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Protein

Steroid C26-monooxygenase

Gene

cyp142

Organism
Mycobacterium smegmatis (strain ATCC 700084 / mc(2)155)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Involved in the utilization of cholesterol as the sole carbon and energy source by degrading the side chain. Primarily catalyzes the sequential oxidation of the terminal methyl of cholest-4-en-3-one into (25R)-26-hydroxycholest-4-en-3-one (alcohol), (25R)-26-oxocholest-4-en-3-one (aldehyde), to finally yield the carboxylic acid (25R)-3-oxocholest-4-en-26-oate. Also able to sequentially oxidize cholesterol itself, not only cholest-4-en-3-one.2 Publications

Catalytic activityi

Cholest-4-en-3-one + 3 NADPH + 3 O2 = (25R)-3-oxocholest-4-en-26-oate + 3 NADP+ + 4 H2O.1 Publication1 Publication

Cofactori

heme2 Publications

Kineticsi

  1. KM=10.3 µM for cholest-4-en-3-one1 Publication
  2. KM=39.8 µM for cholesteryl sulfate1 Publication

    Pathwayi: cholesterol degradation

    This protein is involved in the pathway cholesterol degradation, which is part of Steroid metabolism.1 Publication
    View all proteins of this organism that are known to be involved in the pathway cholesterol degradation and in Steroid metabolism.

    Sites

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Metal bindingi343Iron (heme axial ligand)Combined sources2 Publications1

    GO - Molecular functioni

    GO - Biological processi

    Keywordsi

    Molecular functionMonooxygenase, Oxidoreductase
    Biological processCholesterol metabolism, Lipid degradation, Lipid metabolism, Steroid metabolism, Sterol metabolism
    LigandHeme, Iron, Metal-binding, NADP

    Enzyme and pathway databases

    UniPathwayiUPA01058.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Steroid C26-monooxygenase1 Publication (EC:1.14.13.2211 Publication1 Publication)
    Alternative name(s):
    Cholest-4-en-3-one C26-monooxygenase1 Publication
    Cholest-4-en-3-one C26-monooxygenase [(25R)-3-oxocholest-4-en-26-oate forming]1 Publication
    Cholesterol C26-monooxygenase1 Publication
    Cholesterol C26-monooxygenase [(25R)-3beta-hydroxycholest-5-en-26-oate forming]1 Publication
    Cytochrome P450 1421 Publication
    Steroid C27-monooxygenaseBy similarity
    Gene namesi
    Name:cyp1421 Publication
    Synonyms:cyp142A21 Publication
    Ordered Locus Names:MSMEG_5918Imported
    OrganismiMycobacterium smegmatis (strain ATCC 700084 / mc(2)155)
    Taxonomic identifieri246196 [NCBI]
    Taxonomic lineageiBacteriaActinobacteriaCorynebacterialesMycobacteriaceaeMycobacterium
    Proteomesi
    • UP000000757 Componenti: Chromosome

    Pathology & Biotechi

    Disruption phenotypei

    Cells lacking this gene show a slight reduction in the levels of cholest-4-en-3-one-26-oate, but the concentration of 26-hydroxycholest-4-en-3-one is not measurably affected. The levels of 26-hydroxycholest-4-en-3-one and cholest-4-on-3-one-26-oate are drastically reduced in cells lacking both cyp125A3 and cyp142A2.1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    ChainiPRO_00004387241 – 401Steroid C26-monooxygenaseAdd BLAST401

    Expressioni

    Inductioni

    By cholesterol.1 Publication

    Interactioni

    Protein-protein interaction databases

    STRINGi246196.MSMEG_5918.

    Structurei

    Secondary structure

    1401
    Legend: HelixTurnBeta strandPDB Structure known for this area
    Show more details
    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Helixi15 – 19Combined sources5
    Helixi23 – 33Combined sources11
    Beta strandi35 – 38Combined sources4
    Beta strandi44 – 46Combined sources3
    Helixi49 – 57Combined sources9
    Turni59 – 61Combined sources3
    Beta strandi62 – 64Combined sources3
    Beta strandi70 – 72Combined sources3
    Helixi78 – 80Combined sources3
    Helixi85 – 93Combined sources9
    Helixi94 – 96Combined sources3
    Helixi99 – 103Combined sources5
    Helixi106 – 121Combined sources16
    Beta strandi124 – 127Combined sources4
    Helixi128 – 131Combined sources4
    Turni132 – 134Combined sources3
    Helixi135 – 145Combined sources11
    Helixi149 – 151Combined sources3
    Helixi152 – 163Combined sources12
    Beta strandi168 – 170Combined sources3
    Helixi172 – 198Combined sources27
    Helixi204 – 209Combined sources6
    Helixi220 – 235Combined sources16
    Helixi238 – 251Combined sources14
    Helixi253 – 261Combined sources9
    Helixi263 – 265Combined sources3
    Helixi266 – 277Combined sources12
    Beta strandi282 – 289Combined sources8
    Beta strandi291 – 293Combined sources3
    Beta strandi296 – 298Combined sources3
    Beta strandi303 – 307Combined sources5
    Helixi308 – 311Combined sources4
    Helixi315 – 318Combined sources4
    Helixi339 – 341Combined sources3
    Helixi346 – 363Combined sources18
    Beta strandi368 – 371Combined sources4
    Beta strandi381 – 383Combined sources3
    Beta strandi390 – 392Combined sources3

    3D structure databases

    Select the link destinations:
    PDBei
    RCSB PDBi
    PDBji
    Links Updated
    PDB entryMethodResolution (Å)ChainPositionsPDBsum
    2YOOX-ray1.69A/B/C/D4-401[»]
    3ZBYX-ray1.93A/B/C/D/E/F1-401[»]
    4TRIX-ray2.00A/B1-401[»]
    4UAXX-ray1.78A1-401[»]
    ProteinModelPortaliA0R4Q6.
    SMRiA0R4Q6.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the cytochrome P450 family.Curated

    Phylogenomic databases

    eggNOGiENOG410719E. Bacteria.
    ENOG410YPZF. LUCA.
    HOGENOMiHOG000243680.
    KOiK16046.
    OMAiAYAWMRA.
    OrthoDBiPOG091H0API.

