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Protein

1,4-alpha-glucan branching enzyme GlgB

Gene

glgB

Organism
Mycobacterium smegmatis (strain ATCC 700084 / mc(2)155)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the formation of the alpha-1,6-glucosidic linkages in glycogen by scission of a 1,4-alpha-linked oligosaccharide from growing alpha-1,4-glucan chains and the subsequent attachment of the oligosaccharide to the alpha-1,6 position.By similarity

Catalytic activityi

Transfers a segment of a (1->4)-alpha-D-glucan chain to a primary hydroxy group in a similar glucan chain.

Pathway:iglycogen biosynthesis

This protein is involved in the pathway glycogen biosynthesis, which is part of Glycan biosynthesis.
View all proteins of this organism that are known to be involved in the pathway glycogen biosynthesis and in Glycan biosynthesis.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei416 – 4161NucleophileBy similarity
Active sitei469 – 4691Proton donorBy similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Transferase

Keywords - Biological processi

Carbohydrate metabolism, Glycogen biosynthesis, Glycogen metabolism

Enzyme and pathway databases

BioCyciMSME246196:GJ4Y-4916-MONOMER.
UniPathwayiUPA00164.

Protein family/group databases

CAZyiCBM48. Carbohydrate-Binding Module Family 48.
GH13. Glycoside Hydrolase Family 13.

Names & Taxonomyi

Protein namesi
Recommended name:
1,4-alpha-glucan branching enzyme GlgB (EC:2.4.1.18)
Alternative name(s):
1,4-alpha-D-glucan:1,4-alpha-D-glucan 6-glucosyl-transferase
Glycogen-branching enzyme
Short name:
BE
Gene namesi
Name:glgB
Ordered Locus Names:MSMEG_4918, MSMEI_4790
OrganismiMycobacterium smegmatis (strain ATCC 700084 / mc(2)155)
Taxonomic identifieri246196 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaCorynebacterialesMycobacteriaceaeMycobacterium
ProteomesiUP000006158 Componenti: Chromosome UP000000757 Componenti: Chromosome

Pathology & Biotechi

Disruption phenotypei

Cells lacking this gene display maltose 1-phosphate (M1P) accumulation and trehalose sensitivity. These phenotypes are suppressed in mutants lacking both glgE and treS or both glgE and mak.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 7367361,4-alpha-glucan branching enzyme GlgBPRO_0000413977Add
BLAST

Interactioni

Subunit structurei

Monomer.By similarity

Protein-protein interaction databases

STRINGi246196.MSMEG_4918.

Structurei

3D structure databases

ProteinModelPortaliA0R1Y4.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0296.
HOGENOMiHOG000283037.
KOiK00700.
OMAiDDADHNT.
OrthoDBiEOG6JX7GT.

Family and domain databases

Gene3Di2.60.40.10. 2 hits.
2.60.40.1180. 1 hit.
3.20.20.80. 1 hit.
HAMAPiMF_00685. GlgB.
InterProiIPR006048. A-amylase_b_C.
IPR006407. GlgB.
IPR015902. Glyco_hydro_13.
IPR013780. Glyco_hydro_13_b.
IPR006047. Glyco_hydro_13_cat_dom.
IPR004193. Glyco_hydro_13_N.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
IPR013783. Ig-like_fold.
IPR014756. Ig_E-set.
[Graphical view]
PANTHERiPTHR10357. PTHR10357. 1 hit.
PfamiPF00128. Alpha-amylase. 1 hit.
PF02806. Alpha-amylase_C. 1 hit.
PF02922. CBM_48. 1 hit.
[Graphical view]
PIRSFiPIRSF000463. GlgB. 1 hit.
SUPFAMiSSF51445. SSF51445. 1 hit.
SSF81296. SSF81296. 2 hits.
TIGRFAMsiTIGR01515. branching_enzym. 1 hit.

Sequencei

Sequence statusi: Complete.

A0R1Y4-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTRSSNQLTD AHLRPDPSDI HRLLAGEHHD PHSVLGAHEY PGHTVIRAFR
60 70 80 90 100
PHAVKVTAVI GGERHVMQHL ESGLFAVAVP FTNLIDYRLE IDYPGVGDAV
110 120 130 140 150
VTHTTADAYR FLPTLGELDL HLFAEGRHER LWEILGAHRR TFTTADGPVS
160 170 180 190 200
GVSFAVWAPN AKGVSLIGEF NHWGGNDAPM RVLGSSGVWE LFWPGFPPDG
210 220 230 240 250
LYKFRVHGAD GSVTDRADPM AFATEVPPHT ASRVTESTYE WHDEDWMTRR
260 270 280 290 300
ASLNPVFEPM STLEVHLLSW RPGLTYRQLA TELTEYVVEQ GFTHVEMLPV
310 320 330 340 350
AEHPFGGSWG YQVTSYYAPT SRLGTPDDFR YLVDALHQAG IGVIVDWVPA
360 370 380 390 400
HFPKDAWALG RFDGTALYEH SDPRRGEQLD WGTYVFDFGR PEVRNFLVAN
410 420 430 440 450
ALYWLQEFHI DGLRVDAVAS MLYLDYSRPE GGWTPNQYGG RENLEAVQFL
460 470 480 490 500
QEMNATVHKV APGIVTVAEE STSWPGVTRP TNLGGLGFSM KWNMGWMNDT
510 520 530 540 550
LAYISRDPIY RSYHHHEMTF SMLYAYSENY VLPISHDEVV HGKGTLWSRM
560 570 580 590 600
PGGDHEKAAG LRSLLAYQWA HPGKQLLFMG QEFGQRAEWS EERGLDWYQL
610 620 630 640 650
DEQSFSTGVQ NLVRDLNAIY RSRPALWSRD TSPEGYSWID ANDSANNVLS
660 670 680 690 700
FLRFGNDGSM LACVINFAGS EHSQYRLGLP HAGTWREVLN TDADIYNGSG
710 720 730
IGNYGAVEAT DEPWHGRPAS AVMVLPPLSM LWFEPA
Length:736
Mass (Da):82,656
Last modified:January 9, 2007 - v1
Checksum:i937C85D1A22A6A73
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000480 Genomic DNA. Translation: ABK70471.1.
CP001663 Genomic DNA. Translation: AFP41238.1.
RefSeqiWP_011730189.1. NZ_CP009494.1.
YP_889172.1. NC_008596.1.

