ID   MHPB_MYCS2              Reviewed;         313 AA.
AC   A0R1T3;
DT   20-MAY-2008, integrated into UniProtKB/Swiss-Prot.
DT   20-MAY-2008, sequence version 2.
DT   16-JUN-2009, entry version 21.
DE   RecName: Full=2,3-dihydroxyphenylpropionate/2,3-dihydroxicinnamic acid 1,2-dioxygenase;
DE            EC=1.13.11.16;
GN   Name=mhpB; OrderedLocusNames=MSMEG_4865;
OS   Mycobacterium smegmatis (strain ATCC 700084 / mc(2)155).
OC   Bacteria; Actinobacteria; Actinobacteridae; Actinomycetales;
OC   Corynebacterineae; Mycobacteriaceae; Mycobacterium.
OX   NCBI_TaxID=246196;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA   Fleischmann R.D., Dodson R.J., Haft D.H., Merkel J.S., Nelson W.C.,
RA   Fraser C.M.;
RL   Submitted (OCT-2006) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Catalyzes the non-heme iron(II)-dependent oxidative
CC       cleavage of 2,3-dihydroxyphenylpropionic acid and 2,3-
CC       dihydroxicinnamic acid into 2-hydroxy-6-ketononadienedioate and 2-
CC       hydroxy-6-ketononatrienedioate, respectively (By similarity).
CC   -!- CATALYTIC ACTIVITY: 3-(2,3-dihydroxyphenyl)propanoate + O(2) = 2-
CC       hydroxy-6-oxonona-2,4-diene-1,9-dioate.
CC   -!- CATALYTIC ACTIVITY: 2,3-dihydroxicinnamic acid + O(2) = 2-hydroxy-
CC       6-oxonona-2,4,7-triene-1,9-dioate.
CC   -!- COFACTOR: Fe(2+) ion (By similarity).
CC   -!- PATHWAY: Aromatic compound metabolism; 3-phenylpropionic acid
CC       degradation.
CC   -!- SUBUNIT: Homotetramer (By similarity).
CC   -!- SIMILARITY: Belongs to the ligB/mhpB extradiol dioxygenase family.
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DR   EMBL; CP000480; ABK71791.1; ALT_INIT; Genomic_DNA.
DR   RefSeq; YP_889121.1; -.
DR   GeneID; 4531580; -.
DR   GenomeReviews; CP000480_GR; MSMEG_4865.
DR   KEGG; msm:MSMEG_4865; -.
DR   NMPDR; fig|246196.1.peg.4851; -.
DR   TIGR; MSMEG_4865; -.
DR   GO; GO:0008669; F:2,3-dihydroxy-phenylpropionate 1,2-dioxygen...; IEA:HAMAP.
DR   GO; GO:0047070; F:3-carboxyethylcatechol 2,3-dioxygenase acti...; IEA:EC.
DR   GO; GO:0008198; F:ferrous iron binding; IEA:InterPro.
DR   GO; GO:0019439; P:aromatic compound catabolic process; IEA:HAMAP.
DR   GO; GO:0055114; P:oxidation reduction; IEA:UniProtKB-KW.
DR   HAMAP; MF_01653; -; 1.
DR   InterPro; IPR004183; Xdiol_dOase_3B.
DR   Pfam; PF02900; LigB; 1.
PE   3: Inferred from homology;
KW   Aromatic hydrocarbons catabolism; Complete proteome; Dioxygenase;
KW   Iron; Oxidoreductase.
FT   CHAIN         1    313       2,3-dihydroxyphenylpropionate/2,3-
FT                                dihydroxicinnamic acid 1,2-dioxygenase.
FT                                /FTId=PRO_0000337655.
FT   ACT_SITE    115    115       Proton donor (By similarity).
FT   ACT_SITE    179    179       Proton acceptor (By similarity).
SQ   SEQUENCE   313 AA;  33282 MW;  82D348B141FCF2AE CRC64;
     MPLALCCMSH SPLLNLPGPS AELLDQITDA IADARKFVEQ FDPELVVTFS PDHYNGFFYR
     LMPPFCIGTA AAGVGDYGTY QGPLPVDADI ANACAESLWE SGVDIAISTA MDVDHGTVQP
     LQELFGDATA RPVVPIFINS VATPLGPLSR SRALGAAVGT FLATLDKRVL IVGSGGLSHD
     PPVPTLATAP PAALDRIVHG APMTPEQRMA RQEAVIKAAH DFAHGQSPLR SLNPDWDRSL
     LEIFDEGRLS DLDGWTNTFI TGEGGNSAHE IRTWVAAFAA LAAHGEYQTG NHFYRAAPEL
     IAGFAIRTAV PST
//