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Protein

Threonine synthase

Gene

thrC

Organism
Mycobacterium smegmatis (strain ATCC 700084 / mc(2)155)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the gamma-elimination of phosphate from L-phosphohomoserine and the beta-addition of water to produce L-threonine.By similarity

Catalytic activityi

O-phospho-L-homoserine + H2O = L-threonine + phosphate.

Cofactori

Pathway: L-threonine biosynthesis

This protein is involved in step 5 of the subpathway that synthesizes L-threonine from L-aspartate.
Proteins known to be involved in the 5 steps of the subpathway in this organism are:
  1. Aspartokinase (ask)
  2. Aspartate-semialdehyde dehydrogenase (asd)
  3. Homoserine dehydrogenase (MSMEG_4957)
  4. Homoserine kinase (thrB), Homoserine kinase (thrB)
  5. Threonine synthase (thrC), Threonine synthase (LJ00_24505)
This subpathway is part of the pathway L-threonine biosynthesis, which is itself part of Amino-acid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes L-threonine from L-aspartate, the pathway L-threonine biosynthesis and in Amino-acid biosynthesis.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei95 – 951Pyridoxal phosphateBy similarity
Binding sitei326 – 3261Pyridoxal phosphateBy similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Lyase

Keywords - Biological processi

Amino-acid biosynthesis, Threonine biosynthesis

Keywords - Ligandi

Pyridoxal phosphate

Enzyme and pathway databases

BioCyciMSME246196:GJ4Y-4955-MONOMER.
UniPathwayiUPA00050; UER00065.

Names & Taxonomyi

Protein namesi
Recommended name:
Threonine synthase (EC:4.2.3.1)
Short name:
TS
Gene namesi
Name:thrC
Ordered Locus Names:MSMEG_4956, MSMEI_4829
OrganismiMycobacterium smegmatis (strain ATCC 700084 / mc(2)155)
Taxonomic identifieri246196 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaCorynebacterialesMycobacteriaceaeMycobacterium
ProteomesiUP000006158 Componenti: Chromosome UP000000757 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 360360Threonine synthasePRO_0000396139Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei69 – 691N6-(pyridoxal phosphate)lysineBy similarity
Cross-linki151 – 151Isoglutamyl lysine isopeptide (Lys-Gln) (interchain with Q-Cter in protein Pup)1 Publication

Keywords - PTMi

Isopeptide bond, Ubl conjugation

Interactioni

Subunit structurei

Homodimer.By similarity

Protein-protein interaction databases

STRINGi246196.MSMEG_4956.

Structurei

3D structure databases

ProteinModelPortaliA0R220.
SMRiA0R220. Positions 10-340.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni196 – 2005Pyridoxal phosphate bindingBy similarity

Sequence similaritiesi

Belongs to the threonine synthase family.Curated

Phylogenomic databases

eggNOGiCOG0498.
HOGENOMiHOG000076503.
KOiK01733.
OMAiGMRAFVL.
OrthoDBiEOG6HMX9M.

Family and domain databases

InterProiIPR000634. Ser/Thr_deHydtase_PyrdxlP-BS.
IPR026260. Thr_Synthase_bac/arc.
IPR004450. Thr_synthase_like.
IPR001926. TrpB-like_PLP-dep.
[Graphical view]
PfamiPF00291. PALP. 1 hit.
[Graphical view]
PIRSFiPIRSF038945. Thr_synthase. 1 hit.
SUPFAMiSSF53686. SSF53686. 1 hit.
TIGRFAMsiTIGR00260. thrC. 1 hit.
PROSITEiPS00165. DEHYDRATASE_SER_THR. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

