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Protein

Intracellular chorismate mutase

Gene

MSMEG_5536

Organism
Mycobacterium smegmatis (strain ATCC 700084 / mc(2)155)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the Claisen rearrangement of chorismate to prephenate. Probably involved in the aromatic amino acid biosynthesis.1 Publication

Catalytic activityi

Chorismate = prephenate.

Pathwayi: prephenate biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes prephenate from chorismate.
Proteins known to be involved in this subpathway in this organism are:
  1. Intracellular chorismate mutase (MSMEG_5536), Secreted chorismate mutase (MSMEG_2111)
This subpathway is part of the pathway prephenate biosynthesis, which is itself part of Metabolic intermediate biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes prephenate from chorismate, the pathway prephenate biosynthesis and in Metabolic intermediate biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei31SubstrateSequence analysis1
Binding sitei48SubstrateBy similarity1
Binding sitei52SubstrateBy similarity1
Binding sitei59SubstrateSequence analysis1
Sitei59Important for catalysisBy similarity1
Sitei100Important for activation via AroGBy similarity1
Sitei101Important for activation via AroGBy similarity1
Sitei102Important for activation via AroGBy similarity1

GO - Molecular functioni

  • chorismate mutase activity Source: UniProtKB

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Isomerase

Keywords - Biological processi

Amino-acid biosynthesis, Aromatic amino acid biosynthesis

Enzyme and pathway databases

UniPathwayiUPA00120; UER00203.

Names & Taxonomyi

Protein namesi
Recommended name:
Intracellular chorismate mutase (EC:5.4.99.5)
Short name:
CM
Gene namesi
Ordered Locus Names:MSMEG_5536, MSMEI_5383
OrganismiMycobacterium smegmatis (strain ATCC 700084 / mc(2)155)
Taxonomic identifieri246196 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaCorynebacterialesMycobacteriaceaeMycobacterium
Proteomesi
  • UP000006158 Componenti: Chromosome
  • UP000000757 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00004149041 – 104Intracellular chorismate mutaseAdd BLAST104

Interactioni

Subunit structurei

Homodimer (By similarity). Probably interacts with MSMEG_4244.By similarity1 Publication

Protein-protein interaction databases

STRINGi246196.MSMEG_5536.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini23 – 104Chorismate mutasePROSITE-ProRule annotationAdd BLAST82

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni71 – 72Substrate bindingBy similarity2
Regioni99 – 100Substrate bindingSequence analysis2

Sequence similaritiesi

Contains 1 chorismate mutase domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiENOG4106D2I. Bacteria.
ENOG410XW4G. LUCA.
HOGENOMiHOG000019826.
KOiK04093.
OrthoDBiPOG091H0AWL.

Family and domain databases

Gene3Di1.20.59.10. 1 hit.
InterProiIPR002701. Chorismate_mutase.
IPR010958. Chorismate_mutase_highGC-bac.
IPR020822. Chorismate_mutase_type_II.
[Graphical view]
PfamiPF01817. CM_2. 1 hit.
[Graphical view]
SMARTiSM00830. CM_2. 1 hit.
[Graphical view]
SUPFAMiSSF48600. SSF48600. 1 hit.
TIGRFAMsiTIGR01808. CM_M_hiGC-arch. 1 hit.
PROSITEiPS51168. CHORISMATE_MUT_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

A0R3N5-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRPDHRMGPP HDEEPHMPET IDAVPEIDDL RREIDELDAT IIAAIQRRTE
60 70 80 90 100
VSKTIGKARM ASGGTRLVHS REMKVIERYI DALGPEGKDL AMLLLRLGRG

RLGY
Length:104
Mass (Da):11,754
Last modified:January 25, 2012 - v2
Checksum:iFE8C0C16F012CF91
GO

Sequence cautioni

The sequence ABK71866 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated
The sequence AFP41824 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000480 Genomic DNA. Translation: ABK71866.1. Different initiation.
CP001663 Genomic DNA. Translation: AFP41824.1. Different initiation.
RefSeqiYP_889773.1. NC_008596.1.

Genome annotation databases

EnsemblBacteriaiABK71866; ABK71866; MSMEG_5536.
AFP41824; AFP41824; MSMEI_5383.
GeneIDi4536816.
KEGGimsg:MSMEI_5383.
msm:MSMEG_5536.
PATRICi18083339. VBIMycSme59918_5395.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000480 Genomic DNA. Translation: ABK71866.1. Different initiation.
CP001663 Genomic DNA. Translation: AFP41824.1. Different initiation.
RefSeqiYP_889773.1. NC_008596.1.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi246196.MSMEG_5536.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABK71866; ABK71866; MSMEG_5536.
AFP41824; AFP41824; MSMEI_5383.
GeneIDi4536816.
KEGGimsg:MSMEI_5383.
msm:MSMEG_5536.
PATRICi18083339. VBIMycSme59918_5395.

Phylogenomic databases

eggNOGiENOG4106D2I. Bacteria.
ENOG410XW4G. LUCA.
HOGENOMiHOG000019826.
KOiK04093.
OrthoDBiPOG091H0AWL.

Enzyme and pathway databases

UniPathwayiUPA00120; UER00203.

Family and domain databases

Gene3Di1.20.59.10. 1 hit.
InterProiIPR002701. Chorismate_mutase.
IPR010958. Chorismate_mutase_highGC-bac.
IPR020822. Chorismate_mutase_type_II.
[Graphical view]
PfamiPF01817. CM_2. 1 hit.
[Graphical view]
SMARTiSM00830. CM_2. 1 hit.
[Graphical view]
SUPFAMiSSF48600. SSF48600. 1 hit.
TIGRFAMsiTIGR01808. CM_M_hiGC-arch. 1 hit.
PROSITEiPS51168. CHORISMATE_MUT_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiCHMU_MYCS2
AccessioniPrimary (citable) accession number: A0R3N5
Secondary accession number(s): I7GFF2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 25, 2012
Last sequence update: January 25, 2012
Last modified: November 2, 2016
This is version 60 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.