A0R580 (PANC_MYCS2) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Pantothenate synthetase
Short name=PS
EC=6.3.2.1

Alternative name(s):
Pantoate--beta-alanine ligase
Pantoate-activating enzyme

Gene names

Name:	panC
Ordered Locus Names:	MSMEG_6097, MSMEI_5938

Organism

Mycobacterium smegmatis (strain ATCC 700084 / mc(2)155) [Reference proteome] [HAMAP]

Taxonomic identifier

246196 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Actinobacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Corynebacterineae › Mycobacteriaceae › Mycobacterium › Mycobacterium smegmatis

Protein attributes

Sequence length	314 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Catalyzes the condensation of pantoate with beta-alanine in an ATP-dependent reaction via a pantoyl-adenylate intermediate By similarity. HAMAP-Rule MF_00158
Catalytic activity	ATP + (R)-pantoate + beta-alanine = AMP + diphosphate + (R)-pantothenate. HAMAP-Rule MF_00158
Pathway	Cofactor biosynthesis; (R)-pantothenate biosynthesis; (R)-pantothenate from (R)-pantoate and beta-alanine: step 1/1. HAMAP-Rule MF_00158
Subunit structure	Homodimer By similarity. HAMAP-Rule MF_00158
Subcellular location	Cytoplasm Potential HAMAP-Rule MF_00158.
Miscellaneous	The reaction proceeds by a bi uni uni bi ping pong mechanism By similarity. HAMAP-Rule MF_00158
Sequence similarities	Belongs to the pantothenate synthetase family.

Ontologies

Keywords
Biological process	Pantothenate biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	pantothenate biosynthetic process Inferred from electronic annotation. Source: HAMAP
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW pantoate-beta-alanine ligase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 314	314	Pantothenate synthetase HAMAP-Rule MF_00158		PRO_0000305487
Regions
	Nucleotide binding	43 – 50	8	ATP By similarity
	Nucleotide binding	161 – 164	4	ATP By similarity
	Nucleotide binding	198 – 201	4	ATP By similarity
Sites
	Active site	50	1	Proton donor By similarity
	Binding site	75	1	Beta-alanine By similarity
	Binding site	75	1	Pantoate By similarity
	Binding site	167	1	Pantoate By similarity
	Binding site	190	1	ATP; via amide nitrogen and carbonyl oxygen By similarity

Sequences

	Sequence		Length	Mass (Da)
	A0R580 [UniParc]. Last modified January 9, 2007. Version 1. Checksum: AABE76E46E7E96AF	FASTA	314	33,525
	10 20 30 40 50 60 MTISRTPKFS AGELNVYSAP ADVAAVTRAL RTAGRRIVLV PTMGALHEGH LTLVRAAKRT 70 80 90 100 110 120 PGAVVVVSIF VNPLQFGPNE DLNAYPRTLE DDLTALRAEG VEIVFTPTGS DMYPDGTRTS 130 140 150 160 170 180 VHPGPLGDDL EGSSRPGHFA GVLTVVLKLF SIVRPDRAYF GEKDYQQLTL LRQMVADLNV 190 200 210 220 230 240 DVQIVGVPTV RESDGLALSS RNRYLDKDQR EQAGALSAAL LAGKYAAAGG AEAALDAARA 250 260 270 280 290 300 VLDEVPALEV DYLQVRDPML GPAPAEGQAR LLVAARLGRT RLIDNIAIDV GASAGIDGHP 310 RVGNDQNHEL PWRN

References

[1]	Fleischmann R.D., Dodson R.J., Haft D.H., Merkel J.S., Nelson W.C., Fraser C.M. Submitted (OCT-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 700084 / mc(2)155.
[2]	"Interrupted coding sequences in Mycobacterium smegmatis: authentic mutations or sequencing errors?" Deshayes C., Perrodou E., Gallien S., Euphrasie D., Schaeffer C., Van-Dorsselaer A., Poch O., Lecompte O., Reyrat J.-M. Genome Biol. 8:R20.1-R20.9(2007) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 700084 / mc(2)155.
[3]	"Ortho-proteogenomics: multiple proteomes investigation through orthology and a new MS-based protocol." Gallien S., Perrodou E., Carapito C., Deshayes C., Reyrat J.-M., Van Dorsselaer A., Poch O., Schaeffer C., Lecompte O. Genome Res. 19:128-135(2009) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]. Strain: ATCC 700084 / mc(2)155.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000480 Genomic DNA. Translation: ABK70073.1. CP001663 Genomic DNA. Translation: AFP42371.1.
RefSeq	YP_006570666.1. NC_018289.1. YP_890318.1. NC_008596.1.
3D structure databases
ProteinModelPortal	A0R580.
SMR	A0R580. Positions 7-294.
ModBase	Search...
Protein-protein interaction databases
STRING	246196.MSMEG_6097.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	ABK70073; ABK70073; MSMEG_6097.
GeneID	13427268. 4531246.
KEGG	msg:MSMEI_5938. msm:MSMEG_6097.
PATRIC	18084433. VBIMycSme59918_5936.
Phylogenomic databases
eggNOG	COG0414.
HOGENOM	HOG000175516.
KO	K01918.
OMA	EDFGSYP.
ProtClustDB	PRK00380.
Enzyme and pathway databases
BioCyc	MSME246196:GJ4Y-6096-MONOMER.
UniPathway	UPA00028; UER00005.
Family and domain databases
Gene3D	3.40.50.620. 1 hit.
HAMAP	MF_00158. PanC.
InterPro	IPR003721. Pantoate_ligase. IPR014729. Rossmann-like_a/b/a_fold. [Graphical view]
PANTHER	PTHR21299:SF1. PTHR21299:SF1. 1 hit.
Pfam	PF02569. Pantoate_ligase. 1 hit. [Graphical view]
TIGRFAMs	TIGR00018. panC. 1 hit.
ProtoNet	Search...

Entry information

Entry name

PANC_MYCS2

Accession

Primary (citable) accession number: A0R580
Secondary accession number(s): I7GFF1

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 2, 2007
Last sequence update:	January 9, 2007
Last modified:	May 1, 2013
This is version 48 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program