Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

A0R1Y4

- GLGB_MYCS2

UniProt

A0R1Y4 - GLGB_MYCS2

Protein

1,4-alpha-glucan branching enzyme GlgB

Gene

glgB

Organism
Mycobacterium smegmatis (strain ATCC 700084 / mc(2)155)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
    • BLAST
    • Align
    • Format
    • Add to basket
    • History
      Entry version 71 (01 Oct 2014)
      Sequence version 1 (09 Jan 2007)
      Previous versions | rss
    • Help video
    • Feedback
    • Comment

    Functioni

    Catalyzes the formation of the alpha-1,6-glucosidic linkages in glycogen by scission of a 1,4-alpha-linked oligosaccharide from growing alpha-1,4-glucan chains and the subsequent attachment of the oligosaccharide to the alpha-1,6 position.By similarity

    Catalytic activityi

    Transfers a segment of a (1->4)-alpha-D-glucan chain to a primary hydroxy group in a similar glucan chain.

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei416 – 4161NucleophileBy similarity
    Active sitei469 – 4691Proton donorBy similarity

    GO - Molecular functioni

    1. 1,4-alpha-glucan branching enzyme activity Source: UniProtKB-HAMAP
    2. cation binding Source: InterPro
    3. hydrolase activity, hydrolyzing O-glycosyl compounds Source: InterPro

    GO - Biological processi

    1. glycogen biosynthetic process Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Glycosyltransferase, Transferase

    Keywords - Biological processi

    Carbohydrate metabolism, Glycogen biosynthesis, Glycogen metabolism

    Enzyme and pathway databases

    BioCyciMSME246196:GJ4Y-4916-MONOMER.
    UniPathwayiUPA00164.

    Protein family/group databases

    CAZyiCBM48. Carbohydrate-Binding Module Family 48.
    GH13. Glycoside Hydrolase Family 13.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    1,4-alpha-glucan branching enzyme GlgB (EC:2.4.1.18)
    Alternative name(s):
    1,4-alpha-D-glucan:1,4-alpha-D-glucan 6-glucosyl-transferase
    Glycogen-branching enzyme
    Short name:
    BE
    Gene namesi
    Name:glgB
    Ordered Locus Names:MSMEG_4918, MSMEI_4790
    OrganismiMycobacterium smegmatis (strain ATCC 700084 / mc(2)155)
    Taxonomic identifieri246196 [NCBI]
    Taxonomic lineageiBacteriaActinobacteriaActinobacteridaeActinomycetalesCorynebacterineaeMycobacteriaceaeMycobacterium
    ProteomesiUP000000757: Chromosome, UP000006158: Chromosome

    Pathology & Biotechi

    Disruption phenotypei

    Cells lacking this gene display maltose 1-phosphate (M1P) accumulation and trehalose sensitivity. These phenotypes are suppressed in mutants lacking both glgE and treS or both glgE and mak.1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 7367361,4-alpha-glucan branching enzyme GlgBPRO_0000413977Add
    BLAST

    Interactioni

    Subunit structurei

    Monomer.By similarity

    Protein-protein interaction databases

    STRINGi246196.MSMEG_4918.

    Structurei

    3D structure databases

    ProteinModelPortaliA0R1Y4.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Phylogenomic databases

    eggNOGiCOG0296.
    HOGENOMiHOG000283037.
    KOiK00700.
    OMAiISEGRHE.
    OrthoDBiEOG6JX7GT.