    Family and domain databases

    Gene3Di1.10.630.10. 1 hit.
    InterProiView protein in InterPro
    IPR001128. Cyt_P450.
    IPR002397. Cyt_P450_B.
    IPR017972. Cyt_P450_CS.
    PfamiView protein in Pfam
    PF00067. p450. 1 hit.
    PRINTSiPR00359. BP450.
    PR00385. P450.
    SUPFAMiSSF48264. SSF48264. 1 hit.
    PROSITEiView protein in PROSITE
    PS00086. CYTOCHROME_P450. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    A0R4Q6-1 [UniParc]FASTAAdd to basket

    « Hide

            10         20         30         40         50
    MTQMLTRPDV DLVNGMFYAD GGAREAYRWM RANEPVFRDR NGLAAATTYQ
    60 70 80 90 100
    AVLDAERNPE LFSSTGGIRP DQPGMPYMID MDDPQHLLRR KLVNAGFTRK
    110 120 130 140 150
    RVMDKVDSIG RLCDTLIDAV CERGECDFVR DIAAPLPMAV IGDMLGVLPT
    160 170 180 190 200
    ERDMLLKWSD DLVCGLSSHV DEAAIQKLMD TFAAYTEFTK DVITKRRAEP
    210 220 230 240 250
    TDDLFSVLVN SEVEGQRMSD DEIVFETLLI LIGGDETTRH TLSGGTEQLL
    260 270 280 290 300
    RHRDQWDALV ADVDLLPGAI EEMLRWTSPV KNMCRTLTAD TVFHGTELRA
    310 320 330 340 350
    GEKIMLMFES ANFDESVFGD PDNFRIDRNP NSHVAFGFGT HFCLGNQLAR
    360 370 380 390 400
    LELRLMTERV LRRLPDLRLA DDAPVPLRPA NFVSGPESMP VVFTPSAPVL

    A
    Length:401
    Mass (Da):44,901
    Last modified:January 9, 2007 - v1
    Checksum:i9195ECA2F9D8D509
    GO

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    CP000480 Genomic DNA. Translation: ABK74975.1.
    RefSeqiWP_011730892.1. NZ_CP009494.1.
    YP_890144.1. NC_008596.1.

    Genome annotation databases

    EnsemblBacteriaiABK74975; ABK74975; MSMEG_5918.
    GeneIDi4533135.
    KEGGimsb:LJ00_29265.
    msm:MSMEG_5918.
    PATRICi18084077. VBIMycSme59918_5758.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    CP000480 Genomic DNA. Translation: ABK74975.1.
    RefSeqiWP_011730892.1. NZ_CP009494.1.
    YP_890144.1. NC_008596.1.

    3D structure databases

    Select the link destinations:
    PDBei
    RCSB PDBi
    PDBji
    Links Updated
    PDB entryMethodResolution (Å)ChainPositionsPDBsum
    2YOOX-ray1.69A/B/C/D4-401[»]
    3ZBYX-ray1.93A/B/C/D/E/F1-401[»]
    4TRIX-ray2.00A/B1-401[»]
    4UAXX-ray1.78A1-401[»]
    ProteinModelPortaliA0R4Q6.
    SMRiA0R4Q6.
    ModBaseiSearch...
    MobiDBiSearch...

    Protein-protein interaction databases

    STRINGi246196.MSMEG_5918.

    Protocols and materials databases

    Structural Biology KnowledgebaseSearch...

    Genome annotation databases

    EnsemblBacteriaiABK74975; ABK74975; MSMEG_5918.
    GeneIDi4533135.
    KEGGimsb:LJ00_29265.
    msm:MSMEG_5918.
    PATRICi18084077. VBIMycSme59918_5758.

    Phylogenomic databases

    eggNOGiENOG410719E. Bacteria.
    ENOG410YPZF. LUCA.
    HOGENOMiHOG000243680.
    KOiK16046.
    OMAiAYAWMRA.
    OrthoDBiPOG091H0API.

    Enzyme and pathway databases

    UniPathwayiUPA01058.

    Family and domain databases

    Gene3Di1.10.630.10. 1 hit.
    InterProiView protein in InterPro
    IPR001128. Cyt_P450.
    IPR002397. Cyt_P450_B.
    IPR017972. Cyt_P450_CS.
    PfamiView protein in Pfam
    PF00067. p450. 1 hit.
    PRINTSiPR00359. BP450.
    PR00385. P450.
    SUPFAMiSSF48264. SSF48264. 1 hit.
    PROSITEiView protein in PROSITE
    PS00086. CYTOCHROME_P450. 1 hit.
    ProtoNetiSearch...

    Entry informationi

    Entry nameiCP142_MYCS2
    AccessioniPrimary (citable) accession number: A0R4Q6
    Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 18, 2017
    Last sequence update: January 9, 2007
    Last modified: April 12, 2017
    This is version 83 of the entry and version 1 of the sequence. See complete history.
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    3. SIMILARITY comments
      Index of protein domains and families

    Similar proteinsi

    Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
    100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
    90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
    50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.