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A0R0B0 (ODP1_MYCS2) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 58. Feed History...

Clusters with 100%, 90%, 50% identity | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry infoCustomize order

Names and origin

Protein namesRecommended name:
Pyruvate dehydrogenase E1 component

Short name=PDH E1 component
EC=1.2.4.1
Gene names
Name:aceE
Ordered Locus Names:MSMEG_4323, MSMEI_4223
OrganismMycobacterium smegmatis (strain ATCC 700084 / mc(2)155) [Reference proteome] [HAMAP]
Taxonomic identifier246196 [NCBI]
Taxonomic lineageBacteriaActinobacteriaActinobacteridaeActinomycetalesCorynebacterineaeMycobacteriaceaeMycobacterium

Protein attributes

Sequence length929 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Component of the pyruvate dehydrogenase (PDH) complex, that catalyzes the overall conversion of pyruvate to acetyl-CoA and CO2. AceE has reductase activity with pyruvate but does not react with 2-oxoglutarate By similarity.

Catalytic activity

Pyruvate + [dihydrolipoyllysine-residue acetyltransferase] lipoyllysine = [dihydrolipoyllysine-residue acetyltransferase] S-acetyldihydrolipoyllysine + CO2.

Cofactor

Magnesium By similarity.

Thiamine pyrophosphate By similarity.

Subunit structure

Homodimer By similarity. Part of the PDH complex, consisting of multiple copies of AceE (E1), DlaT (E2) and Lpd (E3) By similarity.

Ontologies

Keywords
   Biological processGlycolysis
   LigandMagnesium
Pyruvate
Thiamine pyrophosphate
   Molecular functionOxidoreductase
   PTMIsopeptide bond
Ubl conjugation
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processglycolytic process

Inferred from electronic annotation. Source: UniProtKB-KW

   Molecular_functionpyruvate dehydrogenase (acetyl-transferring) activity

Inferred from electronic annotation. Source: UniProtKB-EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 929929Pyruvate dehydrogenase E1 component
PRO_0000396809

Amino acid modifications

Cross-link375Isoglutamyl lysine isopeptide (Lys-Gln) (interchain with Q-Cter in protein Pup)

Sequences

Sequence LengthMass (Da)Tools
A0R0B0 [UniParc].

Last modified January 9, 2007. Version 1.
Checksum: 5CB137A1D69BFB23

FASTA929103,077
        10         20         30         40         50         60 
MTTEFVRQDL AQNSSTAAEP DRVRVIREGV ASYLPDIDTE ETAEWLESFD ELLERSGPAR 

        70         80         90        100        110        120 
ARYLMLRLLE RAGEQRVAIP ALTSTDYVNT IPTELEPWFP GDEDVERRYR AWIRWNAAIM 

       130        140        150        160        170        180 
VHRAQRPGVG VGGHISTYAS SATLYEVGFN HFFRGKSHPG GGDHVFIQGH ASPGIYARAF 

       190        200        210        220        230        240 
LEGRLTTDQL DGFRQEHSHS GGGLPSYPHP RLMPDFWEFP TVSMGLGPMN AIYQARFNHY 

       250        260        270        280        290        300 
LHDRGIKDTS DQHVWAFLGD GEMDEPESRG LIQVAANEAL DNLTFVINCN LQRLDGPVRG 

       310        320        330        340        350        360 
NGKIIQELES FFRGAGWNVI KVVWGREWDV LLHADRDGAL VNLMNSTPDG DYQTYKANDG 

       370        380        390        400        410        420 
AYVRDHFFGR DPRTKALVAD MSDQEIWNLK RGGHDYRKVY AAYRAAMEHK GQPTVILAKT 

       430        440        450        460        470        480 
IKGYTLGQHF EGRNATHQMK KLALEDLKNF RDVTRVPVSD AQLEEDPYLP PYYHPGPEAP 

       490        500        510        520        530        540 
EIRYLLERRR ALGGFVPSRR TKSKPLALPG SDTYKALKKG SGSQAVATTM ATVRTFKELL 

       550        560        570        580        590        600 
RDKNIGPRIV PIIPDEARTF GMDSWFPSLK IYNRNGQLYT SVDSELMLAY KESEVGQILH 

