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Protein

Succinyl-CoA ligase [ADP-forming] subunit beta

Gene

sucC

Organism
Mycobacterium smegmatis (strain ATCC 700084 / mc(2)155)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

ATP + succinate + CoA = ADP + phosphate + succinyl-CoA.UniRule annotation

Cofactori

Mg2+UniRule annotation, Mn2+UniRule annotationNote: Binds 1 Mg2+ or Mn2+ ion per subunit.UniRule annotation

Pathwayi: tricarboxylic acid cycle

This protein is involved in step 1 of the subpathway that synthesizes succinate from succinyl-CoA (ligase route).UniRule annotation
Proteins known to be involved in this subpathway in this organism are:
  1. Succinyl-CoA ligase [ADP-forming] subunit beta (sucC)
This subpathway is part of the pathway tricarboxylic acid cycle, which is itself part of Carbohydrate metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes succinate from succinyl-CoA (ligase route), the pathway tricarboxylic acid cycle and in Carbohydrate metabolism.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi189 – 1891Magnesium or manganeseUniRule annotation
Metal bindingi191 – 1911Magnesium or manganeseUniRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi35 – 10066ATPUniRule annotationAdd
BLAST

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Biological processi

Tricarboxylic acid cycle

Keywords - Ligandi

ATP-binding, Magnesium, Manganese, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciMSME246196:GJ4Y-5524-MONOMER.
UniPathwayiUPA00223; UER00999.

Names & Taxonomyi

Protein namesi
Recommended name:
Succinyl-CoA ligase [ADP-forming] subunit betaUniRule annotation (EC:6.2.1.5UniRule annotation)
Alternative name(s):
Succinyl-CoA synthetase subunit betaUniRule annotation
Short name:
SCS-betaUniRule annotation
Gene namesi
Name:sucCUniRule annotation
Ordered Locus Names:MSMEG_5525, MSMEI_5373
OrganismiMycobacterium smegmatis (strain ATCC 700084 / mc(2)155)
Taxonomic identifieri246196 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaCorynebacterialesMycobacteriaceaeMycobacterium
Proteomesi
  • UP000006158 Componenti: Chromosome
  • UP000000757 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 387387Succinyl-CoA ligase [ADP-forming] subunit betaPRO_1000082128Add
BLAST

Interactioni

Subunit structurei

Heterotetramer of two alpha and two beta subunits.UniRule annotation

Protein-protein interaction databases

STRINGi246196.MSMEG_5525.

Structurei

3D structure databases

ProteinModelPortaliA0R3M4.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini9 – 236228ATP-graspUniRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the succinate/malate CoA ligase beta subunit family.UniRule annotation
Contains 1 ATP-grasp domain.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105CMV. Bacteria.
COG0045. LUCA.
HOGENOMiHOG000007059.
KOiK01903.
OMAiYIESGCD.
OrthoDBiEOG644ZT0.

Family and domain databases

Gene3Di3.30.1490.20. 1 hit.
3.30.470.20. 1 hit.
3.40.50.261. 1 hit.
HAMAPiMF_00558. Succ_CoA_beta.
InterProiIPR011761. ATP-grasp.
IPR013650. ATP-grasp_succ-CoA_synth-type.
IPR013815. ATP_grasp_subdomain_1.
IPR013816. ATP_grasp_subdomain_2.
IPR005811. CoA_ligase.
IPR017866. Succ-CoA_synthase_bsu_CS.
IPR005809. Succ_CoA_synthase_bsu.
IPR016102. Succinyl-CoA_synth-like.
[Graphical view]
PANTHERiPTHR11815. PTHR11815. 1 hit.
PfamiPF08442. ATP-grasp_2. 1 hit.
PF00549. Ligase_CoA. 1 hit.
[Graphical view]
PIRSFiPIRSF001554. SucCS_beta. 1 hit.
SUPFAMiSSF52210. SSF52210. 1 hit.
TIGRFAMsiTIGR01016. sucCoAbeta. 1 hit.
PROSITEiPS50975. ATP_GRASP. 1 hit.
PS01217. SUCCINYL_COA_LIG_3. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