    Family and domain databases

    Gene3Di2.60.40.10. 2 hits.
    2.60.40.1180. 1 hit.
    3.20.20.80. 1 hit.
    HAMAPiMF_00685. GlgB.
    InterProiIPR006048. A-amylase_b_C.
    IPR006407. GlgB.
    IPR015902. Glyco_hydro_13.
    IPR013780. Glyco_hydro_13_b.
    IPR006047. Glyco_hydro_13_cat_dom.
    IPR004193. Glyco_hydro_13_N.
    IPR013781. Glyco_hydro_catalytic_dom.
    IPR017853. Glycoside_hydrolase_SF.
    IPR013783. Ig-like_fold.
    IPR014756. Ig_E-set.
    [Graphical view]
    PANTHERiPTHR10357. PTHR10357. 1 hit.
    PfamiPF00128. Alpha-amylase. 1 hit.
    PF02806. Alpha-amylase_C. 1 hit.
    PF02922. CBM_48. 1 hit.
    [Graphical view]
    PIRSFiPIRSF000463. GlgB. 1 hit.
    SUPFAMiSSF51445. SSF51445. 1 hit.
    SSF81296. SSF81296. 2 hits.
    TIGRFAMsiTIGR01515. branching_enzym. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    A0R1Y4-1 [UniParc]FASTAAdd to Basket

    « Hide

    MTRSSNQLTD AHLRPDPSDI HRLLAGEHHD PHSVLGAHEY PGHTVIRAFR    50
    PHAVKVTAVI GGERHVMQHL ESGLFAVAVP FTNLIDYRLE IDYPGVGDAV 100
    VTHTTADAYR FLPTLGELDL HLFAEGRHER LWEILGAHRR TFTTADGPVS 150
    GVSFAVWAPN AKGVSLIGEF NHWGGNDAPM RVLGSSGVWE LFWPGFPPDG 200
    LYKFRVHGAD GSVTDRADPM AFATEVPPHT ASRVTESTYE WHDEDWMTRR 250
    ASLNPVFEPM STLEVHLLSW RPGLTYRQLA TELTEYVVEQ GFTHVEMLPV 300
    AEHPFGGSWG YQVTSYYAPT SRLGTPDDFR YLVDALHQAG IGVIVDWVPA 350
    HFPKDAWALG RFDGTALYEH SDPRRGEQLD WGTYVFDFGR PEVRNFLVAN 400
    ALYWLQEFHI DGLRVDAVAS MLYLDYSRPE GGWTPNQYGG RENLEAVQFL 450
    QEMNATVHKV APGIVTVAEE STSWPGVTRP TNLGGLGFSM KWNMGWMNDT 500
    LAYISRDPIY RSYHHHEMTF SMLYAYSENY VLPISHDEVV HGKGTLWSRM 550
    PGGDHEKAAG LRSLLAYQWA HPGKQLLFMG QEFGQRAEWS EERGLDWYQL 600
    DEQSFSTGVQ NLVRDLNAIY RSRPALWSRD TSPEGYSWID ANDSANNVLS 650
    FLRFGNDGSM LACVINFAGS EHSQYRLGLP HAGTWREVLN TDADIYNGSG 700
    IGNYGAVEAT DEPWHGRPAS AVMVLPPLSM LWFEPA 736
    Length:736
    Mass (Da):82,656
    Last modified:January 9, 2007 - v1
    Checksum:i937C85D1A22A6A73
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000480 Genomic DNA. Translation: ABK70471.1.
    CP001663 Genomic DNA. Translation: AFP41238.1.
    RefSeqiWP_011730189.1. NC_018289.1.
    YP_006569533.1. NC_018289.1.
    YP_889172.1. NC_008596.1.

    Genome annotation databases

    EnsemblBacteriaiABK70471; ABK70471; MSMEG_4918.
    AFP41238; AFP41238; MSMEI_4790.
    GeneIDi4535775.
    KEGGimsg:MSMEI_4790.
    msm:MSMEG_4918.
    PATRICi18082131. VBIMycSme59918_4798.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000480 Genomic DNA. Translation: ABK70471.1 .
    CP001663 Genomic DNA. Translation: AFP41238.1 .
    RefSeqi WP_011730189.1. NC_018289.1.
    YP_006569533.1. NC_018289.1.
    YP_889172.1. NC_008596.1.

    3D structure databases

    ProteinModelPortali A0R1Y4.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 246196.MSMEG_4918.

