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Protein

Ribonuclease PH

Gene

rph

Organism
Mycobacterium smegmatis (strain ATCC 700084 / mc(2)155)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Phosphorolytic 3'-5' exoribonuclease that plays an important role in tRNA 3'-end maturation. Removes nucleotide residues following the 3'-CCA terminus of tRNAs; can also add nucleotides to the ends of RNA molecules by using nucleoside diphosphates as substrates, but this may not be physiologically important. Probably plays a role in initiation of 16S rRNA degradation (leading to ribosome degradation) during starvation.UniRule annotation

Catalytic activityi

tRNA(n+1) + phosphate = tRNA(n) + a nucleoside diphosphate.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei88Phosphate (substrate) bindingUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionNucleotidyltransferase, RNA-binding, Transferase, tRNA-binding
Biological processrRNA processing, tRNA processing

Enzyme and pathway databases

BioCyciMSME246196:G1H7P-4871-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
Ribonuclease PHUniRule annotation (EC:2.7.7.56UniRule annotation)
Short name:
RNase PHUniRule annotation
Alternative name(s):
tRNA nucleotidyltransferaseUniRule annotation
Gene namesi
Name:rphUniRule annotation
Ordered Locus Names:MSMEG_4901, MSMEI_4774
OrganismiMycobacterium smegmatis (strain ATCC 700084 / mc(2)155)
Taxonomic identifieri246196 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaCorynebacterialesMycobacteriaceaeMycobacteriumMycobacterium fortuitum complex
Proteomesi
  • UP000006158 Componenti: Chromosome
  • UP000000757 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_10000248301 – 259Ribonuclease PHAdd BLAST259

Interactioni

Subunit structurei

Homohexameric ring arranged as a trimer of dimers.UniRule annotation

Protein-protein interaction databases

STRINGi246196.MSMEG_4901

Structurei

3D structure databases

ProteinModelPortaliA0R1W8
SMRiA0R1W8
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni126 – 128Phosphate (substrate) bindingUniRule annotation3

Sequence similaritiesi

Belongs to the RNase PH family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105ED0 Bacteria
COG0689 LUCA
HOGENOMiHOG000229516
KOiK00989
OMAiKGKGQGW
OrthoDBiPOG091H03ML

Family and domain databases

CDDicd11362 RNase_PH_bact, 1 hit
Gene3Di3.30.230.70, 1 hit
HAMAPiMF_00564 RNase_PH, 1 hit
InterProiView protein in InterPro
IPR001247 ExoRNase_PH_dom1
IPR015847 ExoRNase_PH_dom2
IPR036345 ExoRNase_PH_dom2_sf
IPR027408 PNPase/RNase_PH_dom_sf
IPR020568 Ribosomal_S5_D2-typ_fold
IPR002381 RNase_PH_bac-type
IPR018336 RNase_PH_CS
PfamiView protein in Pfam
PF01138 RNase_PH, 1 hit
PF03725 RNase_PH_C, 1 hit
SUPFAMiSSF54211 SSF54211, 1 hit
SSF55666 SSF55666, 1 hit
TIGRFAMsiTIGR01966 RNasePH, 1 hit
PROSITEiView protein in PROSITE
PS01277 RIBONUCLEASE_PH, 1 hit

Sequencei

Sequence statusi: Complete.

A0R1W8-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSRREDGRLD DELRPVVITR GFTSHPAGSV LVEFGQTRVM CTASVTEGVP
60 70 80 90 100
RWRKGSGQGW LTAEYAMLPA ATHDRSDRES VKGRIGGRTQ EISRLIGRSL
110 120 130 140 150
RACIDLAALG ENTIAIDCDV LQADGGTRTA AITGAYVALS DAVTWLAAAG
160 170 180 190 200
RLSDPRPLSC AIAAVSVGVV DGRVRVDLPY SEDSRAEVDM NVVATDTGTL
210 220 230 240 250
VEIQGTGEGA TFPRSTLDKM LDAALGATEQ LFVLQREALD APYPGVLPEG

PAPKKAFGS
Length:259
Mass (Da):27,384
Last modified:January 9, 2007 - v1
Checksum:i0B705F2B90061CD2
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000480 Genomic DNA Translation: ABK70048.1
CP001663 Genomic DNA Translation: AFP41222.1
RefSeqiWP_011730177.1, NZ_CP009494.1
YP_889156.1, NC_008596.1

Genome annotation databases

EnsemblBacteriaiABK70048; ABK70048; MSMEG_4901
AFP41222; AFP41222; MSMEI_4774
GeneIDi4532887
KEGGimsb:LJ00_24235
msg:MSMEI_4774
msm:MSMEG_4901
PATRICifig|246196.19.peg.4782

Similar proteinsi

Entry informationi

Entry nameiRNPH_MYCS2
AccessioniPrimary (citable) accession number: A0R1W8
Secondary accession number(s): I7G6A0
Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: January 9, 2007
Last modified: May 23, 2018
This is version 73 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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