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Protein

Galactofuranosyltransferase GlfT2

Gene

glfT2

Organism
Mycobacterium smegmatis (strain ATCC 700084 / mc(2)155)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Involved in the galactan polymerization of the arabinogalactan (AG) region of the mycolylarabinogalactan-peptidoglycan (mAGP) complex, an essential component of the mycobacteria cell wall. Thus, successively transfers approximately 28 galactofuranosyl (Galf) residues from UDP-galactofuranose (UDP-Galf) onto the galactofuranosyl-galactofuranosyl-rhamnosyl-GlcNAc-diphospho-decaprenol (Galf-Galf-Rha-GlcNAc-PP-C50) acceptor produced by GlfT1, with alternating 1->5 and 1->6 links, forming a galactan domain with approximately 30 galactofuranosyl residues.By similarity

Catalytic activityi

28 UDP-alpha-D-galactofuranose + beta-D-galactofuranosyl-(1->5)-beta-D-galactofuranosyl-(1->4)-alpha-L-rhamnopyranosyl-(1->3)-N-acetyl-alpha-D-glucosaminyl-diphospho-trans,octacis-decaprenol = 28 UDP + (beta-D-galactofuranosyl-(1->5)-beta-D-galactofuranosyl-(1->6))(14)-beta-D-galactofuranosyl-(1->5)-beta-D-galactofuranosyl-(1->4)-alpha-L-rhamnopyranosyl-(1->3)-N-acetyl-alpha-D-glucosaminyl-diphospho-trans,octacis-decaprenol.By similarity

Cofactori

Mn2+By similarity, Mg2+By similarity

Pathwayi: cell wall polysaccharide biosynthesis

This protein is involved in the pathway cell wall polysaccharide biosynthesis, which is part of Cell wall biogenesis.By similarity
View all proteins of this organism that are known to be involved in the pathway cell wall polysaccharide biosynthesis and in Cell wall biogenesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei182UDP-GalfBy similarity1
Binding sitei211UDP-GalfBy similarity1
Binding sitei240UDP-GalfBy similarity1
Metal bindingi267ManganeseBy similarity1
Binding sitei267UDP-GalfBy similarity1
Metal bindingi269ManganeseBy similarity1
Active sitei384Proton acceptorBy similarity1
Metal bindingi408ManganeseBy similarity1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywordsi

Molecular functionGlycosyltransferase, Transferase
Biological processCell wall biogenesis/degradation
LigandMagnesium, Manganese, Metal-binding

Enzyme and pathway databases

UniPathwayiUPA00963.

Protein family/group databases

CAZyiGT2. Glycosyltransferase Family 2.

Names & Taxonomyi

Protein namesi
Recommended name:
Galactofuranosyltransferase GlfT2By similarity (EC:2.4.1.288By similarity)
Short name:
GalTr 2By similarity
Alternative name(s):
Arabinogalactan galactosyltransferase 2By similarity
Galactofuranosylgalactofuranosylrhamnosyl-N-acetylglucosaminyl-diphospho-decaprenol beta-1,5/1,6-galactofuranosyltransferaseBy similarity
Polymerizing galactofuranosyltransferase GlfT2By similarity
Gene namesi
Name:glfT2By similarity
Ordered Locus Names:MSMEG_6403, MSMEI_6235
OrganismiMycobacterium smegmatis (strain ATCC 700084 / mc(2)155)
Taxonomic identifieri246196 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaCorynebacterialesMycobacteriaceaeMycobacterium
Proteomesi
  • UP000006158 Componenti: Chromosome
  • UP000000757 Componenti: Chromosome

Subcellular locationi

  • Cell membrane By similarity

GO - Cellular componenti

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003953581 – 646Galactofuranosyltransferase GlfT2Add BLAST646

Interactioni

Subunit structurei

Homotetramer.By similarity

Protein-protein interaction databases

STRINGi246196.MSMEG_6403.

Structurei

3D structure databases

ProteinModelPortaliA0R628.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the glycosyltransferase 2 family.Curated

Phylogenomic databases

eggNOGiENOG410602S. Bacteria.
COG1216. LUCA.
HOGENOMiHOG000247585.
KOiK16650.
OMAiDVDFNGW.
OrthoDBiPOG091H0B36.

