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Protein

Acetyl-coenzyme A synthetase

Gene

acsA

Organism
Mycobacterium smegmatis (strain ATCC 700084 / mc(2)155)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the conversion of acetate into acetyl-CoA (AcCoA), an essential intermediate at the junction of anabolic and catabolic pathways. AcsA undergoes a two-step reaction. In the first half reaction, AcsA combines acetate with ATP to form acetyl-adenylate (AcAMP) intermediate. In the second half reaction, it can then transfer the acetyl group from AcAMP to the sulfhydryl group of CoA, forming the product AcCoA.1 Publication

Catalytic activityi

ATP + acetate + CoA = AMP + diphosphate + acetyl-CoA.

Cofactori

Mg2+By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei599 – 5991Coenzyme ABy similarity
Binding sitei796 – 7961ATPBy similarity
Binding sitei811 – 8111ATPBy similarity
Binding sitei819 – 8191Coenzyme A; via carbonyl oxygenBy similarity
Binding sitei822 – 8221ATPBy similarity
Metal bindingi833 – 8331Magnesium; via carbonyl oxygenBy similarity
Metal bindingi835 – 8351Magnesium; via carbonyl oxygenBy similarity
Metal bindingi838 – 8381Magnesium; via carbonyl oxygenBy similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi675 – 6773ATPBy similarity
Nucleotide bindingi699 – 7046ATPBy similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Ligandi

ATP-binding, Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciMSME246196:GJ4Y-6178-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Acetyl-coenzyme A synthetase (EC:6.2.1.1)
Short name:
AcCoA synthetase
Short name:
Acs
Alternative name(s):
Acetate--CoA ligase
Acyl-activating enzyme
Gene namesi
Name:acsA
Ordered Locus Names:MSMEG_6179, MSMEI_6020
OrganismiMycobacterium smegmatis (strain ATCC 700084 / mc(2)155)
Taxonomic identifieri246196 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaCorynebacterialesMycobacteriaceaeMycobacterium
Proteomesi
  • UP000006158 Componenti: Chromosome
  • UP000000757 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 3333Sequence analysisAdd
BLAST
Chaini34 – 940907Acetyl-coenzyme A synthetasePRO_0000420242Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei906 – 9061N6-acetyllysineBy similarity

Post-translational modificationi

Acetylated on Lys-906 by Pat in the presence of acetyl-CoA as an acetyl donor and ATP. Acetylation results in the inactivation of the enzyme. Deacetylation by the SIR2-homolog deacetylase CobB is required to activate the enzyme (By similarity).By similarity

Keywords - PTMi

Acetylation

Interactioni

Protein-protein interaction databases

STRINGi246196.MSMEG_6179.

Structurei

3D structure databases

ProteinModelPortaliA0R5G1.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1 – 289289UnknownAdd
BLAST
Regioni290 – 940651Acetyl-coenzyme A synthetaseAdd
BLAST
Regioni480 – 4834Coenzyme A bindingBy similarity

Sequence similaritiesi

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiENOG4108IQF. Bacteria.
COG0365. LUCA.
HOGENOMiHOG000229981.
KOiK01895.
OMAiMMENRDL.
OrthoDBiEOG68WR2H.

Family and domain databases

HAMAPiMF_01123. Ac_CoA_synth.
InterProiIPR011904. Ac_CoA_lig.
IPR032387. ACAS_N.
IPR025110. AMP-bd_C.
IPR020845. AMP-binding_CS.
IPR000873. AMP-dep_Synth/Lig.
[Graphical view]
PfamiPF16177. ACAS_N. 1 hit.
PF00501. AMP-binding. 1 hit.
PF13193. AMP-binding_C. 1 hit.
[Graphical view]
TIGRFAMsiTIGR02188. Ac_CoA_lig_AcsA. 1 hit.
PROSITEiPS00455. AMP_BINDING. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