       610        620        630        640        650        660 
EGINEAGSTS SFTAVGTSYS THDEPMIPIY IFYSMFGFQR TGDGLWAAAD QMARGFVLGA 

       670        680        690        700        710        720 
TAGRTTLTGE GLQHADGHSL LLASTNPAAV TYDPAFAYEI AHIIESGLQR MYGEDPENVF 

       730        740        750        760        770        780 
FYLTIYNEPY QQPAEPENLD VEALLKGLYL YRPAPEKRAK SAQILASGVA MPEALRAADL 

       790        800        810        820        830        840 
LASDWDVAAD VWSVTSWGEL NREGVAIEKH RLRHPDEPAG TPHVTSALAD AAGPVIAVSD 

       850        860        870        880        890        900 
WMRAVPEQIR PWVPGTYVTL GTDGFGFSDT RPAARRYFNT DAESVVVAVL QGLARDGEID 

       910        920 
ASVAAQAAEQ YRIDDVSAAG VSYADTGSA 

« Hide

References

« Hide 'large scale' references
[1]Fleischmann R.D., Dodson R.J., Haft D.H., Merkel J.S., Nelson W.C., Fraser C.M.
Submitted (OCT-2006) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 700084 / mc(2)155.
[2]"Interrupted coding sequences in Mycobacterium smegmatis: authentic mutations or sequencing errors?"
Deshayes C., Perrodou E., Gallien S., Euphrasie D., Schaeffer C., Van-Dorsselaer A., Poch O., Lecompte O., Reyrat J.-M.
Genome Biol. 8:R20.1-R20.9(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 700084 / mc(2)155.
[3]"Ortho-proteogenomics: multiple proteomes investigation through orthology and a new MS-based protocol."
Gallien S., Perrodou E., Carapito C., Deshayes C., Reyrat J.-M., Van Dorsselaer A., Poch O., Schaeffer C., Lecompte O.
Genome Res. 19:128-135(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 700084 / mc(2)155.
[4]"Expansion of the mycobacterial 'PUPylome'."
Watrous J., Burns K., Liu W.T., Patel A., Hook V., Bafna V., Barry C.E. III, Bark S., Dorrestein P.C.
Mol. Biosyst. 6:376-385(2010) [PubMed] [Europe PMC] [Abstract]
Cited for: PUPYLATION AT LYS-375, IDENTIFICATION BY MASS SPECTROMETRY.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP000480 Genomic DNA. Translation: ABK72760.1.
CP001663 Genomic DNA. Translation: AFP40679.1.
RefSeqYP_006568974.1. NC_018289.1.
YP_888598.1. NC_008596.1.

3D structure databases

ProteinModelPortalA0R0B0.
SMRA0R0B0. Positions 85-916.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING246196.MSMEG_4323.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaABK72760; ABK72760; MSMEG_4323.
AFP40679; AFP40679; MSMEI_4223.
GeneID4531364.
KEGGmsg:MSMEI_4223.
msm:MSMEG_4323.
PATRIC18081001. VBIMycSme59918_4241.

Phylogenomic databases

eggNOGCOG2609.
HOGENOMHOG000115215.
KOK00163.
OMALVHLMNT.
OrthoDBEOG6BW4TW.

Enzyme and pathway databases

BioCycMSME246196:GJ4Y-4322-MONOMER.

Family and domain databases

Gene3D3.40.50.920. 1 hit.
3.40.50.970. 2 hits.
InterProIPR004660. 2-oxoA_DH_E1.
IPR029061. THDP-binding.
IPR009014. Transketo_C/Pyr-ferredox_oxred.
IPR005474. Transketolase_N.
[Graphical view]
PfamPF00456. Transketolase_N. 1 hit.
[Graphical view]
PIRSFPIRSF000156. Pyruvate_dh_E1. 1 hit.
SUPFAMSSF52518. SSF52518. 2 hits.
SSF52922. SSF52922. 1 hit.
TIGRFAMsTIGR00759. aceE. 1 hit.
ProtoNetSearch...

Entry information

Entry nameODP1_MYCS2
AccessionPrimary (citable) accession number: A0R0B0
Secondary accession number(s): I7GD92
Entry history
Integrated into UniProtKB/Swiss-Prot: August 10, 2010
Last sequence update: January 9, 2007
Last modified: July 9, 2014
This is version 58 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program