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Protein

DNA repair protein RadA

Gene

radA

Organism
Mycobacterium smegmatis (strain ATCC 700084 / mc(2)155)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

DNA-dependent ATPase involved in processing of recombination intermediates, plays a role in repairing DNA breaks. Stimulates the branch migration of RecA-mediated strand transfer reactions, allowing the 3' invading strand to extend heteroduplex DNA faster. Binds ssDNA in the presence of ADP but not other nucleotides, has ATPase activity that is stimulated by ssDNA and various branched DNA structures, but inhibited by SSB. Does not have RecA's homology-searching function (By similarity). Also inhibits the diadenylate cyclase activity of DisA (PubMed:23760274).UniRule annotation1 Publication

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri12 – 2918C4-typeUniRule annotationAdd
BLAST
Nucleotide bindingi100 – 1078ATPUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

DNA damage, DNA repair, Stress response

Keywords - Ligandi

ATP-binding, DNA-binding, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

BioCyciMSME246196:GJ4Y-6078-MONOMER.

Protein family/group databases

MEROPSiS16.004.

Names & Taxonomyi

Protein namesi
Recommended name:
DNA repair protein RadAUniRule annotation (EC:3.6.4.-UniRule annotation)
Alternative name(s):
Branch migration protein RadAUniRule annotation
Gene namesi
Name:radAUniRule annotation
Ordered Locus Names:MSMEG_6079, MSMEI_5919
OrganismiMycobacterium smegmatis (strain ATCC 700084 / mc(2)155)
Taxonomic identifieri246196 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaCorynebacterialesMycobacteriaceaeMycobacterium
Proteomesi
  • UP000006158 Componenti: Chromosome
  • UP000000757 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 466466DNA repair protein RadAPRO_0000424182Add
BLAST

Expressioni

Inductioni

Forms part of an operon with disA.1 Publication

Interactioni

Subunit structurei

Interacts with DisA.1 Publication

Protein-protein interaction databases

STRINGi246196.MSMEG_6079.

Structurei

3D structure databases

ProteinModelPortaliA0R563.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni359 – 466108Lon-protease-likeUniRule annotationAdd
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi261 – 2655RadA KNRFG motifUniRule annotation

Domaini

Has a putative N-terminal zinc-finger, a middle region with homology to RecA with ATPase motifs including the RadA KNRFG motif, while the C-terminus is homologous to Lon protease.UniRule annotation

Sequence similaritiesi

Belongs to the RecA family. RadA subfamily.UniRule annotation

Zinc finger

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri12 – 2918C4-typeUniRule annotationAdd
BLAST

Keywords - Domaini

Zinc-finger

Phylogenomic databases

eggNOGiENOG4105DNJ. Bacteria.
COG1066. LUCA.
HOGENOMiHOG000218329.
KOiK04485.
OMAiFEGERGH.
OrthoDBiEOG6DRPHC.

Family and domain databases

Gene3Di3.30.230.10. 1 hit.
3.40.50.300. 1 hit.
HAMAPiMF_01498. RadA_bact.
InterProiIPR003593. AAA+_ATPase.
IPR004504. DNA_repair_RadA.
IPR027417. P-loop_NTPase.
IPR020588. RecA_ATP-bd.
IPR020568. Ribosomal_S5_D2-typ_fold.
IPR014721. Ribosomal_S5_D2-typ_fold_subgr.
[Graphical view]
PRINTSiPR01874. DNAREPAIRADA.
SMARTiSM00382. AAA. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
SSF54211. SSF54211. 2 hits.
TIGRFAMsiTIGR00416. sms. 1 hit.
PROSITEiPS50162. RECA_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

A0R563-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAGSKIRSQY RCSECQHVAP KWVGRCANCG TWGTVDEVAV LAGNNKLNGA
60 70 80 90 100
ARRSVAPTSP AVPITSIDPG VTRHYPTGVS ELDRVLGGGL VAGSVTLLAG
110 120 130 140 150
DPGVGKSTLL LEVANRWAHS GKRALYLSGE ESAGQIRLRA ERTGCTHDQV
160 170 180 190 200
YLAAESDLQI ALGHIDEVKP SLVVVDSVQT MSTTEADGVT GGVTQVRAVT
210 220 230 240 250
TSLTAYAKAA VGDPAVAMIL VGHVTKDGAI AGPRSLEHLV DVVLHFEGDR
260 270 280 290 300
ASSLRMVRGV KNRFGAADEV GCFQLHDNGI ECVSDPSGLF LDQRPLAVPG
310 320 330 340 350
TAVTVTLDGK RPMIGEVQAL VSPPAGPPRR AVSGIDSARA AMIGAVLQTR
360 370 380 390 400
CRMPINSNDL YLSTVGGMRL TDPSADLAVA LAIASAYFDI AMPMKAIAIG
410 420 430 440 450
EVGLAGDLRR VTGMDRRLSE AARLGFTTAV VPPGVTSAPA GLKVVAADNI
460
RAAVQTMREI AIAGAQ
Length:466
Mass (Da):48,332
Last modified:January 9, 2007 - v1
Checksum:i231581C9A80EBB15
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000480 Genomic DNA. Translation: ABK69709.1.
CP001663 Genomic DNA. Translation: AFP42352.1.
RefSeqiWP_011731022.1. NZ_CP009494.1.
YP_890301.1. NC_008596.1.

