Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Galactofuranosyl transferase GlfT2

Gene

glfT2

Organism
Mycobacterium smegmatis (strain ATCC 700084 / mc(2)155)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Protein inferred from homologyi

Functioni

Involved in the polymerization of the arabinogalactan (AG) region of the mycolylarabinogalactan-peptidoglycan (mAGP) complex, an essential component the mycobacteria cell wall, through successively beta-D-(1->5) and beta-D-(1->6)-galactofuranosyltransferases activities. It transfers the galactofuranosyl (Galf) unit from UDP-galactofuranosyl (UDP-Galf) onto the galactofuranosyl-rhamnosyl-GlcNAc-pyrophosphoryl-undecaprenyl (Galf-Rha-GlcNAc-PP-C55), yielding polygalactofuranosyl-rhamnosyl-GlcNAc-pyrophosphoryl-undecaprenyl ((Galf)x-Rha-GlcNAc-PP-C55) (By similarity).By similarity

Catalytic activityi

28 UDP-alpha-D-galactofuranose + beta-D-galactofuranosyl-(1->5)-beta-D-galactofuranosyl-(1->4)-alpha-L-rhamnopyranosyl-(1->3)-N-acetyl-alpha-D-glucosaminyl-diphospho-trans,octacis-decaprenol = 28 UDP + (beta-D-galactofuranosyl-(1->5)-beta-D-galactofuranosyl-(1->6))(14)-beta-D-galactofuranosyl-(1->5)-beta-D-galactofuranosyl-(1->4)-alpha-L-rhamnopyranosyl-(1->3)-N-acetyl-alpha-D-glucosaminyl-diphospho-trans,octacis-decaprenol.

Cofactori

Mn2+By similarity, Mg2+By similarity

Pathwayi: cell wall polysaccharide biosynthesis

This protein is involved in the pathway cell wall polysaccharide biosynthesis, which is part of Cell wall biogenesis.
View all proteins of this organism that are known to be involved in the pathway cell wall polysaccharide biosynthesis and in Cell wall biogenesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei182SubstrateBy similarity1
Binding sitei211SubstrateBy similarity1
Binding sitei240SubstrateBy similarity1
Metal bindingi267ManganeseBy similarity1
Binding sitei267SubstrateBy similarity1
Metal bindingi269ManganeseBy similarity1
Active sitei283Proton acceptorBy similarity1
Metal bindingi408ManganeseBy similarity1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Transferase

Keywords - Biological processi

Cell wall biogenesis/degradation

Keywords - Ligandi

Magnesium, Manganese, Metal-binding

Enzyme and pathway databases

UniPathwayiUPA00963.

Protein family/group databases

CAZyiGT2. Glycosyltransferase Family 2.

Names & Taxonomyi

Protein namesi
Recommended name:
Galactofuranosyl transferase GlfT2 (EC:2.4.1.288)
Short name:
GalTr
Alternative name(s):
Beta-D-(1->5)galactofuranosyltransferase
Beta-D-(1->6)galactofuranosyltransferase
Galactofuranosylgalactofuranosylrhamnosyl-N-acetylglucosaminyl-diphospho-decaprenol beta-1,5/1,6-galactofuranosyltransferase
UDP-Galf:alpha-3-L-rhamnosyl-alpha-D-GlcNAc-pyrophosphate polyprenol, UDP-galactofuranosyl transferase
Gene namesi
Name:glfT2
Ordered Locus Names:MSMEG_6403, MSMEI_6235
OrganismiMycobacterium smegmatis (strain ATCC 700084 / mc(2)155)
Taxonomic identifieri246196 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaCorynebacterialesMycobacteriaceaeMycobacterium
Proteomesi
  • UP000006158 Componenti: Chromosome
  • UP000000757 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003953581 – 646Galactofuranosyl transferase GlfT2Add BLAST646

Interactioni

Subunit structurei

Dimer of dimers.By similarity

Protein-protein interaction databases

STRINGi246196.MSMEG_6403.

Structurei

3D structure databases

ProteinModelPortaliA0R628.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the glycosyltransferase 2 family.Curated

Phylogenomic databases

eggNOGiENOG410602S. Bacteria.
COG1216. LUCA.
HOGENOMiHOG000247585.
KOiK16650.
OMAiDVDFNGW.
OrthoDBiPOG091H0B36.