    Protein family/group databases

    CAZyi CBM48. Carbohydrate-Binding Module Family 48.
    GH13. Glycoside Hydrolase Family 13.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai ABK70471 ; ABK70471 ; MSMEG_4918 .
    AFP41238 ; AFP41238 ; MSMEI_4790 .
    GeneIDi 4535775.
    KEGGi msg:MSMEI_4790.
    msm:MSMEG_4918.
    PATRICi 18082131. VBIMycSme59918_4798.

    Phylogenomic databases

    eggNOGi COG0296.
    HOGENOMi HOG000283037.
    KOi K00700.
    OMAi ISEGRHE.
    OrthoDBi EOG6JX7GT.

    Enzyme and pathway databases

    UniPathwayi UPA00164 .
    BioCyci MSME246196:GJ4Y-4916-MONOMER.

    Family and domain databases

    Gene3Di 2.60.40.10. 2 hits.
    2.60.40.1180. 1 hit.
    3.20.20.80. 1 hit.
    HAMAPi MF_00685. GlgB.
    InterProi IPR006048. A-amylase_b_C.
    IPR006407. GlgB.
    IPR015902. Glyco_hydro_13.
    IPR013780. Glyco_hydro_13_b.
    IPR006047. Glyco_hydro_13_cat_dom.
    IPR004193. Glyco_hydro_13_N.
    IPR013781. Glyco_hydro_catalytic_dom.
    IPR017853. Glycoside_hydrolase_SF.
    IPR013783. Ig-like_fold.
    IPR014756. Ig_E-set.
    [Graphical view ]
    PANTHERi PTHR10357. PTHR10357. 1 hit.
    Pfami PF00128. Alpha-amylase. 1 hit.
    PF02806. Alpha-amylase_C. 1 hit.
    PF02922. CBM_48. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF000463. GlgB. 1 hit.
    SUPFAMi SSF51445. SSF51445. 1 hit.
    SSF81296. SSF81296. 2 hits.
    TIGRFAMsi TIGR01515. branching_enzym. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. Fleischmann R.D., Dodson R.J., Haft D.H., Merkel J.S., Nelson W.C., Fraser C.M.
      Submitted (OCT-2006) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 700084 / mc(2)155.
    2. "Interrupted coding sequences in Mycobacterium smegmatis: authentic mutations or sequencing errors?"
      Deshayes C., Perrodou E., Gallien S., Euphrasie D., Schaeffer C., Van-Dorsselaer A., Poch O., Lecompte O., Reyrat J.-M.
      Genome Biol. 8:R20.1-R20.9(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 700084 / mc(2)155.
    3. "Ortho-proteogenomics: multiple proteomes investigation through orthology and a new MS-based protocol."
      Gallien S., Perrodou E., Carapito C., Deshayes C., Reyrat J.-M., Van Dorsselaer A., Poch O., Schaeffer C., Lecompte O.
      Genome Res. 19:128-135(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 700084 / mc(2)155.
    4. "Self-poisoning of Mycobacterium tuberculosis by targeting GlgE in an alpha-glucan pathway."
      Kalscheuer R., Syson K., Veeraraghavan U., Weinrick B., Biermann K.E., Liu Z., Sacchettini J.C., Besra G., Bornemann S., Jacobs W.R. Jr.
      Nat. Chem. Biol. 6:376-384(2010) [PubMed] [Europe PMC] [Abstract]
      Cited for: DISRUPTION PHENOTYPE.
      Strain: ATCC 700084 / mc(2)155.

    Entry informationi

    Entry nameiGLGB_MYCS2
    AccessioniPrimary (citable) accession number: A0R1Y4
    Secondary accession number(s): I7FR43
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 16, 2011
    Last sequence update: January 9, 2007
    Last modified: October 1, 2014
    This is version 71 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Glycosyl hydrolases
      Classification of glycosyl hydrolase families and list of entries
    2. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3