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Protein

Galactofuranosyltransferase GlfT1

Gene

glfT1

Organism
Mycobacterium smegmatis (strain ATCC 700084 / mc(2)155)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Involved in the biosynthesis of the arabinogalactan (AG) region of the mycolylarabinogalactan-peptidoglycan (mAGP) complex, an essential component of the mycobacterial cell wall. Catalyzes the transfer of the first two galactofuranosyl (Galf) units from UDP-galactofuranose (UDP-Galf) onto the rhamnosyl-GlcNAc-diphospho-decaprenol (Rha-GlcNAc-PP-C50) acceptor, yielding galactofuranosyl-galactofuranosyl-rhamnosyl-GlcNAc-diphospho-decaprenol (Galf-Galf-Rha-GlcNAc-PP-C50). Thus, GlfT1 is the initiator of galactan synthesis, while GlfT2 continues with the subsequent polymerization events.1 Publication

Catalytic activityi

2 UDP-alpha-D-galactofuranose + alpha-L-rhamnopyranosyl-(1->3)-N-acetyl-alpha-D-glucosaminyl-diphospho-trans,octacis-decaprenol = 2 UDP + beta-D-galactofuranosyl-(1->5)-beta-D-galactofuranosyl-(1->4)-alpha-L-rhamnopyranosyl-(1->3)-N-acetyl-alpha-D-glucosaminyl-diphospho-trans,octacis-decaprenol.1 Publication

Pathwayi: cell wall polysaccharide biosynthesis

This protein is involved in the pathway cell wall polysaccharide biosynthesis, which is part of Cell wall biogenesis.1 Publication
View all proteins of this organism that are known to be involved in the pathway cell wall polysaccharide biosynthesis and in Cell wall biogenesis.

GO - Molecular functioni

  • transferase activity Source: UniProtKB
  • transferase activity, transferring glycosyl groups Source: UniProtKB

GO - Biological processi

  • capsule polysaccharide biosynthetic process Source: UniProtKB-UniPathway
  • cell wall macromolecule biosynthetic process Source: UniProtKB
  • cell wall organization Source: UniProtKB

Keywordsi

Molecular functionGlycosyltransferase, Transferase
Biological processCell wall biogenesis/degradation

Enzyme and pathway databases

UniPathwayiUPA00963.

Protein family/group databases

CAZyiGT2. Glycosyltransferase Family 2.

Names & Taxonomyi

Protein namesi
Recommended name:
Galactofuranosyltransferase GlfT1Curated (EC:2.4.1.2871 Publication)
Short name:
GalTr 1Curated
Alternative name(s):
Arabinogalactan galactosyltransferase 1Curated
Rhamnopyranosyl-N-acetylglucosaminyl-diphospho-decaprenol beta-1,4/1,5-galactofuranosyltransferase
UDP-Galf:alpha-3-L-rhamnosyl-alpha-D-GlcNAc-pyrophosphate polyprenol, galactofuranosyl transferase
Gene namesi
Name:glfT11 Publication
Ordered Locus Names:MSMEG_6367, MSMEI_6199
OrganismiMycobacterium smegmatis (strain ATCC 700084 / mc(2)155)
Taxonomic identifieri246196 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaCorynebacterialesMycobacteriaceaeMycobacterium
Proteomesi
  • UP000006158 Componenti: Chromosome
  • UP000000757 Componenti: Chromosome

Subcellular locationi

  • Cell membrane By similarity
  • Secretedcell wall By similarity

GO - Cellular componenti

Keywords - Cellular componenti

Cell membrane, Cell wall, Membrane, Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003953571 – 302Galactofuranosyltransferase GlfT1Add BLAST302

Interactioni

Protein-protein interaction databases

STRINGi246196.MSMEG_6367.

Structurei

3D structure databases

ProteinModelPortaliA0R5Z2.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the glycosyltransferase 2 family.Curated

Phylogenomic databases

eggNOGiENOG4108MKI. Bacteria.
COG1216. LUCA.
HOGENOMiHOG000247165.
KOiK16649.
OMAiTCLQTAY.
OrthoDBiPOG091H09G0.

Family and domain databases

Gene3Di3.90.550.10. 1 hit.
InterProiView protein in InterPro
IPR029044. Nucleotide-diphossugar_trans.
SUPFAMiSSF53448. SSF53448. 1 hit.

Sequencei

Sequence statusi: Complete.

A0R5Z2-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTHTEVVCAV VVTHRRRELL ATSLDAVVSQ DRKPDHLIVV DNDNDPQVRE
60 70 80 90 100
LVTGQPVPST YLGSRRNLGG AGGFALGMLH ALALGADWIW LADDDGRPAD
110 120 130 140 150
TTVLSTLLSC AHTHSLAEVS PMVCNLDDPQ RLAFPLRRGL VWRRLTSELR
160 170 180 190 200
TDSSSSSGDL LPGIASLFNG ALFRADTVDA VGVPDLRLFV RGDEVELHRR
210 220 230 240 250
LVRSGLPFGT CLTASYLHPC GTDEFKPILG GRMHTQYPDD ETKRFFTYRN
260 270 280 290 300
RGYLLSQPGL RKLLPQEWLR FGWYFLVSRR DLAGLREWIR LRRLGRRERF

QR
Length:302
Mass (Da):34,041
Last modified:January 9, 2007 - v1
Checksum:i407E1D4CA50DA4BD
GO

Sequence cautioni

The sequence AFP42625 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000480 Genomic DNA. Translation: ABK69689.1.
CP001663 Genomic DNA. Translation: AFP42625.1. Different initiation.
RefSeqiWP_011731236.1. NZ_CP009494.1.
YP_890580.1. NC_008596.1.

Genome annotation databases

EnsemblBacteriaiABK69689; ABK69689; MSMEG_6367.
AFP42625; AFP42625; MSMEI_6199.
GeneIDi4537393.
KEGGimsb:LJ00_31470.
msg:MSMEI_6199.
msm:MSMEG_6367.
PATRICifig|246196.19.peg.6195.

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.

Entry informationi

Entry nameiGLFT1_MYCS2
AccessioniPrimary (citable) accession number: A0R5Z2
Secondary accession number(s): I7FMT0
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 13, 2010
Last sequence update: January 9, 2007
Last modified: June 7, 2017
This is version 62 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families