Genome annotation databases

EnsemblBacteriaiABK69709; ABK69709; MSMEG_6079.
AFP42352; AFP42352; MSMEI_5919.
GeneIDi4535537.
KEGGimsb:LJ00_30060.
msg:MSMEI_5919.
msm:MSMEG_6079.
PATRICi18084395. VBIMycSme59918_5917.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000480 Genomic DNA. Translation: ABK69709.1.
CP001663 Genomic DNA. Translation: AFP42352.1.
RefSeqiWP_011731022.1. NZ_CP009494.1.
YP_890301.1. NC_008596.1.

3D structure databases

ProteinModelPortaliA0R563.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi246196.MSMEG_6079.

Protein family/group databases

MEROPSiS16.004.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABK69709; ABK69709; MSMEG_6079.
AFP42352; AFP42352; MSMEI_5919.
GeneIDi4535537.
KEGGimsb:LJ00_30060.
msg:MSMEI_5919.
msm:MSMEG_6079.
PATRICi18084395. VBIMycSme59918_5917.

Phylogenomic databases

eggNOGiENOG4105DNJ. Bacteria.
COG1066. LUCA.
HOGENOMiHOG000218329.
KOiK04485.
OMAiFEGERGH.
OrthoDBiEOG6DRPHC.

Enzyme and pathway databases

BioCyciMSME246196:GJ4Y-6078-MONOMER.

Family and domain databases

Gene3Di3.30.230.10. 1 hit.
3.40.50.300. 1 hit.
HAMAPiMF_01498. RadA_bact.
InterProiIPR003593. AAA+_ATPase.
IPR004504. DNA_repair_RadA.
IPR027417. P-loop_NTPase.
IPR020588. RecA_ATP-bd.
IPR020568. Ribosomal_S5_D2-typ_fold.
IPR014721. Ribosomal_S5_D2-typ_fold_subgr.
[Graphical view]
PRINTSiPR01874. DNAREPAIRADA.
SMARTiSM00382. AAA. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
SSF54211. SSF54211. 2 hits.
TIGRFAMsiTIGR00416. sms. 1 hit.
PROSITEiPS50162. RECA_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. Fleischmann R.D., Dodson R.J., Haft D.H., Merkel J.S., Nelson W.C., Fraser C.M.
    Submitted (OCT-2006) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 700084 / mc(2)155.
  2. "Interrupted coding sequences in Mycobacterium smegmatis: authentic mutations or sequencing errors?"
    Deshayes C., Perrodou E., Gallien S., Euphrasie D., Schaeffer C., Van-Dorsselaer A., Poch O., Lecompte O., Reyrat J.-M.
    Genome Biol. 8:R20.1-R20.9(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 700084 / mc(2)155.
  3. "Ortho-proteogenomics: multiple proteomes investigation through orthology and a new MS-based protocol."
    Gallien S., Perrodou E., Carapito C., Deshayes C., Reyrat J.-M., Van Dorsselaer A., Poch O., Schaeffer C., Lecompte O.
    Genome Res. 19:128-135(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 700084 / mc(2)155.
  4. "Radiation-sensitive gene A (RadA) targets DisA, DNA integrity scanning protein A, to negatively affect cyclic di-AMP synthesis activity in Mycobacterium smegmatis."
    Zhang L., He Z.G.
    J. Biol. Chem. 288:22426-22436(2013) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION AS AN ANTAGONIST OF DISA, OPERON STRUCTURE, INTERACTION WITH DISA.
    Strain: ATCC 700084 / mc(2)155.

Entry informationi

Entry nameiRADA_MYCS2
AccessioniPrimary (citable) accession number: A0R563
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 16, 2013
Last sequence update: January 9, 2007
Last modified: April 13, 2016
This is version 77 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.