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A0R148

- PROB_MYCS2

UniProt

A0R148 - PROB_MYCS2

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Protein

Glutamate 5-kinase

Gene
proB, MSMEG_4621, MSMEI_4505
Organism
Mycobacterium smegmatis (strain ATCC 700084 / mc(2)155)
Status
Reviewed - Annotation score: 3 out of 5 - Experimental evidence at protein leveli

Functioni

Catalyzes the transfer of a phosphate group to glutamate to form L-glutamate 5-phosphate By similarity.UniRule annotation

Catalytic activityi

ATP + L-glutamate = ADP + L-glutamate 5-phosphate.UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei17 – 171ATP By similarity
Binding sitei57 – 571Substrate By similarity
Binding sitei144 – 1441Substrate By similarity
Binding sitei156 – 1561Substrate; via amide nitrogen By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi176 – 1772ATP By similarity
Nucleotide bindingi220 – 2267ATP By similarity

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. glutamate 5-kinase activity Source: UniProtKB-HAMAP
  3. RNA binding Source: InterPro

GO - Biological processi

  1. L-proline biosynthetic process Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Biological processi

Amino-acid biosynthesis, Proline biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciMSME246196:GJ4Y-4621-MONOMER.
UniPathwayiUPA00098; UER00359.

Names & Taxonomyi

Protein namesi
Recommended name:
Glutamate 5-kinase (EC:2.7.2.11)
Alternative name(s):
Gamma-glutamyl kinase
Short name:
GK
Gene namesi
Name:proB
Ordered Locus Names:MSMEG_4621, MSMEI_4505
OrganismiMycobacterium smegmatis (strain ATCC 700084 / mc(2)155)
Taxonomic identifieri246196 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaActinobacteridaeActinomycetalesCorynebacterineaeMycobacteriaceaeMycobacterium
ProteomesiUP000000757: Chromosome, UP000006158: Chromosome

Subcellular locationi

Cytoplasm By similarity UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11Removed1 Publication
Chaini2 – 370369Glutamate 5-kinaseUniRule annotationPRO_1000081076Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi246196.MSMEG_4621.

Structurei

3D structure databases

ProteinModelPortaliA0R148.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini282 – 36079PUAAdd
BLAST

Sequence similaritiesi

Contains 1 PUA domain.

Phylogenomic databases

eggNOGiCOG0263.
HOGENOMiHOG000246368.
KOiK00931.
OMAiFDAKEIP.
OrthoDBiEOG6PGK7G.

Family and domain databases

Gene3Di2.30.130.10. 1 hit.
3.40.1160.10. 1 hit.
HAMAPiMF_00456. ProB.
InterProiIPR001048. Asp/Glu/Uridylate_kinase.
IPR001057. Glu/AcGlu_kinase.
IPR011529. Glu_5kinase.
IPR005715. Glu_5kinase/COase_Synthase.
IPR019797. Glutamate_5-kinase_CS.
IPR002478. PUA.
IPR015947. PUA-like_domain.
[Graphical view]
PfamiPF00696. AA_kinase. 1 hit.
PF01472. PUA. 1 hit.
[Graphical view]
PIRSFiPIRSF000729. GK. 1 hit.
PRINTSiPR00474. GLU5KINASE.
SMARTiSM00359. PUA. 1 hit.
[Graphical view]
SUPFAMiSSF53633. SSF53633. 1 hit.
SSF88697. SSF88697. 1 hit.
TIGRFAMsiTIGR01027. proB. 1 hit.
PROSITEiPS00902. GLUTAMATE_5_KINASE. 1 hit.
PS50890. PUA. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

A0R148-1 [UniParc]FASTAAdd to Basket

« Hide

MSEHREAVRT ARSVVVKIGT TALTTPSGVF DANRLASLVE AIEGRMKAGS    50
DVVIVSSGAI AAGIEPLGLS KRPTDLATKQ AAASVGQVAL VNAWSAAFAV 100
YNRTVGQVLL TAHDISMRVQ HNNAQRTLDR LRALHAVAIV NENDTVATNE 150
IRFGDNDRLS ALVAHLVGAD ALILLSDIDG LYDGDPRKAT PDKPARFIPE 200
VAAQGDLDGV VAGRGSSLGT GGMASKLSSA LLAADAGVPV LLAAAADAGR 250
ALDDASVGTV FAPRPERMSA RKFWMRYAAE SAGALTLDDG AVRAVIKQRR 300
SLLPAGITSV TGRFHGGDVV DLRALDGHTV ARGVVAYDQA ELASIIGRST 350
HELPVEMRRP AVHADDLVRT 370
Length:370
Mass (Da):38,607
Last modified:January 9, 2007 - v1
Checksum:i878693E7975F7CFB
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000480 Genomic DNA. Translation: ABK71775.1.
CP001663 Genomic DNA. Translation: AFP40959.1.
RefSeqiWP_011729969.1. NC_018289.1.
YP_006569254.1. NC_018289.1.
YP_888886.1. NC_008596.1.

