A0R115 (PROA_MYCS2) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Gamma-glutamyl phosphate reductase
Short name=GPR
EC=1.2.1.41

Alternative name(s):
Glutamate-5-semialdehyde dehydrogenase
Glutamyl-gamma-semialdehyde dehydrogenase
Short name=GSA dehydrogenase

Gene names

Name:	proA
Ordered Locus Names:	MSMEG_4584

Organism

Mycobacterium smegmatis (strain ATCC 700084 / mc(2)155)

Taxonomic identifier

246196 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Corynebacterineae › Mycobacteriaceae › Mycobacterium

Protein attributes

Sequence length	424 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the NADPH dependent reduction of L-gamma-glutamyl 5-phosphate into L-glutamate 5-semialdehyde and phosphate. The product spontaneously undergoes cyclization to form 1-pyrroline-5-carboxylate By similarity. HAMAP MF_00412
Catalytic activity	L-glutamate 5-semialdehyde + phosphate + NADP+ = L-glutamyl 5-phosphate + NADPH. HAMAP MF_00412
Pathway	Amino-acid biosynthesis; L-proline biosynthesis; L-glutamate 5-semialdehyde from L-glutamate: step 2/2. HAMAP MF_00412
Subcellular location	Cytoplasm By similarity HAMAP MF_00412.
Sequence similarities	Belongs to the gamma-glutamyl phosphate reductase family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Proline biosynthesis
Cellular component	Cytoplasm
Ligand	NADP
Molecular function	Oxidoreductase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	proline biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	NADP binding Inferred from electronic annotation. Source: InterPro glutamate-5-semialdehyde dehydrogenase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 424	424	Gamma-glutamyl phosphate reductase HAMAP MF_00412		PRO_0000340896

Sequences

	Sequence		Length	Mass (Da)
	A0R115 [UniParc]. Last modified January 9, 2007. Version 1. Checksum: 01BD92B35C94C228	FASTA	424	44,375
	10 20 30 40 50 60 MSVEAQSRSG AVDTQEPADL REQVHSAARR ARVAARTLAT LSAEAKNRAL HAAADSVLAN 70 80 90 100 110 120 VDAVLAANAA DVDAARQGGT PEAMIDRLAL NPQRVDGIAA GLRQVAALPD PVGEVLRGKT 130 140 150 160 170 180 LPNGLQLRQQ RVPLGVVGMV YEGRPNVTVD AFGLTLKSGN AALLRGSSSA ARSNQALVDA 190 200 210 220 230 240 LRSALAEEGL PLDAVQLLPS QDRASVTHLI QARGLVDVVI PRGGAGLIDA VVRDAQVPTI 250 260 270 280 290 300 ETGVGNCHVY VHSSADIDMA EKILLNAKTR RPSVCNAAET LLVDRALTDT ALPRLVKALQ 310 320 330 340 350 360 DAGVTVHADP TEDELRAEFL SMDIALAVVD GLDAAIDHIN TYGTGHTEAI VTTDLAAAQR 370 380 390 400 410 420 FTERVDAAAV MVNASTSFTD GEQFGFGAEI GISTQKLHAR GPMGLPELTS TKWIVWGDGQ IRPA

References

[1]	Fleischmann R.D., Dodson R.J., Haft D.H., Merkel J.S., Nelson W.C., Fraser C.M. Submitted (OCT-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 700084 / mc(2)155.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000480 Genomic DNA. Translation: ABK73874.1.
RefSeq	YP_888853.1. NC_008596.1.
3D structure databases
ProteinModelPortal	A0R115.
ModBase	Search...
Protein-protein interaction databases
STRING	A0R115.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBMYCT00000044733; EBMYCP00000043070; EBMYCG00000044728.
GeneID	4536453.
GenomeReviews	Gene locus MSMEG_4584 in contig CP000480_GR.
KEGG	msm:MSMEG_4584.
PATRIC	18081497. VBIMycSme59918_4487.
TIGR	MSMEG_4584.
Phylogenomic databases
eggNOG	COG0014.
GeneTree	EBGT00050000015663.
HOGENOM	HBG318080.
OMA	QYPAACN.
PhylomeDB	A0R115.
ProtClustDB	PRK00197.
Enzyme and pathway databases
BioCyc	MSME246196:MSMEG_4584-MONOMER.
Family and domain databases
HAMAP	MF_00412. ProA. [Tree]
InterPro	IPR016161. Ald_DH/histidinol_DH. IPR016163. Ald_DH_C. IPR016162. Ald_DH_N. IPR015590. Aldehyde_DH_dom. IPR000965. G-glutamylP_reductase. IPR020593. G-glutamylP_reductase_CS. IPR012134. Glu-5-SA_DH. [Graphical view]
Gene3D	G3DSA:3.40.309.10. Aldehyde_dehydrogenase_C. 1 hit. G3DSA:3.40.605.10. Aldehyde_dehydrogenase_N. 2 hits.
KO	K00147.
PANTHER	PTHR11063:SF1. GSA_DH. 1 hit.
Pfam	PF00171. Aldedh. 2 hits. [Graphical view]
PIRSF	PIRSF000151. GPR. 1 hit.
SUPFAM	SSF53720. Aldehyde_DH/Histidinol_DH. 1 hit.
TIGRFAMs	TIGR00407. ProA. 1 hit.
PROSITE	PS01223. PROA. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PROA_MYCS2

Accession

Primary (citable) accession number: A0R115

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 10, 2008
Last sequence update:	January 9, 2007
Last modified:	December 14, 2011
This is version 47 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program