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Protein

Catalase-peroxidase 1

Gene

katG1

Organism
Mycobacterium smegmatis (strain ATCC 700084 / mc(2)155)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Protein inferred from homologyi

Functioni

Bifunctional enzyme with both catalase and broad-spectrum peroxidase activity. May play a role in the intracellular survival of mycobacteria.

Catalytic activityi

Donor + H2O2 = oxidized donor + 2 H2O.UniRule annotation
2 H2O2 = O2 + 2 H2O.UniRule annotation

Cofactori

heme bNote: Binds 1 heme b (iron(II)-protoporphyrin IX) group per dimer.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei110Transition state stabilizerUniRule annotation1
Active sitei114Proton acceptorUniRule annotation1
Metal bindingi277Iron (heme axial ligand)UniRule annotation1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase, Peroxidase

Keywords - Biological processi

Hydrogen peroxide

Keywords - Ligandi

Heme, Iron, Metal-binding

Protein family/group databases

PeroxiBasei3550. MsmCP01_mc2155.

Names & Taxonomyi

Protein namesi
Recommended name:
Catalase-peroxidase 1UniRule annotation (EC:1.11.1.21UniRule annotation)
Short name:
CP 1UniRule annotation
Alternative name(s):
Peroxidase/catalase 1UniRule annotation
Gene namesi
Name:katG1UniRule annotation
Ordered Locus Names:MSMEG_6384, MSMEI_6216
OrganismiMycobacterium smegmatis (strain ATCC 700084 / mc(2)155)
Taxonomic identifieri246196 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaCorynebacterialesMycobacteriaceaeMycobacterium
Proteomesi
  • UP000006158 Componenti: Chromosome
  • UP000000757 Componenti: Chromosome

Pathology & Biotechi

Disruption phenotypei

Defects cause isoniazid (INH) resistance.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002935961 – 739Catalase-peroxidase 1Add BLAST739

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Cross-linki113 ↔ 236Tryptophyl-tyrosyl-methioninium (Trp-Tyr) (with M-262)UniRule annotation
Cross-linki236 ↔ 262Tryptophyl-tyrosyl-methioninium (Tyr-Met) (with W-113)UniRule annotation

Post-translational modificationi

The covalent Trp-Tyr-Met adduct is important for the catalase, but not the peroxidase activity of the enzyme.UniRule annotation

Interactioni

Subunit structurei

Homodimer or homotetramer.UniRule annotation

Protein-protein interaction databases

STRINGi246196.MSMEG_6384.

Structurei

3D structure databases

ProteinModelPortaliA0R609.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the peroxidase family. Peroxidase/catalase subfamily.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105C1X. Bacteria.
COG0376. LUCA.
HOGENOMiHOG000218110.
KOiK03782.
OMAiNEMLAND.
OrthoDBiPOG091H05R1.

Family and domain databases

HAMAPiMF_01961. Catal_peroxid. 1 hit.
InterProiIPR000763. Catalase_peroxidase.
IPR010255. Haem_peroxidase.
IPR002016. Haem_peroxidase_pln/fun/bac.
IPR019794. Peroxidases_AS.
IPR019793. Peroxidases_heam-ligand_BS.
[Graphical view]
PfamiPF00141. peroxidase. 2 hits.
[Graphical view]
PRINTSiPR00460. BPEROXIDASE.
PR00458. PEROXIDASE.
SUPFAMiSSF48113. SSF48113. 2 hits.
TIGRFAMsiTIGR00198. cat_per_HPI. 1 hit.
PROSITEiPS00435. PEROXIDASE_1. 1 hit.
PS00436. PEROXIDASE_2. 1 hit.
PS50873. PEROXIDASE_4. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

