A0R0B2 (FABD_MYCS2) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Malonyl CoA-acyl carrier protein transacylase
Short name=MCT
EC=2.3.1.39

Gene names

Name:	fabD
Ordered Locus Names:	MSMEG_4325, MSMEI_4225

Organism

Mycobacterium smegmatis (strain ATCC 700084 / mc(2)155) [Reference proteome] [HAMAP]

Taxonomic identifier

246196 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Actinobacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Corynebacterineae › Mycobacteriaceae › Mycobacterium › Mycobacterium smegmatis

Protein attributes

Sequence length	290 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Catalytic activity	Malonyl-CoA + [acyl-carrier-protein] = CoA + malonyl-[acyl-carrier-protein].
Pathway	Lipid metabolism; fatty acid biosynthesis.
Sequence similarities	Belongs to the FabD family.
Sequence caution	The sequence AFP40681.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.

Ontologies

Keywords
Biological process	Fatty acid biosynthesis Fatty acid metabolism Lipid biosynthesis Lipid metabolism
Molecular function	Acyltransferase Transferase
PTM	Isopeptide bond Ubl conjugation
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	fatty acid biosynthetic process Inferred from electronic annotation. Source: UniProtKB-UniPathway
Molecular_function	[acyl-carrier-protein] S-malonyltransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 290	290	Malonyl CoA-acyl carrier protein transacylase		PRO_0000396808
Regions
	Compositional bias	190 – 193	4	Poly-Ala
Sites
	Active site	75	1	By similarity
	Active site	178	1	By similarity
Amino acid modifications
	Cross-link	157		Isoglutamyl lysine isopeptide (Lys-Gln) (interchain with Q-Cter in protein Pup)

Sequences

	Sequence		Length	Mass (Da)
	A0R0B2 [UniParc]. Last modified January 9, 2007. Version 1. Checksum: 06BFFF38EEDC5B69	FASTA	290	29,676
	10 20 30 40 50 60 MLTPWLELPG AADRLAAWSQ ISGLDLTTLG TTATAEEITD TAVTQPLVVA ATLLAHEELT 70 80 90 100 110 120 KRGHSAAETI VAGHSVGEIA AYAIAGVISA DDAVKLAATR GAEMAKACAV EPTGMAAVLG 130 140 150 160 170 180 GDEAEVLARL EALDLVPANR NAAGQIVAAG AVAALDKLAE DPPAKARVRK LATAGAFHTH 190 200 210 220 230 240 YMASALDGYA AAAQSVTTSE PTATLLSNAD GQPVASAADA MEKLVAQLTK PVRWDLCTAT 250 260 270 280 290 LRDRFQNAES AGIVEFPPAG TLVGIAKREL KGTPTRAIKS PEDLDGLDQL

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	Fleischmann R.D., Dodson R.J., Haft D.H., Merkel J.S., Nelson W.C., Fraser C.M. Submitted (OCT-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 700084 / mc(2)155.
[2]	"Interrupted coding sequences in Mycobacterium smegmatis: authentic mutations or sequencing errors?" Deshayes C., Perrodou E., Gallien S., Euphrasie D., Schaeffer C., Van-Dorsselaer A., Poch O., Lecompte O., Reyrat J.-M. Genome Biol. 8:R20.1-R20.9(2007) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 700084 / mc(2)155.
[3]	"Ortho-proteogenomics: multiple proteomes investigation through orthology and a new MS-based protocol." Gallien S., Perrodou E., Carapito C., Deshayes C., Reyrat J.-M., Van Dorsselaer A., Poch O., Schaeffer C., Lecompte O. Genome Res. 19:128-135(2009) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 700084 / mc(2)155.
[4]	"Expansion of the mycobacterial 'PUPylome'." Watrous J., Burns K., Liu W.T., Patel A., Hook V., Bafna V., Barry C.E. III, Bark S., Dorrestein P.C. Mol. Biosyst. 6:376-385(2010) [PubMed] [Europe PMC] [Abstract] Cited for: PUPYLATION AT LYS-157, IDENTIFICATION BY MASS SPECTROMETRY.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000480 Genomic DNA. Translation: ABK74111.1. CP001663 Genomic DNA. Translation: AFP40681.1. Different initiation.
RefSeq	YP_006568976.1. NC_018289.1. YP_888600.1. NC_008596.1.
3D structure databases
ProteinModelPortal	A0R0B2.
SMR	A0R0B2. Positions 1-290.
ModBase	Search...
Protein-protein interaction databases
STRING	246196.MSMEG_4325.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	ABK74111; ABK74111; MSMEG_4325.
GeneID	13430741. 4536190.
KEGG	msg:MSMEI_4225. msm:MSMEG_4325.
PATRIC	18081005. VBIMycSme59918_4243.
Phylogenomic databases
eggNOG	COG0331.
HOGENOM	HOG000036505.
KO	K00645.
OMA	WPANVNG.
ProtClustDB	CLSK2530285.
Enzyme and pathway databases
BioCyc	MSME246196:GJ4Y-4324-MONOMER.
UniPathway	UPA00094.
Family and domain databases
Gene3D	3.40.366.10. 2 hits.
InterPro	IPR001227. Ac_transferase_dom. IPR014043. Acyl_transferase. IPR016035. Acyl_Trfase/lysoPLipase. IPR016036. Malonyl_transacylase_ACP-bd. [Graphical view]
Pfam	PF00698. Acyl_transf_1. 1 hit. [Graphical view]
SUPFAM	SSF52151. Acyl_Trfase/lysoPlipase. 1 hit. SSF55048. Malonyl_transacylase_ACP-bd. 1 hit.
ProtoNet	Search...

Entry information

Entry name

FABD_MYCS2

Accession

Primary (citable) accession number: A0R0B2
Secondary accession number(s): I7GBV9

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 10, 2010
Last sequence update:	January 9, 2007
Last modified:	May 1, 2013
This is version 47 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program