A0R3N9 (G6PI_MYCS2) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Glucose-6-phosphate isomerase
Short name=GPI
EC=5.3.1.9

Alternative name(s):
Phosphoglucose isomerase
Short name=PGI
Phosphohexose isomerase
Short name=PHI

Gene names

Name:	pgi
Ordered Locus Names:	MSMEG_5541

Organism

Mycobacterium smegmatis (strain ATCC 700084 / mc(2)155)

Taxonomic identifier

246196 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Corynebacterineae › Mycobacteriaceae › Mycobacterium

Protein attributes

Sequence length	549 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Catalytic activity	D-glucose 6-phosphate = D-fructose 6-phosphate. HAMAP MF_00473
Pathway	Carbohydrate biosynthesis; gluconeogenesis. HAMAP MF_00473 Carbohydrate degradation; glycolysis; D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose: step 2/4. HAMAP MF_00473
Subcellular location	Cytoplasm By similarity HAMAP MF_00473.
Sequence similarities	Belongs to the GPI family.

Ontologies

Keywords
Biological process	Gluconeogenesis Glycolysis
Cellular component	Cytoplasm
Molecular function	Isomerase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	gluconeogenesis Inferred from electronic annotation. Source: UniProtKB-KW glycolysis Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	glucose-6-phosphate isomerase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 549	549	Glucose-6-phosphate isomerase HAMAP MF_00473		PRO_1000013989
Sites
	Active site	353	1	Proton donor By similarity
	Active site	384	1	By similarity
	Active site	510	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	A0R3N9 [UniParc]. Last modified January 9, 2007. Version 1. Checksum: E48F1BD4A53DCD73	FASTA	549	59,630
	10 20 30 40 50 60 MSADITETPA WQALSDHHAE IGDRHLTELF ADDPARGTEL ALTVGDLYID YSKHRVTRRT 70 80 90 100 110 120 LDLLVDLARA AGLEERRDAM FAGEHINTSE DRAVLHTALR LPRDAKLVVD GQDVVADVHD 130 140 150 160 170 180 VLDRMGDFTD RLRSGEWTGA TGERITTVVN IGIGGSDLGP VMVYDALRHY ADAGISARFV 190 200 210 220 230 240 SNVDPADLVA KLDGLEPAKT LFIVASKTFS TLETLTNATA ARRWLTDALG DAAVAKHFVA 250 260 270 280 290 300 VSTNKKLVDE FGINTDNMFG FWDWVGGRYS VDSAIGLSVM AVIGKERFAE FLAGFHIVDE 310 320 330 340 350 360 HFRTAPLHQN APALLGLIGL WYSNFFGAQS RAVLPYSNDL SRFAAYLQQL TMESNGKSVR 370 380 390 400 410 420 ADGTPVSTDT GEIFWGEPGT NGQHAFYQLL HQGTRLVPAD FIGFSQPTDD LPTADGTGSM 430 440 450 460 470 480 HDLLMSNFFA QTQVLAFGKT ADAIASEGTP ADVVPHKVMP GNRPTTSILA TKLTPSVVGQ 490 500 510 520 530 540 LIALYEHQVF TEGVIWGIDS FDQWGVELGK TQAKALLPVL TGDKSPAAQS DTSTDALVRR YRTERGRPA

References

[1]	Fleischmann R.D., Dodson R.J., Haft D.H., Merkel J.S., Nelson W.C., Fraser C.M. Submitted (OCT-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 700084 / mc(2)155.
[2]	"Ortho-proteogenomics: multiple proteomes investigation through orthology and a new MS-based protocol." Gallien S., Perrodou E., Carapito C., Deshayes C., Reyrat J.-M., Van Dorsselaer A., Poch O., Schaeffer C., Lecompte O. Genome Res. 19:128-135(2009) [PubMed: 18955433] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000480 Genomic DNA. Translation: ABK73757.1.
RefSeq	YP_889777.1. NC_008596.1.
3D structure databases
ProteinModelPortal	A0R3N9.
ModBase	Search...
Protein-protein interaction databases
STRING	A0R3N9.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBMYCT00000040757; EBMYCP00000039094; EBMYCG00000040752.
GeneID	4534436.
GenomeReviews	Gene locus MSMEG_5541 in contig CP000480_GR.
KEGG	msm:MSMEG_5541.
NMPDR	fig|246196.1.peg.5517.
PATRIC	18083347. VBIMycSme59918_5399.
TIGR	MSMEG_5541.
Phylogenomic databases
eggNOG	COG0166.
GeneTree	EBGT00050000016991.
HOGENOM	HBG352954.
OMA	NSLPAWT.
PhylomeDB	A0R3N9.
ProtClustDB	PRK00179.
Enzyme and pathway databases
BioCyc	MSME246196:MSMEG_5541-MONOMER.
Family and domain databases
HAMAP	MF_00473. G6P_isomerase. [Tree]
InterPro	IPR001672. G6P_Isomerase. IPR023096. G6P_Isomerase_C. IPR018189. Phosphoglucose_isomerase_CS. [Graphical view]
Gene3D	G3DSA:1.10.1390.10. G6P_Isomerase_C. 1 hit.
KO	K01810.
PANTHER	PTHR11469. G6P_Isomerase. 1 hit.
Pfam	PF00342. PGI. 1 hit. [Graphical view]
PRINTS	PR00662. G6PISOMERASE.
PROSITE	PS00765. P_GLUCOSE_ISOMERASE_1. 1 hit. PS00174. P_GLUCOSE_ISOMERASE_2. 1 hit. PS51463. P_GLUCOSE_ISOMERASE_3. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

G6PI_MYCS2

Accession

Primary (citable) accession number: A0R3N9

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	January 9, 2007
Last modified:	December 14, 2011
This is version 34 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program