A0R4I0 (PUR7_MYCS2) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Phosphoribosylaminoimidazole-succinocarboxamide synthase
EC=6.3.2.6

Alternative name(s):
SAICAR synthetase

Gene names

Name:	purC
Ordered Locus Names:	MSMEG_5841

Organism

Mycobacterium smegmatis (strain ATCC 700084 / mc(2)155)

Taxonomic identifier

246196 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Corynebacterineae › Mycobacteriaceae › Mycobacterium

Protein attributes

Sequence length	299 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Catalytic activity	ATP + 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxylate + L-aspartate = ADP + phosphate + (S)-2-(5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamido)succinate. HAMAP MF_00137
Pathway	Purine metabolism; IMP biosynthesis via de novo pathway; 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide from 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxylate: step 1/2. HAMAP MF_00137
Sequence similarities	Belongs to the SAICAR synthetase family.

Ontologies

Keywords
Biological process	Purine biosynthesis
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	purine nucleotide biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW phosphoribosylaminoimidazolesuccinocarboxamide synthase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 299	299	Phosphoribosylaminoimidazole-succinocarboxamide synthase HAMAP MF_00137		PRO_1000018735

Sequences

	Sequence		Length	Mass (Da)
	A0R4I0 [UniParc]. Last modified January 9, 2007. Version 1. Checksum: 768DD4C7E5255BB0	FASTA	299	33,101
	10 20 30 40 50 60 MRPALSDYQH LASGKVREIY RIDDEHLLFV ASDRISAYDY ILDSQIPDKG RILTAMSVFF 70 80 90 100 110 120 FDHLLRTAGV PNHLAGPPDD ERIPADVLGR ALVVRRLDML PVECVARGYL TGSGLIDYEK 130 140 150 160 170 180 TGTVCGIALP PGLGEASKFD EPLFTPATKA EIGEHDENIS FAKVIELVGA ELANQLRDRT 190 200 210 220 230 240 LQTYTAGADH ALSKGIIIAD TKFEFGVDRD GTVVLADEVF TPDSSRYWRA DSYQPGVVQN 250 260 270 280 290 SFDKQFVRNW LTGPESGWDR HGNTPPPALP DDIVAATRER YIEAYERISG LSFDDWIGA

References

[1]	Fleischmann R.D., Dodson R.J., Haft D.H., Merkel J.S., Nelson W.C., Fraser C.M. Submitted (OCT-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 700084 / mc(2)155.
[2]	"Ortho-proteogenomics: multiple proteomes investigation through orthology and a new MS-based protocol." Gallien S., Perrodou E., Carapito C., Deshayes C., Reyrat J.-M., Van Dorsselaer A., Poch O., Schaeffer C., Lecompte O. Genome Res. 19:128-135(2009) [PubMed: 18955433] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS], IDENTIFICATION OF N-TERMINUS.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000480 Genomic DNA. Translation: ABK73534.1.
RefSeq	YP_890068.1. NC_008596.1.
3D structure databases
ProteinModelPortal	A0R4I0.
ModBase	Search...
Protein-protein interaction databases
STRING	A0R4I0.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBMYCT00000043268; EBMYCP00000041605; EBMYCG00000043263.
GeneID	4533235.
GenomeReviews	Gene locus MSMEG_5841 in contig CP000480_GR.
KEGG	msm:MSMEG_5841.
NMPDR	fig|246196.1.peg.5807.
PATRIC	18083929. VBIMycSme59918_5685.
TIGR	MSMEG_5841.
Phylogenomic databases
eggNOG	COG0152.
GeneTree	EBGT00050000017539.
HOGENOM	HBG555040.
OMA	SSRIWDG.
PhylomeDB	A0R4I0.
ProtClustDB	PRK13961.
Enzyme and pathway databases
BioCyc	MSME246196:MSMEG_5841-MONOMER.
Family and domain databases
HAMAP	MF_00137. SAICAR_synth. [Tree]
InterPro	IPR013816. ATP_grasp_subdomain_2. IPR001636. SAICAR_synth. IPR018236. SAICAR_synthetase_CS. [Graphical view]
Gene3D	G3DSA:3.30.470.20. ATP_grasp_subdomain_2. 1 hit.
KO	K01923.
PANTHER	PTHR11609. SAICAR_synt. 1 hit.
Pfam	PF01259. SAICAR_synt. 1 hit. [Graphical view]
TIGRFAMs	TIGR00081. PurC. 1 hit.
PROSITE	PS01057. SAICAR_SYNTHETASE_1. 1 hit. PS01058. SAICAR_SYNTHETASE_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PUR7_MYCS2

Accession

Primary (citable) accession number: A0R4I0

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	January 9, 2007
Last modified:	December 14, 2011
This is version 36 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program