A0R528 (KSTR_MYCS2) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
HTH-type transcriptional repressor KstR

Gene names

Name:	kstR
Ordered Locus Names:	MSMEG_6042, MSMEI_5881

Organism

Mycobacterium smegmatis (strain ATCC 700084 / mc(2)155) [Reference proteome] [HAMAP]

Taxonomic identifier

246196 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Actinobacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Corynebacterineae › Mycobacteriaceae › Mycobacterium › Mycobacterium smegmatis

Protein attributes

Sequence length	204 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Controls the expression of genes used for utilizing diverse lipids as energy sources. Ref.4
Subunit structure	Homodimer Probable.
Sequence similarities	Contains 1 HTH tetR-type DNA-binding domain.
Sequence caution	The sequence AFP42314.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.

Ontologies

Keywords
Biological process	Transcription Transcription regulation
Ligand	DNA-binding
Molecular function	Repressor
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	regulation of transcription, DNA-dependent Inferred from mutant phenotype Ref.4. Source: UniProtKB transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	DNA binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 204	204	HTH-type transcriptional repressor KstR		PRO_0000405029
Regions
	Domain	18 – 78	61	HTH tetR-type
	DNA binding	41 – 60	20	H-T-H motif By similarity

Sequences

	Sequence		Length	Mass (Da)
	A0R528 [UniParc]. Last modified January 9, 2007. Version 1. Checksum: 1125BEBE034F88C2	FASTA	204	22,571
	10 20 30 40 50 60 MTNVAVLSES ELGSEAQRER RKRILDATLA IASKGGYEAV QMRAVAERAD VAVGTLYRYF 70 80 90 100 110 120 PSKVHLLVSA LGREFERIDA KTDRAALAGG TPYQRLNFMV GKLNRAMQRN PLLTEAMTRA 130 140 150 160 170 180 FVFADASAAG EVDHVGKLMD SMFARAMSDG EPTEDQYHIA RVISDVWLSN LLAWLTRRAS 190 200 ATDVSKRLDL AVRLLIGTEE QPKI

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	Fleischmann R.D., Dodson R.J., Haft D.H., Merkel J.S., Nelson W.C., Fraser C.M. Submitted (OCT-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 700084 / mc(2)155.
[2]	"Interrupted coding sequences in Mycobacterium smegmatis: authentic mutations or sequencing errors?" Deshayes C., Perrodou E., Gallien S., Euphrasie D., Schaeffer C., Van-Dorsselaer A., Poch O., Lecompte O., Reyrat J.-M. Genome Biol. 8:R20.1-R20.9(2007) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 700084 / mc(2)155.
[3]	"Ortho-proteogenomics: multiple proteomes investigation through orthology and a new MS-based protocol." Gallien S., Perrodou E., Carapito C., Deshayes C., Reyrat J.-M., Van Dorsselaer A., Poch O., Schaeffer C., Lecompte O. Genome Res. 19:128-135(2009) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 700084 / mc(2)155.
[4]	"A highly conserved transcriptional repressor controls a large regulon involved in lipid degradation in Mycobacterium smegmatis and Mycobacterium tuberculosis." Kendall S.L., Withers M., Soffair C.N., Moreland N.J., Gurcha S., Sidders B., Frita R., Ten Bokum A., Besra G.S., Lott J.S., Stoker N.G. Mol. Microbiol. 65:684-699(2007) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION AS A TRANSCRIPTIONAL REPRESSOR.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000480 Genomic DNA. Translation: ABK75291.1. CP001663 Genomic DNA. Translation: AFP42314.1. Different initiation.
RefSeq	YP_006570609.1. NC_018289.1. YP_890266.1. NC_008596.1.
3D structure databases
ProteinModelPortal	A0R528.
ModBase	Search...
Protein-protein interaction databases
STRING	246196.MSMEG_6042.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	ABK75291; ABK75291; MSMEG_6042.
GeneID	13427211. 4531834.
KEGG	msg:MSMEI_5881. msm:MSMEG_6042.
PATRIC	18084317. VBIMycSme59918_5878.
Phylogenomic databases
eggNOG	NOG277692.
HOGENOM	HOG000052226.
OMA	TRAFMFA.
Enzyme and pathway databases
BioCyc	MSME246196:GJ4Y-6041-MONOMER.
Family and domain databases
Gene3D	1.10.357.10. 1 hit.
InterPro	IPR009057. Homeodomain-like. IPR001647. HTH_TetR. IPR015893. Tet_transcr_reg_TetR-like_C. [Graphical view]
Pfam	PF00440. TetR_N. 1 hit. [Graphical view]
PRINTS	PR00455. HTHTETR.
SUPFAM	SSF46689. Homeodomain_like. 1 hit.
PROSITE	PS01081. HTH_TETR_1. False negative. PS50977. HTH_TETR_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

KSTR_MYCS2

Accession

Primary (citable) accession number: A0R528
Secondary accession number(s): I7GG00

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 8, 2011
Last sequence update:	January 9, 2007
Last modified:	May 1, 2013
This is version 50 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program