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Protein

Alkyl hydroperoxide reductase subunit C

Gene

MSMEG_4891

Organism
Mycobacterium smegmatis (strain ATCC 700084 / mc(2)155)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Together with AhpD, DltA and Lpd constitutes an NADH-dependent peroxidase active against hydrogen and alkyl peroxides as well as serving as a peroxynitrite reductase, thus protecting the bacterium against reactive nitrogen intermediates and oxidative stress generated by the host immune system. Does not however seem to play a role in detoxification of isoniazid (By similarity).By similarity

Catalytic activityi

2 R'-SH + ROOH = R'-S-S-R' + H2O + ROH.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei61 – 611Cysteine sulfenic acid (-SOH) intermediateBy similarity

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Antioxidant, Oxidoreductase, Peroxidase

Keywords - Biological processi

Stress response

Enzyme and pathway databases

BioCyciMSME246196:GJ4Y-4890-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Alkyl hydroperoxide reductase subunit C (EC:1.11.1.15)
Alternative name(s):
Peroxiredoxin
Thioredoxin peroxidase
Gene namesi
Ordered Locus Names:MSMEG_4891, MSMEI_4766
OrganismiMycobacterium smegmatis (strain ATCC 700084 / mc(2)155)
Taxonomic identifieri246196 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaCorynebacterialesMycobacteriaceaeMycobacterium
Proteomesi
  • UP000006158 Componenti: Chromosome
  • UP000000757 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 195195Alkyl hydroperoxide reductase subunit CPRO_0000396111Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Cross-linki41 – 41Isoglutamyl lysine isopeptide (Lys-Gln) (interchain with Q-Cter in protein Pup)1 Publication
Disulfide bondi61 – 61Interchain (with C-174); in linked formBy similarity
Disulfide bondi174 – 174Interchain (with C-61); in linked formBy similarity

Post-translational modificationi

The Cys-61-SH group is the primary site of oxidation by H2O2, and the oxidized Cys-61 (probably Cys-SOH) rapidly reacts with Cys-174-SH of the other subunit to form an intermolecular disulfide. This disulfide is subsequently reduced by thioredoxin (By similarity).By similarity

Keywords - PTMi

Disulfide bond, Isopeptide bond, Ubl conjugation

Interactioni

Subunit structurei

Homodimer.By similarity

Protein-protein interaction databases

STRINGi246196.MSMEG_4891.

Structurei

3D structure databases

ProteinModelPortaliA0R1V9.
SMRiA0R1V9. Positions 3-179.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini4 – 170167ThioredoxinPROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the AhpC/TSA family.Curated
Contains 1 thioredoxin domain.PROSITE-ProRule annotation

Keywords - Domaini

Redox-active center

Phylogenomic databases

eggNOGiENOG4105D3R. Bacteria.
COG0450. LUCA.
HOGENOMiHOG000022343.
KOiK03386.
OMAiEFKKINC.
OrthoDBiEOG67X1XH.

Family and domain databases

Gene3Di3.40.30.10. 1 hit.
InterProiIPR000866. AhpC/TSA.
IPR024706. Peroxiredoxin_AhpC-typ.
IPR012336. Thioredoxin-like_fold.
IPR013766. Thioredoxin_domain.
[Graphical view]
PfamiPF00578. AhpC-TSA. 1 hit.
[Graphical view]
PIRSFiPIRSF000239. AHPC. 1 hit.
SUPFAMiSSF52833. SSF52833. 1 hit.
PROSITEiPS51352. THIOREDOXIN_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

A0R1V9-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MALLTIGDQF PEYDLTAVVG GDLSKVDAKQ PDDYFTRVTS KDYEGKWRII
60 70 80 90 100
FFWPKDFTFV CPTEIAAFGK LNEDFEDRDA KVLGVSVDNE FVHFQWRAQH
110 120 130 140 150
EDLKTLPFPM VSDLKRELTA ACGVLNADGV ADRATFIVDP NNEVQFVSVT
160 170 180 190
AGSVGRNVDE VLRVLDALQS DELCACNWKK GDPTINAGEL LAGAV
Length:195
Mass (Da):21,626
Last modified:January 9, 2007 - v1
Checksum:i556A5C17A5DA937F
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000480 Genomic DNA. Translation: ABK73669.1.
CP001663 Genomic DNA. Translation: AFP41215.1.
RefSeqiWP_011730169.1. NZ_CP009494.1.
YP_889147.1. NC_008596.1.

Genome annotation databases

EnsemblBacteriaiABK73669; ABK73669; MSMEG_4891.
AFP41215; AFP41215; MSMEI_4766.
GeneIDi4531887.
KEGGimsb:LJ00_24190.
msg:MSMEI_4766.
msm:MSMEG_4891.
PATRICi18082079. VBIMycSme59918_4773.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000480 Genomic DNA. Translation: ABK73669.1.
CP001663 Genomic DNA. Translation: AFP41215.1.
RefSeqiWP_011730169.1. NZ_CP009494.1.
YP_889147.1. NC_008596.1.

3D structure databases

ProteinModelPortaliA0R1V9.
SMRiA0R1V9. Positions 3-179.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi246196.MSMEG_4891.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABK73669; ABK73669; MSMEG_4891.
AFP41215; AFP41215; MSMEI_4766.
GeneIDi4531887.
KEGGimsb:LJ00_24190.
msg:MSMEI_4766.
msm:MSMEG_4891.
PATRICi18082079. VBIMycSme59918_4773.

Phylogenomic databases

eggNOGiENOG4105D3R. Bacteria.
COG0450. LUCA.
HOGENOMiHOG000022343.
KOiK03386.
OMAiEFKKINC.
OrthoDBiEOG67X1XH.

Enzyme and pathway databases

BioCyciMSME246196:GJ4Y-4890-MONOMER.

Family and domain databases

Gene3Di3.40.30.10. 1 hit.
InterProiIPR000866. AhpC/TSA.
IPR024706. Peroxiredoxin_AhpC-typ.
IPR012336. Thioredoxin-like_fold.
IPR013766. Thioredoxin_domain.
[Graphical view]
PfamiPF00578. AhpC-TSA. 1 hit.
[Graphical view]
PIRSFiPIRSF000239. AHPC. 1 hit.
SUPFAMiSSF52833. SSF52833. 1 hit.
PROSITEiPS51352. THIOREDOXIN_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. Fleischmann R.D., Dodson R.J., Haft D.H., Merkel J.S., Nelson W.C., Fraser C.M.
    Submitted (OCT-2006) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 700084 / mc(2)155.
  2. "Interrupted coding sequences in Mycobacterium smegmatis: authentic mutations or sequencing errors?"
    Deshayes C., Perrodou E., Gallien S., Euphrasie D., Schaeffer C., Van-Dorsselaer A., Poch O., Lecompte O., Reyrat J.-M.
    Genome Biol. 8:R20.1-R20.9(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 700084 / mc(2)155.
  3. "Ortho-proteogenomics: multiple proteomes investigation through orthology and a new MS-based protocol."
    Gallien S., Perrodou E., Carapito C., Deshayes C., Reyrat J.-M., Van Dorsselaer A., Poch O., Schaeffer C., Lecompte O.
    Genome Res. 19:128-135(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 700084 / mc(2)155.
  4. Cited for: PUPYLATION AT LYS-41, IDENTIFICATION BY MASS SPECTROMETRY.

Entry informationi

Entry nameiAHPC_MYCS2
AccessioniPrimary (citable) accession number: A0R1V9
Secondary accession number(s): I7FIL4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 10, 2010
Last sequence update: January 9, 2007
Last modified: July 6, 2016
This is version 66 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.