Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Galactofuranosyl transferase GlfT1

Gene

glfT1

Organism
Mycobacterium smegmatis (strain ATCC 700084 / mc(2)155)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Involved in the biosynthesis of the arabinogalactan (AG) region of the mycolylarabinogalactan-peptidoglycan (mAGP) complex, an essential component the mycobacterial cell wall. It catalyzes the transfer of the first galactofuranosyl (Galf) unit from UDP-galactofuranosyl (UDP-Galf) onto the rhamnosyl-GlcNAc-pyrophosphoryl-undecaprenyl (Rha-GlcNAc-PP-C55) acceptor through a beta-D-(1->5) or a beta-D-(1->6) galactofuranosyltransferase activitie, yielding galactofuranosyl-rhamnosyl-GlcNAc-pyrophosphoryl-undecaprenyl (Galf-Rha-GlcNAc-PP-C55). GlfT1 is also able to catalyze the addition of the second Galf unit.1 Publication

Catalytic activityi

2 UDP-alpha-D-galactofuranose + alpha-L-rhamnopyranosyl-(1->3)-N-acetyl-alpha-D-glucosaminyl-diphospho-trans,octacis-decaprenol = 2 UDP + beta-D-galactofuranosyl-(1->5)-beta-D-galactofuranosyl-(1->4)-alpha-L-rhamnopyranosyl-(1->3)-N-acetyl-alpha-D-glucosaminyl-diphospho-trans,octacis-decaprenol.1 Publication

Pathwayi: cell wall polysaccharide biosynthesis

This protein is involved in the pathway cell wall polysaccharide biosynthesis, which is part of Cell wall biogenesis.
View all proteins of this organism that are known to be involved in the pathway cell wall polysaccharide biosynthesis and in Cell wall biogenesis.

GO - Molecular functioni

  • transferase activity Source: UniProtKB
  • transferase activity, transferring glycosyl groups Source: UniProtKB

GO - Biological processi

  • capsule polysaccharide biosynthetic process Source: UniProtKB-UniPathway
  • cell wall macromolecule biosynthetic process Source: UniProtKB
  • cell wall organization Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Transferase

Keywords - Biological processi

Cell wall biogenesis/degradation

Enzyme and pathway databases

BioCyciMSME246196:GJ4Y-6366-MONOMER.
UniPathwayiUPA00963.

Protein family/group databases

CAZyiGT2. Glycosyltransferase Family 2.

Names & Taxonomyi

Protein namesi
Recommended name:
Galactofuranosyl transferase GlfT1 (EC:2.4.1.287)
Short name:
GalTr
Alternative name(s):
Beta-D-(1->5)galactofuranosyltransferase
Beta-D-(1->6)galactofuranosyltransferase
Rhamnopyranosyl-N-acetylglucosaminyl-diphospho-decaprenol beta-1,3/1,4-galactofuranosyltransferase
UDP-Galf:alpha-3-L-rhamnosyl-alpha-D-GlcNAc-pyrophosphate polyprenol, UDP-galactofuranosyl transferase
Gene namesi
Name:glfT1
Ordered Locus Names:MSMEG_6367, MSMEI_6199
OrganismiMycobacterium smegmatis (strain ATCC 700084 / mc(2)155)
Taxonomic identifieri246196 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaCorynebacterialesMycobacteriaceaeMycobacterium
Proteomesi
  • UP000006158 Componenti: Chromosome
  • UP000000757 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 302302Galactofuranosyl transferase GlfT1PRO_0000395357Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi246196.MSMEG_6367.

Structurei

3D structure databases

ProteinModelPortaliA0R5Z2.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the glycosyltransferase 2 family.Curated

Phylogenomic databases

eggNOGiENOG4108MKI. Bacteria.
COG1216. LUCA.
HOGENOMiHOG000247165.
KOiK16649.
OMAiTCLQTAY.
OrthoDBiEOG6V4GB6.

Family and domain databases

Gene3Di3.90.550.10. 1 hit.
InterProiIPR029044. Nucleotide-diphossugar_trans.
[Graphical view]
SUPFAMiSSF53448. SSF53448. 1 hit.

Sequencei

Sequence statusi: Complete.

