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Protein

Response regulator MprA

Gene

mprA

Organism
Mycobacterium smegmatis (strain ATCC 700084 / mc(2)155)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Experimental evidence at protein leveli

Functioni

Member of the two-component regulatory system MprB/MprA which contributes to maintaining a balance among several systems involved in stress resistance and is required for establishment and maintenance of persistent infection in the host. Functions as a transcriptional regulator that recognizes a 19-bp nucleotide motif comprizing two loosely conserved 8-bp direct DNA-binding motif repeats separated by a 3-bp spacer region (By similarity).By similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Stress response, Transcription, Transcription regulation, Two-component regulatory system, Virulence

Keywords - Ligandi

DNA-binding

Enzyme and pathway databases

BioCyciMSME246196:GJ4Y-5487-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Response regulator MprA
Alternative name(s):
Mycobacterial persistence regulator A
Gene namesi
Name:mprA
Ordered Locus Names:MSMEG_5488, MSMEI_5336
OrganismiMycobacterium smegmatis (strain ATCC 700084 / mc(2)155)
Taxonomic identifieri246196 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaActinobacteridaeActinomycetalesCorynebacterineaeMycobacteriaceaeMycobacterium
ProteomesiUP000006158 Componenti: Chromosome UP000000757 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 230230Response regulator MprAPRO_0000308423Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei48 – 4814-aspartylphosphatePROSITE-ProRule annotation

Post-translational modificationi

Phosphorylated and dephosphorylated by MprB.By similarity

Keywords - PTMi

Phosphoprotein

Interactioni

Protein-protein interaction databases

STRINGi246196.MSMEG_5488.

Structurei

3D structure databases

ProteinModelPortaliA0R3I8.
SMRiA0R3I8. Positions 3-227.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini4 – 118115Response regulatoryPROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Contains 1 response regulatory domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiCOG0745.
HOGENOMiHOG000034815.
KOiK07669.
OMAiFTLLEMF.
OrthoDBiEOG6G4VQG.

Family and domain databases

Gene3Di1.10.10.10. 1 hit.
InterProiIPR011006. CheY-like_superfamily.
IPR001867. Sig_transdc_resp-reg_C.
IPR001789. Sig_transdc_resp-reg_receiver.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view]
PfamiPF00072. Response_reg. 1 hit.
PF00486. Trans_reg_C. 1 hit.
[Graphical view]
SMARTiSM00448. REC. 1 hit.
SM00862. Trans_reg_C. 1 hit.
[Graphical view]
SUPFAMiSSF52172. SSF52172. 1 hit.
PROSITEiPS50110. RESPONSE_REGULATORY. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

A0R3I8-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAVRILVVDD DRAVRESLRR SLSFNGYSVE LAQDGVEALD AITNNRPDAL
60 70 80 90 100
ILDVMMPRLD GLEVCRQLRS TGDDLPILVL TARDSVSERV AGLDAGADDY
110 120 130 140 150
LPKPFALEEL LARMRALLRR TVSDDSGDSQ KMTFSDLTLD PVTREVTRGG
160 170 180 190 200
RQISLTRTEF SLLEMLIANP RRVLTRSRIL EEVWGFDFPT SGNALEVYIG
210 220 230
YLRRKTEAEG EPRLIHTVRG VGYVLRETPP
Length:230
Mass (Da):25,774
Last modified:January 9, 2007 - v1
Checksum:i7C967F0D16BD7301
GO

Sequence cautioni

The sequence AFP41777.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000480 Genomic DNA. Translation: ABK75202.1.
CP001663 Genomic DNA. Translation: AFP41777.1. Different initiation.
RefSeqiWP_011730567.1. NC_008596.1.
YP_006570072.1. NC_018289.1.
YP_889726.1. NC_008596.1.

Genome annotation databases

EnsemblBacteriaiABK75202; ABK75202; MSMEG_5488.
AFP41777; AFP41777; MSMEI_5336.
GeneIDi4532340.
KEGGimsm:MSMEG_5488.
PATRICi18083245. VBIMycSme59918_5348.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000480 Genomic DNA. Translation: ABK75202.1.
CP001663 Genomic DNA. Translation: AFP41777.1. Different initiation.
RefSeqiWP_011730567.1. NC_008596.1.
YP_006570072.1. NC_018289.1.
YP_889726.1. NC_008596.1.

3D structure databases

ProteinModelPortaliA0R3I8.
SMRiA0R3I8. Positions 3-227.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi246196.MSMEG_5488.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABK75202; ABK75202; MSMEG_5488.
AFP41777; AFP41777; MSMEI_5336.
GeneIDi4532340.
KEGGimsm:MSMEG_5488.
PATRICi18083245. VBIMycSme59918_5348.

Phylogenomic databases

eggNOGiCOG0745.
HOGENOMiHOG000034815.
KOiK07669.
OMAiFTLLEMF.
OrthoDBiEOG6G4VQG.

Enzyme and pathway databases

BioCyciMSME246196:GJ4Y-5487-MONOMER.

Family and domain databases

Gene3Di1.10.10.10. 1 hit.
InterProiIPR011006. CheY-like_superfamily.
IPR001867. Sig_transdc_resp-reg_C.
IPR001789. Sig_transdc_resp-reg_receiver.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view]
PfamiPF00072. Response_reg. 1 hit.
PF00486. Trans_reg_C. 1 hit.
[Graphical view]
SMARTiSM00448. REC. 1 hit.
SM00862. Trans_reg_C. 1 hit.
[Graphical view]
SUPFAMiSSF52172. SSF52172. 1 hit.
PROSITEiPS50110. RESPONSE_REGULATORY. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Fleischmann R.D., Dodson R.J., Haft D.H., Merkel J.S., Nelson W.C., Fraser C.M.
    Submitted (OCT-2006) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 700084 / mc(2)155.
  2. "Interrupted coding sequences in Mycobacterium smegmatis: authentic mutations or sequencing errors?"
    Deshayes C., Perrodou E., Gallien S., Euphrasie D., Schaeffer C., Van-Dorsselaer A., Poch O., Lecompte O., Reyrat J.-M.
    Genome Biol. 8:R20.1-R20.9(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 700084 / mc(2)155.
  3. "Ortho-proteogenomics: multiple proteomes investigation through orthology and a new MS-based protocol."
    Gallien S., Perrodou E., Carapito C., Deshayes C., Reyrat J.-M., Van Dorsselaer A., Poch O., Schaeffer C., Lecompte O.
    Genome Res. 19:128-135(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Strain: ATCC 700084 / mc(2)155.

Entry informationi

Entry nameiMPRA_MYCS2
AccessioniPrimary (citable) accession number: A0R3I8
Secondary accession number(s): I7G7T7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 23, 2007
Last sequence update: January 9, 2007
Last modified: April 29, 2015
This is version 67 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.