A0R1T3 (MHPB_MYCS2) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
2,3-dihydroxyphenylpropionate/2,3-dihydroxicinnamic acid 1,2-dioxygenase
EC=1.13.11.16

Gene names

Name:	mhpB
Ordered Locus Names:	MSMEG_4865

Organism

Mycobacterium smegmatis (strain ATCC 700084 / mc(2)155)

Taxonomic identifier

246196 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Corynebacterineae › Mycobacteriaceae › Mycobacterium

Protein attributes

Sequence length	313 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the non-heme iron(II)-dependent oxidative cleavage of 2,3-dihydroxyphenylpropionic acid and 2,3-dihydroxicinnamic acid into 2-hydroxy-6-ketononadienedioate and 2-hydroxy-6-ketononatrienedioate, respectively By similarity. HAMAP MF_01653
Catalytic activity	3-(2,3-dihydroxyphenyl)propanoate + O2 = 2-hydroxy-6-oxonona-2,4-diene-1,9-dioate. HAMAP MF_01653 (2E)-3-(2,3-dihydroxyphenyl)prop-2-enoate + O2 = 2-hydroxy-6-oxonona-2,4,7-triene-1,9-dioate. HAMAP MF_01653
Cofactor	Fe2+ ion By similarity. HAMAP MF_01653
Pathway	Aromatic compound metabolism; 3-phenylpropanoate degradation. HAMAP MF_01653
Subunit structure	Homotetramer By similarity. HAMAP MF_01653
Sequence similarities	Belongs to the ligB/mhpB extradiol dioxygenase family.
Sequence caution	The sequence ABK71791.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Biological process	Aromatic hydrocarbons catabolism
Ligand	Iron
Molecular function	Dioxygenase Oxidoreductase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	aromatic compound catabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	3-carboxyethylcatechol 2,3-dioxygenase activity Inferred from electronic annotation. Source: EC ferrous iron binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 313	313	2,3-dihydroxyphenylpropionate/2,3-dihydroxicinnamic acid 1,2-dioxygenase HAMAP MF_01653		PRO_0000337655
Sites
	Active site	115	1	Proton donor By similarity
	Active site	179	1	Proton acceptor By similarity

Sequences

	Sequence		Length	Mass (Da)
	A0R1T3 [UniParc]. Last modified May 20, 2008. Version 2. Checksum: 82D348B141FCF2AE	FASTA	313	33,282
	10 20 30 40 50 60 MPLALCCMSH SPLLNLPGPS AELLDQITDA IADARKFVEQ FDPELVVTFS PDHYNGFFYR 70 80 90 100 110 120 LMPPFCIGTA AAGVGDYGTY QGPLPVDADI ANACAESLWE SGVDIAISTA MDVDHGTVQP 130 140 150 160 170 180 LQELFGDATA RPVVPIFINS VATPLGPLSR SRALGAAVGT FLATLDKRVL IVGSGGLSHD 190 200 210 220 230 240 PPVPTLATAP PAALDRIVHG APMTPEQRMA RQEAVIKAAH DFAHGQSPLR SLNPDWDRSL 250 260 270 280 290 300 LEIFDEGRLS DLDGWTNTFI TGEGGNSAHE IRTWVAAFAA LAAHGEYQTG NHFYRAAPEL 310 IAGFAIRTAV PST

References

[1]	Fleischmann R.D., Dodson R.J., Haft D.H., Merkel J.S., Nelson W.C., Fraser C.M. Submitted (OCT-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 700084 / mc(2)155.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000480 Genomic DNA. Translation: ABK71791.1. Different initiation.
RefSeq	YP_889121.1. NC_008596.1.
3D structure databases
ProteinModelPortal	A0R1T3.
ModBase	Search...
Protein-protein interaction databases
STRING	A0R1T3.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBMYCT00000040915; EBMYCP00000039252; EBMYCG00000040910.
GeneID	4531580.
GenomeReviews	Gene locus MSMEG_4865 in contig CP000480_GR.
KEGG	msm:MSMEG_4865.
NMPDR	fig|246196.1.peg.4851.
PATRIC	18082027. VBIMycSme59918_4747.
TIGR	MSMEG_4865.
Phylogenomic databases
eggNOG	NOG42398.
GeneTree	EBGT00050000017557.
HOGENOM	HBG565586.
ProtClustDB	PRK13370.
Enzyme and pathway databases
BioCyc	MSME246196:MSMEG_4865-MONOMER.
Family and domain databases
HAMAP	MF_01653. MhpB. [Tree]
InterPro	IPR023789. DHPP/DHXA_dioxygenase. IPR004183. Xdiol_dOase_suB. [Graphical view]
Gene3D	G3DSA:3.40.830.10. Xdiol_dOase_3B. 2 hits.
KO	K05713.
Pfam	PF02900. LigB. 1 hit. [Graphical view]
SUPFAM	SSF53213. Xdiol_dOase_3B. 1 hit.
ProtoNet	Search...

Entry information

Entry name

MHPB_MYCS2

Accession

Primary (citable) accession number: A0R1T3

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 20, 2008
Last sequence update:	May 20, 2008
Last modified:	January 25, 2012
This is version 39 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program