Genome annotation databases

EnsemblBacteriaiABK71775; ABK71775; MSMEG_4621.
AFP40959; AFP40959; MSMEI_4505.
GeneIDi4531097.
KEGGimsg:MSMEI_4505.
msm:MSMEG_4621.
PATRICi18081557. VBIMycSme59918_4517.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000480 Genomic DNA. Translation: ABK71775.1 .
CP001663 Genomic DNA. Translation: AFP40959.1 .
RefSeqi WP_011729969.1. NC_018289.1.
YP_006569254.1. NC_018289.1.
YP_888886.1. NC_008596.1.

3D structure databases

ProteinModelPortali A0R148.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 246196.MSMEG_4621.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ABK71775 ; ABK71775 ; MSMEG_4621 .
AFP40959 ; AFP40959 ; MSMEI_4505 .
GeneIDi 4531097.
KEGGi msg:MSMEI_4505.
msm:MSMEG_4621.
PATRICi 18081557. VBIMycSme59918_4517.

Phylogenomic databases

eggNOGi COG0263.
HOGENOMi HOG000246368.
KOi K00931.
OMAi FDAKEIP.
OrthoDBi EOG6PGK7G.

Enzyme and pathway databases

UniPathwayi UPA00098 ; UER00359 .
BioCyci MSME246196:GJ4Y-4621-MONOMER.

Family and domain databases

Gene3Di 2.30.130.10. 1 hit.
3.40.1160.10. 1 hit.
HAMAPi MF_00456. ProB.
InterProi IPR001048. Asp/Glu/Uridylate_kinase.
IPR001057. Glu/AcGlu_kinase.
IPR011529. Glu_5kinase.
IPR005715. Glu_5kinase/COase_Synthase.
IPR019797. Glutamate_5-kinase_CS.
IPR002478. PUA.
IPR015947. PUA-like_domain.
[Graphical view ]
Pfami PF00696. AA_kinase. 1 hit.
PF01472. PUA. 1 hit.
[Graphical view ]
PIRSFi PIRSF000729. GK. 1 hit.
PRINTSi PR00474. GLU5KINASE.
SMARTi SM00359. PUA. 1 hit.
[Graphical view ]
SUPFAMi SSF53633. SSF53633. 1 hit.
SSF88697. SSF88697. 1 hit.
TIGRFAMsi TIGR01027. proB. 1 hit.
PROSITEi PS00902. GLUTAMATE_5_KINASE. 1 hit.
PS50890. PUA. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Fleischmann R.D., Dodson R.J., Haft D.H., Merkel J.S., Nelson W.C., Fraser C.M.
    Submitted (OCT-2006) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 700084 / mc(2)155.
  2. "Interrupted coding sequences in Mycobacterium smegmatis: authentic mutations or sequencing errors?"
    Deshayes C., Perrodou E., Gallien S., Euphrasie D., Schaeffer C., Van-Dorsselaer A., Poch O., Lecompte O., Reyrat J.-M.
    Genome Biol. 8:R20.1-R20.9(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 700084 / mc(2)155.
  3. "Ortho-proteogenomics: multiple proteomes investigation through orthology and a new MS-based protocol."
    Gallien S., Perrodou E., Carapito C., Deshayes C., Reyrat J.-M., Van Dorsselaer A., Poch O., Schaeffer C., Lecompte O.
    Genome Res. 19:128-135(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS], CLEAVAGE OF INITIATOR METHIONINE.
    Strain: ATCC 700084 / mc(2)155.

Entry informationi

Entry nameiPROB_MYCS2
AccessioniPrimary (citable) accession number: A0R148
Secondary accession number(s): I7GDU5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 20, 2008
Last sequence update: January 9, 2007
Last modified: September 3, 2014
This is version 63 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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