A0R3D7 (ISPE_MYCS2) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
4-diphosphocytidyl-2-C-methyl-D-erythritol kinase
Short name=CMK
EC=2.7.1.148

Alternative name(s):
4-(cytidine-5'-diphospho)-2-C-methyl-D-erythritol kinase

Gene names

Name:	ispE
Ordered Locus Names:	MSMEG_5436

Organism

Mycobacterium smegmatis (strain ATCC 700084 / mc(2)155)

Taxonomic identifier

246196 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Corynebacterineae › Mycobacteriaceae › Mycobacterium

Protein attributes

Sequence length	322 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the phosphorylation of the position 2 hydroxy group of 4-diphosphocytidyl-2C-methyl-D-erythritol By similarity. HAMAP MF_00061
Catalytic activity	ATP + 4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol = ADP + 2-phospho-4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol. HAMAP MF_00061
Pathway	Isoprenoid biosynthesis; isopentenyl diphosphate biosynthesis via DXP pathway; isopentenyl diphosphate from 1-deoxy-D-xylulose 5-phosphate: step 3/6. HAMAP MF_00061
Sequence similarities	Belongs to the GHMP kinase family. IspE subfamily.

Ontologies

Keywords
Biological process	Isoprene biosynthesis
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	terpenoid biosynthetic process Inferred from electronic annotation. Source: InterPro
Molecular function	4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol kinase activity Inferred from electronic annotation. Source: EC ATP binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 322	322	4-diphosphocytidyl-2-C-methyl-D-erythritol kinase HAMAP MF_00061		PRO_0000335730
Regions
	Nucleotide binding	112 – 122	11	ATP Potential
Sites
	Active site	27	1	By similarity
	Active site	154	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	A0R3D7 [UniParc]. Last modified January 9, 2007. Version 1. Checksum: 9004AAA111E2164B	FASTA	322	33,034
	10 20 30 40 50 60 MAVPASDGST ASEWVTTGSV TVRVPGKVNL YLSVGDLRDD GYHDLTTVFH AVSLLDEVTV 70 80 90 100 110 120 RNADVLSLRM IGEGAESLPA DERNLAWRAA ELMAEHVGRA PDVEITIEKS IPVAGGMAGG 130 140 150 160 170 180 SADAAGVLVA MNTLWELGVP RRDLHALAAQ LGSDVPFALH GGTALGSGRG EDLATVLARN 190 200 210 220 230 240 TFHWVLAFAH RGLSTPKVFG ELDRLRADTT RGLPRTDEPE PVLAALASGD PAELAALLGN 250 260 270 280 290 300 DLQPAALSLY PDLRRTLRAG VEAGALAGIV SGSGPTCAFL CASAPAALDV GTQLSGAGVC 310 320 RTVRVASGPV HGAHVVPAPS AS

References

[1]	Fleischmann R.D., Dodson R.J., Haft D.H., Merkel J.S., Nelson W.C., Fraser C.M. Submitted (OCT-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 700084 / mc(2)155.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000480 Genomic DNA. Translation: ABK71884.1.
RefSeq	YP_889675.1. NC_008596.1.
3D structure databases
ProteinModelPortal	A0R3D7.
ModBase	Search...
Protein-protein interaction databases
STRING	A0R3D7.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBMYCT00000045656; EBMYCP00000043993; EBMYCG00000045651.
GeneID	4537206.
GenomeReviews	Gene locus MSMEG_5436 in contig CP000480_GR.
KEGG	msm:MSMEG_5436.
NMPDR	fig|246196.1.peg.5416.
PATRIC	18083141. VBIMycSme59918_5297.
TIGR	MSMEG_5436.
Phylogenomic databases
eggNOG	COG1947.
GeneTree	EBGT00050000016146.
HOGENOM	HBG734593.
OMA	KINLHLG.
PhylomeDB	A0R3D7.
ProtClustDB	PRK03188.
Enzyme and pathway databases
BioCyc	MSME246196:MSMEG_5436-MONOMER.
Family and domain databases
HAMAP	MF_00061. IspE. [Tree]
InterPro	IPR006204. GHMP_kinase. IPR013750. GHMP_kinase_C. IPR004424. IspE. IPR020568. Ribosomal_S5_D2-typ_fold. IPR014721. Ribosomal_S5_D2-typ_fold_subgr. [Graphical view]
Gene3D	G3DSA:3.30.230.10. Ribosomal_S5_D2-type_fold. 1 hit.
KO	K00919.
PANTHER	PTHR20861:SF2. IspE. 1 hit.
Pfam	PF08544. GHMP_kinases_C. 1 hit. PF00288. GHMP_kinases_N. 1 hit. [Graphical view]
PIRSF	PIRSF010376. IspE. 1 hit.
SUPFAM	SSF54211. Ribosomal_S5_D2-typ_fold. 1 hit.
TIGRFAMs	TIGR00154. IspE. 1 hit.
ProtoNet	Search...

Entry information

Entry name

ISPE_MYCS2

Accession

Primary (citable) accession number: A0R3D7

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 20, 2008
Last sequence update:	January 9, 2007
Last modified:	December 14, 2011
This is version 40 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program