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A0R0B0

- ODP1_MYCS2

UniProt

A0R0B0 - ODP1_MYCS2

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Protein
Pyruvate dehydrogenase E1 component
Gene
aceE, MSMEG_4323, MSMEI_4223
Organism
Mycobacterium smegmatis (strain ATCC 700084 / mc(2)155)
Status
Reviewed - Annotation score: 2 out of 5 - Experimental evidence at protein leveli

Functioni

Component of the pyruvate dehydrogenase (PDH) complex, that catalyzes the overall conversion of pyruvate to acetyl-CoA and CO2. AceE has reductase activity with pyruvate but does not react with 2-oxoglutarate By similarity.

Catalytic activityi

Pyruvate + [dihydrolipoyllysine-residue acetyltransferase] lipoyllysine = [dihydrolipoyllysine-residue acetyltransferase] S-acetyldihydrolipoyllysine + CO2.

Cofactori

Magnesium By similarity.
Thiamine pyrophosphate By similarity.

GO - Molecular functioni

  1. pyruvate dehydrogenase (acetyl-transferring) activity Source: UniProtKB-EC

GO - Biological processi

  1. glycolytic process Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Glycolysis

Keywords - Ligandi

Magnesium, Pyruvate, Thiamine pyrophosphate

Enzyme and pathway databases

BioCyciMSME246196:GJ4Y-4322-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Pyruvate dehydrogenase E1 component (EC:1.2.4.1)
Short name:
PDH E1 component
Gene namesi
Name:aceE
Ordered Locus Names:MSMEG_4323, MSMEI_4223
OrganismiMycobacterium smegmatis (strain ATCC 700084 / mc(2)155)
Taxonomic identifieri246196 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaActinobacteridaeActinomycetalesCorynebacterineaeMycobacteriaceaeMycobacterium
ProteomesiUP000000757: Chromosome, UP000006158: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 929929Pyruvate dehydrogenase E1 component
PRO_0000396809Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Cross-linki375 – 375Isoglutamyl lysine isopeptide (Lys-Gln) (interchain with Q-Cter in protein Pup)

Keywords - PTMi

Isopeptide bond, Ubl conjugation

Interactioni

Subunit structurei

Homodimer By similarity. Part of the PDH complex, consisting of multiple copies of AceE (E1), DlaT (E2) and Lpd (E3) By similarity.

Protein-protein interaction databases

STRINGi246196.MSMEG_4323.

Structurei

3D structure databases

ProteinModelPortaliA0R0B0.
SMRiA0R0B0. Positions 85-916.

Family & Domainsi

Phylogenomic databases

eggNOGiCOG2609.
HOGENOMiHOG000115215.
KOiK00163.
OMAiLVHLMNT.
OrthoDBiEOG6BW4TW.

Family and domain databases

Gene3Di3.40.50.920. 1 hit.
3.40.50.970. 2 hits.
InterProiIPR004660. 2-oxoA_DH_E1.
IPR029061. THDP-binding.
IPR009014. Transketo_C/Pyr-ferredox_oxred.
IPR005474. Transketolase_N.
[Graphical view]
PfamiPF00456. Transketolase_N. 1 hit.
[Graphical view]
PIRSFiPIRSF000156. Pyruvate_dh_E1. 1 hit.
SUPFAMiSSF52518. SSF52518. 2 hits.
SSF52922. SSF52922. 1 hit.
TIGRFAMsiTIGR00759. aceE. 1 hit.

Sequencei

Sequence statusi: Complete.

