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Protein

Phosphoribosylaminoimidazole-succinocarboxamide synthase

Gene

purC

Organism
Mycobacterium smegmatis (strain ATCC 700084 / mc(2)155)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

ATP + 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxylate + L-aspartate = ADP + phosphate + (S)-2-(5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamido)succinate.UniRule annotation

Pathwayi: IMP biosynthesis via de novo pathway

This protein is involved in step 1 of the subpathway that synthesizes 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide from 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxylate.UniRule annotation
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. Phosphoribosylaminoimidazole-succinocarboxamide synthase (purC)
  2. Adenylosuccinate lyase (purB), Adenylosuccinate lyase (purB)
This subpathway is part of the pathway IMP biosynthesis via de novo pathway, which is itself part of Purine metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide from 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxylate, the pathway IMP biosynthesis via de novo pathway and in Purine metabolism.

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Biological processi

Purine biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciMSME246196:GJ4Y-5840-MONOMER.
UniPathwayiUPA00074; UER00131.

Names & Taxonomyi

Protein namesi
Recommended name:
Phosphoribosylaminoimidazole-succinocarboxamide synthaseUniRule annotation (EC:6.3.2.6UniRule annotation)
Alternative name(s):
SAICAR synthetaseUniRule annotation
Gene namesi
Name:purCUniRule annotation
Ordered Locus Names:MSMEG_5841, MSMEI_5683
OrganismiMycobacterium smegmatis (strain ATCC 700084 / mc(2)155)
Taxonomic identifieri246196 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaCorynebacterialesMycobacteriaceaeMycobacterium
Proteomesi
  • UP000006158 Componenti: Chromosome
  • UP000000757 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 299299Phosphoribosylaminoimidazole-succinocarboxamide synthasePRO_1000018735Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi246196.MSMEG_5841.

Structurei

3D structure databases

ProteinModelPortaliA0R4I0.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the SAICAR synthetase family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105C8V. Bacteria.
COG0152. LUCA.
HOGENOMiHOG000230360.
KOiK01923.
OMAiHFPDPDI.
OrthoDBiEOG69SKD1.

Family and domain databases

Gene3Di3.30.470.20. 1 hit.
HAMAPiMF_00137. SAICAR_synth.
InterProiIPR013816. ATP_grasp_subdomain_2.
IPR028923. SAICAR_synt/ADE2_N.
IPR001636. SAICAR_synth.
IPR018236. SAICAR_synthetase_CS.
[Graphical view]
PfamiPF01259. SAICAR_synt. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00081. purC. 1 hit.
PROSITEiPS01057. SAICAR_SYNTHETASE_1. 1 hit.
PS01058. SAICAR_SYNTHETASE_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

A0R4I0-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRPALSDYQH LASGKVREIY RIDDEHLLFV ASDRISAYDY ILDSQIPDKG
60 70 80 90 100
RILTAMSVFF FDHLLRTAGV PNHLAGPPDD ERIPADVLGR ALVVRRLDML
110 120 130 140 150
PVECVARGYL TGSGLIDYEK TGTVCGIALP PGLGEASKFD EPLFTPATKA
160 170 180 190 200
EIGEHDENIS FAKVIELVGA ELANQLRDRT LQTYTAGADH ALSKGIIIAD
210 220 230 240 250
TKFEFGVDRD GTVVLADEVF TPDSSRYWRA DSYQPGVVQN SFDKQFVRNW
260 270 280 290
LTGPESGWDR HGNTPPPALP DDIVAATRER YIEAYERISG LSFDDWIGA
Length:299
Mass (Da):33,101
Last modified:January 9, 2007 - v1
Checksum:i768DD4C7E5255BB0
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000480 Genomic DNA. Translation: ABK73534.1.
CP001663 Genomic DNA. Translation: AFP42118.1.
RefSeqiWP_003897243.1. NZ_CP009494.1.
YP_890068.1. NC_008596.1.

Genome annotation databases

EnsemblBacteriaiABK73534; ABK73534; MSMEG_5841.
AFP42118; AFP42118; MSMEI_5683.
GeneIDi4533235.
KEGGimsb:LJ00_28885.
msg:MSMEI_5683.
msm:MSMEG_5841.
PATRICi18083929. VBIMycSme59918_5685.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000480 Genomic DNA. Translation: ABK73534.1.
CP001663 Genomic DNA. Translation: AFP42118.1.
RefSeqiWP_003897243.1. NZ_CP009494.1.
YP_890068.1. NC_008596.1.

3D structure databases

ProteinModelPortaliA0R4I0.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi246196.MSMEG_5841.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABK73534; ABK73534; MSMEG_5841.
AFP42118; AFP42118; MSMEI_5683.
GeneIDi4533235.
KEGGimsb:LJ00_28885.
msg:MSMEI_5683.
msm:MSMEG_5841.
PATRICi18083929. VBIMycSme59918_5685.

Phylogenomic databases

eggNOGiENOG4105C8V. Bacteria.
COG0152. LUCA.
HOGENOMiHOG000230360.
KOiK01923.
OMAiHFPDPDI.
OrthoDBiEOG69SKD1.

Enzyme and pathway databases

UniPathwayiUPA00074; UER00131.
BioCyciMSME246196:GJ4Y-5840-MONOMER.

Family and domain databases

Gene3Di3.30.470.20. 1 hit.
HAMAPiMF_00137. SAICAR_synth.
InterProiIPR013816. ATP_grasp_subdomain_2.
IPR028923. SAICAR_synt/ADE2_N.
IPR001636. SAICAR_synth.
IPR018236. SAICAR_synthetase_CS.
[Graphical view]
PfamiPF01259. SAICAR_synt. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00081. purC. 1 hit.
PROSITEiPS01057. SAICAR_SYNTHETASE_1. 1 hit.
PS01058. SAICAR_SYNTHETASE_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Fleischmann R.D., Dodson R.J., Haft D.H., Merkel J.S., Nelson W.C., Fraser C.M.
    Submitted (OCT-2006) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 700084 / mc(2)155.
  2. "Interrupted coding sequences in Mycobacterium smegmatis: authentic mutations or sequencing errors?"
    Deshayes C., Perrodou E., Gallien S., Euphrasie D., Schaeffer C., Van-Dorsselaer A., Poch O., Lecompte O., Reyrat J.-M.
    Genome Biol. 8:R20.1-R20.9(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 700084 / mc(2)155.
  3. "Ortho-proteogenomics: multiple proteomes investigation through orthology and a new MS-based protocol."
    Gallien S., Perrodou E., Carapito C., Deshayes C., Reyrat J.-M., Van Dorsselaer A., Poch O., Schaeffer C., Lecompte O.
    Genome Res. 19:128-135(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS], IDENTIFICATION OF N-TERMINUS.
    Strain: ATCC 700084 / mc(2)155.

Entry informationi

Entry nameiPUR7_MYCS2
AccessioniPrimary (citable) accession number: A0R4I0
Secondary accession number(s): I7FTB0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: January 9, 2007
Last modified: November 11, 2015
This is version 63 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.