A0R219 (KHSE_MYCS2) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Homoserine kinase
Short name=HK
Short name=HSK
EC=2.7.1.39

Gene names

Name:	thrB
Ordered Locus Names:	MSMEG_4955

Organism

Mycobacterium smegmatis (strain ATCC 700084 / mc(2)155)

Taxonomic identifier

246196 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Corynebacterineae › Mycobacteriaceae › Mycobacterium

Protein attributes

Sequence length	317 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the ATP-dependent phosphorylation of L-homoserine to L-homoserine phosphate By similarity. HAMAP MF_00384
Catalytic activity	ATP + L-homoserine = ADP + O-phospho-L-homoserine. HAMAP MF_00384
Pathway	Amino-acid biosynthesis; L-threonine biosynthesis; L-threonine from L-aspartate: step 4/5. HAMAP MF_00384
Subcellular location	Cytoplasm Potential HAMAP MF_00384.
Sequence similarities	Belongs to the GHMP kinase family. Homoserine kinase subfamily.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Threonine biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	threonine biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW homoserine kinase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 317	317	Homoserine kinase HAMAP MF_00384		PRO_1000049147
Regions
	Nucleotide binding	95 – 105	11	ATP Potential

Sequences

	Sequence		Length	Mass (Da)
	A0R219 [UniParc]. Last modified January 9, 2007. Version 1. Checksum: 3B78DE84F5C0DA98	FASTA	317	32,443
	10 20 30 40 50 60 MTQTLPVGLS ATSVVAASSA NLGPGFDSLG IALSLYDEIV VETVESGLTV HVEGEGAGQV 70 80 90 100 110 120 PLDQTHLVVR AINKGLQAAG FSAPGLVVRC RNDIPHSRGL GSSAAAVVGG LAAVNGLVTQ 130 140 150 160 170 180 AGSEPMDDAR LIQLSSEFEG HPDNAAAAVL GGAVVSWTEQ AGAFGGEPRY SAVPVRLHPD 190 200 210 220 230 240 IRLFPAVPQQ RSSTAETRVL LPEQVSHVDA RFNLSRAALL VVALSERPDL LMAATEDVLH 250 260 270 280 290 300 QPQRAAAMPA SAEYLAILRR CGIAAVLSGA GPSVLGLSAQ SGLPAEALEY GTAHGFTVTE 310 MSVGAGVRWT AGAVVRN

References

[1]	Fleischmann R.D., Dodson R.J., Haft D.H., Merkel J.S., Nelson W.C., Fraser C.M. Submitted (OCT-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 700084 / mc(2)155.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000480 Genomic DNA. Translation: ABK72678.1.
RefSeq	YP_889207.1. NC_008596.1.
3D structure databases
ProteinModelPortal	A0R219.
ModBase	Search...
Protein-protein interaction databases
STRING	A0R219.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBMYCT00000042223; EBMYCP00000040560; EBMYCG00000042218.
GeneID	4537179.
GenomeReviews	Gene locus MSMEG_4955 in contig CP000480_GR.
KEGG	msm:MSMEG_4955.
NMPDR	fig|246196.1.peg.4941.
PATRIC	18082209. VBIMycSme59918_4834.
TIGR	MSMEG_4955.
Phylogenomic databases
eggNOG	COG0083.
GeneTree	EBGT00050000016145.
HOGENOM	HBG646290.
OMA	DRLHQPY.
PhylomeDB	A0R219.
ProtClustDB	PRK01212.
Enzyme and pathway databases
BioCyc	MSME246196:MSMEG_4955-MONOMER.
Family and domain databases
HAMAP	MF_00384. Homoser_kinase. [Tree]
InterPro	IPR006204. GHMP_kinase. IPR013750. GHMP_kinase_C. IPR006203. GHMP_knse_ATP-bd_CS. IPR000870. Homoserine_kinase. IPR020568. Ribosomal_S5_D2-typ_fold. IPR014721. Ribosomal_S5_D2-typ_fold_subgr. [Graphical view]
Gene3D	G3DSA:3.30.230.10. Ribosomal_S5_D2-type_fold. 1 hit.
KO	K00872.
Pfam	PF08544. GHMP_kinases_C. 1 hit. PF00288. GHMP_kinases_N. 1 hit. [Graphical view]
PIRSF	PIRSF000676. Homoser_kin. 1 hit.
PRINTS	PR00958. HOMSERKINASE.
SUPFAM	SSF54211. Ribosomal_S5_D2-typ_fold. 1 hit.
TIGRFAMs	TIGR00191. ThrB. 1 hit.
PROSITE	PS00627. GHMP_KINASES_ATP. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

KHSE_MYCS2

Accession

Primary (citable) accession number: A0R219

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	January 9, 2007
Last modified:	December 14, 2011
This is version 41 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program