A0R4M9 (OTSA_MYCS2) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Trehalose-phosphate synthase
Short name=TPS
EC=2.4.1.-

Alternative name(s):
Trehalose-6-phosphate synthase

Gene names

Name:	otsA
Ordered Locus Names:	MSMEG_5892

Organism

Mycobacterium smegmatis (strain ATCC 700084 / mc(2)155)

Taxonomic identifier

246196 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Corynebacterineae › Mycobacteriaceae › Mycobacterium

Protein attributes

Sequence length	503 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Catalyzes the transfer of glucose from a nucleoside diphosphate-glucose to glucose-6-phosphate to form trehalose-6-phosphate and nucleoside diphosphate By similarity.
Catalytic activity	Nucleoside diphosphate-glucose + D-glucose 6-phosphate = trehalose 6-phosphate + nucleoside diphosphate.
Pathway	Glycan biosynthesis; trehalose biosynthesis.
Subunit structure	Homotetramer By similarity.
Sequence similarities	Belongs to the glycosyltransferase 20 family.

Ontologies

Keywords
Molecular function	Glycosyltransferase Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	trehalose biosynthetic process Inferred from electronic annotation. Source: InterPro
Molecular function	transferase activity, transferring glycosyl groups Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 503	503	Trehalose-phosphate synthase		PRO_0000348910
Regions
	Region	42 – 43	2	NDP-glucose binding By similarity
	Region	393 – 397	5	NDP-glucose binding By similarity
Sites
	Binding site	22	1	Glucose-6-phosphate By similarity
	Binding site	94	1	Glucose-6-phosphate By similarity
	Binding site	148	1	Glucose-6-phosphate By similarity
	Binding site	290	1	NDP-glucose By similarity
	Binding site	295	1	NDP-glucose By similarity
	Binding site	328	1	Glucose-6-phosphate By similarity
	Site	103	1	Involved in alpha anomer selectivity By similarity
	Site	173	1	Involved in alpha anomer selectivity By similarity

Sequences

	Sequence		Length	Mass (Da)
	A0R4M9 [UniParc]. Last modified January 9, 2007. Version 1. Checksum: 3D4B5CE2E6DA5ACF	FASTA	503	56,280
	10 20 30 40 50 60 MSPESGHETI SGTSDFVVVA NRLPVDLERL PDGTTRWKRS PGGLVTALEP LLRKRRGSWI 70 80 90 100 110 120 GWAGVADSDE EPIVQDGLQL HPVRLSADDV AKYYEGFSNA TLWPLYHDLI VKPEYHREWW 130 140 150 160 170 180 DRYVEVNRRF AEATARAAAE GATVWIQDYQ LQLVPKMLRM LRPDVTIGFF LHIPFPPVEL 190 200 210 220 230 240 FMQMPWRTEI VEGLLGADLV GFHLPGGAQN FLVLSRRLVG ANTSRASIGV RSRFGEVQVG 250 260 270 280 290 300 FRTVKVGAFP ISIDSAELDG KARNRAIRQR ARQIRAELGN PRKIMLGVDR LDYTKGIDVR 310 320 330 340 350 360 LRALSELLEE KRIKRDDTVL VQLATPSRER VESYIAMRED IERQVGHING EYGEVGHPIV 370 380 390 400 410 420 HYLHRPIPRD ELIAFFVAAD VMLVTPLRDG MNLVAKEYVA CRSDLGGALV LSEFTGAAAE 430 440 450 460 470 480 LRQAYLVNPH DLEGVKDKIE AAVNQNPEEG KRRMRALRRQ VLAHDVDRWA RSFLDALAAT 490 500 GETGDSGVTG ESTPAPESDS GSF

References

[1]	Fleischmann R.D., Dodson R.J., Haft D.H., Merkel J.S., Nelson W.C., Fraser C.M. Submitted (OCT-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 700084 / mc(2)155.
[2]	"Ortho-proteogenomics: multiple proteomes investigation through orthology and a new MS-based protocol." Gallien S., Perrodou E., Carapito C., Deshayes C., Reyrat J.-M., Van Dorsselaer A., Poch O., Schaeffer C., Lecompte O. Genome Res. 19:128-135(2009) [PubMed: 18955433] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000480 Genomic DNA. Translation: ABK71484.1.
RefSeq	YP_890117.1. NC_008596.1.
3D structure databases
ProteinModelPortal	A0R4M9.
ModBase	Search...
Protein-protein interaction databases
STRING	A0R4M9.
Protein family/group databases
CAZy	GT20. Glycosyltransferase Family 20.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBMYCT00000042723; EBMYCP00000041060; EBMYCG00000042718.
GeneID	4532640.
GenomeReviews	Gene locus MSMEG_5892 in contig CP000480_GR.
KEGG	msm:MSMEG_5892.
NMPDR	fig|246196.1.peg.5854.
PATRIC	18084027. VBIMycSme59918_5733.
TIGR	MSMEG_5892.
Phylogenomic databases
eggNOG	COG0380.
GeneTree	EBGT00050000016525.
HOGENOM	HBG559076.
OMA	EYGRKEM.
PhylomeDB	A0R4M9.
ProtClustDB	CLSK872185.
Enzyme and pathway databases
BioCyc	MSME246196:MSMEG_5892-MONOMER.
Family and domain databases
InterPro	IPR001830. Glyco_trans_20. [Graphical view]
KO	K00697.
Pfam	PF00982. Glyco_transf_20. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

OTSA_MYCS2

Accession

Primary (citable) accession number: A0R4M9

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 2, 2008
Last sequence update:	January 9, 2007
Last modified:	December 14, 2011
This is version 36 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program