Genome annotation databases

EnsemblBacteriaiABK70471; ABK70471; MSMEG_4918.
AFP41238; AFP41238; MSMEI_4790.
GeneIDi4535775.
KEGGimsg:MSMEI_4790.
msm:MSMEG_4918.
PATRICi18082131. VBIMycSme59918_4798.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000480 Genomic DNA. Translation: ABK70471.1.
CP001663 Genomic DNA. Translation: AFP41238.1.
RefSeqiWP_011730189.1. NZ_CP009494.1.
YP_889172.1. NC_008596.1.

3D structure databases

ProteinModelPortaliA0R1Y4.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi246196.MSMEG_4918.

Protein family/group databases

CAZyiCBM48. Carbohydrate-Binding Module Family 48.
GH13. Glycoside Hydrolase Family 13.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABK70471; ABK70471; MSMEG_4918.
AFP41238; AFP41238; MSMEI_4790.
GeneIDi4535775.
KEGGimsg:MSMEI_4790.
msm:MSMEG_4918.
PATRICi18082131. VBIMycSme59918_4798.

Phylogenomic databases

eggNOGiCOG0296.
HOGENOMiHOG000283037.
KOiK00700.
OMAiDDADHNT.
OrthoDBiEOG6JX7GT.

Enzyme and pathway databases

UniPathwayiUPA00164.
BioCyciMSME246196:GJ4Y-4916-MONOMER.

Family and domain databases

Gene3Di2.60.40.10. 2 hits.
2.60.40.1180. 1 hit.
3.20.20.80. 1 hit.
HAMAPiMF_00685. GlgB.
InterProiIPR006048. A-amylase_b_C.
IPR006407. GlgB.
IPR015902. Glyco_hydro_13.
IPR013780. Glyco_hydro_13_b.
IPR006047. Glyco_hydro_13_cat_dom.
IPR004193. Glyco_hydro_13_N.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
IPR013783. Ig-like_fold.
IPR014756. Ig_E-set.
[Graphical view]
PANTHERiPTHR10357. PTHR10357. 1 hit.
PfamiPF00128. Alpha-amylase. 1 hit.
PF02806. Alpha-amylase_C. 1 hit.
PF02922. CBM_48. 1 hit.
[Graphical view]
PIRSFiPIRSF000463. GlgB. 1 hit.
SUPFAMiSSF51445. SSF51445. 1 hit.
SSF81296. SSF81296. 2 hits.
TIGRFAMsiTIGR01515. branching_enzym. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. Fleischmann R.D., Dodson R.J., Haft D.H., Merkel J.S., Nelson W.C., Fraser C.M.
    Submitted (OCT-2006) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 700084 / mc(2)155.
  2. "Interrupted coding sequences in Mycobacterium smegmatis: authentic mutations or sequencing errors?"
    Deshayes C., Perrodou E., Gallien S., Euphrasie D., Schaeffer C., Van-Dorsselaer A., Poch O., Lecompte O., Reyrat J.-M.
    Genome Biol. 8:R20.1-R20.9(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 700084 / mc(2)155.
  3. "Ortho-proteogenomics: multiple proteomes investigation through orthology and a new MS-based protocol."
    Gallien S., Perrodou E., Carapito C., Deshayes C., Reyrat J.-M., Van Dorsselaer A., Poch O., Schaeffer C., Lecompte O.
    Genome Res. 19:128-135(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 700084 / mc(2)155.
  4. "Self-poisoning of Mycobacterium tuberculosis by targeting GlgE in an alpha-glucan pathway."
    Kalscheuer R., Syson K., Veeraraghavan U., Weinrick B., Biermann K.E., Liu Z., Sacchettini J.C., Besra G., Bornemann S., Jacobs W.R. Jr.
    Nat. Chem. Biol. 6:376-384(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: DISRUPTION PHENOTYPE.
    Strain: ATCC 700084 / mc(2)155.

Entry informationi

Entry nameiGLGB_MYCS2
AccessioniPrimary (citable) accession number: A0R1Y4
Secondary accession number(s): I7FR43
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 16, 2011
Last sequence update: January 9, 2007
Last modified: July 22, 2015
This is version 78 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.