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A0R4E0 (A0R4E0_MYCS2) Unreviewed, UniProtKB/TrEMBL

Last modified January 25, 2012. Version 40. Feed History...

Clusters with 100%, 90%, 50% identity | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Amidophosphoribosyltransferase PIRNR PIRNR000485
Short name=ATase PIRNR PIRNR000485
EC=2.4.2.14 PIRNR PIRNR000485
Alternative name(s):
Glutamine phosphoribosylpyrophosphate amidotransferase PIRNR PIRNR000485
Gene names
Name:purF EMBL ABK71241.1
Ordered Locus Names:MSMEG_5800
OrganismMycobacterium smegmatis (strain ATCC 700084 / mc(2)155) EMBL ABK71241.1
Taxonomic identifier246196 [NCBI]
Taxonomic lineageBacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Corynebacterineae › Mycobacteriaceae › Mycobacterium

Protein attributes

Sequence length511 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

5-phospho-beta-D-ribosylamine + diphosphate + L-glutamate = L-glutamine + 5-phospho-alpha-D-ribose 1-diphosphate + H2O. PIRNR PIRNR000485

Cofactor

Binds 1 4Fe-4S cluster per subunit By similarity. PIRSR PIRSR000485-3

Binds 1 magnesium ion per subunit By similarity. PIRNR PIRNR000485

Pathway

Purine metabolism; IMP biosynthesis via de novo pathway; N(1)-(5-phospho-D-ribosyl)glycinamide from 5-phospho-alpha-D-ribose 1-diphosphate: step 1/2. PIRNR PIRNR000485

Sequence similarities

In the C-terminal section; belongs to the purine/pyrimidine phosphoribosyltransferase family. PIRNR PIRNR000485

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Sites

Active site171For GATase activity By similarity PIRSR PIRSR000485-1
Metal binding2581Iron-sulfur (4Fe-4S) By similarity PIRSR PIRSR000485-3
Metal binding4041Iron-sulfur (4Fe-4S) By similarity PIRSR PIRSR000485-3
Metal binding4621Iron-sulfur (4Fe-4S) By similarity PIRSR PIRSR000485-3
Metal binding4651Iron-sulfur (4Fe-4S) By similarity PIRSR PIRSR000485-3

Sequences

Sequence LengthMass (Da)Tools
A0R4E0 [UniParc].

Last modified January 9, 2007. Version 1.
Checksum: 07D7AF16B38AAA6B

FASTA51154,536
        10         20         30         40         50         60 
MIGHDDNESE NEPREECGVF GVWAPGEEVA KLTYYGLYAL QHRGQEAAGI AVADGSQVLV 

        70         80         90        100        110        120 
FKDLGLVSQV FDEQTLAAMP GHVAVGHCRY STTGSTTWEN AQPVFRNTAA GTGVALGHNG 

       130        140        150        160        170        180 
NLVNTAELAA RARDNGLIEL KGGPAATTDS DILGALLAHG AADATLEQAA LELLPTVRGA 

       190        200        210        220        230        240 
FCLTFMDENT LYAARDPHGV RPLSLGRLDR GWVVASETAA LDIVGASFVR DIEPGELLAI 

       250        260        270        280        290        300 
DADGVRSTRF ANPEPKGCVF EYVYLARPDS TLVGRSVHAT RVDIGRRLAR EHPVEADLVI 

       310        320        330        340        350        360 
GVPESGTPAA VGYAQESGIP FGQGLMKNAY VGRTFIQPSQ TIRQLGIRLK LNPLREVIRG 

       370        380        390        400        410        420 
KRLIVVDDSI VRGNTQRALV RMLREAGALE VHVRIASPPV RWPCFYGIDF ATPAELIANA 

       430        440        450        460        470        480 
ASQEHEGEML EAVRHAIGAD SLGYISQQGM IAATEQPASR LCSACFDGKY PIELPGETAL 

       490        500        510 
GKNVIEHMLA SAARSGIPVA QNDNASALRR P

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References

[1]Fleischmann R.D., Dodson R.J., Haft D.H., Merkel J.S., Nelson W.C., Fraser C.M.
Submitted (OCT-2006) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 700084 / mc(2)155.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		CP000480 Genomic DNA. Translation: ABK71241.1.
RefSeqYP_890028.1. NC_008596.1.

3D structure databases

ProteinModelPortalA0R4E0.
ModBaseSearch...

Protein-protein interaction databases

STRINGA0R4E0.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaEBMYCT00000044720; EBMYCP00000043057; EBMYCG00000044715.
GeneID4537244.
GenomeReviewsGene locus MSMEG_5800 in contig CP000480_GR.
KEGGmsm:MSMEG_5800.
NMPDRfig|246196.1.peg.5768.
PATRIC18083847. VBIMycSme59918_5644.
TIGRMSMEG_5800.

Phylogenomic databases

eggNOGCOG0034.
GeneTreeEBGT00050000016850.
HOGENOMHBG392416.
OMAVWAPGEE.
ProtClustDBPRK07847.

Family and domain databases

InterProIPR005854. Amd_phspho_trans.
IPR000583. GATase_2.
IPR017932. GATase_II.
IPR000836. PRibTrfase.
[Graphical view]
KOK00764.
PANTHERPTHR11907. Amd_phspho_trans. 1 hit.
PfamPF00310. GATase_2. 1 hit.
PF00156. Pribosyltran. 1 hit.
[Graphical view]
PIRSFPIRSF000485. Amd_phspho_trans. 1 hit.
TIGRFAMsTIGR01134. PurF. 1 hit.
PROSITEPS51278. GATASE_TYPE_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameA0R4E0_MYCS2
AccessionPrimary (citable) accession number: A0R4E0
Entry history
Integrated into UniProtKB/TrEMBL: January 9, 2007
Last sequence update: January 9, 2007
Last modified: January 25, 2012
This is version 40 of the entry and version 1 of the sequence. [Complete history]
Entry statusUnreviewed (UniProtKB/TrEMBL)
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