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Protein

Peptidyl-tRNA hydrolase

Gene

pth

Organism
Mycobacterium smegmatis (strain ATCC 700084 / mc(2)155)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Experimental evidence at protein leveli

Functioni

The natural substrate for this enzyme may be peptidyl-tRNAs which drop off the ribosome during protein synthesis.UniRule annotation

Catalytic activityi

N-substituted aminoacyl-tRNA + H2O = N-substituted amino acid + tRNA.UniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Enzyme and pathway databases

BioCyciMSME246196:GJ4Y-5431-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Peptidyl-tRNA hydrolaseUniRule annotation (EC:3.1.1.29UniRule annotation)
Short name:
PTHUniRule annotation
Gene namesi
Name:pthUniRule annotation
Ordered Locus Names:MSMEG_5432, MSMEI_5283
OrganismiMycobacterium smegmatis (strain ATCC 700084 / mc(2)155)
Taxonomic identifieri246196 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaCorynebacterialesMycobacteriaceaeMycobacterium
Proteomesi
  • UP000006158 Componenti: Chromosome
  • UP000000757 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 191191Peptidyl-tRNA hydrolasePRO_1000010613Add
BLAST

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi246196.MSMEG_5432.

Structurei

Secondary structure

1
191
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi5 – 95Combined sources
Turni15 – 195Combined sources
Helixi21 – 233Combined sources
Helixi24 – 3613Combined sources
Beta strandi41 – 433Combined sources
Turni44 – 463Combined sources
Beta strandi49 – 557Combined sources
Beta strandi58 – 647Combined sources
Helixi69 – 724Combined sources
Helixi73 – 8210Combined sources
Helixi87 – 893Combined sources
Beta strandi90 – 9910Combined sources
Beta strandi104 – 1107Combined sources
Helixi116 – 12510Combined sources
Beta strandi130 – 1367Combined sources
Beta strandi141 – 1433Combined sources
Helixi146 – 1494Combined sources
Helixi156 – 1594Combined sources
Helixi162 – 17918Combined sources
Helixi181 – 1888Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2LGJNMR-A1-191[»]
3KJZX-ray2.40A1-191[»]
3KK0X-ray2.65A1-191[»]
3P2JX-ray2.22A1-191[»]
ProteinModelPortaliA0R3D3.
SMRiA0R3D3. Positions 1-191.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiA0R3D3.

Family & Domainsi

Sequence similaritiesi

Belongs to the PTH family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4108ZPD. Bacteria.
COG0193. LUCA.
HOGENOMiHOG000004796.
KOiK01056.
OMAiNGLKSMT.
OrthoDBiEOG6C5RTR.

Family and domain databases

Gene3Di3.40.50.1470. 1 hit.
HAMAPiMF_00083. Pept_tRNA_hydro_bact.
InterProiIPR001328. Pept_tRNA_hydro.
IPR018171. Pept_tRNA_hydro_CS.
[Graphical view]
PANTHERiPTHR17224. PTHR17224. 1 hit.
PfamiPF01195. Pept_tRNA_hydro. 1 hit.
[Graphical view]
SUPFAMiSSF53178. SSF53178. 1 hit.
TIGRFAMsiTIGR00447. pth. 1 hit.
PROSITEiPS01195. PEPT_TRNA_HYDROL_1. 1 hit.
PS01196. PEPT_TRNA_HYDROL_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

A0R3D3-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAEPLLVVGL GNPGPTYAKT RHNLGFMVAD VLAGRIGSAF KVHKKSGAEV
60 70 80 90 100
VTGRLAGTSV VLAKPRCYMN ESGRQVGPLA KFYSVPPQQI VVIHDELDID
110 120 130 140 150
FGRIRLKLGG GEGGHNGLRS VASALGTKNF HRVRIGVGRP PGRKDPAAFV
160 170 180 190
LENFTAAERA EVPTIVEQAA DATELLIAQG LEPAQNTVHA W
Length:191
Mass (Da):20,278
Last modified:January 9, 2007 - v1
Checksum:iBA77A2644BDD6419
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000480 Genomic DNA. Translation: ABK75581.1.
CP001663 Genomic DNA. Translation: AFP41725.1.
RefSeqiWP_011730530.1. NZ_CP009494.1.
YP_889671.1. NC_008596.1.