A0R220-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSAAKAAVHQ PWPGLIEAYR DRLPIGDDWT TVTLLEGGTP LIHAKRISEL
60 70 80 90 100
TGCTVHLKVE GLNPTGSFKD RGMTVAVTES LARGQQAVLC ASTGNTSASA
110 120 130 140 150
AAYAARAGIT CAVLIPQGKI AMGKLAQAVM HGAKIIQVDG NFDDCLELAR
160 170 180 190 200
KLTADFPTIA LVNSVNPYRI EGQKTAAFEI VDALGTAPDV HALPVGNAGN
210 220 230 240 250
ITAYWKGYSE YHRDGVSDRL PRMLGTQAAG AAPLVTGAPV KDPETIATAI
260 270 280 290 300
RIGSPASWNS AVEAQQQSDG RFLAATDEEI LAAYHLVART EGVFVEPASA
310 320 330 340 350
ASIAGLLKSV EDGWVKRGST VVCTVTGNGL KDPDTALKGM PQVTPVPVDP
360
SAVVAELGLS
Length:360
Mass (Da):37,443
Last modified:January 9, 2007 - v1
Checksum:iC40EF7EED839FE94
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000480 Genomic DNA. Translation: ABK71073.1.
CP001663 Genomic DNA. Translation: AFP41274.1.
RefSeqiWP_011730211.1. NC_018289.1.
YP_006569569.1. NC_018289.1.
YP_889208.1. NC_008596.1.

Genome annotation databases

EnsemblBacteriaiABK71073; ABK71073; MSMEG_4956.
AFP41274; AFP41274; MSMEI_4829.
GeneIDi4536683.
KEGGimsm:MSMEG_4956.
PATRICi18082211. VBIMycSme59918_4835.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000480 Genomic DNA. Translation: ABK71073.1.
CP001663 Genomic DNA. Translation: AFP41274.1.
RefSeqiWP_011730211.1. NC_018289.1.
YP_006569569.1. NC_018289.1.
YP_889208.1. NC_008596.1.

3D structure databases

ProteinModelPortaliA0R220.
SMRiA0R220. Positions 10-340.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi246196.MSMEG_4956.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABK71073; ABK71073; MSMEG_4956.
AFP41274; AFP41274; MSMEI_4829.
GeneIDi4536683.
KEGGimsm:MSMEG_4956.
PATRICi18082211. VBIMycSme59918_4835.

Phylogenomic databases

eggNOGiCOG0498.
HOGENOMiHOG000076503.
KOiK01733.
OMAiGMRAFVL.
OrthoDBiEOG6HMX9M.

Enzyme and pathway databases

UniPathwayiUPA00050; UER00065.
BioCyciMSME246196:GJ4Y-4955-MONOMER.

Family and domain databases

InterProiIPR000634. Ser/Thr_deHydtase_PyrdxlP-BS.
IPR026260. Thr_Synthase_bac/arc.
IPR004450. Thr_synthase_like.
IPR001926. TrpB-like_PLP-dep.
[Graphical view]
PfamiPF00291. PALP. 1 hit.
[Graphical view]
PIRSFiPIRSF038945. Thr_synthase. 1 hit.
SUPFAMiSSF53686. SSF53686. 1 hit.
TIGRFAMsiTIGR00260. thrC. 1 hit.
PROSITEiPS00165. DEHYDRATASE_SER_THR. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. Fleischmann R.D., Dodson R.J., Haft D.H., Merkel J.S., Nelson W.C., Fraser C.M.
    Submitted (OCT-2006) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 700084 / mc(2)155.
  2. "Interrupted coding sequences in Mycobacterium smegmatis: authentic mutations or sequencing errors?"
    Deshayes C., Perrodou E., Gallien S., Euphrasie D., Schaeffer C., Van-Dorsselaer A., Poch O., Lecompte O., Reyrat J.-M.
    Genome Biol. 8:R20.1-R20.9(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 700084 / mc(2)155.
  3. "Ortho-proteogenomics: multiple proteomes investigation through orthology and a new MS-based protocol."
    Gallien S., Perrodou E., Carapito C., Deshayes C., Reyrat J.-M., Van Dorsselaer A., Poch O., Schaeffer C., Lecompte O.
    Genome Res. 19:128-135(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 700084 / mc(2)155.
  4. Cited for: PUPYLATION AT LYS-151, IDENTIFICATION BY MASS SPECTROMETRY.

Entry informationi

Entry nameiTHRC_MYCS2
AccessioniPrimary (citable) accession number: A0R220
Secondary accession number(s): I7GEF5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 10, 2010
Last sequence update: January 9, 2007
Last modified: June 24, 2015
This is version 63 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.