ions, that can then be deposited as a ferric-oxide mineral core within the central cavity of the protein complex By similarity.
Catalytic activity	4 Fe2+ + 4 H+ + O2 = 4 Fe3+ + 2 H2O.
Subunit structure	Homooligomer of 24 subunits that are packed together to form an approximately spherical molecule with a central cavity, in which large amounts of iron can be stored By similarity.
Sequence similarities	Belongs to the ferritin family. Prokaryotic subfamily. Contains 1 ferritin-like diiron domain.

Ontologies

Keywords
Biological process	Iron storage
Ligand	Iron Metal-binding
Molecular function	Oxidoreductase
PTM	Isopeptide bond Ubl conjugation
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	cellular iron ion homeostasis Inferred from electronic annotation. Source: UniProtKB-KW iron ion transport Inferred from electronic annotation. Source: InterPro
Molecular_function	ferric iron binding Inferred from electronic annotation. Source: InterPro ferroxidase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 181	181	Ferritin BfrB		PRO_0000396101
Regions
	Domain	5 – 150	146	Ferritin-like diiron
Sites
	Metal binding	22	1	Iron 1 By similarity
	Metal binding	55	1	Iron 1 By similarity
	Metal binding	55	1	Iron 2 By similarity
	Metal binding	58	1	Iron 1 By similarity
	Metal binding	99	1	Iron 2 By similarity
	Metal binding	132	1	Iron 2 By similarity
Amino acid modifications
	Cross-link	10		Isoglutamyl lysine isopeptide (Lys-Gln) (interchain with Q-Cter in protein Pup)

Sequences

	Sequence		Length	Mass (Da)
	A0R647 [UniParc]. Last modified January 9, 2007. Version 1. Checksum: 521F00899CA34B8B	FASTA	181	20,040
	10 20 30 40 50 60 MTNSGALDTK FHALIQDQIR SEFTASQQYI AIAVFFDGAD LPQLAKHFYA QALEERNHAM 70 80 90 100 110 120 MLVQYLLDRD VEVEIPGIDP VCNNFTTPRD ALALALDQER TVTEQISRLA SVARDEGDHL 130 140 150 160 170 180 GEQFMQWFLK EQVEEVAAMT TLVRIADRAG SNLFHIEDFV AREMSAAGAD PTAPRAAGGA L

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	Fleischmann R.D., Dodson R.J., Haft D.H., Merkel J.S., Nelson W.C., Fraser C.M. Submitted (OCT-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 700084 / mc(2)155.
[2]	"Interrupted coding sequences in Mycobacterium smegmatis: authentic mutations or sequencing errors?" Deshayes C., Perrodou E., Gallien S., Euphrasie D., Schaeffer C., Van-Dorsselaer A., Poch O., Lecompte O., Reyrat J.-M. Genome Biol. 8:R20.1-R20.9(2007) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 700084 / mc(2)155.
[3]	"Ortho-proteogenomics: multiple proteomes investigation through orthology and a new MS-based protocol." Gallien S., Perrodou E., Carapito C., Deshayes C., Reyrat J.-M., Van Dorsselaer A., Poch O., Schaeffer C., Lecompte O. Genome Res. 19:128-135(2009) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 700084 / mc(2)155.
[4]	"Expansion of the mycobacterial 'PUPylome'." Watrous J., Burns K., Liu W.T., Patel A., Hook V., Bafna V., Barry C.E. III, Bark S., Dorrestein P.C. Mol. Biosyst. 6:376-385(2010) [PubMed] [Europe PMC] [Abstract] Cited for: PUPYLATION AT LYS-10, IDENTIFICATION BY MASS SPECTROMETRY.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000480 Genomic DNA. Translation: ABK71142.1. CP001663 Genomic DNA. Translation: AFP42680.1.
RefSeq	YP_006570975.1. NC_018289.1. YP_890635.1. NC_008596.1.
3D structure databases
ProteinModelPortal	A0R647.
ModBase	Search...
Protein-protein interaction databases
STRING	246196.MSMEG_6422.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	ABK71142; ABK71142; MSMEG_6422.
GeneID	13427584. 4532622.
KEGG	msg:MSMEI_6254. msm:MSMEG_6422.
PATRIC	18085073. VBIMycSme59918_6249.
Phylogenomic databases
eggNOG	COG1528.
HOGENOM	HOG000223382.
OMA	QYFLDRD.
ProtClustDB	CLSK2778415.
Enzyme and pathway databases
BioCyc	MSME246196:GJ4Y-6421-MONOMER.
Family and domain databases
Gene3D	1.20.1260.10. 1 hit.
InterPro	IPR001519. Ferritin. IPR009040. Ferritin-like_diiron. IPR009078. Ferritin-like_SF. IPR012347. Ferritin-rel. IPR008331. Ferritin_DPS_dom. [Graphical view]
PANTHER	PTHR11431. PTHR11431. 1 hit.
Pfam	PF00210. Ferritin. 1 hit. [Graphical view]
SUPFAM	SSF47240. Ferritin/RR_like. 1 hit.
PROSITE	PS50905. FERRITIN_LIKE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

BFRB_MYCS2

Accession

Primary (citable) accession number: A0R647
Secondary accession number(s): I7FMX7

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 10, 2010
Last sequence update:	January 9, 2007
Last modified:	May 1, 2013
This is version 49 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program