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Protein

Acetyl-coenzyme A synthetase

Gene

acsA

Organism
Mycobacterium smegmatis (strain ATCC 700084 / mc(2)155)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the conversion of acetate into acetyl-CoA (AcCoA), an essential intermediate at the junction of anabolic and catabolic pathways. AcsA undergoes a two-step reaction. In the first half reaction, AcsA combines acetate with ATP to form acetyl-adenylate (AcAMP) intermediate. In the second half reaction, it can then transfer the acetyl group from AcAMP to the sulfhydryl group of CoA, forming the product AcCoA.1 Publication

Catalytic activityi

ATP + acetate + CoA = AMP + diphosphate + acetyl-CoA.

Cofactori

Mg2+By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei599Coenzyme ABy similarity1
Binding sitei796ATPBy similarity1
Binding sitei811ATPBy similarity1
Binding sitei819Coenzyme A; via carbonyl oxygenBy similarity1
Binding sitei822ATPBy similarity1
Metal bindingi833Magnesium; via carbonyl oxygenBy similarity1
Metal bindingi835Magnesium; via carbonyl oxygenBy similarity1
Metal bindingi838Magnesium; via carbonyl oxygenBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi675 – 677ATPBy similarity3
Nucleotide bindingi699 – 704ATPBy similarity6

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Ligandi

ATP-binding, Magnesium, Metal-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Acetyl-coenzyme A synthetase (EC:6.2.1.1)
Short name:
AcCoA synthetase
Short name:
Acs
Alternative name(s):
Acetate--CoA ligase
Acyl-activating enzyme
Gene namesi
Name:acsA
Ordered Locus Names:MSMEG_6179, MSMEI_6020
OrganismiMycobacterium smegmatis (strain ATCC 700084 / mc(2)155)
Taxonomic identifieri246196 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaCorynebacterialesMycobacteriaceaeMycobacterium
Proteomesi
  • UP000006158 Componenti: Chromosome
  • UP000000757 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 33Sequence analysisAdd BLAST33
ChainiPRO_000042024234 – 940Acetyl-coenzyme A synthetaseAdd BLAST907

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei906N6-acetyllysineBy similarity1

Post-translational modificationi

Acetylated on Lys-906 by Pat in the presence of acetyl-CoA as an acetyl donor and ATP. Acetylation results in the inactivation of the enzyme. Deacetylation by the SIR2-homolog deacetylase CobB is required to activate the enzyme (By similarity).By similarity

Keywords - PTMi

Acetylation

Interactioni

Protein-protein interaction databases

STRINGi246196.MSMEG_6179.

Structurei

3D structure databases

ProteinModelPortaliA0R5G1.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1 – 289UnknownAdd BLAST289
Regioni290 – 940Acetyl-coenzyme A synthetaseAdd BLAST651
Regioni480 – 483Coenzyme A bindingBy similarity4

Sequence similaritiesi

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiENOG4108IQF. Bacteria.
COG0365. LUCA.
HOGENOMiHOG000229981.
KOiK01895.
OMAiNTHIEAT.
OrthoDBiPOG091H059D.

Family and domain databases

CDDicd05966. ACS. 1 hit.
HAMAPiMF_01123. Ac_CoA_synth. 1 hit.
InterProiIPR011904. Ac_CoA_lig.
IPR032387. ACAS_N.
IPR025110. AMP-bd_C.
IPR020845. AMP-binding_CS.
IPR000873. AMP-dep_Synth/Lig.
[Graphical view]
PfamiPF16177. ACAS_N. 1 hit.
PF00501. AMP-binding. 1 hit.
PF13193. AMP-binding_C. 1 hit.
[Graphical view]
TIGRFAMsiTIGR02188. Ac_CoA_lig_AcsA. 1 hit.
PROSITEiPS00455. AMP_BINDING. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

