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A0R220

- THRC_MYCS2

UniProt

A0R220 - THRC_MYCS2

Protein

Threonine synthase

Gene

thrC

Organism
Mycobacterium smegmatis (strain ATCC 700084 / mc(2)155)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 57 (01 Oct 2014)
      Sequence version 1 (09 Jan 2007)
      Previous versions | rss
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    Functioni

    Catalyzes the gamma-elimination of phosphate from L-phosphohomoserine and the beta-addition of water to produce L-threonine.By similarity

    Catalytic activityi

    O-phospho-L-homoserine + H2O = L-threonine + phosphate.

    Cofactori

    Pyridoxal phosphate.By similarity

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei95 – 951Pyridoxal phosphateBy similarity
    Binding sitei326 – 3261Pyridoxal phosphateBy similarity

    GO - Molecular functioni

    1. pyridoxal phosphate binding Source: InterPro
    2. threonine synthase activity Source: UniProtKB-EC

    GO - Biological processi

    1. threonine biosynthetic process Source: UniProtKB-UniPathway

    Keywords - Molecular functioni

    Lyase

    Keywords - Biological processi

    Amino-acid biosynthesis, Threonine biosynthesis

    Keywords - Ligandi

    Pyridoxal phosphate

    Enzyme and pathway databases

    BioCyciMSME246196:GJ4Y-4955-MONOMER.
    UniPathwayiUPA00050; UER00065.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Threonine synthase (EC:4.2.3.1)
    Short name:
    TS
    Gene namesi
    Name:thrC
    Ordered Locus Names:MSMEG_4956, MSMEI_4829
    OrganismiMycobacterium smegmatis (strain ATCC 700084 / mc(2)155)
    Taxonomic identifieri246196 [NCBI]
    Taxonomic lineageiBacteriaActinobacteriaActinobacteridaeActinomycetalesCorynebacterineaeMycobacteriaceaeMycobacterium
    ProteomesiUP000000757: Chromosome, UP000006158: Chromosome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 360360Threonine synthasePRO_0000396139Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei69 – 691N6-(pyridoxal phosphate)lysineBy similarity
    Cross-linki151 – 151Isoglutamyl lysine isopeptide (Lys-Gln) (interchain with Q-Cter in protein Pup)1 Publication

    Keywords - PTMi

    Isopeptide bond, Ubl conjugation

    Interactioni

    Subunit structurei

    Homodimer.By similarity

    Protein-protein interaction databases

    STRINGi246196.MSMEG_4956.

    Structurei

    3D structure databases

    ProteinModelPortaliA0R220.
    SMRiA0R220. Positions 10-340.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni196 – 2005Pyridoxal phosphate bindingBy similarity

    Sequence similaritiesi

    Belongs to the threonine synthase family.Curated

    Phylogenomic databases

    eggNOGiCOG0498.
    HOGENOMiHOG000076503.
    KOiK01733.
    OMAiDPDWAVA.
    OrthoDBiEOG6HMX9M.

    Family and domain databases

    InterProiIPR000634. Ser/Thr_deHydtase_PyrdxlP-BS.
    IPR026260. Thr_Synthase_bac/arc.
    IPR004450. Thr_synthase_like.
    IPR001926. Trp_syn_b_sub_like_PLP_eny_SF.
    [Graphical view]
    PfamiPF00291. PALP. 1 hit.
    [Graphical view]
    PIRSFiPIRSF038945. Thr_synthase. 1 hit.
    SUPFAMiSSF53686. SSF53686. 1 hit.
    TIGRFAMsiTIGR00260. thrC. 1 hit.
    PROSITEiPS00165. DEHYDRATASE_SER_THR. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    A0R220-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSAAKAAVHQ PWPGLIEAYR DRLPIGDDWT TVTLLEGGTP LIHAKRISEL    50
    TGCTVHLKVE GLNPTGSFKD RGMTVAVTES LARGQQAVLC ASTGNTSASA 100
    AAYAARAGIT CAVLIPQGKI AMGKLAQAVM HGAKIIQVDG NFDDCLELAR 150
    KLTADFPTIA LVNSVNPYRI EGQKTAAFEI VDALGTAPDV HALPVGNAGN 200
    ITAYWKGYSE YHRDGVSDRL PRMLGTQAAG AAPLVTGAPV KDPETIATAI 250
    RIGSPASWNS AVEAQQQSDG RFLAATDEEI LAAYHLVART EGVFVEPASA 300
    ASIAGLLKSV EDGWVKRGST VVCTVTGNGL KDPDTALKGM PQVTPVPVDP 350
    SAVVAELGLS 360
    Length:360
    Mass (Da):37,443
    Last modified:January 9, 2007 - v1
    Checksum:iC40EF7EED839FE94
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000480 Genomic DNA. Translation: ABK71073.1.
    CP001663 Genomic DNA. Translation: AFP41274.1.
    RefSeqiYP_006569569.1. NC_018289.1.
    YP_889208.1. NC_008596.1.

