A0R5D9 (A0R5D9_MYCS2) Unreviewed, UniProtKB/TrEMBL

Names and origin

Protein names

Submitted name:
DNA topoisomerase I EMBL ABK69586.1
EC=5.99.1.2 EMBL ABK69586.1

Gene names

Name:	topA EMBL ABK69586.1
Ordered Locus Names:	MSMEG_6157

Organism

Mycobacterium smegmatis (strain ATCC 700084 / mc(2)155) EMBL ABK69586.1

Taxonomic identifier

246196 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Corynebacterineae › Mycobacteriaceae › Mycobacterium

Protein attributes

Sequence length	936 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Releases the supercoiling and torsional tension of DNA introduced during the DNA replication and transcription by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(5'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 3'-OH DNA strand. The free DNA strand than undergoes passage around the unbroken strand thus removing DNA supercoils. Finally, in the religation step, the DNA 3'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone By similarity. SAAS SAAS000380
Catalytic activity	ATP-independent breakage of single-stranded DNA, followed by passage and rejoining. SAAS SAAS005733
Sequence similarities	Contains 1 Toprim domain. SAAS SAAS023406

Ontologies

Keywords
Ligand	ATP-binding SAAS SAAS005733 DNA-binding SAAS SAAS005733 Nucleotide-binding
Molecular function	Isomerase Topoisomerase SAAS SAAS005733
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	DNA topological change Inferred from electronic annotation. Source: InterPro
Cellular component	chromosome Inferred from electronic annotation. Source: InterPro
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW DNA topoisomerase type I activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequences

	Sequence		Length	Mass (Da)
	A0R5D9 [UniParc]. Last modified January 9, 2007. Version 1. Checksum: CCFB66C8A9AD3F22	FASTA	936	102,499
	10 20 30 40 50 60 MAGGDRGSGG TGNVRRLVIV ESPTKARKIA GYLGSNYVVE SSRGHIRDLP RNAADVPAKF 70 80 90 100 110 120 KSEPWARLGV NVDQNFEPLY IVSPEKKSTV TELKGLLKDV DELYLATDGD REGEAIAWHL 130 140 150 160 170 180 LETLKPRVPV KRMVFHEITE PAIRNAAENP RDLDIALVDA QETRRILDRL YGYEVSPVLW 190 200 210 220 230 240 KKVAPKLSAG RVQSVATRII VQRERERMAF HSASYWDVTA ELDASVSDPS ASPPKFTAKL 250 260 270 280 290 300 NTVDGRRVAT GRDFDSLGQL KRPDEVLVLD EASAGALASG LRGAQLAVTS VEQKPYTRRP 310 320 330 340 350 360 YAPFMTSTLQ QEAARKLRFS SERTMSIAQR LYENGYITYM RTDSTTLSES AINAARTQAR 370 380 390 400 410 420 QLYGEEYVHP SPRQYTRKVK NAQEAHEAIR PAGDVFQTPG QLHSALDTDE FRLYELIWQR 430 440 450 460 470 480 TVASQMADAR GTTLSLRIGG SASSGEQVVF NASGRTITFP GFLKAYVESI DELAGGESDD 490 500 510 520 530 540 AESRLPNLTQ GQRVDAADLS ADGHQTSPPA RYTEASLIKA LEELGIGRPS TYSSIIKTIQ 550 560 570 580 590 600 DRGYVQKKGS ALVPSWVAFA VVGLLEQHFG RLVDYDFTAA MEDELDEIAN GQEQRTNWLN 610 620 630 640 650 660 NFYFGGEHGV EGSIARAGGL KQLVGGNLEG IDAREVNSIK VFDDSEGRPV YVRVGRNGPY 670 680 690 700 710 720 LERMVDDPDN PGEQKPQRAN LKEDLTPDEL TPELAEKLFA TPQEGRSLGI DPETGHEIVA 730 740 750 760 770 780 KDGRFGPYVT EVLPEPEDGG DDGTAGTPAK KGKKPTGPKP RTGSLFRSMD LETVTLEDAL 790 800 810 820 830 840 KLLSLPRVVG VDPTTNEEIT AQNGRYGPYL KRGTDSRSLA TEDQIFTITL DEALKIYAEP 850 860 870 880 890 900 KRRGRQAASA PPLRELGNDP VSGKPMVIKD GRFGPYVTDG ETNASLRKGD DVLTITDERA 910 920 930 SELLADRRAR GPVKKKAPAK KAAKKAPAKK AAAKKA

References

[1]	Fleischmann R.D., Dodson R.J., Haft D.H., Merkel J.S., Nelson W.C., Fraser C.M. Submitted (OCT-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 700084 / mc(2)155.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000480 Genomic DNA. Translation: ABK69586.1.
RefSeq	YP_890377.1. NC_008596.1.
3D structure databases
ProteinModelPortal	A0R5D9.
ModBase	Search...
Protein-protein interaction databases
STRING	A0R5D9.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBMYCT00000041803; EBMYCP00000040140; EBMYCG00000041798.
GeneID	4535825.
GenomeReviews	Gene locus MSMEG_6157 in contig CP000480_GR.
KEGG	msm:MSMEG_6157.
NMPDR	fig|246196.1.peg.6120.
PATRIC	18084555. VBIMycSme59918_5996.
TIGR	MSMEG_6157.
Phylogenomic databases
eggNOG	COG0550.
GeneTree	EBGT00050000016895.
HOGENOM	HBG512935.
OMA	KKVMPRL.
PhylomeDB	A0R5D9.
ProtClustDB	PRK07561.
Family and domain databases
InterPro	IPR000380. Topo_IA. IPR003601. Topo_IA_2. IPR023406. Topo_IA_AS. IPR013497. Topo_IA_cen. IPR013824. Topo_IA_cen_sub1. IPR013825. Topo_IA_cen_sub2. IPR023405. Topo_IA_core_domain. IPR003602. Topo_IA_DNA-bd. IPR005733. TopoI_bac-type. IPR006171. Toprim_domain. [Graphical view]
Gene3D	G3DSA:1.10.460.10. Topo_IA_cen_sub1. 2 hits. G3DSA:2.70.20.10. Topo_IA_cen_sub2. 2 hits.
KO	K03168.
PANTHER	PTHR11390. Topo_IA. 1 hit.
Pfam	PF01131. Topoisom_bac. 1 hit. PF01751. Toprim. 1 hit. [Graphical view]
PRINTS	PR00417. PRTPISMRASEI.
SMART	SM00437. TOP1Ac. 1 hit. SM00436. TOP1Bc. 1 hit. SM00493. TOPRIM. 1 hit. [Graphical view]
SUPFAM	SSF56712. Topo_IA_core. 1 hit.
TIGRFAMs	TIGR01051. TopA_bact. 1 hit.
PROSITE	PS00396. TOPOISOMERASE_I_PROK. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

A0R5D9_MYCS2

Accession

Primary (citable) accession number: A0R5D9

Entry history

Integrated into UniProtKB/TrEMBL:	January 9, 2007
Last sequence update:	January 9, 2007
Last modified:	January 25, 2012
This is version 50 of the entry and version 1 of the sequence. [Complete history]

Entry status

Unreviewed (UniProtKB/TrEMBL)