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Protein

Phosphate import ATP-binding protein PstB

Gene

pstB

Organism
Mycobacterium smegmatis (strain ATCC 700084 / mc(2)155)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Part of the ABC transporter complex PstSACB involved in phosphate import. Responsible for energy coupling to the transport system.UniRule annotation

Catalytic activityi

ATP + H2O + phosphate(Out) = ADP + phosphate + phosphate(In).UniRule annotation

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi37 – 44ATPUniRule annotation8

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

Phosphate transport, Transport

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Phosphate import ATP-binding protein PstBUniRule annotation (EC:3.6.3.27UniRule annotation)
Alternative name(s):
ABC phosphate transporterUniRule annotation
Phosphate-transporting ATPaseUniRule annotation
Gene namesi
Name:pstBUniRule annotation
Synonyms:mtp1, pstB1
Ordered Locus Names:MSMEG_5779, MSMEI_5626
OrganismiMycobacterium smegmatis (strain ATCC 700084 / mc(2)155)
Taxonomic identifieri246196 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaCorynebacterialesMycobacteriaceaeMycobacterium
Proteomesi
  • UP000006158 Componenti: Chromosome
  • UP000000757 Componenti: Chromosome

Subcellular locationi

  • Cell membrane UniRule annotation; Peripheral membrane protein UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002936001 – 258Phosphate import ATP-binding protein PstBAdd BLAST258

Interactioni

Subunit structurei

The complex is composed of two ATP-binding proteins (PstB), two transmembrane proteins (PstC and PstA) and a solute-binding protein (PstS).UniRule annotation

Protein-protein interaction databases

STRINGi246196.MSMEG_5779.

Structurei

3D structure databases

ProteinModelPortaliA0R4C0.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini5 – 247ABC transporterUniRule annotationAdd BLAST243

Sequence similaritiesi

Belongs to the ABC transporter superfamily. Phosphate importer (TC 3.A.1.7) family. [View classification]UniRule annotation
Contains 1 ABC transporter domain.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105BZY. Bacteria.
COG1117. LUCA.
KOiK02036.
OMAiMTVEPRS.
OrthoDBiPOG091H01HF.

Family and domain databases

CDDicd03260. ABC_PstB_phosphate_transporter. 1 hit.
Gene3Di3.40.50.300. 1 hit.
InterProiIPR003593. AAA+_ATPase.
IPR003439. ABC_transporter-like.
IPR017871. ABC_transporter_CS.
IPR015850. ABC_transpr_PstB.
IPR027417. P-loop_NTPase.
IPR005670. Phosp_transpt1.
[Graphical view]
PfamiPF00005. ABC_tran. 1 hit.
[Graphical view]
SMARTiSM00382. AAA. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR00972. 3a0107s01c2. 1 hit.
PROSITEiPS00211. ABC_TRANSPORTER_1. 1 hit.
PS50893. ABC_TRANSPORTER_2. 1 hit.
PS51238. PSTB. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

A0R4C0-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAKRLDLKDV NIYYGAFHAV ADVSLAVQPR SVTAFIGPSG CGKSTVLRTL
60 70 80 90 100
NRMHEVIPGA RVEGSVLLDG EDIYGPGVDP VGVRKTIGMV FQRPNPFPTM
110 120 130 140 150
SIRDNVVAGL KLQGVRNKKT LDEVAERSLR GANLWNEVKD RLDKPGGGLS
160 170 180 190 200
GGQQQRLCIA RAIAVQPDVL LMDEPCSALD PISTLAIEDL IATLKLDYTI
210 220 230 240 250
VIVTHNMQQA ARVSDQTAFF NLEATGKPGR LIEIDDTEKI FSNPRQKATE

DYISGRFG
Length:258
Mass (Da):28,101
Last modified:January 9, 2007 - v1
Checksum:i2613D1CB231C3B9E
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti9D → E in AAC15686 (PubMed:9541026).Curated1
Sequence conflicti16 – 38AFHAV…AFIGP → GSTRLPTCHSRCSRAASRRI ISQ in AAC15686 (PubMed:9541026).CuratedAdd BLAST23
Sequence conflicti106V → A in AAC15686 (PubMed:9541026).Curated1
Sequence conflicti174E → D in AAC15686 (PubMed:9541026).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF045938 Genomic DNA. Translation: AAC15686.1.
CP000480 Genomic DNA. Translation: ABK72426.1.
CP001663 Genomic DNA. Translation: AFP42061.1.
RefSeqiWP_011730783.1. NZ_CP009494.1.
YP_890008.1. NC_008596.1.

Genome annotation databases

EnsemblBacteriaiABK72426; ABK72426; MSMEG_5779.
AFP42061; AFP42061; MSMEI_5626.
GeneIDi4536762.
KEGGimsb:LJ00_28575.
msg:MSMEI_5626.
msm:MSMEG_5779.
PATRICi18083809. VBIMycSme59918_5625.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF045938 Genomic DNA. Translation: AAC15686.1.
CP000480 Genomic DNA. Translation: ABK72426.1.
CP001663 Genomic DNA. Translation: AFP42061.1.
RefSeqiWP_011730783.1. NZ_CP009494.1.
YP_890008.1. NC_008596.1.

3D structure databases

ProteinModelPortaliA0R4C0.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi246196.MSMEG_5779.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABK72426; ABK72426; MSMEG_5779.
AFP42061; AFP42061; MSMEI_5626.
GeneIDi4536762.
KEGGimsb:LJ00_28575.
msg:MSMEI_5626.
msm:MSMEG_5779.
PATRICi18083809. VBIMycSme59918_5625.

Phylogenomic databases

eggNOGiENOG4105BZY. Bacteria.
COG1117. LUCA.
KOiK02036.
OMAiMTVEPRS.
OrthoDBiPOG091H01HF.

Family and domain databases

CDDicd03260. ABC_PstB_phosphate_transporter. 1 hit.
Gene3Di3.40.50.300. 1 hit.
InterProiIPR003593. AAA+_ATPase.
IPR003439. ABC_transporter-like.
IPR017871. ABC_transporter_CS.
IPR015850. ABC_transpr_PstB.
IPR027417. P-loop_NTPase.
IPR005670. Phosp_transpt1.
[Graphical view]
PfamiPF00005. ABC_tran. 1 hit.
[Graphical view]
SMARTiSM00382. AAA. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR00972. 3a0107s01c2. 1 hit.
PROSITEiPS00211. ABC_TRANSPORTER_1. 1 hit.
PS50893. ABC_TRANSPORTER_2. 1 hit.
PS51238. PSTB. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiPSTB_MYCS2
AccessioniPrimary (citable) accession number: A0R4C0
Secondary accession number(s): I7GET6, O68469, Q7WTY5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 10, 2007
Last sequence update: January 9, 2007
Last modified: November 30, 2016
This is version 74 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.