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Protein

Gamma-glutamyl phosphate reductase

Gene

proA

Organism
Mycobacterium smegmatis (strain ATCC 700084 / mc(2)155)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the NADPH-dependent reduction of L-glutamate 5-phosphate into L-glutamate 5-semialdehyde and phosphate. The product spontaneously undergoes cyclization to form 1-pyrroline-5-carboxylate.UniRule annotation

Catalytic activityi

L-glutamate 5-semialdehyde + phosphate + NADP+ = L-glutamyl 5-phosphate + NADPH.UniRule annotation

Pathwayi: L-proline biosynthesis

This protein is involved in step 2 of the subpathway that synthesizes L-glutamate 5-semialdehyde from L-glutamate.UniRule annotation
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. Glutamate 5-kinase (proB)
  2. Gamma-glutamyl phosphate reductase (proA)
This subpathway is part of the pathway L-proline biosynthesis, which is itself part of Amino-acid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes L-glutamate 5-semialdehyde from L-glutamate, the pathway L-proline biosynthesis and in Amino-acid biosynthesis.

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Amino-acid biosynthesis, Proline biosynthesis

Keywords - Ligandi

NADP

Enzyme and pathway databases

BioCyciMSME246196:GJ4Y-4583-MONOMER.
UniPathwayiUPA00098; UER00360.

Names & Taxonomyi

Protein namesi
Recommended name:
Gamma-glutamyl phosphate reductaseUniRule annotation (EC:1.2.1.41UniRule annotation)
Short name:
GPRUniRule annotation
Alternative name(s):
Glutamate-5-semialdehyde dehydrogenaseUniRule annotation
Glutamyl-gamma-semialdehyde dehydrogenaseUniRule annotation
Short name:
GSA dehydrogenaseUniRule annotation
Gene namesi
Name:proAUniRule annotation
Ordered Locus Names:MSMEG_4584, MSMEI_4472
OrganismiMycobacterium smegmatis (strain ATCC 700084 / mc(2)155)
Taxonomic identifieri246196 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaCorynebacterialesMycobacteriaceaeMycobacterium
Proteomesi
  • UP000006158 Componenti: Chromosome
  • UP000000757 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 424424Gamma-glutamyl phosphate reductasePRO_0000340896Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi246196.MSMEG_4584.

Structurei

3D structure databases

ProteinModelPortaliA0R115.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the gamma-glutamyl phosphate reductase family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105C2S. Bacteria.
COG0014. LUCA.
HOGENOMiHOG000246356.
KOiK00147.
OMAiCNAIETL.
OrthoDBiEOG6FFSCX.

Family and domain databases

Gene3Di3.40.309.10. 1 hit.
3.40.605.10. 2 hits.
HAMAPiMF_00412. ProA.
InterProiIPR016161. Ald_DH/histidinol_DH.
IPR016163. Ald_DH_C.
IPR016162. Ald_DH_N.
IPR015590. Aldehyde_DH_dom.
IPR020593. G-glutamylP_reductase_CS.
IPR012134. Glu-5-SA_DH.
IPR000965. GPR_dom.
[Graphical view]
PfamiPF00171. Aldedh. 2 hits.
[Graphical view]
PIRSFiPIRSF000151. GPR. 1 hit.
SUPFAMiSSF53720. SSF53720. 1 hit.
TIGRFAMsiTIGR00407. proA. 1 hit.
PROSITEiPS01223. PROA. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