Genome annotation databases

EnsemblBacteriaiABK75581; ABK75581; MSMEG_5432.
AFP41725; AFP41725; MSMEI_5283.
GeneIDi4537016.
KEGGimsb:LJ00_26845.
msg:MSMEI_5283.
msm:MSMEG_5432.
PATRICi18083133. VBIMycSme59918_5293.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000480 Genomic DNA. Translation: ABK75581.1.
CP001663 Genomic DNA. Translation: AFP41725.1.
RefSeqiWP_011730530.1. NZ_CP009494.1.
YP_889671.1. NC_008596.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2LGJNMR-A1-191[»]
3KJZX-ray2.40A1-191[»]
3KK0X-ray2.65A1-191[»]
3P2JX-ray2.22A1-191[»]
ProteinModelPortaliA0R3D3.
SMRiA0R3D3. Positions 1-191.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi246196.MSMEG_5432.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABK75581; ABK75581; MSMEG_5432.
AFP41725; AFP41725; MSMEI_5283.
GeneIDi4537016.
KEGGimsb:LJ00_26845.
msg:MSMEI_5283.
msm:MSMEG_5432.
PATRICi18083133. VBIMycSme59918_5293.

Phylogenomic databases

eggNOGiENOG4108ZPD. Bacteria.
COG0193. LUCA.
HOGENOMiHOG000004796.
KOiK01056.
OMAiNGLKSMT.
OrthoDBiEOG6C5RTR.

Enzyme and pathway databases

BioCyciMSME246196:GJ4Y-5431-MONOMER.

Miscellaneous databases

EvolutionaryTraceiA0R3D3.

Family and domain databases

Gene3Di3.40.50.1470. 1 hit.
HAMAPiMF_00083. Pept_tRNA_hydro_bact.
InterProiIPR001328. Pept_tRNA_hydro.
IPR018171. Pept_tRNA_hydro_CS.
[Graphical view]
PANTHERiPTHR17224. PTHR17224. 1 hit.
PfamiPF01195. Pept_tRNA_hydro. 1 hit.
[Graphical view]
SUPFAMiSSF53178. SSF53178. 1 hit.
TIGRFAMsiTIGR00447. pth. 1 hit.
PROSITEiPS01195. PEPT_TRNA_HYDROL_1. 1 hit.
PS01196. PEPT_TRNA_HYDROL_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Fleischmann R.D., Dodson R.J., Haft D.H., Merkel J.S., Nelson W.C., Fraser C.M.
    Submitted (OCT-2006) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 700084 / mc(2)155.
  2. "Interrupted coding sequences in Mycobacterium smegmatis: authentic mutations or sequencing errors?"
    Deshayes C., Perrodou E., Gallien S., Euphrasie D., Schaeffer C., Van-Dorsselaer A., Poch O., Lecompte O., Reyrat J.-M.
    Genome Biol. 8:R20.1-R20.9(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 700084 / mc(2)155.
  3. "Ortho-proteogenomics: multiple proteomes investigation through orthology and a new MS-based protocol."
    Gallien S., Perrodou E., Carapito C., Deshayes C., Reyrat J.-M., Van Dorsselaer A., Poch O., Schaeffer C., Lecompte O.
    Genome Res. 19:128-135(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 700084 / mc(2)155.

Entry informationi

Entry nameiPTH_MYCS2
AccessioniPrimary (citable) accession number: A0R3D3
Secondary accession number(s): I7FK76
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: January 9, 2007
Last modified: November 11, 2015
This is version 70 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.