A0R0B0-1 [UniParc]FASTAAdd to Basket

« Hide

MTTEFVRQDL AQNSSTAAEP DRVRVIREGV ASYLPDIDTE ETAEWLESFD    50
ELLERSGPAR ARYLMLRLLE RAGEQRVAIP ALTSTDYVNT IPTELEPWFP 100
GDEDVERRYR AWIRWNAAIM VHRAQRPGVG VGGHISTYAS SATLYEVGFN 150
HFFRGKSHPG GGDHVFIQGH ASPGIYARAF LEGRLTTDQL DGFRQEHSHS 200
GGGLPSYPHP RLMPDFWEFP TVSMGLGPMN AIYQARFNHY LHDRGIKDTS 250
DQHVWAFLGD GEMDEPESRG LIQVAANEAL DNLTFVINCN LQRLDGPVRG 300
NGKIIQELES FFRGAGWNVI KVVWGREWDV LLHADRDGAL VNLMNSTPDG 350
DYQTYKANDG AYVRDHFFGR DPRTKALVAD MSDQEIWNLK RGGHDYRKVY 400
AAYRAAMEHK GQPTVILAKT IKGYTLGQHF EGRNATHQMK KLALEDLKNF 450
RDVTRVPVSD AQLEEDPYLP PYYHPGPEAP EIRYLLERRR ALGGFVPSRR 500
TKSKPLALPG SDTYKALKKG SGSQAVATTM ATVRTFKELL RDKNIGPRIV 550
PIIPDEARTF GMDSWFPSLK IYNRNGQLYT SVDSELMLAY KESEVGQILH 600
EGINEAGSTS SFTAVGTSYS THDEPMIPIY IFYSMFGFQR TGDGLWAAAD 650
QMARGFVLGA TAGRTTLTGE GLQHADGHSL LLASTNPAAV TYDPAFAYEI 700
AHIIESGLQR MYGEDPENVF FYLTIYNEPY QQPAEPENLD VEALLKGLYL 750
YRPAPEKRAK SAQILASGVA MPEALRAADL LASDWDVAAD VWSVTSWGEL 800
NREGVAIEKH RLRHPDEPAG TPHVTSALAD AAGPVIAVSD WMRAVPEQIR 850
PWVPGTYVTL GTDGFGFSDT RPAARRYFNT DAESVVVAVL QGLARDGEID 900
ASVAAQAAEQ YRIDDVSAAG VSYADTGSA 929
Length:929
Mass (Da):103,077
Last modified:January 9, 2007 - v1
Checksum:i5CB137A1D69BFB23
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000480 Genomic DNA. Translation: ABK72760.1.
CP001663 Genomic DNA. Translation: AFP40679.1.
RefSeqiYP_006568974.1. NC_018289.1.
YP_888598.1. NC_008596.1.

Genome annotation databases

EnsemblBacteriaiABK72760; ABK72760; MSMEG_4323.
AFP40679; AFP40679; MSMEI_4223.
GeneIDi4531364.
KEGGimsg:MSMEI_4223.
msm:MSMEG_4323.
PATRICi18081001. VBIMycSme59918_4241.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000480 Genomic DNA. Translation: ABK72760.1 .
CP001663 Genomic DNA. Translation: AFP40679.1 .
RefSeqi YP_006568974.1. NC_018289.1.
YP_888598.1. NC_008596.1.

3D structure databases

ProteinModelPortali A0R0B0.
SMRi A0R0B0. Positions 85-916.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 246196.MSMEG_4323.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ABK72760 ; ABK72760 ; MSMEG_4323 .
AFP40679 ; AFP40679 ; MSMEI_4223 .
GeneIDi 4531364.
KEGGi msg:MSMEI_4223.
msm:MSMEG_4323.
PATRICi 18081001. VBIMycSme59918_4241.

Phylogenomic databases

eggNOGi COG2609.
HOGENOMi HOG000115215.
KOi K00163.
OMAi LVHLMNT.
OrthoDBi EOG6BW4TW.

Enzyme and pathway databases

BioCyci MSME246196:GJ4Y-4322-MONOMER.

Family and domain databases

Gene3Di 3.40.50.920. 1 hit.
3.40.50.970. 2 hits.
InterProi IPR004660. 2-oxoA_DH_E1.
IPR029061. THDP-binding.
IPR009014. Transketo_C/Pyr-ferredox_oxred.
IPR005474. Transketolase_N.
[Graphical view ]
Pfami PF00456. Transketolase_N. 1 hit.
[Graphical view ]
PIRSFi PIRSF000156. Pyruvate_dh_E1. 1 hit.
SUPFAMi SSF52518. SSF52518. 2 hits.
SSF52922. SSF52922. 1 hit.
TIGRFAMsi TIGR00759. aceE. 1 hit.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. Fleischmann R.D., Dodson R.J., Haft D.H., Merkel J.S., Nelson W.C., Fraser C.M.
    Submitted (OCT-2006) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 700084 / mc(2)155.
  2. "Interrupted coding sequences in Mycobacterium smegmatis: authentic mutations or sequencing errors?"
    Deshayes C., Perrodou E., Gallien S., Euphrasie D., Schaeffer C., Van-Dorsselaer A., Poch O., Lecompte O., Reyrat J.-M.
    Genome Biol. 8:R20.1-R20.9(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 700084 / mc(2)155.
  3. "Ortho-proteogenomics: multiple proteomes investigation through orthology and a new MS-based protocol."
    Gallien S., Perrodou E., Carapito C., Deshayes C., Reyrat J.-M., Van Dorsselaer A., Poch O., Schaeffer C., Lecompte O.
    Genome Res. 19:128-135(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 700084 / mc(2)155.
  4. Cited for: PUPYLATION AT LYS-375, IDENTIFICATION BY MASS SPECTROMETRY.

Entry informationi

Entry nameiODP1_MYCS2
AccessioniPrimary (citable) accession number: A0R0B0
Secondary accession number(s): I7GD92
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 10, 2010
Last sequence update: January 9, 2007
Last modified: July 9, 2014
This is version 58 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

External Data

Dasty 3

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