A0R5G1-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MCCAIWSASR APACSASQLS SSHAVRPSVV PDANPRAAPR YSPDLRIQCT
60 70 80 90 100
SGTPRPCGSS PLAIVTSRET ARATTPSAVP NGASVSNSPC ATAASTGAGA
110 120 130 140 150
LDPPVRSSIS NSRRGPTSGS VRGRPRCSSS VRSAAASACS RASACIGARC
160 170 180 190 200
HTVTNEVAPP SSASATRIAP AVTGSGSPSA DSCTAPPSST ADARAPRSAD
210 220 230 240 250
SAAVCGQPRR LTLCRCARPS ASRASSQAAA TPGSSNSGAR GSGRSVITSH
260 270 280 290 300
ASRPLIASSP TVQRNQTTVH QQTCEGPTCV QRSVTRLTAM SNPSHAEVPS
310 320 330 340 350
AYPPPADFAA NANATGELYA EAEKDRLAFW EKQAKRLSWQ TPFTDVLDWS
360 370 380 390 400
DAPFAKWFVG GKINVAYNCV DRHVEAGNGD RVAIHWEGEP VGDARSITYA
410 420 430 440 450
ELKDEVCKAA NALTDLGLVA GDRVAIYMPM IPEAIVAMLA CARLGVMHSV
460 470 480 490 500
VFAGFSASAL RARIEDAEAK LVITSDGQYR RGKAASLKEA VDEAVADQPS
510 520 530 540 550
VKNVLVVRRT GIDVKWTDGR DLWWDETVEQ ASTEHIPAAF DSEQPLFLLY
560 570 580 590 600
TSGTTGKPKG IVHTSGGYLT QSSYTHWNVF DVKPESDVYW CTADIGWVTG
610 620 630 640 650
HTYIVYGPLS NGVTQVVYEG TPTSPTEHRH FEVIEKYGVT IYYTAPTLIR
660 670 680 690 700
TFMKLGHQIP ASHDLSSLRL LGSVGEPINP EAWRWYREHI GGGKTPIVDT
710 720 730 740 750
WWQTETGAIM ISPLPGVTAA KPGSAMTPLP GISAKIVDDE GNQLVPGADE
760 770 780 790 800
AEHVTGYLVL DQPWPAMLRG IWGDPQRFKD TYWSRFAEQG WYFAGDGARY
810 820 830 840 850
DSDGHIWVLG RIDDVMNVSG HRISTAEVES ALVGHAGVAE AAVVGASDDT
860 870 880 890 900
TGQAICAFVI LKASAHGGPE NMIEELRAEV AREISPIAKP REIHIVPELP
910 920 930 940
KTRSGKIMRR LLRDVAEGRE LGDTSTLVDP SVFEAIRASK
Length:940
Mass (Da):99,957
Last modified:January 9, 2007 - v1
Checksum:i18AB5298FB108F45
GO

Sequence cautioni

The sequence AFP42452.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000480 Genomic DNA. Translation: ABK69538.1.
CP001663 Genomic DNA. Translation: AFP42452.1. Different initiation.
RefSeqiWP_011731097.1. NC_008596.1.
YP_890399.1. NC_008596.1.

Genome annotation databases

EnsemblBacteriaiABK69538; ABK69538; MSMEG_6179.
AFP42452; AFP42452; MSMEI_6020.
GeneIDi4534714.
KEGGimsg:MSMEI_6020.
msm:MSMEG_6179.
PATRICi18084601. VBIMycSme59918_6019.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000480 Genomic DNA. Translation: ABK69538.1.
CP001663 Genomic DNA. Translation: AFP42452.1. Different initiation.
RefSeqiWP_011731097.1. NC_008596.1.
YP_890399.1. NC_008596.1.

3D structure databases

ProteinModelPortaliA0R5G1.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi246196.MSMEG_6179.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABK69538; ABK69538; MSMEG_6179.
AFP42452; AFP42452; MSMEI_6020.
GeneIDi4534714.
KEGGimsg:MSMEI_6020.
msm:MSMEG_6179.
PATRICi18084601. VBIMycSme59918_6019.

Phylogenomic databases

eggNOGiENOG4108IQF. Bacteria.
COG0365. LUCA.
HOGENOMiHOG000229981.
KOiK01895.
OMAiMMENRDL.
OrthoDBiEOG68WR2H.

Enzyme and pathway databases

BioCyciMSME246196:GJ4Y-6178-MONOMER.

Family and domain databases

HAMAPiMF_01123. Ac_CoA_synth.
InterProiIPR011904. Ac_CoA_lig.
IPR032387. ACAS_N.
IPR025110. AMP-bd_C.
IPR020845. AMP-binding_CS.
IPR000873. AMP-dep_Synth/Lig.
[Graphical view]
PfamiPF16177. ACAS_N. 1 hit.
PF00501. AMP-binding. 1 hit.
PF13193. AMP-binding_C. 1 hit.
[Graphical view]
TIGRFAMsiTIGR02188. Ac_CoA_lig_AcsA. 1 hit.
PROSITEiPS00455. AMP_BINDING. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. Fleischmann R.D., Dodson R.J., Haft D.H., Merkel J.S., Nelson W.C., Fraser C.M.
    Submitted (OCT-2006) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 700084 / mc(2)155.
  2. "Interrupted coding sequences in Mycobacterium smegmatis: authentic mutations or sequencing errors?"
    Deshayes C., Perrodou E., Gallien S., Euphrasie D., Schaeffer C., Van-Dorsselaer A., Poch O., Lecompte O., Reyrat J.-M.
    Genome Biol. 8:R20.1-R20.9(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 700084 / mc(2)155.
  3. "Ortho-proteogenomics: multiple proteomes investigation through orthology and a new MS-based protocol."
    Gallien S., Perrodou E., Carapito C., Deshayes C., Reyrat J.-M., Van Dorsselaer A., Poch O., Schaeffer C., Lecompte O.
    Genome Res. 19:128-135(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 700084 / mc(2)155.
  4. "Reversible acetylation and inactivation of Mycobacterium tuberculosis acetyl-CoA synthetase is dependent on cAMP."
    Xu H., Hegde S.S., Blanchard J.S.
    Biochemistry 50:5883-5892(2011) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.

Entry informationi

Entry nameiACSA_MYCS2
AccessioniPrimary (citable) accession number: A0R5G1
Secondary accession number(s): I7GA85
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 28, 2012
Last sequence update: January 9, 2007
Last modified: July 6, 2016
This is version 73 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.