    Genome annotation databases

    EnsemblBacteriaiABK71073; ABK71073; MSMEG_4956.
    AFP41274; AFP41274; MSMEI_4829.
    GeneIDi4536683.
    KEGGimsg:MSMEI_4829.
    msm:MSMEG_4956.
    PATRICi18082211. VBIMycSme59918_4835.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000480 Genomic DNA. Translation: ABK71073.1 .
    CP001663 Genomic DNA. Translation: AFP41274.1 .
    RefSeqi YP_006569569.1. NC_018289.1.
    YP_889208.1. NC_008596.1.

    3D structure databases

    ProteinModelPortali A0R220.
    SMRi A0R220. Positions 10-340.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 246196.MSMEG_4956.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai ABK71073 ; ABK71073 ; MSMEG_4956 .
    AFP41274 ; AFP41274 ; MSMEI_4829 .
    GeneIDi 4536683.
    KEGGi msg:MSMEI_4829.
    msm:MSMEG_4956.
    PATRICi 18082211. VBIMycSme59918_4835.

    Phylogenomic databases

    eggNOGi COG0498.
    HOGENOMi HOG000076503.
    KOi K01733.
    OMAi DPDWAVA.
    OrthoDBi EOG6HMX9M.

    Enzyme and pathway databases

    UniPathwayi UPA00050 ; UER00065 .
    BioCyci MSME246196:GJ4Y-4955-MONOMER.

    Family and domain databases

    InterProi IPR000634. Ser/Thr_deHydtase_PyrdxlP-BS.
    IPR026260. Thr_Synthase_bac/arc.
    IPR004450. Thr_synthase_like.
    IPR001926. Trp_syn_b_sub_like_PLP_eny_SF.
    [Graphical view ]
    Pfami PF00291. PALP. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF038945. Thr_synthase. 1 hit.
    SUPFAMi SSF53686. SSF53686. 1 hit.
    TIGRFAMsi TIGR00260. thrC. 1 hit.
    PROSITEi PS00165. DEHYDRATASE_SER_THR. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Fleischmann R.D., Dodson R.J., Haft D.H., Merkel J.S., Nelson W.C., Fraser C.M.
      Submitted (OCT-2006) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 700084 / mc(2)155.
    2. "Interrupted coding sequences in Mycobacterium smegmatis: authentic mutations or sequencing errors?"
      Deshayes C., Perrodou E., Gallien S., Euphrasie D., Schaeffer C., Van-Dorsselaer A., Poch O., Lecompte O., Reyrat J.-M.
      Genome Biol. 8:R20.1-R20.9(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 700084 / mc(2)155.
    3. "Ortho-proteogenomics: multiple proteomes investigation through orthology and a new MS-based protocol."
      Gallien S., Perrodou E., Carapito C., Deshayes C., Reyrat J.-M., Van Dorsselaer A., Poch O., Schaeffer C., Lecompte O.
      Genome Res. 19:128-135(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 700084 / mc(2)155.
    4. Cited for: PUPYLATION AT LYS-151, IDENTIFICATION BY MASS SPECTROMETRY.

    Entry informationi

    Entry nameiTHRC_MYCS2
    AccessioniPrimary (citable) accession number: A0R220
    Secondary accession number(s): I7GEF5
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: August 10, 2010
    Last sequence update: January 9, 2007
    Last modified: October 1, 2014
    This is version 57 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3