A0R5Z2-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTHTEVVCAV VVTHRRRELL ATSLDAVVSQ DRKPDHLIVV DNDNDPQVRE
60 70 80 90 100
LVTGQPVPST YLGSRRNLGG AGGFALGMLH ALALGADWIW LADDDGRPAD
110 120 130 140 150
TTVLSTLLSC AHTHSLAEVS PMVCNLDDPQ RLAFPLRRGL VWRRLTSELR
160 170 180 190 200
TDSSSSSGDL LPGIASLFNG ALFRADTVDA VGVPDLRLFV RGDEVELHRR
210 220 230 240 250
LVRSGLPFGT CLTASYLHPC GTDEFKPILG GRMHTQYPDD ETKRFFTYRN
260 270 280 290 300
RGYLLSQPGL RKLLPQEWLR FGWYFLVSRR DLAGLREWIR LRRLGRRERF

QR
Length:302
Mass (Da):34,041
Last modified:January 9, 2007 - v1
Checksum:i407E1D4CA50DA4BD
GO

Sequence cautioni

The sequence AFP42625.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000480 Genomic DNA. Translation: ABK69689.1.
CP001663 Genomic DNA. Translation: AFP42625.1. Different initiation.
RefSeqiWP_011731236.1. NZ_CP009494.1.
YP_890580.1. NC_008596.1.

Genome annotation databases

EnsemblBacteriaiABK69689; ABK69689; MSMEG_6367.
AFP42625; AFP42625; MSMEI_6199.
GeneIDi4537393.
KEGGimsb:LJ00_31470.
msg:MSMEI_6199.
msm:MSMEG_6367.
PATRICi18084965. VBIMycSme59918_6195.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000480 Genomic DNA. Translation: ABK69689.1.
CP001663 Genomic DNA. Translation: AFP42625.1. Different initiation.
RefSeqiWP_011731236.1. NZ_CP009494.1.
YP_890580.1. NC_008596.1.

3D structure databases

ProteinModelPortaliA0R5Z2.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi246196.MSMEG_6367.

Protein family/group databases

CAZyiGT2. Glycosyltransferase Family 2.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABK69689; ABK69689; MSMEG_6367.
AFP42625; AFP42625; MSMEI_6199.
GeneIDi4537393.
KEGGimsb:LJ00_31470.
msg:MSMEI_6199.
msm:MSMEG_6367.
PATRICi18084965. VBIMycSme59918_6195.

Phylogenomic databases

eggNOGiENOG4108MKI. Bacteria.
COG1216. LUCA.
HOGENOMiHOG000247165.
KOiK16649.
OMAiTCLQTAY.
OrthoDBiEOG6V4GB6.

Enzyme and pathway databases

UniPathwayiUPA00963.
BioCyciMSME246196:GJ4Y-6366-MONOMER.

Family and domain databases

Gene3Di3.90.550.10. 1 hit.
InterProiIPR029044. Nucleotide-diphossugar_trans.
[Graphical view]
SUPFAMiSSF53448. SSF53448. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. Fleischmann R.D., Dodson R.J., Haft D.H., Merkel J.S., Nelson W.C., Fraser C.M.
    Submitted (OCT-2006) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 700084 / mc(2)155.
  2. "Interrupted coding sequences in Mycobacterium smegmatis: authentic mutations or sequencing errors?"
    Deshayes C., Perrodou E., Gallien S., Euphrasie D., Schaeffer C., Van-Dorsselaer A., Poch O., Lecompte O., Reyrat J.-M.
    Genome Biol. 8:R20.1-R20.9(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 700084 / mc(2)155.
  3. "Ortho-proteogenomics: multiple proteomes investigation through orthology and a new MS-based protocol."
    Gallien S., Perrodou E., Carapito C., Deshayes C., Reyrat J.-M., Van Dorsselaer A., Poch O., Schaeffer C., Lecompte O.
    Genome Res. 19:128-135(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 700084 / mc(2)155.
  4. Cited for: FUNCTION AS A GALACTOFURANOSYLTRANSFERASE, CATALYTIC ACTIVITY, SUBSTRATE SPECIFICITY.

Entry informationi

Entry nameiGLFT1_MYCS2
AccessioniPrimary (citable) accession number: A0R5Z2
Secondary accession number(s): I7FMT0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 13, 2010
Last sequence update: January 9, 2007
Last modified: November 11, 2015
This is version 56 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.