A0R0B0 (ODP1_MYCS2) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Pyruvate dehydrogenase E1 component
Short name=PDH E1 component
EC=1.2.4.1

Gene names

Name:	aceE
Ordered Locus Names:	MSMEG_4323, MSMEI_4223

Organism

Mycobacterium smegmatis (strain ATCC 700084 / mc(2)155) [Reference proteome] [HAMAP]

Taxonomic identifier

246196 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Actinobacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Corynebacterineae › Mycobacteriaceae › Mycobacterium › Mycobacterium smegmatis

Protein attributes

Sequence length	929 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Component of the pyruvate dehydrogenase (PDH) complex, that catalyzes the overall conversion of pyruvate to acetyl-CoA and CO2. AceE has reductase activity with pyruvate but does not react with 2-oxoglutarate By similarity.
Catalytic activity	Pyruvate + [dihydrolipoyllysine-residue acetyltransferase] lipoyllysine = [dihydrolipoyllysine-residue acetyltransferase] S-acetyldihydrolipoyllysine + CO2.
Cofactor	Magnesium By similarity. Thiamine pyrophosphate By similarity.
Subunit structure	Homodimer By similarity. Makes part of the PDH complex, consisting of multiple copies of AceE (E1), DlaT (E2) and Lpd (E3) By similarity.

Ontologies

Keywords
Biological process	Glycolysis
Ligand	Magnesium Pyruvate Thiamine pyrophosphate
Molecular function	Oxidoreductase
PTM	Isopeptide bond Ubl conjugation
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	glycolysis Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	pyruvate dehydrogenase (acetyl-transferring) activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 929	929	Pyruvate dehydrogenase E1 component		PRO_0000396809
Amino acid modifications
	Cross-link	375		Isoglutamyl lysine isopeptide (Lys-Gln) (interchain with Q-Cter in protein Pup)

Sequences

	Sequence		Length	Mass (Da)
	A0R0B0 [UniParc]. Last modified January 9, 2007. Version 1. Checksum: 5CB137A1D69BFB23	FASTA	929	103,077
	10 20 30 40 50 60 MTTEFVRQDL AQNSSTAAEP DRVRVIREGV ASYLPDIDTE ETAEWLESFD ELLERSGPAR 70 80 90 100 110 120 ARYLMLRLLE RAGEQRVAIP ALTSTDYVNT IPTELEPWFP GDEDVERRYR AWIRWNAAIM 130 140 150 160 170 180 VHRAQRPGVG VGGHISTYAS SATLYEVGFN HFFRGKSHPG GGDHVFIQGH ASPGIYARAF 190 200 210 220 230 240 LEGRLTTDQL DGFRQEHSHS GGGLPSYPHP RLMPDFWEFP TVSMGLGPMN AIYQARFNHY 250 260 270 280 290 300 LHDRGIKDTS DQHVWAFLGD GEMDEPESRG LIQVAANEAL DNLTFVINCN LQRLDGPVRG 310 320 330 340 350 360 NGKIIQELES FFRGAGWNVI KVVWGREWDV LLHADRDGAL VNLMNSTPDG DYQTYKANDG 370 380 390 400 410 420 AYVRDHFFGR DPRTKALVAD MSDQEIWNLK RGGHDYRKVY AAYRAAMEHK GQPTVILAKT 430 440 450 460 470 480 IKGYTLGQHF EGRNATHQMK KLALEDLKNF RDVTRVPVSD AQLEEDPYLP PYYHPGPEAP 490 500 510 520 530 540 EIRYLLERRR ALGGFVPSRR TKSKPLALPG SDTYKALKKG SGSQAVATTM ATVRTFKELL 550 560 570 580 590 600 RDKNIGPRIV PIIPDEARTF GMDSWFPSLK IYNRNGQLYT SVDSELMLAY KESEVGQILH 610 620 630 640 650 660 EGINEAGSTS SFTAVGTSYS THDEPMIPIY IFYSMFGFQR TGDGLWAAAD QMARGFVLGA 670 680 690 700 710 720 TAGRTTLTGE GLQHADGHSL LLASTNPAAV TYDPAFAYEI AHIIESGLQR MYGEDPENVF 730 740 750 760 770 780 FYLTIYNEPY QQPAEPENLD VEALLKGLYL YRPAPEKRAK SAQILASGVA MPEALRAADL 790 800 810 820 830 840 LASDWDVAAD VWSVTSWGEL NREGVAIEKH RLRHPDEPAG TPHVTSALAD AAGPVIAVSD 850 860 870 880 890 900 WMRAVPEQIR PWVPGTYVTL GTDGFGFSDT RPAARRYFNT DAESVVVAVL QGLARDGEID 910 920 ASVAAQAAEQ YRIDDVSAAG VSYADTGSA