A0R5G1-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MCCAIWSASR APACSASQLS SSHAVRPSVV PDANPRAAPR YSPDLRIQCT
60 70 80 90 100
SGTPRPCGSS PLAIVTSRET ARATTPSAVP NGASVSNSPC ATAASTGAGA
110 120 130 140 150
LDPPVRSSIS NSRRGPTSGS VRGRPRCSSS VRSAAASACS RASACIGARC
160 170 180 190 200
HTVTNEVAPP SSASATRIAP AVTGSGSPSA DSCTAPPSST ADARAPRSAD
210 220 230 240 250
SAAVCGQPRR LTLCRCARPS ASRASSQAAA TPGSSNSGAR GSGRSVITSH
260 270 280 290 300
ASRPLIASSP TVQRNQTTVH QQTCEGPTCV QRSVTRLTAM SNPSHAEVPS
310 320 330 340 350
AYPPPADFAA NANATGELYA EAEKDRLAFW EKQAKRLSWQ TPFTDVLDWS
360 370 380 390 400
DAPFAKWFVG GKINVAYNCV DRHVEAGNGD RVAIHWEGEP VGDARSITYA
410 420 430 440 450
ELKDEVCKAA NALTDLGLVA GDRVAIYMPM IPEAIVAMLA CARLGVMHSV
460 470 480 490 500
VFAGFSASAL RARIEDAEAK LVITSDGQYR RGKAASLKEA VDEAVADQPS
510 520 530 540 550
VKNVLVVRRT GIDVKWTDGR DLWWDETVEQ ASTEHIPAAF DSEQPLFLLY
560 570 580 590 600
TSGTTGKPKG IVHTSGGYLT QSSYTHWNVF DVKPESDVYW CTADIGWVTG
610 620 630 640 650
HTYIVYGPLS NGVTQVVYEG TPTSPTEHRH FEVIEKYGVT IYYTAPTLIR
660 670 680 690 700
TFMKLGHQIP ASHDLSSLRL LGSVGEPINP EAWRWYREHI GGGKTPIVDT
710 720 730 740 750
WWQTETGAIM ISPLPGVTAA KPGSAMTPLP GISAKIVDDE GNQLVPGADE
760 770 780 790 800
AEHVTGYLVL DQPWPAMLRG IWGDPQRFKD TYWSRFAEQG WYFAGDGARY
810 820 830 840 850
DSDGHIWVLG RIDDVMNVSG HRISTAEVES ALVGHAGVAE AAVVGASDDT
860 870 880 890 900
TGQAICAFVI LKASAHGGPE NMIEELRAEV AREISPIAKP REIHIVPELP
910 920 930 940
KTRSGKIMRR LLRDVAEGRE LGDTSTLVDP SVFEAIRASK
Length:940
Mass (Da):99,957
Last modified:January 9, 2007 - v1
Checksum:i18AB5298FB108F45
GO

Sequence cautioni

The sequence AFP42452 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000480 Genomic DNA. Translation: ABK69538.1.
CP001663 Genomic DNA. Translation: AFP42452.1. Different initiation.
RefSeqiWP_011731097.1. NC_008596.1.
WP_014878580.1. NZ_CP009494.1.
YP_890399.1. NC_008596.1.

Genome annotation databases

EnsemblBacteriaiABK69538; ABK69538; MSMEG_6179.
AFP42452; AFP42452; MSMEI_6020.
GeneIDi4534714.
KEGGimsg:MSMEI_6020.
msm:MSMEG_6179.
PATRICi18084601. VBIMycSme59918_6019.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000480 Genomic DNA. Translation: ABK69538.1.
CP001663 Genomic DNA. Translation: AFP42452.1. Different initiation.
RefSeqiWP_011731097.1. NC_008596.1.
WP_014878580.1. NZ_CP009494.1.
YP_890399.1. NC_008596.1.

3D structure databases

ProteinModelPortaliA0R5G1.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi246196.MSMEG_6179.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABK69538; ABK69538; MSMEG_6179.
AFP42452; AFP42452; MSMEI_6020.
GeneIDi4534714.
KEGGimsg:MSMEI_6020.
msm:MSMEG_6179.
PATRICi18084601. VBIMycSme59918_6019.

Phylogenomic databases

eggNOGiENOG4108IQF. Bacteria.
COG0365. LUCA.
HOGENOMiHOG000229981.
KOiK01895.
OMAiNTHIEAT.
OrthoDBiPOG091H059D.

Family and domain databases

CDDicd05966. ACS. 1 hit.
HAMAPiMF_01123. Ac_CoA_synth. 1 hit.
InterProiIPR011904. Ac_CoA_lig.
IPR032387. ACAS_N.
IPR025110. AMP-bd_C.
IPR020845. AMP-binding_CS.
IPR000873. AMP-dep_Synth/Lig.
[Graphical view]
PfamiPF16177. ACAS_N. 1 hit.
PF00501. AMP-binding. 1 hit.
PF13193. AMP-binding_C. 1 hit.
[Graphical view]
TIGRFAMsiTIGR02188. Ac_CoA_lig_AcsA. 1 hit.
PROSITEiPS00455. AMP_BINDING. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiACSA_MYCS2
AccessioniPrimary (citable) accession number: A0R5G1
Secondary accession number(s): I7GA85
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 28, 2012
Last sequence update: January 9, 2007
Last modified: November 30, 2016
This is version 77 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.