A0R115-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSVEAQSRSG AVDTQEPADL REQVHSAARR ARVAARTLAT LSAEAKNRAL
60 70 80 90 100
HAAADSVLAN VDAVLAANAA DVDAARQGGT PEAMIDRLAL NPQRVDGIAA
110 120 130 140 150
GLRQVAALPD PVGEVLRGKT LPNGLQLRQQ RVPLGVVGMV YEGRPNVTVD
160 170 180 190 200
AFGLTLKSGN AALLRGSSSA ARSNQALVDA LRSALAEEGL PLDAVQLLPS
210 220 230 240 250
QDRASVTHLI QARGLVDVVI PRGGAGLIDA VVRDAQVPTI ETGVGNCHVY
260 270 280 290 300
VHSSADIDMA EKILLNAKTR RPSVCNAAET LLVDRALTDT ALPRLVKALQ
310 320 330 340 350
DAGVTVHADP TEDELRAEFL SMDIALAVVD GLDAAIDHIN TYGTGHTEAI
360 370 380 390 400
VTTDLAAAQR FTERVDAAAV MVNASTSFTD GEQFGFGAEI GISTQKLHAR
410 420
GPMGLPELTS TKWIVWGDGQ IRPA
Length:424
Mass (Da):44,375
Last modified:January 9, 2007 - v1
Checksum:i01BD92B35C94C228
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000480 Genomic DNA. Translation: ABK73874.1.
CP001663 Genomic DNA. Translation: AFP40926.1.
RefSeqiWP_011729942.1. NZ_CP009494.1.
YP_888853.1. NC_008596.1.

Genome annotation databases

EnsemblBacteriaiABK73874; ABK73874; MSMEG_4584.
AFP40926; AFP40926; MSMEI_4472.
GeneIDi4536453.
KEGGimsb:LJ00_22685.
msg:MSMEI_4472.
msm:MSMEG_4584.
PATRICi18081497. VBIMycSme59918_4487.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000480 Genomic DNA. Translation: ABK73874.1.
CP001663 Genomic DNA. Translation: AFP40926.1.
RefSeqiWP_011729942.1. NZ_CP009494.1.
YP_888853.1. NC_008596.1.

3D structure databases

ProteinModelPortaliA0R115.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi246196.MSMEG_4584.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABK73874; ABK73874; MSMEG_4584.
AFP40926; AFP40926; MSMEI_4472.
GeneIDi4536453.
KEGGimsb:LJ00_22685.
msg:MSMEI_4472.
msm:MSMEG_4584.
PATRICi18081497. VBIMycSme59918_4487.

Phylogenomic databases

eggNOGiENOG4105C2S. Bacteria.
COG0014. LUCA.
HOGENOMiHOG000246356.
KOiK00147.
OMAiCNAIETL.
OrthoDBiEOG6FFSCX.

Enzyme and pathway databases

UniPathwayiUPA00098; UER00360.
BioCyciMSME246196:GJ4Y-4583-MONOMER.

Family and domain databases

Gene3Di3.40.309.10. 1 hit.
3.40.605.10. 2 hits.
HAMAPiMF_00412. ProA.
InterProiIPR016161. Ald_DH/histidinol_DH.
IPR016163. Ald_DH_C.
IPR016162. Ald_DH_N.
IPR015590. Aldehyde_DH_dom.
IPR020593. G-glutamylP_reductase_CS.
IPR012134. Glu-5-SA_DH.
IPR000965. GPR_dom.
[Graphical view]
PfamiPF00171. Aldedh. 2 hits.
[Graphical view]
PIRSFiPIRSF000151. GPR. 1 hit.
SUPFAMiSSF53720. SSF53720. 1 hit.
TIGRFAMsiTIGR00407. proA. 1 hit.
PROSITEiPS01223. PROA. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Fleischmann R.D., Dodson R.J., Haft D.H., Merkel J.S., Nelson W.C., Fraser C.M.
    Submitted (OCT-2006) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 700084 / mc(2)155.
  2. "Interrupted coding sequences in Mycobacterium smegmatis: authentic mutations or sequencing errors?"
    Deshayes C., Perrodou E., Gallien S., Euphrasie D., Schaeffer C., Van-Dorsselaer A., Poch O., Lecompte O., Reyrat J.-M.
    Genome Biol. 8:R20.1-R20.9(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 700084 / mc(2)155.
  3. "Ortho-proteogenomics: multiple proteomes investigation through orthology and a new MS-based protocol."
    Gallien S., Perrodou E., Carapito C., Deshayes C., Reyrat J.-M., Van Dorsselaer A., Poch O., Schaeffer C., Lecompte O.
    Genome Res. 19:128-135(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 700084 / mc(2)155.

Entry informationi

Entry nameiPROA_MYCS2
AccessioniPrimary (citable) accession number: A0R115
Secondary accession number(s): I7GCI9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 10, 2008
Last sequence update: January 9, 2007
Last modified: November 11, 2015
This is version 75 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.