A0R1W8 (RNPH_MYCS2) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Ribonuclease PH
Short name=RNase PH
EC=2.7.7.56

Alternative name(s):
tRNA nucleotidyltransferase

Gene names

Name:	rph
Ordered Locus Names:	MSMEG_4901

Organism

Mycobacterium smegmatis (strain ATCC 700084 / mc(2)155)

Taxonomic identifier

246196 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Corynebacterineae › Mycobacteriaceae › Mycobacterium

Protein attributes

Sequence length	259 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Phosphorolytic exoribonuclease that removes nucleotide residues following the -CCA terminus of tRNA and adds nucleotides to the ends of RNA molecules by using nucleoside diphosphates as substrates By similarity. HAMAP MF_00564
Catalytic activity	tRNA(n+1) + phosphate = tRNA(n) + a nucleoside diphosphate. HAMAP MF_00564
Sequence similarities	Belongs to the RNase PH family.

Ontologies

Keywords
Biological process	tRNA processing
Molecular function	Nucleotidyltransferase Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	tRNA processing Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	tRNA binding Inferred from electronic annotation. Source: InterPro tRNA nucleotidyltransferase activity Inferred from electronic annotation. Source: EC tRNA-specific ribonuclease activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 259	259	Ribonuclease PH HAMAP MF_00564		PRO_1000024830

Sequences

	Sequence		Length	Mass (Da)
	A0R1W8 [UniParc]. Last modified January 9, 2007. Version 1. Checksum: 0B705F2B90061CD2	FASTA	259	27,384
	10 20 30 40 50 60 MSRREDGRLD DELRPVVITR GFTSHPAGSV LVEFGQTRVM CTASVTEGVP RWRKGSGQGW 70 80 90 100 110 120 LTAEYAMLPA ATHDRSDRES VKGRIGGRTQ EISRLIGRSL RACIDLAALG ENTIAIDCDV 130 140 150 160 170 180 LQADGGTRTA AITGAYVALS DAVTWLAAAG RLSDPRPLSC AIAAVSVGVV DGRVRVDLPY 190 200 210 220 230 240 SEDSRAEVDM NVVATDTGTL VEIQGTGEGA TFPRSTLDKM LDAALGATEQ LFVLQREALD 250 APYPGVLPEG PAPKKAFGS

References

[1]	Fleischmann R.D., Dodson R.J., Haft D.H., Merkel J.S., Nelson W.C., Fraser C.M. Submitted (OCT-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 700084 / mc(2)155.
[2]	"Ortho-proteogenomics: multiple proteomes investigation through orthology and a new MS-based protocol." Gallien S., Perrodou E., Carapito C., Deshayes C., Reyrat J.-M., Van Dorsselaer A., Poch O., Schaeffer C., Lecompte O. Genome Res. 19:128-135(2009) [PubMed: 18955433] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000480 Genomic DNA. Translation: ABK70048.1.
RefSeq	YP_889156.1. NC_008596.1.
3D structure databases
ProteinModelPortal	A0R1W8.
SMR	A0R1W8. Positions 3-248.
ModBase	Search...
Protein-protein interaction databases
STRING	A0R1W8.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBMYCT00000044997; EBMYCP00000043334; EBMYCG00000044992.
GeneID	4532887.
GenomeReviews	Gene locus MSMEG_4901 in contig CP000480_GR.
KEGG	msm:MSMEG_4901.
NMPDR	fig|246196.1.peg.4886.
PATRIC	18082099. VBIMycSme59918_4782.
TIGR	MSMEG_4901.
Phylogenomic databases
eggNOG	COG0689.
GeneTree	EBGT00050000017525.
HOGENOM	HBG737187.
OMA	MLPRATG.
PhylomeDB	A0R1W8.
ProtClustDB	PRK00173.
Enzyme and pathway databases
BioCyc	MSME246196:MSMEG_4901-MONOMER.
Family and domain databases
HAMAP	MF_00564. RNase_PH. [Tree]
InterPro	IPR001247. ExoRNase_PH_dom1. IPR015847. ExoRNase_PH_dom2. IPR020568. Ribosomal_S5_D2-typ_fold. IPR002381. RNase_PH_bac-type. IPR018336. RNase_PH_CS. [Graphical view]
KO	K00989.
Pfam	PF01138. RNase_PH. 1 hit. PF03725. RNase_PH_C. 1 hit. [Graphical view]
SUPFAM	SSF55666. 3_ExoRNase. 1 hit. SSF54211. Ribosomal_S5_D2-typ_fold. 1 hit.
TIGRFAMs	TIGR01966. RNasePH. 1 hit.
PROSITE	PS01277. RIBONUCLEASE_PH. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

RNPH_MYCS2

Accession

Primary (citable) accession number: A0R1W8

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	January 9, 2007
Last modified:	December 14, 2011
This is version 36 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program