Family and domain databases

Gene3Di3.90.550.10. 1 hit.
InterProiIPR029044. Nucleotide-diphossugar_trans.
[Graphical view]
SUPFAMiSSF53448. SSF53448. 2 hits.

Sequencei

Sequence statusi: Complete.

A0R628-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSDIPSGALE ADESRAVSLL SRVILPRPGE PLDVRKLYIE ESTTNSRRAH
60 70 80 90 100
APTRTSLEIG PESEVSFATY FNAFPASYWR RWSTLDTVVL RVELTGTARV
110 120 130 140 150
DVYRSKATGA RITVGGAPVA GEGDGPAAVE FEIDLTPFED GGWIWFDITT
160 170 180 190 200
NTAVRVHSAG WYAPVPAPGR ANVAVGIPTF NRPADCVNAL AALTSDPLVD
210 220 230 240 250
EVISAVIVSD QGTSKAKDHP GFADAAARLG DRLSIHNQPN LGGSGGYSRV
260 270 280 290 300
MYEALKNTDC EQILFMDDDI RIEPDSILRA LAMNRFAKSP ILVGGQMLNL
310 320 330 340 350
QEPSHLHVMG EVVDRANFMW TAAPNAEYDH DFAKFKLSDA EEPRTKLLHR
360 370 380 390 400
RVDVDFNGWW MCMIPRQIAE ELGQPLPLFI KWDDAEYGLR AGEHGYGTVT
410 420 430 440 450
LPGAAIWHMA WSDKDDAIDW QAYFHLRNRL VVAALHWDGP VSGLIASHLK
460 470 480 490 500
ATIKHLLCLE YSTVAIQNKA MEDFLAGPEN LFSILESAMP DVRKLRSQYP
510 520 530 540 550
DAVVLPGATS LPPASDMRRK KIAIPVSKPA IAVNLARGVV HQLRSHDPET
560 570 580 590 600
HVRPQINVAT QDARWFSLCR VDGVTVTTAD GRGVVYRQRD RAKMFALLRA
610 620 630 640
SLRQQARLAR KFDRMRKVYR DALPMLTSTQ KWESVLLTET PEKVGR
Length:646
Mass (Da):71,573
Last modified:January 9, 2007 - v1
Checksum:i58E7858070950B59
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000480 Genomic DNA. Translation: ABK72427.1.
CP001663 Genomic DNA. Translation: AFP42661.1.
RefSeqiWP_011731265.1. NZ_CP009494.1.
YP_890616.1. NC_008596.1.

Genome annotation databases

EnsemblBacteriaiABK72427; ABK72427; MSMEG_6403.
AFP42661; AFP42661; MSMEI_6235.
GeneIDi4536763.
KEGGimsb:LJ00_31650.
msg:MSMEI_6235.
msm:MSMEG_6403.
PATRICi18085033. VBIMycSme59918_6229.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000480 Genomic DNA. Translation: ABK72427.1.
CP001663 Genomic DNA. Translation: AFP42661.1.
RefSeqiWP_011731265.1. NZ_CP009494.1.
YP_890616.1. NC_008596.1.

3D structure databases

ProteinModelPortaliA0R628.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi246196.MSMEG_6403.

Protein family/group databases

CAZyiGT2. Glycosyltransferase Family 2.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABK72427; ABK72427; MSMEG_6403.
AFP42661; AFP42661; MSMEI_6235.
GeneIDi4536763.
KEGGimsb:LJ00_31650.
msg:MSMEI_6235.
msm:MSMEG_6403.
PATRICi18085033. VBIMycSme59918_6229.

Phylogenomic databases

eggNOGiENOG410602S. Bacteria.
COG1216. LUCA.
HOGENOMiHOG000247585.
KOiK16650.
OMAiDVDFNGW.
OrthoDBiPOG091H0B36.

Enzyme and pathway databases

UniPathwayiUPA00963.

Family and domain databases

Gene3Di3.90.550.10. 1 hit.
InterProiIPR029044. Nucleotide-diphossugar_trans.
[Graphical view]
SUPFAMiSSF53448. SSF53448. 2 hits.
ProtoNetiSearch...

Entry informationi

Entry nameiGLFT2_MYCS2
AccessioniPrimary (citable) accession number: A0R628
Secondary accession number(s): I7GFR0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 13, 2010
Last sequence update: January 9, 2007
Last modified: November 2, 2016
This is version 62 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Miscellaneous

Was identified as a high-confidence drug target.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.