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	Fleischmann R.D., Dodson R.J., Haft D.H., Merkel J.S., Nelson W.C., Fraser C.M. Submitted (OCT-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 700084 / mc(2)155.
[2]	"Interrupted coding sequences in Mycobacterium smegmatis: authentic mutations or sequencing errors?" Deshayes C., Perrodou E., Gallien S., Euphrasie D., Schaeffer C., Van-Dorsselaer A., Poch O., Lecompte O., Reyrat J.-M. Genome Biol. 8:R20.1-R20.9(2007) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 700084 / mc(2)155.
[3]	"Ortho-proteogenomics: multiple proteomes investigation through orthology and a new MS-based protocol." Gallien S., Perrodou E., Carapito C., Deshayes C., Reyrat J.-M., Van Dorsselaer A., Poch O., Schaeffer C., Lecompte O. Genome Res. 19:128-135(2009) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 700084 / mc(2)155.
[4]	"Expansion of the mycobacterial 'PUPylome'." Watrous J., Burns K., Liu W.T., Patel A., Hook V., Bafna V., Barry C.E. III, Bark S., Dorrestein P.C. Mol. Biosyst. 6:376-385(2010) [PubMed] [Europe PMC] [Abstract] Cited for: PUPYLATION AT LYS-375, IDENTIFICATION BY MASS SPECTROMETRY.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000480 Genomic DNA. Translation: ABK72760.1. CP001663 Genomic DNA. Translation: AFP40679.1.
RefSeq	YP_006568974.1. NC_018289.1. YP_888598.1. NC_008596.1.
3D structure databases
ProteinModelPortal	A0R0B0.
SMR	A0R0B0. Positions 85-916.
ModBase	Search...
Protein-protein interaction databases
STRING	246196.MSMEG_4323.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	ABK72760; ABK72760; MSMEG_4323.
GeneID	13430739. 4531364.
KEGG	msg:MSMEI_4223. msm:MSMEG_4323.
PATRIC	18081001. VBIMycSme59918_4241.
Phylogenomic databases
eggNOG	COG2609.
HOGENOM	HOG000115215.
KO	K00163.
OMA	DAVECEE.
ProtClustDB	PRK09405.
Enzyme and pathway databases
BioCyc	MSME246196:GJ4Y-4322-MONOMER.
Family and domain databases
Gene3D	3.40.50.920. 1 hit.
InterPro	IPR004660. 2-oxoA_DH_E1. IPR009014. Transketo_C/Pyr-ferredox_oxred. IPR015941. Transketolase-like_C. IPR005474. Transketolase_N. [Graphical view]
Pfam	PF00456. Transketolase_N. 1 hit. [Graphical view]
PIRSF	PIRSF000156. Pyruvate_dh_E1. 1 hit.
SUPFAM	SSF52922. Transketo_C_like. 1 hit.
TIGRFAMs	TIGR00759. aceE. 1 hit.
ProtoNet	Search...

Entry information

Entry name

ODP1_MYCS2

Accession

Primary (citable) accession number: A0R0B0
Secondary accession number(s): I7GD92

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 10, 2010
Last sequence update:	January 9, 2007
Last modified:	May 1, 2013
This is version 52 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program