A0R3M4-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDLFEYQAKE LFAKHNVPTS PGRVTDSAED AKTIAEEIGR PVMVKAQVKT
60 70 80 90 100
GGRGKAGGVK YAATPDDAFT HANNILGLDI KGHVVKKLLV AEASDIAEEY
110 120 130 140 150
YISFLLDRAN RTYLAMCSVE GGMEIEEVAA TKPERLAKVP VDAVKGVDLA
160 170 180 190 200
FARSIAEQGH LPAEVLDAAA VTIQKLWEVF VKEDATLVEV NPLVRTPDDQ
210 220 230 240 250
ILALDGKVTL DENAGFRQPG HAEFEDRDAT DPLELKAKEN DLNYVKLDGQ
260 270 280 290 300
VGIIGNGAGL VMSTLDVVAY AGENHGGVKP ANFLDIGGGA SAAVMAAGLD
310 320 330 340 350
VILGDSQVKS VFVNVFGGIT ACDAVANGIV QALQILGDEA NKPLVVRLDG
360 370 380
NNVEEGRRIL AEANHPLVIQ AETMDAGADK AAELANK
Length:387
Mass (Da):40,932
Last modified:January 9, 2007 - v1
Checksum:iB77A4AEFBD640D07
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000480 Genomic DNA. Translation: ABK71969.1.
CP001663 Genomic DNA. Translation: AFP41814.1.
RefSeqiWP_011730595.1. NZ_CP009494.1.
YP_889762.1. NC_008596.1.

Genome annotation databases

EnsemblBacteriaiABK71969; ABK71969; MSMEG_5525.
AFP41814; AFP41814; MSMEI_5373.
GeneIDi4535810.
KEGGimsb:LJ00_27315.
msg:MSMEI_5373.
msm:MSMEG_5525.
PATRICi18083319. VBIMycSme59918_5385.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000480 Genomic DNA. Translation: ABK71969.1.
CP001663 Genomic DNA. Translation: AFP41814.1.
RefSeqiWP_011730595.1. NZ_CP009494.1.
YP_889762.1. NC_008596.1.

3D structure databases

ProteinModelPortaliA0R3M4.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi246196.MSMEG_5525.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABK71969; ABK71969; MSMEG_5525.
AFP41814; AFP41814; MSMEI_5373.
GeneIDi4535810.
KEGGimsb:LJ00_27315.
msg:MSMEI_5373.
msm:MSMEG_5525.
PATRICi18083319. VBIMycSme59918_5385.

Phylogenomic databases

eggNOGiENOG4105CMV. Bacteria.
COG0045. LUCA.
HOGENOMiHOG000007059.
KOiK01903.
OMAiYIESGCD.
OrthoDBiEOG644ZT0.

Enzyme and pathway databases

UniPathwayiUPA00223; UER00999.
BioCyciMSME246196:GJ4Y-5524-MONOMER.

Family and domain databases

Gene3Di3.30.1490.20. 1 hit.
3.30.470.20. 1 hit.
3.40.50.261. 1 hit.
HAMAPiMF_00558. Succ_CoA_beta.
InterProiIPR011761. ATP-grasp.
IPR013650. ATP-grasp_succ-CoA_synth-type.
IPR013815. ATP_grasp_subdomain_1.
IPR013816. ATP_grasp_subdomain_2.
IPR005811. CoA_ligase.
IPR017866. Succ-CoA_synthase_bsu_CS.
IPR005809. Succ_CoA_synthase_bsu.
IPR016102. Succinyl-CoA_synth-like.
[Graphical view]
PANTHERiPTHR11815. PTHR11815. 1 hit.
PfamiPF08442. ATP-grasp_2. 1 hit.
PF00549. Ligase_CoA. 1 hit.
[Graphical view]
PIRSFiPIRSF001554. SucCS_beta. 1 hit.
SUPFAMiSSF52210. SSF52210. 1 hit.
TIGRFAMsiTIGR01016. sucCoAbeta. 1 hit.
PROSITEiPS50975. ATP_GRASP. 1 hit.
PS01217. SUCCINYL_COA_LIG_3. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Fleischmann R.D., Dodson R.J., Haft D.H., Merkel J.S., Nelson W.C., Fraser C.M.
    Submitted (OCT-2006) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 700084 / mc(2)155.
  2. "Interrupted coding sequences in Mycobacterium smegmatis: authentic mutations or sequencing errors?"
    Deshayes C., Perrodou E., Gallien S., Euphrasie D., Schaeffer C., Van-Dorsselaer A., Poch O., Lecompte O., Reyrat J.-M.
    Genome Biol. 8:R20.1-R20.9(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 700084 / mc(2)155.
  3. "Ortho-proteogenomics: multiple proteomes investigation through orthology and a new MS-based protocol."
    Gallien S., Perrodou E., Carapito C., Deshayes C., Reyrat J.-M., Van Dorsselaer A., Poch O., Schaeffer C., Lecompte O.
    Genome Res. 19:128-135(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS], IDENTIFICATION OF N-TERMINUS.
    Strain: ATCC 700084 / mc(2)155.

Entry informationi

Entry nameiSUCC_MYCS2
AccessioniPrimary (citable) accession number: A0R3M4
Secondary accession number(s): I7GFE8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 20, 2008
Last sequence update: January 9, 2007
Last modified: November 11, 2015
This is version 73 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.