A0R609-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPEDRPIEDS PPIGEAQTDA PAGGCPAGFG RIKPPVAGGS NRDWWPNQLN
60 70 80 90 100
LKILQKNPDV INPLDEDFDY RSAVQNLDVD ALRADIVEVM HTSQDWWPAD
110 120 130 140 150
FGHYGPLFIR MAWHAAGTYR VSDGRGGAGA GMQRFAPLNS WPDNASLDKA
160 170 180 190 200
RRLLWPVKKK YGKNLSWADL IVYAGNVALE DMGFRTAGFA FGREDRWEPE
210 220 230 240 250
EDVYWGPEQE WLDDKRYTGE RDLENPLAAV QMGLIYVNPE GPNGNPDPQA
260 270 280 290 300
SAIDIRETFG RMAMNDVETA ALIVGGHTFG KTHGNGDASL VGPEPEAAPL
310 320 330 340 350
EEVGLGWRNP QGTGVGKDAI TSGLEVTWTH TPTKWDNSFL EILYGNEWEL
360 370 380 390 400
TKSPAGANQW KPKDNGWANS VPLAHEDGKT HPSMLTSDLA LRVDPIYEQI
410 420 430 440 450
TRRWLDHPEE LAEEFAKAWF KLLHRDMGPV TRYLGPEVPK DTWLWQDNIP
460 470 480 490 500
AGNDLSDDEV AKLKELIADS GLTVSQLVST AWKAASTFRS SDLRGGANGG
510 520 530 540 550
RIRLQPQLGW EANEPDELAQ VVRKYEEIQK ASGINVSFAD LVVLGGNVGV
560 570 580 590 600
EKAAKAAGFD VTVPFTPGRG DATQEETDVD SFAYLEPKAD GFRNYLGKGS
610 620 630 640 650
DLPAEFKLID RANLLGLSAP EMTTLVGGLR VLDVNHGGTK HGVLTDKPGA
660 670 680 690 700
LTTDFFVNLL DMSTAWKPSP ADDGTYIGTD RATGSPKWTG TRVDLVFASN
710 720 730
SQLRALAEVY AEDDSKEKFV KDFVAAWTKV MDADRFDVA
Length:739
Mass (Da):81,115
Last modified:January 9, 2007 - v1
Checksum:i5E87F6B884D832ED
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti374 – 378AHEDG → PTRTA in CAA67268 (PubMed:8870251).Curated5

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X98718 Genomic DNA. Translation: CAA67268.1.
CP000480 Genomic DNA. Translation: ABK73944.1.
CP001663 Genomic DNA. Translation: AFP42642.1.
RefSeqiWP_011731250.1. NZ_CP009494.1.
YP_890597.1. NC_008596.1.

Genome annotation databases

EnsemblBacteriaiABK73944; ABK73944; MSMEG_6384.
AFP42642; AFP42642; MSMEI_6216.
GeneIDi4536370.
KEGGimsb:LJ00_31555.
msg:MSMEI_6216.
msm:MSMEG_6384.
PATRICi18084997. VBIMycSme59918_6211.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X98718 Genomic DNA. Translation: CAA67268.1.
CP000480 Genomic DNA. Translation: ABK73944.1.
CP001663 Genomic DNA. Translation: AFP42642.1.
RefSeqiWP_011731250.1. NZ_CP009494.1.
YP_890597.1. NC_008596.1.

3D structure databases

ProteinModelPortaliA0R609.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi246196.MSMEG_6384.

Protein family/group databases

PeroxiBasei3550. MsmCP01_mc2155.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABK73944; ABK73944; MSMEG_6384.
AFP42642; AFP42642; MSMEI_6216.
GeneIDi4536370.
KEGGimsb:LJ00_31555.
msg:MSMEI_6216.
msm:MSMEG_6384.
PATRICi18084997. VBIMycSme59918_6211.

Phylogenomic databases

eggNOGiENOG4105C1X. Bacteria.
COG0376. LUCA.
HOGENOMiHOG000218110.
KOiK03782.
OMAiNEMLAND.
OrthoDBiPOG091H05R1.

Family and domain databases

HAMAPiMF_01961. Catal_peroxid. 1 hit.
InterProiIPR000763. Catalase_peroxidase.
IPR010255. Haem_peroxidase.
IPR002016. Haem_peroxidase_pln/fun/bac.
IPR019794. Peroxidases_AS.
IPR019793. Peroxidases_heam-ligand_BS.
[Graphical view]
PfamiPF00141. peroxidase. 2 hits.
[Graphical view]
PRINTSiPR00460. BPEROXIDASE.
PR00458. PEROXIDASE.
SUPFAMiSSF48113. SSF48113. 2 hits.
TIGRFAMsiTIGR00198. cat_per_HPI. 1 hit.
PROSITEiPS00435. PEROXIDASE_1. 1 hit.
PS00436. PEROXIDASE_2. 1 hit.
PS50873. PEROXIDASE_4. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiKATG1_MYCS2
AccessioniPrimary (citable) accession number: A0R609
Secondary accession number(s): I7GAP8, O05763, Q59557
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 10, 2007
Last sequence update: January 9, 2007
Last modified: November 2, 2016
This is version 68 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.