Family and domain databases

Gene3Di3.90.550.10. 1 hit.
InterProiIPR029044. Nucleotide-diphossugar_trans.
[Graphical view]
SUPFAMiSSF53448. SSF53448. 2 hits.

Sequencei

Sequence statusi: Complete.

A0R628-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSDIPSGALE ADESRAVSLL SRVILPRPGE PLDVRKLYIE ESTTNSRRAH
60 70 80 90 100
APTRTSLEIG PESEVSFATY FNAFPASYWR RWSTLDTVVL RVELTGTARV
110 120 130 140 150
DVYRSKATGA RITVGGAPVA GEGDGPAAVE FEIDLTPFED GGWIWFDITT
160 170 180 190 200
NTAVRVHSAG WYAPVPAPGR ANVAVGIPTF NRPADCVNAL AALTSDPLVD
210 220 230 240 250
EVISAVIVSD QGTSKAKDHP GFADAAARLG DRLSIHNQPN LGGSGGYSRV
260 270 280 290 300
MYEALKNTDC EQILFMDDDI RIEPDSILRA LAMNRFAKSP ILVGGQMLNL
310 320 330 340 350
QEPSHLHVMG EVVDRANFMW TAAPNAEYDH DFAKFKLSDA EEPRTKLLHR
360 370 380 390 400
RVDVDFNGWW MCMIPRQIAE ELGQPLPLFI KWDDAEYGLR AGEHGYGTVT
410 420 430 440 450
LPGAAIWHMA WSDKDDAIDW QAYFHLRNRL VVAALHWDGP VSGLIASHLK
460 470 480 490 500
ATIKHLLCLE YSTVAIQNKA MEDFLAGPEN LFSILESAMP DVRKLRSQYP
510 520 530 540 550
DAVVLPGATS LPPASDMRRK KIAIPVSKPA IAVNLARGVV HQLRSHDPET
560 570 580 590 600
HVRPQINVAT QDARWFSLCR VDGVTVTTAD GRGVVYRQRD RAKMFALLRA
610 620 630 640
SLRQQARLAR KFDRMRKVYR DALPMLTSTQ KWESVLLTET PEKVGR
Length:646
Mass (Da):71,573
Last modified:January 9, 2007 - v1
Checksum:i58E7858070950B59
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000480 Genomic DNA. Translation: ABK72427.1.
CP001663 Genomic DNA. Translation: AFP42661.1.
RefSeqiWP_011731265.1. NZ_CP009494.1.
YP_890616.1. NC_008596.1.

Genome annotation databases

EnsemblBacteriaiABK72427; ABK72427; MSMEG_6403.
AFP42661; AFP42661; MSMEI_6235.
GeneIDi4536763.
KEGGimsb:LJ00_31650.
msg:MSMEI_6235.
msm:MSMEG_6403.
PATRICi18085033. VBIMycSme59918_6229.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000480 Genomic DNA. Translation: ABK72427.1.
CP001663 Genomic DNA. Translation: AFP42661.1.
RefSeqiWP_011731265.1. NZ_CP009494.1.
YP_890616.1. NC_008596.1.

3D structure databases

ProteinModelPortaliA0R628.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi246196.MSMEG_6403.

Protein family/group databases

CAZyiGT2. Glycosyltransferase Family 2.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABK72427; ABK72427; MSMEG_6403.
AFP42661; AFP42661; MSMEI_6235.
GeneIDi4536763.
KEGGimsb:LJ00_31650.
msg:MSMEI_6235.
msm:MSMEG_6403.
PATRICi18085033. VBIMycSme59918_6229.

Phylogenomic databases

eggNOGiENOG410602S. Bacteria.
COG1216. LUCA.
HOGENOMiHOG000247585.
KOiK16650.
OMAiDVDFNGW.
OrthoDBiPOG091H0B36.

Enzyme and pathway databases

UniPathwayiUPA00963.

Family and domain databases

Gene3Di3.90.550.10. 1 hit.
InterProiIPR029044. Nucleotide-diphossugar_trans.
[Graphical view]
SUPFAMiSSF53448. SSF53448. 2 hits.
ProtoNetiSearch...

Entry informationi

Entry nameiGLFT2_MYCS2
AccessioniPrimary (citable) accession number: A0R628
Secondary accession number(s): I7GFR0
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 13, 2010
Last sequence update: January 9, 2007
Last modified: January 18, 2017
This is version 63 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.