Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Alkyl hydroperoxide reductase C

Gene

MSMEG_4891

Organism
Mycobacterium smegmatis (strain ATCC 700084 / mc(2)155)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Thiol-specific peroxidase that catalyzes the reduction of hydrogen peroxide and organic hydroperoxides to water and alcohols, respectively. Plays a role in cell protection against oxidative stress by detoxifying peroxides. Together with AhpD, DlaT and Lpd, constitutes an NADH-dependent peroxidase active against hydrogen and alkyl peroxides as well as serving as a peroxynitrite reductase, thus protecting the bacterium against reactive nitrogen intermediates and oxidative stress generated by the host immune system. Does not however seem to play a role in detoxification of isoniazid.By similarity

Miscellaneous

The active site is a conserved redox-active cysteine residue, the peroxidatic cysteine (C(P)), which makes the nucleophilic attack on the peroxide substrate. The peroxide oxidizes the C(P)-SH to cysteine sulfenic acid (C(P)-SOH), which then reacts with another cysteine residue, the resolving cysteine (C(R)), to form a disulfide bridge. The disulfide is subsequently reduced by an appropriate electron donor to complete the catalytic cycle. In this typical 2-Cys peroxiredoxin, C(R) is provided by the other dimeric subunit to form an intersubunit disulfide. The disulfide can subsequently be reduced through a mixed disulfide with the C-terminal cysteine of AhpD, resolved by its second cysteine or by thioredoxin (TrxC).By similarity

Catalytic activityi

2 R'-SH + ROOH = R'-S-S-R' + H2O + ROH.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei61Cysteine sulfenic acid (-SOH) intermediateBy similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionAntioxidant, Oxidoreductase, Peroxidase
Biological processStress response

Names & Taxonomyi

Protein namesi
Recommended name:
Alkyl hydroperoxide reductase C (EC:1.11.1.15)
Alternative name(s):
Peroxiredoxin
Thioredoxin peroxidase
Gene namesi
Ordered Locus Names:MSMEG_4891, MSMEI_4766
OrganismiMycobacterium smegmatis (strain ATCC 700084 / mc(2)155)
Taxonomic identifieri246196 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaCorynebacterialesMycobacteriaceaeMycobacterium
Proteomesi
  • UP000006158 Componenti: Chromosome
  • UP000000757 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm By similarity

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003961111 – 195Alkyl hydroperoxide reductase CAdd BLAST195

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Cross-linki41Isoglutamyl lysine isopeptide (Lys-Gln) (interchain with Q-Cter in protein Pup)1 Publication
Disulfide bondi61Interchain (with C-133 in AhpD); transientBy similarity
Disulfide bondi61Interchain (with C-174); in linked formBy similarity
Disulfide bondi174Interchain (with C-61); in linked formBy similarity

Keywords - PTMi

Disulfide bond, Isopeptide bond, Ubl conjugation

Interactioni

Subunit structurei

Homodimer; disulfide-linked, upon oxidation. 6 homodimers assemble to form a ring-like dodecamer. Identified in a complex with AhpD, DlaT and Lpd.By similarity

Protein-protein interaction databases

STRINGi246196.MSMEG_4891.

Structurei

3D structure databases

ProteinModelPortaliA0R1V9.
SMRiA0R1V9.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini4 – 170ThioredoxinPROSITE-ProRule annotationAdd BLAST167

Sequence similaritiesi

Keywords - Domaini

Redox-active center

Phylogenomic databases

eggNOGiENOG4105D3R. Bacteria.
COG0450. LUCA.
HOGENOMiHOG000022343.
KOiK03386.
OMAiCPANWEE.
OrthoDBiPOG091H026G.

Family and domain databases

InterProiView protein in InterPro
IPR000866. AhpC/TSA.
IPR024706. Peroxiredoxin_AhpC-typ.
IPR012336. Thioredoxin-like_fold.
IPR013766. Thioredoxin_domain.
PfamiView protein in Pfam
PF00578. AhpC-TSA. 1 hit.
PIRSFiPIRSF000239. AHPC. 1 hit.
SUPFAMiSSF52833. SSF52833. 1 hit.
PROSITEiView protein in PROSITE
PS51352. THIOREDOXIN_2. 1 hit.

Sequencei

Sequence statusi: Complete.

A0R1V9-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MALLTIGDQF PEYDLTAVVG GDLSKVDAKQ PDDYFTRVTS KDYEGKWRII
60 70 80 90 100
FFWPKDFTFV CPTEIAAFGK LNEDFEDRDA KVLGVSVDNE FVHFQWRAQH
110 120 130 140 150
EDLKTLPFPM VSDLKRELTA ACGVLNADGV ADRATFIVDP NNEVQFVSVT
160 170 180 190
AGSVGRNVDE VLRVLDALQS DELCACNWKK GDPTINAGEL LAGAV
Length:195
Mass (Da):21,626
Last modified:January 9, 2007 - v1
Checksum:i556A5C17A5DA937F
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000480 Genomic DNA. Translation: ABK73669.1.
CP001663 Genomic DNA. Translation: AFP41215.1.
RefSeqiWP_011730169.1. NZ_CP009494.1.
YP_889147.1. NC_008596.1.

Genome annotation databases

EnsemblBacteriaiABK73669; ABK73669; MSMEG_4891.
AFP41215; AFP41215; MSMEI_4766.
GeneIDi4531887.
KEGGimsb:LJ00_24190.
msg:MSMEI_4766.
msm:MSMEG_4891.
PATRICifig|246196.56.peg.4874.

Similar proteinsi

Entry informationi

Entry nameiAHPC_MYCS2
AccessioniPrimary (citable) accession number: A0R1V9
Secondary accession number(s): I7FIL4
Entry historyiIntegrated into UniProtKB/Swiss-Prot: August 10, 2010
Last sequence update: January 9, 2007
Last modified: August 30, 2017
This is version 74 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families