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A0R1Y4 (GLGB_MYCS2) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 69. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
1,4-alpha-glucan branching enzyme GlgB

EC=2.4.1.18
Alternative name(s):
1,4-alpha-D-glucan:1,4-alpha-D-glucan 6-glucosyl-transferase
Glycogen-branching enzyme
Short name=BE
Gene names
Name:glgB
Ordered Locus Names:MSMEG_4918, MSMEI_4790
OrganismMycobacterium smegmatis (strain ATCC 700084 / mc(2)155) [Reference proteome] [HAMAP]
Taxonomic identifier246196 [NCBI]
Taxonomic lineageBacteriaActinobacteriaActinobacteridaeActinomycetalesCorynebacterineaeMycobacteriaceaeMycobacterium

Protein attributes

Sequence length736 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the formation of the alpha-1,6-glucosidic linkages in glycogen by scission of a 1,4-alpha-linked oligosaccharide from growing alpha-1,4-glucan chains and the subsequent attachment of the oligosaccharide to the alpha-1,6 position By similarity. HAMAP-Rule MF_00685

Catalytic activity

Transfers a segment of a (1->4)-alpha-D-glucan chain to a primary hydroxy group in a similar glucan chain. HAMAP-Rule MF_00685

Pathway

Glycan biosynthesis; glycogen biosynthesis. HAMAP-Rule MF_00685

Subunit structure

Monomer By similarity. HAMAP-Rule MF_00685

Disruption phenotype

Cells lacking this gene display maltose 1-phosphate (M1P) accumulation and trehalose sensitivity. These phenotypes are suppressed in mutants lacking both glgE and treS or both glgE and mak. Ref.4

Sequence similarities

Belongs to the glycosyl hydrolase 13 family. GlgB subfamily.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 7367361,4-alpha-glucan branching enzyme GlgB HAMAP-Rule MF_00685
PRO_0000413977

Sites

Active site4161Nucleophile By similarity
Active site4691Proton donor By similarity

Sequences

Sequence LengthMass (Da)Tools
A0R1Y4 [UniParc].

Last modified January 9, 2007. Version 1.
Checksum: 937C85D1A22A6A73

FASTA73682,656
        10         20         30         40         50         60 
MTRSSNQLTD AHLRPDPSDI HRLLAGEHHD PHSVLGAHEY PGHTVIRAFR PHAVKVTAVI 

        70         80         90        100        110        120 
GGERHVMQHL ESGLFAVAVP FTNLIDYRLE IDYPGVGDAV VTHTTADAYR FLPTLGELDL 

       130        140        150        160        170        180 
HLFAEGRHER LWEILGAHRR TFTTADGPVS GVSFAVWAPN AKGVSLIGEF NHWGGNDAPM 

       190        200        210        220        230        240 
RVLGSSGVWE LFWPGFPPDG LYKFRVHGAD GSVTDRADPM AFATEVPPHT ASRVTESTYE 

       250        260        270        280        290        300 
WHDEDWMTRR ASLNPVFEPM STLEVHLLSW RPGLTYRQLA TELTEYVVEQ GFTHVEMLPV 

       310        320        330        340        350        360 
AEHPFGGSWG YQVTSYYAPT SRLGTPDDFR YLVDALHQAG IGVIVDWVPA HFPKDAWALG 

       370        380        390        400        410        420 
RFDGTALYEH SDPRRGEQLD WGTYVFDFGR PEVRNFLVAN ALYWLQEFHI DGLRVDAVAS 

       430        440        450        460        470        480 
MLYLDYSRPE GGWTPNQYGG RENLEAVQFL QEMNATVHKV APGIVTVAEE STSWPGVTRP 

       490        500        510        520        530        540 
TNLGGLGFSM KWNMGWMNDT LAYISRDPIY RSYHHHEMTF SMLYAYSENY VLPISHDEVV 

       550        560        570        580        590        600 
HGKGTLWSRM PGGDHEKAAG LRSLLAYQWA HPGKQLLFMG QEFGQRAEWS EERGLDWYQL 

       610        620        630        640        650        660 
DEQSFSTGVQ NLVRDLNAIY RSRPALWSRD TSPEGYSWID ANDSANNVLS FLRFGNDGSM 

