A0R4S7 (ACDH2_MYCS2) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Acetaldehyde dehydrogenase 2
EC=1.2.1.10

Alternative name(s):
Acetaldehyde dehydrogenase [acetylating] 2

Gene names

Name:	mhpF
Ordered Locus Names:	MSMEG_5939, MSMEI_5779

Organism

Mycobacterium smegmatis (strain ATCC 700084 / mc(2)155) [Reference proteome] [HAMAP]

Taxonomic identifier

246196 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Actinobacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Corynebacterineae › Mycobacteriaceae › Mycobacterium › Mycobacterium smegmatis

Protein attributes

Sequence length	307 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the conversion of acetaldehyde to acetyl-CoA, using NAD+ and coenzyme A. Is the final enzyme in the meta-cleavage pathway for the degradation of aromatic compounds By similarity. HAMAP-Rule MF_01657
Catalytic activity	Acetaldehyde + CoA + NAD+ = acetyl-CoA + NADH. HAMAP-Rule MF_01657
Sequence similarities	Belongs to the acetaldehyde dehydrogenase family.
Sequence caution	The sequence ABK73662.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Biological process	Aromatic hydrocarbons catabolism
Ligand	NAD
Molecular function	Oxidoreductase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	aromatic compound catabolic process Inferred from electronic annotation. Source: HAMAP cellular amino acid metabolic process Inferred from electronic annotation. Source: InterPro
Cellular_component	cytoplasm Inferred from electronic annotation. Source: InterPro
Molecular_function	NAD binding Inferred from electronic annotation. Source: HAMAP acetaldehyde dehydrogenase (acetylating) activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 307	307	Acetaldehyde dehydrogenase 2 HAMAP-Rule MF_01657		PRO_0000387681
Regions
	Nucleotide binding	12 – 15	4	NAD By similarity
	Nucleotide binding	158 – 166	9	NAD By similarity
Sites
	Active site	127	1	Acyl-thioester intermediate By similarity
	Binding site	277	1	NAD By similarity

Sequences

	Sequence		Length	Mass (Da)
	A0R4S7 [UniParc]. Last modified November 3, 2009. Version 2. Checksum: A619D96D276AD6F5	FASTA	307	32,324
	10 20 30 40 50 60 MPKKSSVAIV GSGNISTDLL YKLLRSEWLE PRWMIGIDPE SEGLARARKL GLETSAEGVD 70 80 90 100 110 120 WLLAQSEKPD LVFEATSAYV HRDAAPRYEE AGIRAIDLTP AAVGPGVVPP ANLRDHLDAP 130 140 150 160 170 180 NVNMVTCGGQ ATIPIVHAVS RVVDVPYAEI VASVSSASAG PGTRANIDEF TKTTSAGVQN 190 200 210 220 230 240 IGGAQRGKAI IVLNPAEPPM IMRDTIFCAI PEGADHDAIT QSIKDVVAEV QTYVPGYRLL 250 260 270 280 290 300 NEPQFDEPSV VNGGNHLVTT FVEVEGAGDY LPPYAGNLDI MTAAATKVGE EIAKKSAEAS LASGAQA

References

[1]	Fleischmann R.D., Dodson R.J., Haft D.H., Merkel J.S., Nelson W.C., Fraser C.M. Submitted (OCT-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 700084 / mc(2)155.
[2]	"Interrupted coding sequences in Mycobacterium smegmatis: authentic mutations or sequencing errors?" Deshayes C., Perrodou E., Gallien S., Euphrasie D., Schaeffer C., Van-Dorsselaer A., Poch O., Lecompte O., Reyrat J.-M. Genome Biol. 8:R20.1-R20.9(2007) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 700084 / mc(2)155.
[3]	"Ortho-proteogenomics: multiple proteomes investigation through orthology and a new MS-based protocol." Gallien S., Perrodou E., Carapito C., Deshayes C., Reyrat J.-M., Van Dorsselaer A., Poch O., Schaeffer C., Lecompte O. Genome Res. 19:128-135(2009) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 700084 / mc(2)155.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000480 Genomic DNA. Translation: ABK73662.1. Different initiation. CP001663 Genomic DNA. Translation: AFP42212.1.
RefSeq	YP_006570507.1. NC_018289.1. YP_890165.1. NC_008596.1.
3D structure databases
ProteinModelPortal	A0R4S7.
ModBase	Search...
Protein-protein interaction databases
STRING	246196.MSMEG_5939.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	ABK73662; ABK73662; MSMEG_5939.
GeneID	13427109. 4531880.
KEGG	msg:MSMEI_5779. msm:MSMEG_5939.
PATRIC	18084117. VBIMycSme59918_5778.
Phylogenomic databases
eggNOG	COG4569.
HOGENOM	HOG000052149.
KO	K04073.
ProtClustDB	PRK08300.
Enzyme and pathway databases
BioCyc	MSME246196:GJ4Y-5938-MONOMER.
Family and domain databases
Gene3D	3.40.50.720. 1 hit.
HAMAP	MF_01657. Ac_ald_DH_ac.
InterPro	IPR003361. Acetaldehyde_dehydrogenase. IPR015426. Acetylaldehyde_DH_C. IPR016040. NAD(P)-bd_dom. IPR000534. Semialdehyde_DH_NAD-bd. [Graphical view]
PANTHER	PTHR21123. PTHR21123. 1 hit.
Pfam	PF09290. AcetDehyd-dimer. 1 hit. PF01118. Semialdhyde_dh. 1 hit. [Graphical view]
PIRSF	PIRSF015689. Actaldh_dh_actl. 1 hit.
SMART	SM00859. Semialdhyde_dh. 1 hit. [Graphical view]
TIGRFAMs	TIGR03215. ac_ald_DH_ac. 1 hit.
ProtoNet	Search...

Entry information

Entry name

ACDH2_MYCS2

Accession

Primary (citable) accession number: A0R4S7
Secondary accession number(s): I7G9C0

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 3, 2009
Last sequence update:	November 3, 2009
Last modified:	May 1, 2013
This is version 52 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program