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Protein

Peptidyl-tRNA hydrolase

Gene

pth

Organism
Mycobacterium smegmatis (strain ATCC 700084 / mc(2)155)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Experimental evidence at protein leveli

Functioni

The natural substrate for this enzyme may be peptidyl-tRNAs which drop off the ribosome during protein synthesis.UniRule annotation

Catalytic activityi

N-substituted aminoacyl-tRNA + H2O = N-substituted amino acid + tRNA.UniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Names & Taxonomyi

Protein namesi
Recommended name:
Peptidyl-tRNA hydrolaseUniRule annotation (EC:3.1.1.29UniRule annotation)
Short name:
PTHUniRule annotation
Gene namesi
Name:pthUniRule annotation
Ordered Locus Names:MSMEG_5432, MSMEI_5283
OrganismiMycobacterium smegmatis (strain ATCC 700084 / mc(2)155)
Taxonomic identifieri246196 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaCorynebacterialesMycobacteriaceaeMycobacterium
Proteomesi
  • UP000006158 Componenti: Chromosome
  • UP000000757 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_10000106131 – 191Peptidyl-tRNA hydrolaseAdd BLAST191

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi246196.MSMEG_5432.

Structurei

Secondary structure

1191
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi5 – 9Combined sources5
Turni15 – 19Combined sources5
Helixi21 – 23Combined sources3
Helixi24 – 36Combined sources13
Beta strandi41 – 43Combined sources3
Turni44 – 46Combined sources3
Beta strandi49 – 55Combined sources7
Beta strandi58 – 64Combined sources7
Helixi69 – 72Combined sources4
Helixi73 – 82Combined sources10
Helixi87 – 89Combined sources3
Beta strandi90 – 99Combined sources10
Beta strandi104 – 110Combined sources7
Helixi116 – 125Combined sources10
Beta strandi130 – 136Combined sources7
Beta strandi141 – 143Combined sources3
Helixi146 – 149Combined sources4
Helixi156 – 159Combined sources4
Helixi162 – 179Combined sources18
Helixi181 – 188Combined sources8

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2LGJNMR-A1-191[»]
3KJZX-ray2.40A1-191[»]
3KK0X-ray2.65A1-191[»]
3P2JX-ray2.22A1-191[»]
ProteinModelPortaliA0R3D3.
SMRiA0R3D3.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiA0R3D3.

Family & Domainsi

Sequence similaritiesi

Belongs to the PTH family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4108ZPD. Bacteria.
COG0193. LUCA.
HOGENOMiHOG000004796.
KOiK01056.
OMAiNGLKSMT.
OrthoDBiPOG091H02CU.

Family and domain databases

CDDicd00462. PTH. 1 hit.
Gene3Di3.40.50.1470. 1 hit.
HAMAPiMF_00083. Pept_tRNA_hydro_bact. 1 hit.
InterProiIPR001328. Pept_tRNA_hydro.
IPR018171. Pept_tRNA_hydro_CS.
[Graphical view]
PANTHERiPTHR17224. PTHR17224. 1 hit.
PfamiPF01195. Pept_tRNA_hydro. 1 hit.
[Graphical view]
SUPFAMiSSF53178. SSF53178. 1 hit.
TIGRFAMsiTIGR00447. pth. 1 hit.
PROSITEiPS01195. PEPT_TRNA_HYDROL_1. 1 hit.
PS01196. PEPT_TRNA_HYDROL_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

A0R3D3-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAEPLLVVGL GNPGPTYAKT RHNLGFMVAD VLAGRIGSAF KVHKKSGAEV
60 70 80 90 100
VTGRLAGTSV VLAKPRCYMN ESGRQVGPLA KFYSVPPQQI VVIHDELDID
110 120 130 140 150
FGRIRLKLGG GEGGHNGLRS VASALGTKNF HRVRIGVGRP PGRKDPAAFV
160 170 180 190
LENFTAAERA EVPTIVEQAA DATELLIAQG LEPAQNTVHA W
Length:191
Mass (Da):20,278
Last modified:January 9, 2007 - v1
Checksum:iBA77A2644BDD6419
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000480 Genomic DNA. Translation: ABK75581.1.
CP001663 Genomic DNA. Translation: AFP41725.1.
RefSeqiWP_011730530.1. NZ_CP009494.1.
YP_889671.1. NC_008596.1.

Genome annotation databases

EnsemblBacteriaiABK75581; ABK75581; MSMEG_5432.
AFP41725; AFP41725; MSMEI_5283.
GeneIDi4537016.
KEGGimsb:LJ00_26845.
msg:MSMEI_5283.
msm:MSMEG_5432.
PATRICi18083133. VBIMycSme59918_5293.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000480 Genomic DNA. Translation: ABK75581.1.
CP001663 Genomic DNA. Translation: AFP41725.1.
RefSeqiWP_011730530.1. NZ_CP009494.1.
YP_889671.1. NC_008596.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2LGJNMR-A1-191[»]
3KJZX-ray2.40A1-191[»]
3KK0X-ray2.65A1-191[»]
3P2JX-ray2.22A1-191[»]
ProteinModelPortaliA0R3D3.
SMRiA0R3D3.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi246196.MSMEG_5432.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABK75581; ABK75581; MSMEG_5432.
AFP41725; AFP41725; MSMEI_5283.
GeneIDi4537016.
KEGGimsb:LJ00_26845.
msg:MSMEI_5283.
msm:MSMEG_5432.
PATRICi18083133. VBIMycSme59918_5293.

Phylogenomic databases

eggNOGiENOG4108ZPD. Bacteria.
COG0193. LUCA.
HOGENOMiHOG000004796.
KOiK01056.
OMAiNGLKSMT.
OrthoDBiPOG091H02CU.

Miscellaneous databases

EvolutionaryTraceiA0R3D3.

Family and domain databases

CDDicd00462. PTH. 1 hit.
Gene3Di3.40.50.1470. 1 hit.
HAMAPiMF_00083. Pept_tRNA_hydro_bact. 1 hit.
InterProiIPR001328. Pept_tRNA_hydro.
IPR018171. Pept_tRNA_hydro_CS.
[Graphical view]
PANTHERiPTHR17224. PTHR17224. 1 hit.
PfamiPF01195. Pept_tRNA_hydro. 1 hit.
[Graphical view]
SUPFAMiSSF53178. SSF53178. 1 hit.
TIGRFAMsiTIGR00447. pth. 1 hit.
PROSITEiPS01195. PEPT_TRNA_HYDROL_1. 1 hit.
PS01196. PEPT_TRNA_HYDROL_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiPTH_MYCS2
AccessioniPrimary (citable) accession number: A0R3D3
Secondary accession number(s): I7FK76
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: January 9, 2007
Last modified: November 2, 2016
This is version 73 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.