       670        680        690        700        710        720 
LACVINFAGS EHSQYRLGLP HAGTWREVLN TDADIYNGSG IGNYGAVEAT DEPWHGRPAS 

       730 
AVMVLPPLSM LWFEPA 

« Hide

References

« Hide 'large scale' references
[1]Fleischmann R.D., Dodson R.J., Haft D.H., Merkel J.S., Nelson W.C., Fraser C.M.
Submitted (OCT-2006) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 700084 / mc(2)155.
[2]"Interrupted coding sequences in Mycobacterium smegmatis: authentic mutations or sequencing errors?"
Deshayes C., Perrodou E., Gallien S., Euphrasie D., Schaeffer C., Van-Dorsselaer A., Poch O., Lecompte O., Reyrat J.-M.
Genome Biol. 8:R20.1-R20.9(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 700084 / mc(2)155.
[3]"Ortho-proteogenomics: multiple proteomes investigation through orthology and a new MS-based protocol."
Gallien S., Perrodou E., Carapito C., Deshayes C., Reyrat J.-M., Van Dorsselaer A., Poch O., Schaeffer C., Lecompte O.
Genome Res. 19:128-135(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 700084 / mc(2)155.
[4]"Self-poisoning of Mycobacterium tuberculosis by targeting GlgE in an alpha-glucan pathway."
Kalscheuer R., Syson K., Veeraraghavan U., Weinrick B., Biermann K.E., Liu Z., Sacchettini J.C., Besra G., Bornemann S., Jacobs W.R. Jr.
Nat. Chem. Biol. 6:376-384(2010) [PubMed] [Europe PMC] [Abstract]
Cited for: DISRUPTION PHENOTYPE.
Strain: ATCC 700084 / mc(2)155.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP000480 Genomic DNA. Translation: ABK70471.1.
CP001663 Genomic DNA. Translation: AFP41238.1.
RefSeqYP_006569533.1. NC_018289.1.
YP_889172.1. NC_008596.1.

3D structure databases

ProteinModelPortalA0R1Y4.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING246196.MSMEG_4918.

Protein family/group databases

CAZyCBM48. Carbohydrate-Binding Module Family 48.
GH13. Glycoside Hydrolase Family 13.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaABK70471; ABK70471; MSMEG_4918.
AFP41238; AFP41238; MSMEI_4790.
GeneID4535775.
KEGGmsg:MSMEI_4790.
msm:MSMEG_4918.
PATRIC18082131. VBIMycSme59918_4798.

Phylogenomic databases

eggNOGCOG0296.
HOGENOMHOG000283037.
KOK00700.
OMAISEGRHE.
OrthoDBEOG6JX7GT.

Enzyme and pathway databases

BioCycMSME246196:GJ4Y-4916-MONOMER.
UniPathwayUPA00164.

Family and domain databases

Gene3D2.60.40.10. 2 hits.
2.60.40.1180. 1 hit.
3.20.20.80. 1 hit.
HAMAPMF_00685. GlgB.
InterProIPR006048. A-amylase_b_C.
IPR006407. GlgB.
IPR015902. Glyco_hydro_13.
IPR013780. Glyco_hydro_13_b.
IPR006047. Glyco_hydro_13_cat_dom.
IPR004193. Glyco_hydro_13_N.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
IPR013783. Ig-like_fold.
IPR014756. Ig_E-set.
[Graphical view]
PANTHERPTHR10357. PTHR10357. 1 hit.
PfamPF00128. Alpha-amylase. 1 hit.
PF02806. Alpha-amylase_C. 1 hit.
PF02922. CBM_48. 1 hit.
[Graphical view]
PIRSFPIRSF000463. GlgB. 1 hit.
SUPFAMSSF51445. SSF51445. 1 hit.
SSF81296. SSF81296. 2 hits.
TIGRFAMsTIGR01515. branching_enzym. 1 hit.
ProtoNetSearch...

Entry information

Entry nameGLGB_MYCS2
AccessionPrimary (citable) accession number: A0R1Y4
Secondary accession number(s): I7FR43
Entry history
Integrated into UniProtKB/Swiss-Prot: November 16, 2011
Last sequence update: January 9, 2007
Last modified: July 9, 2014
This is version 69 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways

Glycosyl hydrolases

Classification of glycosyl hydrolase families and list of entries